A novel dual-fluorescence strategy for functionally validating microRNA targets in 3-prime untranslated regions: regulation of the inward rectifier potassium channel Kir2.1 by miR-212.

Gene targeting by microRNAs is important in health and disease. We developed a functional assay for identifying microRNA targets and applied it to the K+ channel Kir2.1 (KCNJ2) which is dysregulated in cardiac and vascular disorders. The 3'UTR was inserted downstream of the mCherry red fluorescent protein coding sequence in a mammalian expression plasmid. MicroRNA sequences were inserted into the pSM30 expression vector which provides enhanced green fluorescent protein as an indicator of microRNA expression. HEK293 cells were co-transfected with the mCherry-3'UTR plasmid and a pSM30-based plasmid with a microRNA insert. The principle of the assay is that functional targeting of the 3'UTR by the microRNA results in a decrease in the red/green fluorescence intensity ratio as determined by automated image analysis. The method was validated with miR-1, a known downregulator of Kir2.1 expression, and was used to investigate targeting of the Kir2.1 3'UTR by miR-212. Red/green ratio was lower in miR-212-expressing cells compared to non-targeting controls, an effect that was attenuated by mutating the predicted target site. MiR-212 also reduced inward rectifier current and Kir2.1 protein in HeLa cells. This novel assay has several advantages over traditional luciferase-based assays including larger sample size, amenability to time course studies and adaptability to high-throughput screening.

The Politics of Minister Retention in Presidential Systems: Technocrats, Partisans, and Government Approval

This article examines the impact of presidential approval and individual minister profiles on minister turnover. It claims that, in order to prioritize sustainable policy performance and cabinet loyalty, government chiefs protect and remove technocrats, partisans, and outsider ministers conditional on government approval. The study offers an operational definition of minister profiles that relies on fuzzy-set measures of technical expertise and political affiliation, and tests the hypotheses using survival analysis with an original dataset for the Argentine case (1983–2011). The findings show that popular presidents are likely to protect experts more than partisan ministers, but not outsiders.

Reflections upon the late correspondence between Mr. Secretary Smith, and Francis James Jackson, esq., Minister Plenipotentiary of His Britannic Majesty /

Originally published in the Federal Republican of Baltimore.

Reflections upon the late correspondence between Mr. Secretary Smith, and Francis James Jackson, esq., Minister Plenipotentiary of His Britannic Majesty

Originally published in the Federal Republican of Baltimore. Printed by Benjamin Edes

Letter - William Lyon Mackenzie King (Deputy Minister of Labour) to The Niagara Power Company, 5 October 1903

A letter from William Lyon Mackenzie King to The Niagara Power Company in the year 1903. Mackenzie King is Deputy Minister of Labour for the Department of Labour Canada and in this letter he discusses issues with importing of men from the United States to Canada for employment. The letter warns of penalty if found guilty of unlawfully importing men for employment.

A Memoir of Mary Capper, Late of Birmingham, a Minister of the Society of Friends.

UANL

REIDEMEISTER SPECTRUM FOR METABELIAN GROUPS OF THE FORM Q(n) x Z AND Z[1/p](n) x Z, p PRIME

In this paper, we study the Reidemeister spectrum for metabelian groups of the form Q(n) x Z and Z[1/p](n) x Z. Particular attention is given to the R(infinity)-property of a subfamily of these groups.

The corporate financial effects of corporate social responsibility, as executed by, and impacted upon, Prime Bank Limited, Bangladesh

Corporate Social Responsibility (CSR) is being implemented in the corporate world at an ever increasing rate, benefitting societies around the world. Several theories have been proposed that contend that the corporations who are implementing CSR programs also benefit financially, making the relationship a symbiotic one. This paper analyzes the financial health of Prime Bank Limited, Bangladesh, (PBL) over a period of a decade in order to determine if PBL has indeed benefited financially from implementing its CSR program. The analysis focuses on examining PBL’s internal and external financial indicators over an extended period of time to determine what the net effect, if any, that the CSR program has had on them. This analysis concludes that the evidence does not support the claim of a causal relationship between CSR spending and positive effects upon PBL, as measured by PBL’s financial indicators.

Neuroscience and education: prime time to build the bridge

As neuroscience gains social traction and entices media attention, the notion that education has much to benefit from brain research becomes increasingly popular. However, it has been argued that the fundamental bridge toward education is cognitive psychology, not neuroscience. We discuss four specific cases in which neuroscience synergizes with other disciplines to serve education, ranging from very general physiological aspects of human learning such as nutrition, exercise and sleep, to brain architectures that shape the way we acquire language and reading, and neuroscience tools that increasingly allow the early detection of cognitive deficits, especially in preverbal infants. Neuroscience methods, tools and theoretical frameworks have broadened our understanding of the mind in a way that is highly relevant to educational practice. Although the bridge’s cement is still fresh, we argue why it is prime time to march over it.