Endophytic population of Pantoea agglomerans in citrus plants and development of a cloning vector for endophytes

Harmless bacteria inhabiting inner plant tissues are termed endophytes. Population fluctuations in the endophytic bacterium Pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. The results demonstrated that populations of P. agglomerans fluctuated in Citrus reticulata but not C. sinensis. A cryptic plasmid pPA3.0 (2.9 kb) was identified in 35 out of 44 endophytic isolates of P. agglomerans and was subsequently sequenced. The origins of replication were identified and nine out of 18 open reading frames (ORFs) revealed homology with described proteins. Notably, two ORFs were related to cellular transport systems and plasmid maintenance. Plasmid pPA3.0 was cloned and the gfp gene inserted to generate the pPAGFP vector. The vector was introduced into P. agglomerans isolates and revealed stability was dependent on the isolate genotype, ninety-percent stability values were reached after 60 hours of bacterial cultivation in most evaluated isolates. In order to definitively establish P. agglomerans as an endophyte, the non-transformed bacterium was reintroduced into in vitro cultivated seedlings and the density of inner tissue colonization in inoculated plants was estimated by bacterium re-isolation, while the tissue niches preferred by the bacterium were investigated by scanning electronic microscopy (SEM). Cells from P. agglomerans (strain ARB18) at similar densities were re-isolated from roots, stems and leaves and colonization of parenchyma and xylem tissues were observed. Data suggested that P. agglomerans is a ubiquitous citrus endophyte harboring cryptic plasmids. These characteristics suggest the potential to use the bacterium as a vehicle to introduce new genes in host plants via endophytic bacterial transformation.

Novel sequence types (STs) of Staphylococcus aureus isolates causing clinical and subclinical mastitis in flocks of sheep in the northeast of Brazil

Staphylococcus aureus is one of the most important infectious mastitis causative agents in small ruminants. In order to know the distribution of Staph. aureus strains associated with infectious mastitis in flocks of sheep in the northeast of Brazil and establish whether these clones are related to the strains distributed internationally, this study analysed the genetic diversity of Staph. aureus isolates from cases of clinical and subclinical mastitis in ewes by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this research, 135 ewes with mastitis from 31 sheep flocks distributed in 15 districts were examined. Staph. aureus was isolated from sheep milk in 9 (29%) out of 31 herds located in 47% of the districts surveyed. MLST analysis allowed the identification of four STs (ST750, ST1728, ST1729 and ST1730). The last three with their respective novel alleles (g/p-220; pta-182 and yqil-180) were recently reported in the Staph. aureus MLST database (http://www.mlst.net). Each novel allele showed only a nucleotide different from those already described. The occurrence of CC133 (ST750 and ST1729) in this study is in agreement with other reports that only a few clones of Staph. aureus seem to be responsible for most cases of mastitis in dairy farms and that some of these clones may have broad geographic distribution. However, the prevalence of CC5 (ST1728 and ST1730)-an important group related to cases of colonization or infection in humans-differs from previous studies by its widespread occurrence and may suggest human contamination followed by selective pressures of the allelic diversifications presented for these STs.

The use of transcriptional profiles to predict adult mosquito age under field conditions

Age is a critical determinant of an adult female mosquito's ability to transmit a range of human pathogens. Despite its central importance, relatively few methods exist with which to accurately determine chronological age of field-caught mosquitoes. This fact is a major constraint on our ability to fully understand the relative importance of vector longevity to disease transmission in different ecological contexts. It also limits our ability to evaluate novel disease control strategies that specifically target mosquito longevity. We report the development of a transcriptional profiling approach to determine age of adult female Aedes aegypti under field conditions. We demonstrate that this approach surpasses current cuticular hydrocarbon methods for both accuracy of predicted age as well as the upper limits at which age can be reliably predicted. The method is based on genes that display age-dependent expression in a range of dipteran insects and, as such, is likely to be broadly applicable to other disease vectors.

