Expression Studies of the Zeaxanthin Epoxidase Gene in Nicotiana plumbaginifolia1

Abscisic acid (ABA) is a plant hormone involved in the control of a wide range of physiological processes, including adaptation to environmental stress and seed development. In higher plants ABA is a breakdown product of xanthophyll carotenoids (C40) via the C15 intermediate xanthoxin. The ABA2 gene of Nicotiana plumbaginifolia encodes zeaxanthin epoxidase, which catalyzes the conversion of zeaxanthin to violaxanthin. In this study we analyzed steady-state levels of ABA2 mRNA in N. plumbaginifolia. The ABA2 mRNA accumulated in all plant organs, but transcript levels were found to be higher in aerial parts (stems and leaves) than in roots and seeds. In leaves ABA2 mRNA accumulation displayed a day/night cycle; however, the ABA2 protein level remained constant. In roots no diurnal fluctuation in mRNA levels was observed. In seeds the ABA2 mRNA level peaked around the middle of development, when ABA content has been shown to increase in many species. In conditions of drought stress, ABA levels increased in both leaves and roots. A concomitant accumulation of ABA2 mRNA was observed in roots but not in leaves. These results are discussed in relation to the role of zeaxanthin epoxidase both in the xanthophyll cycle and in the synthesis of ABA precursors.

Forest losses predict bird extinctions in eastern North America.

Claims that there will be a massive loss of species as tropical forests are cleared are based on the relationship between habitat area and the number of species. Few studies calibrate extinction with habitat reduction. Critics raise doubts about this calibration, noting that there has been extensive clearing of the eastern North American forest, yet only 4 of its approximately 200 bird species have gone extinct. We analyze the distribution of bird species and the timing and extent of forest loss. The forest losses were not concurrent across the region. Based on the maximum extent of forest losses, our calculations predict fewer extinctions than the number observed. At most, there are 28 species of birds restricted to the region. Only these species would be at risk even if all the forests were cleared. Far from providing comfort to those who argue that the current rapid rate of tropical deforestation might cause fewer extinctions than often claimed, our results suggest that the losses may be worse. In contrast to eastern North America, small regions of tropical forest often hold hundreds of endemic bird species.

Census of breeding birds and population trends of the Dupont’s lark Chersophilus duponti in eastern Spain

We present the results of a breeding census of Dupont’s Lark carried out in the Autonomous Community of Valencia in 2011 using the mapping method and an analysis of the recent trend for this population. The population was estimated at 44-47 males, located in five habitat patches of the Rincón de Ademuz, in zones where the species had been detected previously. Density in these patches fits within values expected according to the relation between patch size and density found in the populations of the nearby provinces. Comparison to previous studies reveals that population of Valencia has suffered an annual decline rate of around 10% on average, thus the implementation of a conservation plan is required to ensure the survival of the species in the study area.

Paleogene evolution of the External Betic Zone and geodynamic implications

Interdisciplinary studies combining field data (geological and tectonic mapping, lithostratigraphic reconstructions, lithofacies characterization, correlations and sampling) and laboratory analyses (biostratigraphy, chronostratigraphy, clay mineralogy and sandstone petrography) of eight Senonian-Paleogene successions from the Sierra de La Pila and Sierra de El Carche areas (Murcia province, SE Spain) belonging to the External Betic Zone are presented. Field evidence of tectonic activity (slumps, olistostromes, syn-genetic folds, lateral variability, changes in thicknesses, para- and unconformity boundaries, stratigraphic gaps, shallowing upward trends to emersion, etc.) was found in several Paleogene intervals. The results enable a better reconstruction of the stratigraphic architecture and chronostratigraphy of the Paleogene record, highlighting in particular: facies evolution, discontinuities, depositional sequences (Middle-Upper Maastrichtian, Upper Paleocene-Middle Eocene, Oligocene-Lower Aquitanian), environmental evolution (homogeneous conditions during the Late Cretaceous and successive realm diversification from platform to slope to basin) and correlations, along the Prebetic to Subbetic transition, which is a key sector to understand the northeastward variations of the South Iberian margin. A conclusive paleogeographic and geodynamic evolutionary model for the study area is proposed, hypothesizing that Paleogene compressive tectonics affected the eastern External Betic Zone. In addition, correlations with successions from the western External Betic Zone evidenced asynchronous deformation from east to west along the internalmost External Betic Zone. Moreover, a comparison with the external Tunisian Tell enables the recognition of similar sedimentarytectonic events, imposing new constraints in the Paleogene geodynamic reconstruction throughout the western Tethys.

