N-glycosylation in sugarcane

The N-linked glycosylation of secretory and membrane proteins is the most complex posttranslational modification known to occur in eukaryotic cells. It has been shown to play critical roles in modulating protein function. Although this important biological process has been extensively studied in mammals, much less is known about this biosynthetic pathway in plants. The enzymes involved in plant N-glycan biosynthesis and processing are still not well defined and the mechanism of their genetic regulation is almost completely unknown. In this paper we describe our first attempt to understand the N-linked glycosylation mechanism in a plant species by using the data generated by the Sugarcane Expressed Sequence Tag (SUCEST) project. The SUCEST database was mined for sugarcane gene products potentially involved in the N-glycosylation pathway. This approach has led to the identification and functional assignment of 90 expressed sequence tag (EST) clusters sharing significant sequence similarity with the enzymes involved in N-glycan biosynthesis and processing. The ESTs identified were also analyzed to establish their relative abundance.

Comparação de métodos de determinação da gravidade específica de ovos de poedeiras comerciais

The objective of this work was to compare the results of measuring the specific gravity (SG) of laying hen eggs by using a weighing apparatus and the saline flotation method (SFM). Two hundred eggs from two genetic lines were distributed randomly into two groups of 50 eggs per genetic line and were submitted to two different sequences of SG measurement. Not significant differences were found between the two methods. The weighing apparatus measured the SG of the eggs accurately as the SFM.

In silico evaluation of the Eucalyptus transcriptome

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Facies sedimentares e ambientes deposicionais da Formação Barbalha no vale do cariri, bacia do araripe, nordeste do Brasil

The Aptian Barbalha Formation represents the first unit of the post-rift sequence of the Araripe Basin and crops out at the slopes of the Aranpe plateau in the eastern part ot Aranpe Basin. The unit has also been named Kio da Batateira Formation, but this name is here used in its original definition as Batateira Beds, an interval of great lateral continuity and characterized by the presence of bituminous shales of the Alagoas Stage (P-270 palynological biozone). This paper presents the results of a stratigraphic analysis carried out along the outcrop belt in order to establish the facies architecture and to interpret deposicional environments of the siliciclastic Barbalha Formation. Detailed stratigraphic vertical sections were measured and correlated. They allowed the recognition of two depositional sequences characterized by fining upward arrangement of facies, beginning with fluvial deposits and ending with lacustrine deposits at their tops. The end of the first cycle is represented by black shales and brecciated limestones of the Batateira Beds that record a geologic event of regional magnitude and serve as meaningful long-distance stratigraphic mark. The second deposicional sequence overlies disconformably the Batateira Beds and begins with clast-supported conglomerates, which are covered by a succession of fluvial sandstones and minor intervals of pelitic rocks. The sandstone content diminishes towards the top and the upper part of the unit is characterized by the presence of ostracode-rich green shales. The Barbalha Formation is conformably overlaid by Late Aptian lacustrine limestones belonging to the Crato Member of the Santana Formation.

Tuberculose e o estudo molecular da sua epidemiologia

Systems that can distinguish epidemiologically-related Mycobacterium tuberculosis strains from unrelated ones are extremely valuable. Molecular biology techniques have allowed a great deal of information to be acquired about the infectious disease tuberculosis (TB) that was very hard or impossible to obtain by conventional epidemiology. A typing method based on bacterial DNA genome differences, known as RFLP (restriction fragment length polymorphism), is widely used to discriminate strains in the epidemiologic study of TB. However, RFLP is laborious and there is a tendency to replace it by other methods. Thus, other DNA sequences have been employed as epidemiological markers, as in Spoligotyping, a fast technique based on PCR followed by differential hybridization of amplified products. The polymorphism observed among different isolates is probably the product of strain-dependent recombination. MIRU (mycobacterial interspersed repetitive unit) typing is a reproducible and fast assay, involving the generation of genotypes based on the study of 12 loci containing VNTRs (variable-number tandem repeats) in strains of the M. tuberculosis complex. It compares strains from different geographic areas and allows the movement of individual lineages to be tracked, as in RFLP. This approach enables a greater number of isolates to be analyzed, leading to the identification of a larger number of foci of transmission within the population and thus to improved ways of slowing the progress of the disease.

Screening of genomic libraries

Microsatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs. © Springer Science+Business Media, LLC 2013.

Assistência de ar e aplicação em volume baixo no controle da ferrugem asiática da soja (Phakopsora pachyrhizi Sydon & Sydon

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Construções de causa, razão, explicação e motivação na lusofonia: uma abordagem discursivo-funcional

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação das estruturas químicas derivadas de diaminas do ferroceno no metabolismo oxidativo em cepas de Trypanosoma cruzi

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Avaliação do perfil de expressão gênica de células CD34+ e células CD CD133+ isoladas de medula óssea e de sangue de cordão umbilical

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Toxicidade aguda e risco ambiental do diflubenzuron para Daphnia magna, Poecilia reticulata e Lemna minor na ausência e presença de sedimento

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

O ethos na linguagem da criança: um caminho para a identidade enunciativa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Proposta de atualização de cadastro urbano a partir de detecção de alterações em imagens QUICK BIRD tomadas em diferentes épocas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção de rodovias em imagens digitais de alta resolução com o uso da teoria de morfologia matemática

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização espectral e espacial de áreas infestadas por nematóides e Migdolus fryanusem em cultura canavieira

Pós-graduação em Ciências Cartográficas - FCT