Isolamento e resistência a antimicrobianos de cepas de Streptococcus spp. provenientes de rãs-touro (Lithobates catesbeianus)

Twelve bullfrogs were selected from two commercial frog farms and clinically diagnosed as attacked by Streptococcus disease. Sixty samples were collected, and Streptococcus spp. was isolated from all bullfrog, being 12 (100%) from the encephalus, seven from the kidneys (58.3%), three from the liver (25%), two from the spleen (16.6%), and one from the ascitic liquid (8.3%). Streptococcus -hemolytic were isolated from all the 60 samples, which were sensible to chloramphenicol (100%), gentamycin (100%), vancomycin (96%), cefotaxime (96%), and cefoxitine (92%).

Efeito do probiótico Bacillus subtilis no crescimento, sobrevivência e fisiologia de rãs-touro (Rana catesbeiana)

The probiotics are live microorganisms, in latency state, that benefices the development of the animals. The objective of this work was to evaluate the effect of the Bacillus subtilis in different doses about the growing, survival and immunological and hematological analyses in the Bullfrog (Rana catesbeiana) froglets. The test was developed in Experimental Frog farm of Agriculture Department, Pindamonhangaba, SP. Three doses of probiotics were tested (T1 - 2.5 g/Kg, T2 - 5 g/Kg and T3 - 10 g/Kg of food). The test was carried out with three simultaneous replicates, plus control group. The products were added to froglet's diet and mixed to the meal. The animals were previously feed with this diet for a period of 14 days and accompanied for about 42 days after metamorphose. Biometrics were performed every 7 days. It was evaluated the final weight, the survival, phagocytic capacity, phagocytic index and hematological analyses as hematocrit, hemoglobin level, erythrocyte number, absolute indices hematimetrycs (MCV and MCHC), differential counting of leucocytes and total counting of leucocytes and trombocytes. The results had shown that the doses of the probiotics had produced none effect on the final weight and survival. The immunological analyses had shown that the probiotics have presented immunostimulator effect, but haven't influenced the hematological parameters of the animals.

Influência da incorporação de vitamina C à dieta no desempenho produtivo de rãs-touro Lithobates catesbeianus pós-metamorfoseadas

The experiment was developed in Marta Farm, Tremembe city, located in the State of São Paulo - Brazil, using a frogculture commercial project. The aim of this study was evaluate the performance (growth, survival and feed conversion) of bullfrogs Lithobates catesbeianus, post-metamorphosed fed with diets containing different amounts of vitamin C and your performance with the temperature. The animals were kept in 24 boxes of (2.0 x 1.0 m) with an initial density of 50 animals per square meter. We tested the following levels of supplementation in the diet: 0, 250, 500, 750, 1,000 and 2,000 mg vitamin C kg(-1) of diet. The experimental design was completely randomized with six treatments and four replicates. The parameters evaluated were: feed intake, weight gain, feed conversion and survival tax. The animals were weighed at the beginning of the experiment and every 30 days and recorded daily to room temperature. The percentage of food intake decreased with the growth of the frogs, from 3.85 to 1.2% of the live weight, and the mean of temperature decreased from 28 to 20.1 degrees C. It was concluded that there was no influence of vitamin C on the performance (growth, survival and feed conversion) of bullfrogs in the growing stage.