Reliability and validity of physical fitness field tests for adults aged 55 to 70 years

The aim of this study was to examine the reliability and validity of field tests for assessing physical function in mid-aged and young-old people (55–70 y). Tests were selected that required minimal space and equipment and could be implemented in multiple field settings such as a general practitioner's office. Nineteen participants completed 2 field and 1 laboratory testing sessions. Intra-class correlations showed good reliability for the tests of upper body strength (lift and reach, R= .66), lower body strength (sit to stand, R= .80) and functional capacity (Canadian Step Test, R= .92), but not for leg power (single timed chair rise, R= .28). There was also good reliability for the balance test during 3 stances: parallel (94.7% agreement), semi-tandem (73.7%), and tandem (52.6%). Comparison of field test results with objective laboratory measures found good validity for the sit to stand (cf 1RM leg press, Pearson r= .68, p< .05), and for the step test (cf PWC140, r= −.60, p< .001), but not for the lift and reach (cf 1RM bench press, r= .43, p> .05), balance (r= −.13, −.18, .23) and rate of force development tests (r= −.28). It was concluded that the lower body strength and cardiovascular function tests were appropriate for use in field settings with mid-aged and young-old adults.

Field prevalence of Wolbachia in the mosquito vector Aedes albopictus

The endosymbiotic bacteria in the genus Wolbachia have been proposed as a potential candidate to deliver pathogen-blocking genes into natural populations of medically important insects. The successful application of Wolbachia in insect vector control depends on the ability of the agent to successfully invade and maintain itself at high frequency under field conditions. Here, we evaluated the prevalence of Wolbachia infections in a field population of the Wolbachia-superinfected mosquito Aedes albopictus. A field prevalence of 100% (n = 1,016) was found in a single population in eastern Thailand via polymerase chain reaction (PCR) testing of Wolbachia both from individual parent females and their corresponding F1 offspring. This is the first report of accurate Wolbachia prevalence in a field population of an insect disease vector. The prevalence of superinfection was estimated to be 99.41%. All single-infected individual mosquitoes (n = 6) were found to harbor group A Wolbachia. For this particular population, none was found to be single-infected with group B Wolbachia. Our results also show that PCR testing of field materials alone without checking F1 offspring overestimated the natural prevalence of single infection. Thus, the confirmation of infection status by means of F1 offspring was critical to the accurate estimates of Wolbachia prevalence under field conditions.

The fornax spectroscopic survey: the number of unresolved compact galaxies

We describe a sample of 13 bright (18.5 < B-J < 20.1), compact galaxies at low redshift (0.05 < z < 0.21) behind the Fornax Cluster. These galaxies are unresolved on UK Schmidt sky survey plates, and so they would be missing from most galaxy catalogs compiled from this material. The objects were found during initial observations of The Fornax Spectroscopic Survey. This project is using the Two-degree Field spectrograph on the Anglo-Australian Telescope to obtain spectra for a complete sample of all 14,000 objects, stellar and nonstellar, with 16.5 < B-J < 19.7, in a 12 deg(2) area centered on the Fornax Cluster of galaxies. The surface density of compact galaxies with magnitudes 16.5 < B-J < 19.7 is 7 +/- 3 deg(-2), representing 2.8% +/- 1.6% of all local (z < 0.2) galaxies to this limit. There are 12 +/- 3 deg(-2) with 16.5 < B-J < 20.2. They are luminous (-21.5 < M-B < -18.0, for H-o = 50 km s(-1) Mpc(-1)), and most have strong emission lines (H alpha equivalent widths of 40-200 Angstrom) and small sizes typical of luminous H II galaxies and compact narrow emission line galaxies. Four out of 13 have red colors and early-type spectra, and so they are unlikely to have been detected in any previous surveys.

Validation of non-fluorescent methods to reliably detect acrosomal and plasma membrane integrity of common marmoset (Callithrix jacchus) sperm

Simple, rapid and stable sperm evaluation methods which have been optimized for common marmoset (Callithrix jacchus) are critical for studies involving collection and evaluation of sperm in the field. This is particularly important for new species groups such as Callitrichidae where the sperm have been little studied. Of this family, C jacchus is the best known, and has been chosen as a model species for other members of the genus Callithrix. The fundamental evaluation parameters for sperm of any species are viability and acrosomal status. Semen samples were collected by penile vibratory stimulation. To evaluate sperm plasma membrane integrity, Eosin-Nigrosin was tested here for the common marmoset sperm to be used under field conditions. Further, a non-fluorescent stain for acrosome, the ""Simple"" stain, developed for domestic and wild cats, was tested on common marmoset sperm. This was compared with a fluorescent staining, Fluorescein isothiocyanate-Pisum sativum agglutinin (FITC-PSA), routinely used and validated for common marmoset at the German Primate Centre to evaluate acrosomal integrity. Results obtained with the ""Simple"" stain showed a marked differentiation between sperm with intact and non-intact acrosome both with and without ionophore treatment and closely correlated with results obtained with FITC-PSA. Temperature had no effect on the results with the ""Simple"" stain and the complete processing is simple enough to be carried out under field conditions. These findings indicated that the ""Simple"" stain and Eosin-Nigrosin provide rapid and accurate results for C. jacchus sperm and that those methods can be reliably used as field tools for sperm evaluation for this species. (c) 2008 Elsevier Inc. All rights reserved.

