992 resultados para 10-PHENANTHROLINE-5


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本发明公开一种剥离型深海鱼皮胶原肽面膜及其制备方法。该方法以提取的深海鱼皮胶原多肽1~10份结合玫瑰纯露1~3份、海藻糖1~2份、菊糖0.5~1份为功能原料,以成膜剂10~30份、无机粉状物10~30份、保湿剂5~10份、油脂5~10份以及表面活性剂1~2份为辅助原料,原料中各组分均以重量计,经溶解、混合、搅拌、均质等工艺制得剥离型深海鱼皮胶原肽面膜。本发明的胶原多肽面膜不仅具有良好的清洁效果,而且可以补充肌肤营养、平整细纹、延缓衰老,是一种经济实用有效的美容护肤化妆品。

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本发明涉及环境科学或环境工程及水生生物实验研究用采样设备,特别是一种较浅水域环境及生物样品采集的采样装置。具体为包括撑架19固定架10、采样器2、风扇3、绳索8及插销7,撑架19两侧设有浮子5,带有采样器2的固定架10固接于撑架19的上方;在浮子5上安装有驱动其移动的风扇3,风扇3通过插销7与浮子5上的蓄电池9电连接;撑架19 的一侧固接有固定杆11,绳索8为两根,分别与采样器2及插销7相连接。本发明适用范围较广,其结构组成简单,操作便捷,可以应用于河道、海滩、池塘等较浅水域沉积物、水样及水体生物样品等的样品采集。而且本发明不需要大型动力设施及繁杂的日常维护,因此操作方便快捷,应用功能较强。

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运用蛋白电泳技术, 对云南普通马和矮型马群体的遗传多样性及其群众间遗传分化关系作了分析研究。44个遗传座位中有10个座位检测到多态性, 通过多态百分比、平均杂合度、平均等位基因的计算表明, 云南普通马和矮型马的遗传多样性较为丰富, 小区域内的群体存在着多样的遗传基因, 两种马在遗传上有一定差异。根据分子钟假说和相应的公式, 推算两者的分岐时间约为18.5万年。图1表2参12(金显谟)

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Two experiments were conducted to formulate and evaluate test diets using locally available ingredients to find out suitable diets for mono and polyculture of Macrobrachium rosenbergii in ponds. The first experiment was conducted from 1 July 2003 to 29 September 2003 in 12 experimental ponds each measuring 30 square meters behind the Fisheries Faculty Building, BAU campus, Mymensingh. Three experimental diets containing 30% protein were formulated using fish meal, meat and bone meal, mustard oilcake, sesame meal and rice bran and assigned to treatments 1, 2 and 3, respectively. A special shrimp feed (Starter-II) from Saudi-Bangla Fish Feed Ltd. was assigned to treatment 4 (Control). Each treatment had three replications. Juveniles of M. rosenbergii (2.90±0.81g) were stocked at the rate of 4/square meter. Prawns were fed three times daily at the rate of 15% of their body weight at the beginning, which was gradually reduced to 10% and 5% for the last two months. There was no significant (P>0.05) difference between the weight gains of prawns fed diets 1 and 4 (control), but they were significantly (P<0.05) higher than those of diets 2 and 3. The FCR values of diets ranged between 2.61 to 3.36 with diets 1 and 4 showing significantly (P<0.05) lower FCR values. The survival rate of prawns ranged between 68 to 78% with prawns fed diets 1 and 4 showing significantly higher survival rate. The production of prawn ranged from 921 to 1,428 kg/ha/90 days and diet 1 gave the highest production. Treatment 1 gave the highest net profit ofTk. 161,980/ha/90 days. The second experiment was conducted from December, 2003 to April, 2004 to see the growth of over wintered M. rosenbergii juveniles in polyculture with indigenous major carps (catla and rohu) in ponds using formulated diets. Three isoenergetic experimental diets formulated using fish meal, meat and bone meal, mustard oil cake, rice bran, wheat bran and molasses, and a shrimp feed 'Golda special feed' from Saudi-Bangla Fish Feed Ltd. were assigned to treatments 1, 2, 3 and 4 (control), respectively, each treatment had two replications. Eight experimental ponds each measuring 80 square meters in the Field Laboratory Complex of the Faculty of Fisheries, BAU campus, Mymensingh were used. The mean initial weights of M. rosenbergii, catla and rohu were 1.60±0.01, 30.0±0.09 and 25.0±0.08 g, respectively. A total of 160 fish and prawn (20,000/ha) were stocked in each pond at the ratio of 2:1:1 (prawn: catla: rohu). Fish were fed at the rate of 3-5% of their body weight. Prawns fed diet 1, 2 and 4 showed higher weight gains compared to diet 3. The weight gain of catla was significantly higher in T1 while in case of rohu was higher in T1 and T4, respectively. There were no significant differences (P>0.05) in the survival rate of fish as obtained from different treatments. The overall total fish production ranged from 2196 to 2679 kg/ha/5 months. The highest production and the highest profit (Tk. 56,531/ha/5 months) was obtained from T1 and the lowest (Tk. 24,932/ha/5 months) from T4.

