Luciferase de Macrolampis SP como gene repórter dual em biosensores simultâneos de expressão gênica e variações de intracelulares de ph, concentrações de fosfato e cátions divalentes de metais pesados

Trata-se a presente invenção de um produto da área de biotecnologia constituído de Luciferase de Microlampis SP recombinante apresenta espectro de bioluminescência bimodal e sensibilidade elevada a mudanças de pH- e temperatura, como gene repórter dual que codifica uma proteína bioluminosa para uso em biosensores simultâneos de expressão gênica e variações de intracelulares de PH, com concentrações de Fosfato e Cátions divalentes de metais pesados, e presença de ATP e como biosensor intracelular de mudanças de pH e outras condições homeostáticas.

Desenvolvimento de um eletrodo modificado com monocamadas auto-organizadas e sua utilização como biossesor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Síntese, caracterização e estudo da auto-associação em solução aquosa de derivados anfifílicos zwiteriônicos de quitosana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação espaço-temporal da disponibilidade hídrica subterrânea do município de São José do Rio Preto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparing label free electrochemical impedimetric and capacitive biosensing architectures

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Design, synthesis and characterization of a hexapeptide bio-inspired by acetylcholinesterase and its interaction with pesticide dichlorvos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stimuli-Responsive Biointerface Based on Polymer Brushes for Glucose Detection

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Electrochemical investigations on the capacity of flavonoids to protect DNA against damage caused by textile disperse dyes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Glutathione-s-transferase modified electrodes for detecting anticancer drugs

With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for measuring anticancer drugs, but currently few biosensors can achieve this goal. In particular, it is challenging to have a general method to monitor various types of anticancer drugs with different structures. In this work, a biosensor was developed to detect anticancer drugs by modifying carbon paste electrodes with glutathione-s-transferase (GST) enzymes. GST is widely studied in the metabolism of xenobiotics and is a major contributing factor in resistance to anticancer drugs. The measurement of anticancer drugs is based on competition between 1-chloro-2,4-dinitrobenzene (CDNB) and the drugs for the GST enzyme in the electrochemical potential at 0.1 V vs. Ag/AgCl by square wave voltammetry (SWV) or using a colorimetric method. The sensor shows a detection limit of 8.8 mu M cisplatin and exhibits relatively long life time in daily measurements. (C) 2014 Elsevier B.V. All rights reserved.

ANTIDIARRHEAL MECHANISM AND IONIC PROFILE OF CARPOLOBIA LUTEA ETHANOLIC STEM-BARK EXTRACT IN RATS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de biossensor eletroquímico para diagnóstico de dengue

Pós-graduação em Biotecnologia - IQ

Diagnostic tests for hepatitis C: Recent trends in electrochemical immunosensor and genosensor analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Geofísica aplicada no diagnóstico preliminar do antigo lixão do município de Rio Claro - SP

The issue of solid waste disposal and urban growth are conflictive, since the areas where old uncontrolled refuse disposal were away from the city today are incorporated to the urban area population. Thus, now population is closer to the degradation of contaminants coming from waste disposal, and, in some cases, these areas become housing subdivisions. This work aims to support the diagnosis of a former landfill in the municipality of Rio Claro (SP), using geophysical methods through resistivity anomalies. In order to develop the diagnostic, it was utilized water and soil analysis of a preliminary report and techniques of vertical electrical sounding and electrical profiling. The areas influenced by the garbage presented less resistivity than the one of the natural subsoil. Through the interpretation of results, the local potentiometric map was elaborated, as well as a map of calculated resistivity. The interpretation of these products indicated a disagreement between the groundwater flow and the current topography, leading to an identification of a preferential direction of the contamination plume in the NE-SW. These observations prove the applicability of geoelectrical methods in areas of former waste disposal with little information.

Estudo das propriedades estímulo responsivas dos polímeros escovas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)