Modelos de cura: aplicação ao cancro da mama feminino

A Análise de Sobrevivência tem como objetivo o estudo do tempo desde um instante inicial bem definido até ao acontecimento de determinado evento. Por exemplo, poderá ser o tempo de vida de um indivíduo desde o momento em que lhe é diagnosticada uma doença até a sua morte ou cura. Com a evolução da medicina, começou a se verificar a existência de indivíduos para os quais nunca se observava o acontecimento de interesse e designaram-se esses indivíduos por curados, imunes, ou não suscetíveis. Assim, da Análise de Sobrevivência clássica surgem os modelos de cura. Neste trabalho, aplicaram-se estes conceitos a uma base de dados referentes a 833 mulheres diagnosticadas com cancro da mama, entre 1998 e 2005. Verificou-se a existência de um risco de morte maior em mulheres na faixa etária dos 50 a 59 anos. Comprovou-se que o estadiamento tem um papel preponderante em relação ao prognóstico, sendo que, quanto mais avançado o estadio pior o prognóstico. Dos tratamentos a que os doentes foram submetidos, a realização de cirurgia é indicativa de um melhor prognóstico, assim como a realização de hormonoterapia e de radioterapia. No entanto, este último tratamento não se revelou estatisticamente significativo para o modelo de regressão de Cox. A realização de quimioterapia apenas reflete um melhor prognóstico nos primeiros dois anos, o que já não acontece a partir dai. Esta caraterística inesperada ficou-se a dever à esperança de vida que o tratamento oferece aos doentes no estadio IV e da associação entre a existência de gânglios metastizados e o agravamento do prognóstico, no caso do estadio II. O modelo de cura foi aplicado apenas ao grupo de mulheres no estadio IV, pois só neste caso se admitiu que o tempo de follow-up era suficiente, obtendo-se uma taxa de cura de 7;4%.

Indução de morte celular em células de adenocarcinoma cervical humano (HeLa) pela lectina da esponja Cinachyrella apion (CaL)

Cancer is a term used to represent a set of more than 100 diseases, including malignant tumors from different locations. The malignancies are the second leading cause of death in the population, representing approximately 17% of deaths of known cause. Strategies that induce differentiation have had limited success in the treatment of established cancers. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated due to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death via apoptosis in tumor cells. The antiproliferative activity of CaL was tested against cell lines, with the highest inhibition of tumor growth for HeLa, reducing cell growth at a dose dependent manner, with a concentration of 10 μg/mL. The hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. The results showed the lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase, with accumulation of cells of approximately 57% in this phase, and acting as both dependent and/or independent of caspases pathway. These results suggest that CaL has the potential to be used as drug treatment against cancer.

Simulador radiográfico antropomórfico de mama humana

Mammography is a diagnostic imaging method in which interpretation depends on knowledge of radiological aspects as well as the clinical exam and pathophysiology of breast diseases. In this work a mammography phantom was developed to be used for training in the operation of mammographic x-ray equipment, image quality evaluation, self-examination and clinical examination of palpation. Polyurethane was used for the production of the phantoms for its physical and chemical properties and because it is one of the components normally used in prostheses. According to the range of flexibility of the polyurethane, it was possible to simulate breasts with higher or lower amount of adipose tissue. Pathologies such as areolar necrosis and tissue rejection due to surgery reconstruction after partial mastectomy were also simulated. Calcifications and nodules were simulated using the following materials: polyethylene, poly (methyl methacrylate), polyamide, polyurethane and poly (dimethyl silicone). Among these, polyethylene was able to simulate characteristics of calcification as well as breast nodules. The results from mammographic techniques used in this paper for the evaluation of the phantoms are in agreement with data found in the literature. The image analyses of four phantoms indicated significant similarities with the human skin texture and the female breast parenchyma. It was possible to detect in the radiographic images produced regions of high and low radiographic optical density, which are characteristic of breasts with regions of different amount of adipose tissue. The stiffnesses of breast phantoms were adjusted according to the formulation of the polyurethane which enabled the production of phantoms with distinct radiographic features and texture similar to human female breast parenchyma. Clinical palpation exam of the phantoms developed in this work indicated characteristics similar to human breast in skin texture, areolar region and parenchyma