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The total aerobic viable plate counts (TPCs) of skin, gills and intestine of newly caught oil sardine (Sardinella longiceps) and Indian mackerel ( Rastrelliger kanagurta) at four different temperatures, namely 36 ± 1°C, 28 ± 2°C (RT), 8 ± 1°C and 1 ±1°C, are reported. The total plate count at RT of the skin of oil sardine and Indian mackerel were in the range of l0 super(3) to 10 super(7) and 10 super(4) to 10 super(6) per cm², that of gills in the range of 10 super(5) to 10 super(9) and 10 super(4) to 10 super(8) per g and that intestine in the range of 10 super(5) to 10 sueper(9) and 10 super(5) to 10 super(8) per g respectively. The TPCs were markedly affected by the incubation temperature. Incubation at 28 ± 2°C gave the highest count; at 36 ± 1°C and 8 ± 1°C, the counts decreased by nearly 1-2 log cycles from that at RT. Incubation at 1 ± 1°C registered the lowest count. The peak values for bacterial counts of these fishes occurred at different periods of the year.

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Assessment of quality of fish and shrimps landed at the fish harbour, Cochin, was made over the period January, 1980 to December, 1982. A total of 201 samples were analysed. Nearly 75% of the samples scored between 6-10 in a 10 point hedonic scale and the percentage unacceptability based on sensory assessment was 5. 5%. In 10.1% of the samples, total volatile nitrogen was >30 mg% and in 8.3% of the samples trimethylamine- nitrogen was >10 mg%. Both, the gr Torrymeter and Intellectron Fish Tester VI readings marked significant correlation with sensory scores and chemical indices; but failed to bear any significant correlation with bacterial counts. Of the 5-1 total samples 66.7% had total plate count (TPC) ≥10 super(5) g super(-1) and 8.5% were considered unacceptable based on TPC >5x10 super(5) g super(-1); 63.2% of the samples were free from Escherichia coli; 26.4% had >20 E. coli g super(-1) and 20.4% of the samples contained faecal streptococci >10 super(3) g super(-1). Seven percent of the samples showed coagulase positive staphylococci >100 g super(-1). Salmonella could not be detected in any of the samples examined. Crushed ice samples and the platform of the harbour had high bacterial loads.

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Bacteria in the seawater used in P. monodon hatchery operations were isolated on Bachmann's agar. The total plate counts in 25 isolations ranged from 1.0 - 5.0 x 102 to 5.1 -10.0 x 105 cells per ml. Out of 124 isolates, 98 (79 percent) were Gram-positive and 26 (21 percent) were Gram-negative. Micrococcus and Staphylococcus were dominant in the former group, while Acinetobacter, Moraxella, Flavobacterium and Alcaligenes were most numerous in the latter. Twenty-nine of the Gram-positive isolates closely resembled Peptostreptococcus, Planococcus, and Pediococcus.