Effect of body size on fecundity of Childers canegrub, Antitrogus parvulus Britton (Coleoptera : Scarabaeidae)

Actual and potential fecundity for Childers canegrub, Antitrogus parvulus Britton, was influenced by the size of females, with the largest females laying the most eggs. Actual or realised fecundity for A. parvulus averaged 18 eggs per female, about half of potential fecundity. Actual fecundity was significantly related to elytron length in a group of laboratory-reared beetles, but not for a group of field-collected beetles. Size was related to potential fecundity for four out of four groups of females collected from emergence traps in the field and for one of two groups reared in the laboratory from field-collected late-instar larvae. As females lay a single batch of eggs, beetle size may be important in the population dynamics of A. parvulus. Populations of A. parvulus with small beetles are potentially less likely to persist and expand than populations with relatively large females.

Factors affecting the establishment and dispersal of nymphs of Pristhesancus plagipennis Walker (Hemiptera : Reduviidae) when released onto soybean, cotton and sunflower crops

A problem with augmenting predatory bugs through mass release is the logistical difficulty of delivering nymphs onto the foliage of field crops. In this paper we examine postrelease establishment and dispersal of the nymphs of the predatory bug Pristhesancus plagipennis on soybean, cotton and sunflower in an effort to devise an appropriate strategy for field release. The effects of predator stadia and release rates on field establishment and within-crop-canopy dispersal after hand release were recorded in soybean, cotton and sunflower. Field establishment improved with the release of more-developed nymphs, with third instars providing the most appropriate compromise between field hardiness and rearing cost. Increased nymphal density at the point of release had little effect on nymphal dispersal throughout the crop canopy. The patterns of nymphal dispersal observed on the three crops suggest that crop-canopy architecture may have an effect on the ability of nymphs to spread out postrelease, as nymphs dispersed poorly in cotton and sunflower compared to soybean. To overcome poor dispersal of nymphs after release, a mechanical release method, where nymphs were mixed with vermiculite and delivered onto a target crop through a spinning disk fertiliser spreader, was tested, and provided similar nymph establishment rates and dispersal patterns as releasing nymphs individually by hand. The implications of nymph dispersal and field hardiness in regard to inundative field release techniques are discussed.

Are wing size, wing shape and asymmetry related to field fitness of Trichogramma egg parasitoids?

The effects of wing shape, wing size, and fluctuating asymmetry in these measures oil the field fitness of T. nr. brassicae and T. pretiosum were investigated. Trichogramma wasps mass-reared on eggs of the factitious host Sitotroga cerealella were released in tomato paddocks and those females ovipositing on Helicoverpo spp. eggs were recaptured. Comparisons of the recaptured group with a sample from the release population were used to assess fitness. Wing data were obtained by positioning landmarks on mounted forewings. Size was then measured as the centroid size computed from landmark distances, while Procrustes analysis followed by principal component analysis was used to assess wing shape. Similar findings were obtained for both Trichogramma species: fitness of wasps was strongly related to wing size and some shape dimensions, but not to the asymmetries of these measures. Wasps which performed well in the field had larger wings and a different wing shape compared to wasps from the mass reared population. Both size and the shape dimensions were linearly associated with fitness although there was also some evidence for non-linear selection on shape. The results suggest that wing shape and wing size are reliable predictors of field fitness for these Trichogramma wasps.

