990 resultados para 206-1256


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利用有限元数值模拟方法研究了深水细长柔性立管在正弦形式涡激升力作用下的动响应,并通过量纲分析结合函数拟合方式给出了判定立管涡激振动响应形式的无量纲参数的表达式和临界值,该参数与系统阻尼(包括流体阻尼和结构阻尼)、结构模态阶数及结构长度等有关。在实际海洋工程中,当设计或监测人员在预测立管的涡激振动响应时,可以先利用本文方法判定振动响应形式,然后根据响应形式选择合适的预报模型。

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鞑靼荞麦是我国特有的农业产品,具有抗寒耐旱特性和较高的营养保健功能。荞麦的开花习性及遗传特点导致其人工杂交授粉难以成功,这成为荞麦杂交育种难以获得突破的重要原因。因此利用转基因技术导入有益基因有可能成为荞麦遗传改良的新途径,而再生及转化体系的建立是开展转基因研究的基础。 本文研究了苗龄、外植体、几种激素配比对鞑靼荞麦(Fagopyrum tataricum Gaertn.)离体培养的影响,初步建立了鞑靼荞麦离体再生体系。结果表明,鞑靼荞麦离体再生的最佳取材时间为苗龄6-8d;诱导愈伤组织的最适培养基为MS+2.0 mg/L 2,4-D+1.5 mg/L 6-BA,子叶诱愈率达75%左右,下胚轴的可高达86.62%;愈伤组织分化的最适培养基为MS 0.1mg/L IAA+2.0mg/L 6-BA+1.0 mg/L KT+0.5mg/L TDZ,下胚轴的分化率可达9.52%。下胚轴的诱愈率与分化率均高于子叶,更适于离体再生培养。培养基中加入AgNO3后,能有效降低褐化率。生根最适培养基为含有0.5mg/L NAA的1/2MS培养基,生根率在50%左右。TDZ在诱导鞑靼荞麦的愈伤组织分化出芽的过程中起到明显的促进作用,可提高分化率约20%。 在上述研究基础上,本文还对鞑靼荞麦的遗传转化体系进行了探索性研究。分别利用根癌农杆菌(Agrobacterium tumefaciens)介导法和微粒轰击法(基因枪法)对黑水苦荞下胚轴进行遗传转化。 在农杆菌介导的方法中,携带有质粒pCAMBIA2301的农杆菌菌株EHA105用于转化。载体质粒pCAMBIA2301包含有gus和npt-II 基因, 并受35s启动子驱动。研究结果表明,在侵染方式选择上,浸泡方式比吸打方式更有效,根癌农杆菌侵染的较适浓度为OD600=0.5,共培养3天,恢复培养7天,能检测到gus基因的表达。 基因枪法使用质粒pBI121,同样包含有gus和npt-II基因, 并受CaMV35s 启动子驱动。轰击距离为9cm较合适,甘露醇前处理在本研究中未表现出明显优势。 两种转化方法比较,基因枪法比农杆菌介导法更快速有效。 本研究为进一步的遗传操作研究打下基础。 Tartary buckwheat (Fagopyrum tataricum Gaertn.), the traditional and unique agricultural product of China, is a kind of crop with strong drought and cold tolerance, abundant nutrition and high medical value. Artificial hybridization is hard in buckwheat because of its flowering habits and genetic characteristics, which leads to no breakthrough in tartary buckwheat breeding. However, biotechnological approaches, especially genetic transformation for the direct introduction of good genes into tartary buckwheat for quality improvement, hold great promise. In this study, we established tartary buckwheat regeneration system in vitro. It is the foundation for genetic manipulation of this crop. The effects of seedling age, hypocotyl and cotyledon as explants, and proportions of several growth regulators were tested in tissue culture of tartary buckwheat for establishing its in vitro regeneration system. The results showed that the best seedling age for callus induction was 6 to 8 days. On the MS medium containing 2.0mg/L 2, 4-D and 1.5mg/L 6-BA, the induction rate of callus from hypocotyls was up to 86.62%, while from cotyledons was about 75%. The suitable shooting medium was the MS medium+0.1mg/L IAA+2.0mg/L 6-BA+1.0 mg/L KT+0.5mg/L TDZ, and the shooting rate from hypocotyls was 9.52%. The callus induction and shooting rates were higher from hypocotyls than from cotyledons. Browning reduced when the medium mixed with AgNO3. Half strength MS supplemented with 0.5mg/L NAA was the best for rooting, the rate was around 50% after 30 days culture. TDZ can accelerate the shoot differentiation distinctively, and it could improve the shooting rate nearly 20%. On the base of above, the explorative research of the genetic transformation in tartary buckwheat was done. In the study, hypocotyls from Heishui tartary buckwheat were transformed by Agrobacterium-mediated method and microprojectile bombardment method (gene-gun), comparatively. In Agrobacterium-mediated method, a disarmed Agrobacterium tumefaciens strain EHA105 harboring plasmid pCAMBIA2301 was used. The vector pCAMBIA2301 contains gus and npt-II genes, driven by CaMV35s promoter. The results showed that the appropriate concentration of Agrobacterium tumefaciens for infecting was OD600=0.5, and co-culture time was 3d. Seven days later after coculture, GUS expression could be tested. In particle bombardment transformation, plasmid pBI121 was used. pBI121 also contains gus and npt-II genes, driven by 35s promoter. Hypocotyls pretreated with mannitol, no effect was observed, and the suitable distance of bombardment is 9cm. Comparing with Agrobacterium-mediated method, gene-gun method is more convenient and effective. All above results could be a basic work for further study in tartary buckwheat transformation.