Amphibian Hotspots and Conservation Priorities in Eastern Cuba Identified by Species Distribution Modeling

The high rate of amphibian endemism and the severe habitat modification in the Caribbean islands make them an ideal place to test if the current protected areas network might protect this group. In this study, we model distribution and map species richness of the 40 amphibian species from eastern Cuba with the objectives of identify hotspots, detect gaps in species representation in protected areas, and select additional areas to fill these gaps. We used two modeling methods, Maxent and Habitat Suitability Models, to reach a consensus distribution map for each species, then calculate species richness by combining specific models and finally performed gap analyses for species and hotspots. Our results showed that the models were robust enough to predict species distributions and that most of the amphibian hotspots were represented in reserves, but 50 percent of the species were incompletely covered and Eleutherodactylus rivularis was totally uncovered by the protected areas. We identified 1441 additional km2 (9.9% of the study area) that could be added to the current protected areas, allowing the representation of every species and all hotspots. Our results are relevant for the conservation planning in other Caribbean islands, since studies like this could contribute to fill the gaps in the existing protected areas and to design a future network. Both cases would benefit from modeling amphibian species distribution using available data, even if they are incomplete, rather than relying only in the protection of known or suspected hotspots.

Ancient seals of the Near East / by Richard A. Martin, Curator of Near Eastern Archaeology.

no.34(1940)

Turkey, Middle East, and the South Caucasus, 1771 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Turcia Asiatica : exhibens Natoliam modernam, in suos beglirbegatus divisam, itemque reliquos beglirbegatus, sive gubernationes et Prfecturas generales, in regionibus Georgiæ, Armeniæ, Mespotamiæ, Syriæ et Arabiæ sitos, una cum Mari Nigro, Mari Azowiensi, Mari di Marmora, et Mari Ægeo, atque adiacentibus Insulis : C. P. S. C. M. = Carte de la Turquie asiatique contenant la Natolie moderne divisée en les beglerbeys &c., ex novissimis subsidiis ac relationibus ad normam legitimae proiectionis in usum belli praesentis delineata impensis homannianorum heredum. It was published by Homannianorum Heredum in 1771. Scale [ca. 1:4,800,000]. Covers Turkey and portions of the Middle East and the South Caucasus. Map in Latin with title in French. The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial and provincial boundaries, shoreline features, and more. Relief shown pictorially. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection as part of the Open Collections Program at Harvard University project: Islamic Heritage Project. Maps selected for the project represent a range of regions, originators, ground condition dates, scales, and purposes. The Islamic Heritage Project consists of over 100,000 digitized pages from Harvard's collections of Islamic manuscripts and published materials. Supported by Prince Alwaleed Bin Talal and developed in association with the Prince Alwaleed Bin Talal Islamic Studies Program at Harvard University.

Middle East, 1853 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Syria, by W. Hughes. It was published by George Cox, Jan. 1st, 1853. Scale [ca. 1:2,200,000]. Covers a portion of the Middle East including all or portions of Syria, Lebanon, Israel, Gaza Strip, West Bank, Jordan, Turkey, Iraq, Saudi Arabia, and Egypt. The image inside the map neatline is georeferenced to the surface of the earth and fit to a modified 'Europe Lambert Conformal Conic' projection with a central meridian of 38 degrees East projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, and more. Relief shown by hachures. Includes note and inset: Continuation from the Dead Sea top Mount Sinai (Scale [ca. 1:2,200,000]). This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection as part of the Open Collections Program at Harvard University project: Islamic Heritage Project. Maps selected for the project represent a range of regions, originators, ground condition dates, scales, and purposes. The Islamic Heritage Project consists of over 100,000 digitized pages from Harvard's collections of Islamic manuscripts and published materials. Supported by Prince Alwaleed Bin Talal and developed in association with the Prince Alwaleed Bin Talal Islamic Studies Program at Harvard University.

