Análise do potencial mutagênico dos esteroides anabólicos androgênicos (EAA) e da l-carnitina mediante o teste do micronúcleo em eritrócitos policromáticos

INTRODUCTION: Anabolic androgenic steroids (AAS) are frequently used by people whose aim to increase muscle mass to obtain a better performance in sports or improve physical appearance. AAS are synthetic derivatives of testosterone, able to promote muscle fibers hypertrophy, increasing intracellular protein synthesis. L-carnitine is a food supplement used to increase energetic production by means of fat acids oxidation. Although there are several works about physiological properties of these drugs, there are few studies about their mutagenic potential. OBJECTIVES: This work evaluated the clastogenicity and genotoxicity of nandrolone decanoate, testosterone decanoate and L-carnitine, in different treatments through the micronucleus test in polychromatic erythrocytes of Wistar rats. METHODS: The animals were submitted to different concentrations and associations of AAS. The positive control received cyclophosphamide 50 mg/kg by intraperitoneal injection and negative control, one ml of saline solution by gavage. The rats were sacrificed after 36 hours of latest application, having the femurs removed and the bone marrow extracted. Material was homogenized and centrifuged. Button cell was pipetted and transferred to slides, which were stained by Giemsa. 1,000 polychromatic erythrocytes were counted per animal, noting the frequency of micronuclei. RESULTS: The Kruskal-Wallis test was performed, with a significance level of 5%, which demonstrated that nandrolone decanoate - three doses of 0,2 mg/kg and 0,6 mg/kg, eight doses of 7,5 mg/kg, L-carnitine - seven doses of 0,4 ml/250 g and 1,5 ml/250 g, testosterone decanoate - 28 doses of 0,075 mg/kg, nandrolone decanoate - eight doses of 7,5 mg/kg associated to L-carnitine and 1 mL and nandrolone decanoate - eight doses of 7,5 mg/kg associated to testosterone decanoate - eight doses of 7,5 mg/kg, showed mutagenic potential. CONCLUSION: The treatments proved to be clastogenic, not being indicated like ergogenic aid.

Manejo do pasto de capim-marandu e suplementação com diferentes fontes de energia na recria de tourinhos Nelore

Pós-graduação em Zootecnia - FCAV

Desenvolvimento de metodologia analítica ambientalmente mais amigável para a determinação de ureía em atrizes diversas

Pós-graduação em Química - IQ

A diet enriched in linoleic acid compromises the cryotolerance of embryos from superovulated beef heifers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Supplementation based on protein or energy ingredients to beef cattle consuming low-quality cool-season forages: I. Forage disappearance parameters in rumen-fistulated steers and physiological responses in pregnant heifers

Two experiments evaluated the influence of supplement composition on ruminal forage disappearance, performance, and physiological responses of Angus x Hereford cattle consuming a low-quality cool-season forage (8.7% CP and 57% TDN). In Exp. 1, 6 rumen-fistulated steers housed in individual pens were assigned to an incomplete 3 x 2 Latin square design containing 2 periods of 11 d each and the following treatments: 1) supplementation with soybean meal (PROT), 2) supplementation with a mixture of cracked corn, soybean meal, and urea (68:22:10 ratio, DM basis; ENER), or 3) no supplementation (CON). Steers were offered meadow foxtail (Alopecurus pratensis L.) hay for ad libitum consumption. Treatments were provided daily at 0.50 and 0.54% of shrunk BW/steer for PROT and ENER, respectively, to ensure that PROT and ENER intakes were isocaloric and isonitrogenous. No treatment effects were detected on rumen disappearance parameters of forage DM (P >= 0.33) and NDF (P >= 0.66). In Exp. 2, 35 pregnant heifers were ranked by initial BW on d -7 of the study, allocated into 12 feedlot pens (4 pens/treatment), and assigned to the same treatments and forage intake regimen as in Exp. 1 for 19 d. Treatments were fed once daily at 1.77 and 1.92 kg of DM/heifer for PROT and ENER, respectively, to achieve the same treatment intake as percent of initial BW used in Exp. 1 (0.50 and 0.54% for PROT and ENER, respectively). No treatment effects (P = 0.17) were detected on forage DMI. Total DMI was greater (P < 0.01) for PROT and ENER compared with CON and similar between PROT and ENER (P = 0.36). Accordingly, ADG was greater (P = 0.01) for PROT compared with CON, tended to be greater for ENER compared with CON (P = 0.08), and was similar between ENER and PROT (P = 0.28). Heifers receiving PROT and ENER had greater mean concentrations of plasma glucose (P = 0.03), insulin (P <= 0.09), IGF-I (P <= 0.04), and progesterone (P = 0.01) compared to CON, whereas ENER and PROT had similar concentrations of these variables (P >= 0.15). A treatment x hour interaction was detected (P < 0.01) for plasma urea N (PUN), given that PUN concentrations increased after supplementation for ENER and PROT (time effect, P < 0.01) but did not change for CON (time effect, P = 0.62). In conclusion, beef cattle consuming low-quality cool-season forages had similar ruminal forage disappearance and intake, performance, and physiological status if offered supplements based on soybean meal or corn at 0.5% of BW.