A criança com Síndrome de Asperger na educação infantil : um estudo de caso

Esta pesquisa teve como objetivo compreender as ações pedagógicas constituídas por uma unidade municipal de ensino de Vitória/ES, visando ao processo de inclusão escolar de uma criança com Síndrome de Asperger no contexto da Educação Infantil. Contou com as contribuições teóricas dos estudos da matriz histórico-cultural e de autores dedicados a estudar a infância, como Kramer, Sarmento, Aries, dentre outros, bem como de pesquisadores interessados em investigar os pressupostos da Educação Especial em uma perspectiva inclusiva. Como aporte teórico-metodológico, apoiou-se no estudo de caso do tipo etnográfico que advoga pela possibilidade de, por meio da pesquisa científica, produzir conhecimento sobre a realidade social. O trabalho de pesquisa foi realizado em uma unidade municipal de Educação Infantil de Vitória/ES, envolvendo uma criança com Síndrome de Asperger, professores, pedagogos, dirigente escolar e responsável pelo estudante investigado. O processo de coleta de dados se efetivou no período de março de 2013 a setembro de 2013. O pesquisador esteve de uma a duas vezes por semana no campo de pesquisa, participando das observações em sala de aula, em espaços para planejamento e formação continuada e também observando momentos informais na entrada, recreio e saída dos alunos. Para a organização do estudo, trabalhou com quatro eixos: a) ações implementadas em favor do processo de inclusão escolar de alunos com Síndrome de Asperger no contexto da Educação Infantil; b) proposta pedagógica do CMEI “Alegria da Cinderela”: espaços de planejamento, formação e utilização dos apoios pedagógicos para a inclusão escolar; c) concepções dos profissionais envolvidos na pesquisa e da família sobre a inclusão escolar da criança com Síndrome de Asperger; d) principais possibilidades e/ou dificuldades encontradas pela unidade de ensino mediante o processo de ensino-aprendizagem da criança com Síndrome de Asperger. Como resultados, a pesquisa aponta: a importância de pensar nessas crianças como sujeitos de direitos e capazes de aprender; a necessidade de investimentos na formação inicial e continuada de professores para que os estudantes tenham maiores possibilidades de aprender; a urgência de o professor assumir a inclusão escolar como um movimento ético comprometido com a formação e com o reconhecimento da diversidade/diferença humana; a necessidade de reconhecer o cotidiano da Educação Infantil como um rico espaço para todas as crianças se desenvolverem e produzirem conhecimentos com seus pares e por meio das mediações pedagógicas dos professores.

Estresse e desordem temporomandibular (DTM) : incidência e relação em docentes de nível