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咖啡旋皮天牛、咖啡灭字虎天牛均为云南省思茅热区咖啡树的重要蛀干害虫之一。通过对该2 种害虫各虫态形 态特征、发生危害规律、生物生态学习性、幼虫取食危害特性系统比较研究,综合二者发生代次、时空、危害特性异同, 抓住主要代次和关键时机开展综合防治,连续3 年在云南思茅热区联合开发办公室所属的茶树林、曼歇坝2 咖啡场有 效控制了该2 种虫的发生危害,其危害株率分别由原来的10 %~15 % ,5 %~10. 5 %降为现在1. 2 ‰~2. 1 ‰,1. 5 ‰~ 3. 3 ‰。

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下载PDF阅读器2004年10月-2005年5月,在云南纳帕海自然保护区采用定点扫描法对越冬黑颈鹤(Grus nigricollis)的集群类犁和集群大小进行了观察.结果表明黑颈鹤夜间集群夜栖,形成较大的夜栖群,平均群体大小为67.9只(16-157,n=17):按照有无灰鹤加入,又将其分为同种集群和混种集群两种类型,其中同种集群的黑颈鹤数量占整个越冬种群的65.3%.在白昼,黑颈鹤以家庭鹤、集群鹤及特殊群体3种类型活动,家庭鹤和集群鹤的平均大小分别为2.7只(2-4,n=145)和16.1只(3-65,n=1017).黑颈鹤的集群大小并不稳定,在日内和月份间均有明显变化(P=0.000<0.05).存越冬期,最大集群形成于12月,其次为11月和1月;在日内,早上8时集群最大,随后减小并保持相对稳定,18时黑颈鹤开始向夜栖地靠拢,使得集群再次开始增大.随后观察中还发现,黑颈鹤的家庭解体过程开始于3月底,当幼鹤被成鹤驱逐离群后,逐渐加入集群鹤活动,从而使得家庭鹤和集群鹤的大小和组成发生改变.黑颈鹤的集群大小和组成受自身状况、种内关系、天气、食物等多种因素的共同影响,随时间和季节变动而发生变化,是对自身、种群和环境条件变化的综合反映.

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用角膜表皮刮磨方法人工造成兔眼角膜创伤直径为10mm的圆形创面,分析小鼠表皮生长因子(EGF)对其修复的剂量关系。5个浓度EGF(100、50、20、10和0.5μm/ml)治疗的试验组痊愈时间均比对照组提前,有非常显著的差异(P<0.01),但在这5个浓度组之间,痊愈时间无显著性差异。结果表明,小鼠EGF对兔眼角膜损伤有促修复作用,实验所用的5个浓度与修复作用无剂量依赖关系。

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从超数排卵的14只母兔获得438枚受精卵, 卵龄16-22h。显微操作在带微分干涉和相差的Nikon倒置显微镜下进行。注射针的尖端外径0.5#mu#m, 离尖端40和80#mu#m处的外径分别为4.2和6.5#mu#m。注射用外源基因是绵羊生长激素基因与MT-1启动基因藕连的线状DNA溶液(1ng/#mu#l)。140枚注射的受精卵和未注射的145枚受精卵(对照), 在Ham^sF-10培养液(补充生长因子)中培养(38℃, 5%的CO_(2))。结果培养48h后, 注射组卵裂发育率分别是: 未卵裂7.9%(11/140)、卵裂至2-4细胞期11.0%(16/140)、卵裂至8-16细胞期80.7%(113/140)。对照组相应的卵裂率分别是4.1%(6/145)、12.4%(18/145)和83.4%(121/145)。两组卵裂发育率相近。