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Based on Monte Carlo method the microscopic gas-phase adsorption chromatographic behaviors of volatile chemical compounds were simulated,and the migration process of those compounds followed by the carrier gases along the chromatographic surface and the process of adsorption-desorption were also described.The programs have been compiled to simulate the processes of thermochromatography and isothermal chromatography with different experimental conditions and properties.The calculated results agree well with ...中文摘要:用蒙特卡罗方法模拟了易挥发化合物的气相色谱微观动力学行为,描述了化合物随气流在固体色谱柱表面的迁移以及吸附-解吸等过程。按气相色谱微观动力学模型编译的计算机程序模拟了超重元素化合物在等温色谱及热色谱的动力学过程,并根据不同实验条件进行了大量计算。计算结果与实验数据较为吻合。讨论了超重元素的半衰期、吸附态的周期、载气流量以及化合物的质量密度等对实验结果的影响及该理论模型的优点和待改进的地方。

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中国科学院近代物理研究所成功研制了DG系列中频变压器型工业电子加速器,其中DG-2.5型加速器能量范围1.0–2.5MeV,最大束流功率90kW,束流扫描有效宽度1000mm,适用于各种辐照领域;DG-1.2型能量范围0.8–1.2MeV,最大功率50kW;这两种型号加速器均通过了长时间测试,工作稳定、故障率低,采用计算机自动控制,并可与束下传动系统连锁,完全达到了工业应用的标准。

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本文主要阐述了新型的软件无线电I、Q两路同步采样方法,主要利用了平方律部件、一阶环路滤波器和NCO来对I、Q两路采样时间差的进行闭环控制,实现了实时同步采样,降低了对AD芯片采样速率要求,同时也为后续的基带信号处理提供了方便。该设计主要用于兰州重离子加速器冷却存贮环的主环(CSRm)的重离子束团踢轨(Kicker System)的精确控制。

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在210MeV的束流能量下,利用重离子熔合蒸发反应146Nd(35Cl,5nγ)176Ir产生具有β+/EC衰变性质的核素176Ir,由氦喷嘴快速带传输系统将反应产物传送到低本底区进行测量.经过实验数据的离线处理分析,对早先发表的176Ir衰变γ跃迁进行确认的同时又发现了3个新能级和10条新的γ射线, 丰富了176Os核的低位激发态能级纲图.并根据典型γ射线的衰变时间谱建议了176Ir核的一个长寿命的低自旋同核异能态.

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介绍了基于MSC1210单片机设计的一个电源控制模块,它通过RS-485总线实现与上位机的通信,来控制电源的开关、调节和状态获取等,实践表明,该模块具有较高的可靠性、实时性和适应性。

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总结了兰州放射性束实验小组在兰州放射性次级束流线上近几年来利用产生的放射性束流 ,轰击Si或C等靶子 ,测量它的反应总截面 .并利用经验公式将这些结果归一到相同的能量和靶子 ,与其相邻的核相比较 ,发现了9C ,11Be ,14 Be ,8B ,14 B和12 N等核素的反应总截面值有奇异增大的现象 .利用微观的Glauber模型进行了计算 ,对有奇异结构的核采用核芯加价核子的密度分布形式 ,理论计算和实验结果符合得很好 ,可以给出奇异核的弥散的密度分布 .

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对有限力程小液滴模型计算的质子数Z=101—130超重核同位素链核的基态性质进行了系统的分析,通过对这些同位素链中处于裂变稳定线上核的平均结合能、四极形变、单质子分离能、双质子分离能和α衰变能等性质的系统研究,显示了质子数Z=108,114,126和中子数N=162,180,196时壳幻数的存在,同时可以看到Z=127之后超重核的基态性质还需要进一步研究.