RESUMO: O trabalho docente exige dedicação em sala de aula e fora dela, pois o professor vê-se na missão de educador, formador de opiniões e de futuros profissionais no mercado de trabalho. Uma das patologias que pode ocorrer devido ao estresse gerado pela docência é o desenvolvimento de Desordens Temporomandibulares, que acarretará cefaleias, dores musculares na região cervical e facial e alterações posturais. Tivemos a pretensão de conduzir nossas reflexões sobre o estresse do docente para procurar responder às nossas inquietações. Para isso, formulamos o seguinte questionamento: qual a incidência e a relação entre o estresse e o desenvolvimento de Desordens Temporomandibulares no docente de nível superior? O objetivo geral desta investigação foi descrever e analisar a relação entre o estresse e a incidência de Desordem Temporomandibular (DTM) em docentes de nível superior da cidade de Palmas – Tocantins – Brasil. Para contemplar o objetivo geral, buscamos verificar a presença ou não de estresse e os principais sintomas de estresse nos docentes de Ensino Superior, identificando a fase do estresse em que os professores se encontravam e verificar a presença ou não de DTM e seus graus. Procuramos, ainda, avaliar a relação entre sexo e presença de estresse, estado civil e presença de estresse, o tempo de docência e a presença de estresse e a relação entre a carga horária em sala de aula e a carga horária total de trabalho e a presença de estresse, entre sexo e presença de DTM, estado civil e presença de DTM, o tempo de docência e a presença de DTM e a relação entre a carga horária em sala de aula e carga horária total de trabalho e a presença de DTM. A metodologia utilizada, para a realização deste trabalho, foi de caráter exploratório e descritivo com uma abordagem quantitativa dos dados obtidos. A pesquisa caracterizou-se num estudo de campo, fundamentada em um instrumento denominado “Inventário de Sintomas de Stress Lipp – ISSL” da autora Marilda Lipp (2005), versão para adulto, ao qual acrescentamos dados sociodemográficos (estado civil, tempo de docência, carga horária de sala de aula e de trabalho total). Recorremos, ainda, ao Questionário Índice de Helkimo para identificar a ocorrência de DTMs. A coleta dos dados foi realizada a partir de uma amostra de duzentos e trinta e três (233) docentes de Ensino Superior, com idade entre 23 e 74 anos. Com base na análise realizada, os resultados revelaram que a presença de sintomas de estresse se encontra em 106 (45,49%) docentes, com predomínio da fase de resistência. Verificamos que 127 (54,5%) docentes não apresentam estresse. A sintomatologia predominante são sintomas psicológicos. Vimos que o tempo de docência e a jornada de trabalho não contribuem como um valor preditivo da presença de estresse, bem como o estado civil, e verificamos que o sexo feminino apresentou mais sintomas de estresse. Ao analisarmos a presença ou não de DTM, vimos que os docentes apresentam sintomas de DTM em sua maioria, encontrada em 187 docentes (80,25%), com predomínio do grau de DTM leve. Vimos que o tempo de docência e a jornada de trabalho não contribuem como um valor significativo para a presença de DTM, mas que o sexo feminino apresentou mais sintomas de DTM, bem como os casados. Buscando responder ao problema norteador desta 6 investigação, vimos que, mesmo nos docentes sem presença de estresse, havia sintomas de DTM. Esses dados indicam que a profissão docente pode causar sobrecargas e gerar a DTM. Diante dessa amostra de docentes pesquisados, concluímos que os dados aqui apresentados sugerem uma ampliação do estudo. Considerando a relevância do papel desempenhado pelos docentes das Instituições de Ensino Superior na formação dos acadêmicos e em sua efetiva transformação em futuros profissionais, nossa contribuição para o conhecimento do processo de estresse e os graus de DTM na atividade docente proporcionará oportunidade aos profissionais das diversas áreas do conhecimento e, principalmente, aos docentes para despertarem para a elaboração de programas de combate, controle e prevenção do estresse e do desenvolvimento de DTMs, resultando, dessa forma, na conquista de uma vida mais saudável, tanto na área física quanto na área psicológica. ABSTRACT: Abstract The teacher’s job requires dedication in and out of the classroom for a teacher is in a education mission, he/she is an opinion maker and responsible for future professionals in the market. One of the pathologies that may take place due to the stress caused by this job is the Temporomandibular Joint Disorder (TMD) that will trigger cephaleas, muscle pain in the cervical and facial area and posture changes. The purpose of this paper is to conduct reflections about teachers’ stress trying to answer the following question: What is the incidence rate and correlation between stress and the occurrence of Temporomandibular Joint Disorder (DTM) in teachers of institutions of higher learning in the city of Palmas – Tocantis – Brazil. To fulfill the general purpose we tried to verify the presence or not of stress and the principal symptoms in teachers in universities and colleges, identifying the levels of stress that they were, and verifying the presence or not of TMD and its levels. We tried to evaluate the correlation between gender and the occurrence of stress, marital status and the presence of stress, career time and the presence of stress, and time working in classroom and the total time of work and the presence of stress. The methodology used to perform this was an exploratory and descriptive study with a quantitative approach of the data gathered. The research was characterized by a field study, fundamented by a tool called “Lipp Stress Symptom Inventory – ISSL” from the author Marilda Lipp (2005), adult version, which we also added social-demographic data (marital status, time teaching, hours working in the classroom, and total time of work). We also used the Helkimo Index Questionnaire to identify the occurrence of TMD’s. The data was collected from a sample of two hundred three (233) teachers of universities and colleges between 23 to 74 years of age. Based on the analysis performed, the results showed the presence of stress among 106 (45.49%) of the teachers, with the predominance of the resistance level. We observed that some 127 teachers (54.5%) did not show stress. The predominant symptomology were psychological ones. We observed that time of experience and the schedule do not influence as a predictive value for the stress presence, neither does the marital status and we also observed that the female gender showed more stress symptoms. When we analyzed the presence or not of TMD, we observed that most of the teachers showed symptoms of TMD, some 187 teachers (80.25%), with the predominance of first level TMD. We observed that the time as teacher and the schedule do not influence with an important value for the TMD presence, however the feminine gender as well as married teachers showed more symptoms of TMD. Trying to answer the most important issue of this study, we saw that even without the occurrence of stress the TMD was present showing that the profession can cause overwork and consequently TMD. With this sample of teachers studied we conclude that the found data suggest a wider research. Considering the role performed by teachers of universities and colleges in the development of students and future professionals, our contribution for the understanding of the stress development and TMD levels in the teaching activity will enable the opportunity for professionals of different areas of knowledge, and 8 principally for teachers, to awaken to the need to develop programs to fight, control, and prevent stress and the development of TMD, therefore resulting in a healthier life, both a physical as well as a psychological one.

