Transient and d.c. analysis of the operation mechanism of light-emitting electrochemical cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Selectivity in the mechanism of action of antimicrobial mastoparan peptide Polybia-MP1

Many potent antimicrobial peptides also present hemolytic activity, an undesired collateral effect for the therapeutic application. Unlike other mastoparan peptides, Polybia-MP1 (IDWKKLLDAAKQIL), obtained from the venom of the social wasp Polybia paulista, is highly selective of bacterial cells. The study of its mechanism of action demonstrated that it permeates vesicles at a greater rate of leakage on the anionic over the zwitterionic, impaired by the presence of cholesterol or cardiolipin; its lytic activity is characterized by a threshold peptide to lipid molar ratio that depends on the phospholipid composition of the vesicles. At these particular threshold concentrations, the apparent average pore number is distinctive between anionic and zwitterionic vesicles, suggesting that pores are similarly formed depending on the ionic character of the bilayer. To prospect the molecular reasons for the strengthened selectivity in Polybia-MP1 and its absence in Mastoparan-X, MD simulations were carried out. Both peptides presented amphipathic alpha-helical structures, as previously observed in Circular Dichroism spectra, with important differences in the extension and stability of the helix; their backbone solvation analysis also indicate a different profile, suggesting that the selectivity of Polybia-MP1 is a consequence of the distribution of the charged and polar residues along the peptide helix, and on how the solvent molecules orient themselves according to these electrostatic interactions. We suggest that the lack of hemolytic activity of Polybia-MP1 is due to the presence and position of Asp residues that enable the equilibrium of electrostatic interactions and favor the preference for the more hydrophilic environment.

New Insight into the Mechanism of Action of Wasp Mastoparan Peptides: Lytic Activity and Clustering Observed with Giant Vesicles

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Study of the mechanism of action of anoplin, a helical antimicrobial decapeptide with ion channel-like activity, and the role of the amidated C-terminus

Anoplin, an antimicrobial, helical decapeptide from wasp venom, looses its biological activities by mere deamidation of its C-terminus. Secondary structure determination, by circular dichroism spectroscopy in amphipathic environments, and lytic activity in zwitterionic and anionic vesicles showed quite similar results for the amidated and the carboxylated forms of the peptide. The deamidation of the C-terminus introduced a negative charge at an all-positive charged peptide, causing a loss of amphipathicity, as indicated by molecular dynamics simulations in TFE/water mixtures and this subtle modification in a peptide's primary structure disturbed the interaction with bilayers and biological membranes. Although being poorly lytic, the amidated form, but not the carboxylated, presented ion channel-like activity on anionic bilayers with a well-defined conductance step; at approximately the same concentration it showed antimicrobial activity. The pores remain open at trans-negative potentials, preferentially conducting cations, and this situation is equivalent to the interaction of the peptide with bacterial membranes that also maintain a high negative potential inside. Copyright (C) 2007 European Peptide Society and John Wiley & Sons, Ltd.

Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crystallization and preliminary X-ray diffraction analysis of an l-amino-acid oxidase from Bothrops jararacussu venom

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Purification, crystallization and preliminary X-ray diffraction analysis of a class P-III metalloproteinase (BmMP-III) from the venom of Bothrops moojeni

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Crystallization, preliminary X-ray diffraction analysis and Patterson search of oxyhaemoglobin I from the wolf (Chrysocyon brachiurus)

Oxyhaemoglobin I isolated from the Brazilian wolf Chrysocyon brachiurus has been crystallized and X-ray diffraction data has been collected to 2.06 A resolution using a synchrotron-radiation source. Crystals were determined to belong to the space group P2(1)2(1)2(1) and preliminary structural analysis revealed the presence of one tetramer in the asymmetric unit. The structure was determined using standard molecular-replacement techniques and is currently being refined using maximum-likelihood protocols. This is the first haemoglobin isolated from a member of the Canidae family to be crystallized and it will provide further insights in the comparative biochemistry of vertebrate haemoglobins.

A dynamical mechanism to explain the top-bottom quark mass hierarchy

We discuss the mass splitting between the the top and bottom quarks in a technicolor scenario. The model proposed here contains a left-right electroweak gauge group. An extended technicolor group and mirror fermions are introduced. The top-bottom quark mass splitting turns out to be intimately connected to the breaking of the left-right gauge symmetry. Weak isospin violation occurs within the experimental limits.

Neutrino masses through a type II seesaw mechanism at TeV scale

In this work we show that we can generate neutrino masses through the type II seesaw mechanism working at TeV scale in the context of a 331 model. (C) 2001 Published by Elsevier B.V. B.V.

Instant preheating mechanism and ultrahigh energy cosmic rays

Top-down models assume that the still unexplained ultrahigh energy cosmic rays (UHECR's) are the decay products of superheavy particles. Such particles may have been produced by one of the post-inflationary reheating mechanisms and may account for a fraction of the cold dark matter. In this paper, we assess the phenomenological applicability of the simplest instant preheating framework not to describe a reheating process, but as a mechanism to generate relic supermassive particles as possible sources of UHECR's. We use cosmic ray flux and cold dark matter observational data to constrain the parameters of the model.

On the HBO mechanism of X-ray neutron star

We ascribe the 15-60 Hz Quasi Periodic Oscillation (QPO) to the periastron precession frequency of the orbiting accreted matter at the boundary of magnetosphere-disk of Xray neutron star (NS). Considering the relativistic motion mechanism for the kHz QPO, that the radii of the inner disk and magnetosphere-disk of NS are correlated with each other by a factor is assumed. The obtained conclusions include: all QPO frequencies increase with increasing the accretion rate. The theoretical relations between 15-60 Hz QPO (HBO) frequency and the twin kHz QPOs are similar to the measured empirical formula. Further, the better fitted NS mass by the proposed model is about 1.9 solar masses for the detected LMXBs.

Neutrino masses through the seesaw mechanism in 3-3-1 models

Some years ago it was shown by Ma that in the context of the electroweak standard model there are, at the tree level, only three ways to generate small neutrino masses by the seesaw mechanism via one effective dimension-five operator. Here we extend this approach to 3-3-1 chiral models showing that in this case there are several dimension-five operators and we also consider their tree level realization.