Biodistribuição de samário -153- EDTMP em ratos TRATADOS com docetaxel

A dor óssea decorrente das metástases é um sintoma comum nos tumores avançados de mama e próstata. Nenhuma opção terapêutica isolada é completamente eficaz, e uma série de modalidades costuma ser empregada, entre eles a terapia com radiofármacos, como o samário153-etilenodiaminatetrametileno fosfonato (EDTMP-153Sm). O docetaxel, um taxano com ação sobre tumores avançados de mama e próstata, tem-se apresentado como uma nova opção de tratamento quimioterápico. Muitos pacientes fazem uso simultâneo de EDTMP-153Sm e docetaxel. Este estudo procurou avaliar a influência do docetaxel na biodisponibilidade de EDTMP-153Sm em ratos Wistar, aleatoriamente alocados em 2 grupos de 6 animais cada. O grupo DS (docetaxel/samário) recebeu docetaxel (15 mg/kg) intraperitoneal em dois ciclos com 11 dias de intervalo. Os ratos do grupo S (samário/controle) não foram tratados com docetaxel. Nove dias após a quimioterapia, todos os animais receberam 0.1ml de EDTMP-153Sm via plexo orbital (25μCi). Após 2 horas, os animais foram mortos, e realizaram-se análises de amostras de cérebro, tireóide, pulmão, coração, estômago, cólon, fígado, rim e fêmures. O percentual de radioatividade por grama (%ATI/g) de tecido de cada biópsia foi determinado em contador gama automático (Wizard-1470, Perkin-Elmer, Finland). No 9º dia após 2º ciclo de docetaxel, os ratos tiveram perda de peso significante, passando de 353.66± 22.8g (controle/pré-tratamento) para 314,50±22,09g (p<0.5). Os %ATI/g nos órgãos dos ratos tratados com EDTMP- 153Sm e docetaxel tiveram redução significante nos fêmures direito e esquerdo, rim, fígado e pulmão, quando comparados aos animais não tratados com docetaxel. Em conclusão, a combinação de docetaxel com EDTMP-153Sm foi associada à menor concentração do radiofármaco em órgãos alvo. Futuras investigações sobre o impacto do docetaxel na biodisponibilidade do EDTMP- 153Sm poderão complementar estes achados. Deve-se ressaltar o caráter multidisciplinar deste estudo, que contou com a participação ativa e com a troca constante de conhecimentos entre profissionais das áreas de Medicina Nuclear, Cirurgia, Oncologia, Biologia e Estatística

Abordagem inicial de lesões mamárias por biopsia helicóide: estudo experimental

OBJECTIVE: Evaluating the kit-Bh performance in carrying out of breast biopsies. METHODS: They were randomly selected a sample of 30 patients with breast cancer undergoing mastectomy, based on the results of a pilot study from February 2008 to April 2010. They were excluded women with had not palpable, stone-hard consistency tumors, previous surgical manipulation or that contains liquid. Using the helicoid biopsy Kit (kit Bh) and an equipment Core biopsy with cannula and needle and 14 gauge respectively, it was collected a fragment of sound equipment in the area and in tumors in each specimen, totaling 120 fragments for histological study. For data analysis, it was defined a 95% confidence level and used the SPSS-13 version, the Kappa index and the parametric Student t test. RESULTS: Mean age of patients was 51.6 years (± 11.1 years). The infiltrating ductal carcinoma showed a higher incidence, 26 cases (86.7%). The Core biopsy had a sensitivity of 93.3%, specificity of 100% and accuracy 96.7%, while the helicoid biopsy had a sensitivity of 96.7%, specificity of 100% and accuracy 98.3%. By comparing the histology of tumors and the fragments of biopsies, there was high degree of agreement in diagnoses (kappa of 0.93 with p <0.05) CONCLUSION: Both devices provided the histological diagnosis of lesions with high accuracy. Results of this study showed that the helicoid biopsy is a reliable alternative in 22 the preoperative diagnosis of breast lesions. Further studies in vivo better will define the role of Kit Bh in the diagnosis of these lesions

Avaliação dos efeitos da atividade física na prevenção do estresse oxidativo e do câncer de mama

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Análise de depoimentos de mulheres mastectomizadas sobre o câncer de mama

The breast cancer is the most incident neoplasia in Brazilian women, configure as important cause of female death in Brazil. Its magnitude is go to be consider as a disease that go out the biological and numerical, extending of the subjective dimension and interrelationships of society and socials experiences, to into in knowledge and practices. Linking of the growing older process, the breast cancer extend as social, economical and cultural dimensions, madding in plot of socials relationships, through that acquire mean. In the context of the high expectative of live and the high number of older age persons, consider that that the high number of years had lived correspond the exposition of this individuals to corporate of the ambientals aggressions and own processes of human constitution of natural wear, like as chronicle-degenerate disease, the example is cancer. In the perspective, we collected narratives of mastectomies women with 60 age or so, about the breast cancer, the body and the growing older process, had has as objectives to reflexes about relatives questions, the comprehension about cancer, like experience, had lived and mean in the context of action and interaction of mastectomies older women, and to comprehend like women interrelationships and respond the changes that grow up of the disease and the growing older process in the everyday of their lives. Through of the narratives. We know that the disease is an initial information that take a form through successives approximation between women with their reality, since the family from hospital institution. The breast cancer to be continue a disease who cause a lot of apprehension and fear, getting and changing the ill s live as marked form. Have dad the body and the growing older process genteels and redimensions by disease, women need to define news and multiples functions due to the contingency that the disease impose