Temporal and spatial variability of fin whale's (Balaenoptera physalus) distribution in the Pelagos Sanctuary: Northwestern Mediterranean Sea

Dissertação de Mestrado, Estudos Integrados dos Oceanos, 20 de Março de 2014, Universidade dos Açores.

O pensamento algébrico e a descoberta de padrões na formação de professores

Este texto incide sobre o tema do pensamento algébrico nos primeiros anos de escolaridade ao nível da formação contínua de professores. Na abordagem apresentada o tema dos padrões surge como contexto estruturante para o desenvolvimento do pensamento algébrico. Começa-se por fazer um enquadramento dos referenciais teóricos que fundamentam a adoção de uma proposta didática desenvolvida nesse âmbito, recorrendo em seguida a um estudo empírico que foi desenvolvido, do qual se relata aqui uma parte centrada nas práticas de sala de aula de uma professora do primeiro ciclo em formação, de modo a poder confirmar a eficácia da proposta no ensino e desenvolvimento do pensamento algébrico dos alunos. Os resultados permitem concluir que a professora interiorizou e projetou na sua prática os aspetos essenciais que conduziram ao desenvolvimento do pensamento algébrico nos alunos. O texto termina com algumas conclusões e implicações para a formação de professores.

Application of wetland systems in the treatment of acid rock mine drainage

The presented work was conducted within the Dissertation / Internship, branch of Environmental Protection Technology, associated to the Master thesis in Chemical Engineering by the Instituto Superior de Engenharia do Porto and it was developed in the Aquatest a.s, headquartered in Prague, in Czech Republic. The ore mining exploitation in the Czech Republic began in the thirteenth century, and has been extended until the twentieth century, being now evident the consequences of the intensive extraction which includes contamination of soil and sub-soil by high concentrations of heavy metals. The mountain region of Zlaté Hory was chosen for the implementation of the remediation project, which consisted in the construction of three cells (tanks), the first to raise the pH, the second for the sedimentation of the formed precipitates and a third to increase the process efficiency in order to reduce high concentrations of metals, with special emphasis on iron, manganese and sulfates. This project was initiated in 2005, being pioneer in this country and is still ongoing due to the complex chemical and biological phenomenon’s inherent to the system. At the site where the project was implemented, there is a natural lagoon, thereby enabling a comparative study of the two systems (natural and artificial) regarding the efficiency of both in the reduction/ removal of the referred pollutants. The study aimed to assist and cooperate in the ongoing investigation at the company Aquatest, in terms of field work conducted in Zlaté Hory and in terms of research methodologies used in it. Thereby, it was carried out a survey and analysis of available data from 2005 to 2008, being complemented by the treatment of new data from 2009 to 2010. Moreover, a theoretical study of the chemical and biological processes that occurs in both systems was performed. Regarding the field work, an active participation in the collection and in situ sample analyzing of water and soil from the natural pond has been attained, with the supervision of Engineer, Irena Šupiková. Laboratory analysis of water and soil were carried out by laboratory technicians. It was found that the natural lagoon is more efficient in reducing iron and manganese, being obtained removal percentages of 100%. The artificial lagoon had a removal percentage of 90% and 33% for iron and manganese respectively. Despite the minor efficiency of the constructed wetland, it must be pointed out that this system was designed for the treatment and consequent reduction of iron. In this context, it can conclude that the main goal has been achieved. In the case of sulphates, the removal optimization is yet a goal to be achieved not only in the Czech Republic but also in other places where this type of contamination persists. In fact, in the natural lagoon and in the constructed wetland, removal efficiencies of 45% and 7% were obtained respectively. It has been speculated that the water at the entrance of both systems has different sources. The analysis of the collected data shows at the entrance of the natural pond, a concentration of 4.6 mg/L of total iron, 14.6 mg/L of manganese and 951 mg/L of sulphates. In the artificial pond, the concentrations are 27.7 mg/L, 8.1 mg/L and 382 mg/L respectively for iron, manganese and sulphates. During 2010 the investigation has been expanded. The study of soil samples has started in order to observe and evaluate the contribution of bacteria in the removal of heavy metals being in its early phase. Summarizing, this technology has revealed to be an interesting solution, since in addition to substantially reduce the mentioned contaminants, mostly iron, it combines the low cost of implementation with an reduced maintenance, and it can also be installed in recreation parks, providing habitats for plants and birds.