959 resultados para Pigments. Polymerization Complexes method. Fractional factorial design


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This work discusses the design of flexible pavements for airports, with the aid techniques and tools necessary for the calculation and design critical aircraft as the Boeing 777-300ER. Also discussed the layers that make up the pavement, the materials used and the method used for design pavement (FAA method)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Agricultura) - FCA

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Pós-graduação em Agronomia (Agricultura) - FCA

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Pós-graduação em Zootecnia - FCAV

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Pós-graduação em Agronomia (Produção Vegetal) - FCAV

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Pós-graduação em Zootecnia - FCAV

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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Pós-graduação em Agronomia (Produção Vegetal) - FCAV

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Objective To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension. Results A multilevel factorial design was used to quantify effects of temperature (33–37 C), fresh medium addition after the viral adsorption step (100–200 % with respect to the initial cell suspension volume before infection) and harvest time (8–40 h) on RVGP production. Experimental runs were performed in 24-well cell culture plates at a multiplicity of infection (MOI) of 16. An additional experiment in spinner-flask was performed at MOI of 9, using the optimal conditions determined in cell culture plates. Values for temperature, fresh medium addition and harvest time of 33 C, 100 % and 16 h, respectively, ensured the optimal RVGP production in culture plates. The volumetric yield (239 ng ml-1 ) in these conditions was higher than that reported previously for adherent cell culture. In spinner-flasks, the volumetric yield was improved (559 ng ml-1 ). Conclusion These results establish the basis for designing bioprocess to produce RVGP.

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This work aimed to compare the predictive capacity of empirical models, based on the uniform design utilization combined to artificial neural networks with respect to classical factorial designs in bioprocess, using as example the rabies virus replication in BHK-21 cells. The viral infection process parameters under study were temperature (34°C, 37°C), multiplicity of infection (0.04, 0.07, 0.1), times of infection, and harvest (24, 48, 72 hours) and the monitored output parameter was viral production. A multilevel factorial experimental design was performed for the study of this system. Fractions of this experimental approach (18, 24, 30, 36 and 42 runs), defined according uniform designs, were used as alternative for modelling through artificial neural network and thereafter an output variable optimization was carried out by means of genetic algorithm methodology. Model prediction capacities for all uniform design approaches under study were better than that found for classical factorial design approach. It was demonstrated that uniform design in combination with artificial neural network could be an efficient experimental approach for modelling complex bioprocess like viral production. For the present study case, 67% of experimental resources were saved when compared to a classical factorial design approach. In the near future, this strategy could replace the established factorial designs used in the bioprocess development activities performed within biopharmaceutical organizations because of the improvements gained in the economics of experimentation that do not sacrifice the quality of decisions.

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With the increase in world population and scarcity of natural resources, efficient use of fertilizers becomes necessary for intensive agriculture. The experiment was conducted in a greenhouse at the Department of Agricultural Engineering, UNESP in Botucatu-SP. The treatments were derived from the combination of the soil salinity (E.C: 1.0, 3.0, 6.0, 9.0 and 12.0 dS m-1), Fertigation management (M1 =traditional and M2 = with control of the ionic concentration of the soil solution) and beet cultivars (C1= Early Wonder and C2 = Itapuã) in a 5x2x2 factorial design with four replications in a randomized block design. Throughout the cultivation, the following variables were evaluated: height, stem diameter, length and diameter of plant roots. The height of the plant presented differently according to the Fertigation management and sensitive to levels of electrical conductivity in the soil. The diameter of the roots showed reductions of 3.55 and 2.48 mm for C1 and C2, respectively, every unit increase in electrical conductivity (EC) to M1. Based on the functional relationship of the best adjustment between the diameter of the roots and electrical conductivity in M2 gave an estimated maximum diameter of 90.78 mm to 94.67 mm for C1 and C2.

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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

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Inorganic phosphorus (Pi) usually controls the P availability in tropical soils, but the contribution of organic P (Po) should not be neglected, mainly in systems with low P input or management systems that promote organic matter accumulation. The aims of this study were to evaluate the changes in the Po fractions over time in soil fertilized and not fertilized with cattle manure and to correlate Po forms with available P extracted by anion exchange resin. The experiment was carried out under field conditions, in a sandy-clay loam Haplustox. The experimental design was a 2 x 9 randomized complete block factorial design, in which the first factor was manure application (20 t ha(-1)) or absence, and the second the soil sampling times (3, 7, 14, 21, 28, 49, 70, 91, and 112 days) after manure incorporation. Labile, moderately labile and non-labile Po fractions were determined in the soil material of each sampling. Manure fertilization increased the Po levels in the moderately labile and non-labile fractions and the total organic P, but did not affect the Po fraction proportions in relation to total organic P. On average, 5.1 % of total Po was in the labile, 44.4 % in the moderately labile and 50.5 % in the non-labile fractions. Available P (resin P) was more affected by the manure soluble Pi rather than by the labile Po forms. The labile and non-labile Po fractions varied randomly with no defined trend in relation to the samplings; for this reason, the data did not fit any mathematical model.