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2006年10月2007年5月,在云南省西北部纳帕海,采用路线调查结合瞬时扫描行为取样法,对越冬黑颈鹤(Grus nigricollis)种群的时间分配及其与年龄、集群和时间的关系进行了观察.结果说明,黑颈鹤越冬活动主要足以觅食为主,占日间时间的(76.81±9.1)%.越冬期间黑颈鹤日间行为的节律性较为明显,具有适应高寒气候的特点.集群形式对成鹤的行为有着显著影响,集群和家庭中活动的成鹤红觅食、警戒和争斗中存在显著差异(F1.76=0.27、0.77,U=2779,P=0.001-0.000).年龄是影响鹤群行为的因素之一.幼鹤相比成鹤有较多的觅食时间和休息时间,警戒行为比例较低(F1.76=0.04-2.59,U=188-2779,P=0.006-0.000),且小受集群形式的影响.随着越冬期间的早、中、晚3个时期的环境变化,黑颁鹤的时间分配有显著变化(F2.36=4.63-26.54,x22=5.29-13.68,P=0.0016-0.000).不同越冬地的黑颈鹤行为存在差异.气候、食物资源和人为影响可能是造成这些差异的主要因素.

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Increased stocks of mukene Rastrineobola argentea and the subsequent interest in its fishery on Lake Victoria has been attributed to the poor performance of the endemic fishery as a result of introductions into this lake of foreign fish species Lates niloticus and Oreochromis niloticus. R. argentea now remains the only endemic fish species of economic importance ranking second to the Nile perch in this lake. Despite this importance, biological information on the species and knowledge of its fishery is scanty. Preliminary observations on the species in the Ugandan waters indicate that R. argentea feeds mainly on zooplankton (copepods) during daylight hours. Small quantities of aquatic insect larvae/pupae (chironomids and chaoborids) are also eaten mainly at night. These fishes breed just after the rainy seasons and the young eventually mature at between 43-44 mm standard length. Growth and population parameters show a rate of growth (K) of 0.92 with L of 64.5 mm S.L. Natural mortality (M) is given at 2.371 and total mortality (2) of 3.594. Two mesh size nets 10 and 5 mm are in use in the lake. The smaller mesh size which is more preferred by the artisanal fishermen however tends to capture many immature fishes. There is therefore need for a unified lakewide data collection on the species and its fishery in order to obtain more reliable biological information necessary proper management of this fast developing fishery.

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对滇南和滇西茶叶害虫群落结构与生态分布进行了研究。该区域的茶叶害 虫隶属9 目, 57 科, 103 属, 167 种; 其中, 鳞翅目有16 科, 27 属, 53 种; 同翅目 13 科, 25 属, 35 种; 鞘翅目12 科, 23 属, 37 种; 直翅目4 科, 8 属, 17 种; 半翅 目4 科, 7 属, 10 种; 等翅目3 科, 5 属, 5 种; 缨翅目2 科, 4 属, 5 种; 竹节虫 目2 科, 2 属, 2 种; 双翅目1 科, 2 属, 3 种。同翅目、鳞翅目和鞘翅目的科、属、 种是整个茶园中优势类群; 直翅目、半翅目, 等翅目和缨翅目的科、属、种是茶叶 中常见类群; 竹节虫目和双翅目的科、属 种稀少。滇南和滇西的茶叶害虫绝大部 分属于东洋区成分种; 其生态类型可分为: 食嫩芽类型、食叶类型, 食果实类型, 为 害枝杆类型和食根及幼苗类型。

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This paper focuses on simplifying and easing the integration of a new machine into an existing conventional hierarchical manufacturing system. Based on a distributed manufacturing paradigm, it proposes the functions and interfaces that a new machine and an existing manufacturing system should possess so that ready and simple configuration of additional machines can be achieved. The configuration process is intended to include, not only mechanical and electrical interfaces, but also decision system interfaces (such as planning, scheduling, and shop-floor control). Preliminary laboratory experiments to compare the reconfigurability resulting from a conventional integration method and the proposed distributed method are presented and discussed. © 2007 ISAM.