Performance and carcass quality of feedlot- or pasture-finished Nellore heifers according to feeding managements in the postweaning phase

The objective of this study was to evaluate the postweaning history of heifers kept on marandu grass pastures with three canopy heights, in a continuous-grazing system, during the rainy period, on feedlot-or pasture finishing. The effects of three canopy heights (15, 25 and 35 cm) associated with two supplements (mineral salt and protein-energy supplement) and two finishing systems in the dry period (feedlot and open pasture) were studied in the postweaning period during the rainy season. The adopted design was completely randomized, with seven replications (animals) in a 2 x 2 factorial arrangement. The animals which received protein-energy supplement reached the finishing period with greater body weight in relation to those fed mineral salt. In both feedlot and pasture finishing systems, compensatory gain effect was observed in the animals that remained on the low pastures during postweaning compared with the high pastures. This compensatory gain was not verified in the animals that received protein-energy supplement in relation to the mineral salt, and thus the animals were slaughtered beforehand. The heifers on pastures with 25 or 35 cm in height were slaughtered in the same period, and those kept on the low pastures were slaughtered afterwards. The studied factors in the postweaning phase did not affect the carcass characteristics. Animals finished in feedlot slaughtered with the same body weight as those finished on pasture show greater carcass yield, subcutaneous fat deposition and renal pelvic and inguinal fat and greater losses with trimmings for cleaning the main hindquarter meat cuts; however, they provide cuts with the same weight but greater fat cover.

Comparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência do estado nutricional e da composição corporal na evolução clínica dos pacientes com doença inflamatória intestinal

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Biodisponibilidade de flavanonas e atividade antioxidante do suco de laranja fresco versus suco de laranja pasteurizado em humanos saudáveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Performance of steers supplemented with mineral mixtures in the State of Mato Grosso

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Consumo alimentar residual e produção de metano entérico de bovinos em confinamento e pastagem

Pós-graduação em Zootecnia - FCAV

Corrective effects of acerola (Malpighia emarginata DC.) juice intake on biochemical and genotoxical parameters in mice fed on a high-fat diet

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Vacuum polarization by a magnetic flux in a cosmic string background.

In this paper we analyse the vacuum polarization effects due to a magnetic flux on massless fermionic fields in a cosmic string background. Three distinct configurations of magnetic fields are considered. In all of them the magnetic fluxes are confined in a long cylindrical tube of finite radius.

Preposições em textos do gênero notícia na imprensa paulista: norma e uso no ínicio do século XX

This paper proposes a reflection on processes of variation and change that occurred with the portuguese prepositions a, ate, em and para, taken as a parameter for answers in a broader context, the confrontation between the norm and use and between standard and linguistic variation. We conducted the study based on a corpus composed of all occurrences of the prepositions in question removed news of the newspaper O Combate and O Estado de Sao Paulo. Whereas the main objective of the study is to present and describe the use of these particles in newspaper articles of the Imprensa Paulista, data were collected and quantified by using the statistical package GOLDVARB, and interpretation of results relied (i) assumptions regarding the process variation in the use of prepositions and prepositions replace weak, as the preposition to, for strong prepositions (para, em, até), (ii) in comparison with the grammatical rule in effect at the time, (iii) and in the search for the existence of some historical factor that justifies the selection of a preposition rather than another. Among the hypotheses, we investigate the relationship between these prepositions and whose kind of preachers they introduce supplements: the direction of motion (abstract or concrete) or transfer (material and verbal / perceptual). This theoretical perspective was the Labovian sociolinguistics (Labov, 1972, 1994), which defines the linguistic variable as a representation of two or more different ways to convey a certain information content, being necessary to define it the following criteria set exact number of variants to establish all the multiplicity of contexts in which it appears; develop a quantitative index for measuring the values of variables

Entre o acadêmico e o jornalístico: análise da coluna de crítica literária Prosa de Sábado, do suplemento Sabático

The literary criticism was developed in Brazil in the period that comprehends the end of the 19th century and the beginning of the 20th century. During that time, the mean of dissemination of the criticism were the footnotes of the newspapers, places in which literature was discussed and the critics expressed their personal impressions about literary works with the objective of orientating the good taste of the readers. With the progressive evolution of journalism and of the newspaper's audience, the criticism columns became incompatible with the informative part of the periodicals, being transferred to the literary supplements, during a transition phase, until it was concentrated in the universitary field. This change in the paradigms of the production of criticism also caused a change in the profile of the literary critics and in the criteria to do the criticism. Before, if the critics were professionals that only needed to have enough erudition to judge the literary works, they turn into literature experts and need to use scientific methodologies in the literary analysis, while the cultural journalist begins to look at the events and literary works as products which belong to a market that needs to be reported. This research proposes studying and comparing the critics published in the column Prosa de Sábado, of the literary supplement Sabático, produced by Silviano Santiago, critic of an academic origin, and Sérgio Augusto, journalist critic, with the objective of identifying similarities and contrasts between them, and analyse the relation of this critics with their distinct fields of literary legitimation, as well as reflecting about the presence of the literary criticism and of the critics in the current press