Arabian Peninsula and Middle East, 1654 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carte des trois Arabies : tirée en partie de l'Arabe de Nubie, en partie de diuers autres autheurs, par le S. N. Sanson d'Abbeville Geographe ordinaire du Roy ; J. Somer sculp. It was published by Chez Pierre Mariette in 1654. Scale [ca. 1:7,500,000]. Map in French and Latin. Covers the Arabian Peninsula. The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, and more. Relief shown pictorially. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection as part of the Open Collections Program at Harvard University project: Islamic Heritage Project. Maps selected for the project represent a range of regions, originators, ground condition dates, scales, and purposes. The Islamic Heritage Project consists of over 100,000 digitized pages from Harvard's collections of Islamic manuscripts and published materials. Supported by Prince Alwaleed Bin Talal and developed in association with the Prince Alwaleed Bin Talal Islamic Studies Program at Harvard University.

Middle East, Tigris and Euphrates River Region, 1779 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: L'Euphrate et le Tigre, par le Sr. d'Anville ; Guill. De La Haye. It was published in 1779. Scale [ca. 1:2,400,000]. Cover the Euphrates and Tigris River region including portions of Iraq, Iran, Kuwait, Turkey, Syria, Jordan, and Lebanon. Map in French and Latin. The image inside the map neatline is georeferenced to the surface of the earth and fit to the a modified 'Europe Lambert Conformal Conic' projection with a central meridian of 44 degrees East projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, and more. Relief shown pictorially. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection as part of the Open Collections Program at Harvard University project: Islamic Heritage Project. Maps selected for the project represent a range of regions, originators, ground condition dates, scales, and purposes. The Islamic Heritage Project consists of over 100,000 digitized pages from Harvard's collections of Islamic manuscripts and published materials. Supported by Prince Alwaleed Bin Talal and developed in association with the Prince Alwaleed Bin Talal Islamic Studies Program at Harvard University.

Robotic Outcome Assessment in a Non-Human Primate Model of Middle Cerebral Artery Stroke

Stroke is a prevalent disorder with immense socioeconomic impact. A variety of chronic neurological deficits result from stroke. In particular, sensorimotor deficits are a significant barrier to achieving post-stroke independence. Unfortunately, the majority of pre-clinical studies that show improved outcomes in animal stroke models have failed in clinical trials. Pre-clinical studies using non-human primate (NHP) stroke models prior to initiating human trials are a potential step to improving translation from animal studies to clinical trials. Robotic assessment tools represent a quantitative, reliable, and reproducible means to assess reaching behaviour following stroke in both humans and NHPs. We investigated the use of robotic technology to assess sensorimotor impairments in NHPs following middle cerebral artery occlusion (MCAO). Two cynomolgus macaques underwent transient MCAO for 90 minutes. Approximately 1.5 years following the procedure these NHPs and two non-stroke control monkeys were trained in a reaching task with both arms in the KINARM exoskeleton. This robot permits elbow and shoulder movements in the horizontal plane. The task required NHPs to make reaching movements from a centrally positioned start target to 1 of 8 peripheral targets uniformly distributed around the first target. We analyzed four movement parameters: reaction time, movement time (MT), initial direction error (IDE), and number of speed maxima to characterize sensorimotor deficiencies. We hypothesized reduced performance in these attributes during a neurobehavioural task with the paretic limb of NHPs following MCAO compared to controls. Reaching movements in the non-affected limbs of control and experimental NHPs showed bell-shaped velocity profiles. In contrast, the reaching movements with the affected limbs were highly variable. We found distinctive patterns in MT, IDE, and number of speed peaks between control and experimental monkeys and between limbs of NHPs with MCAO. NHPs with MCAO demonstrated more speed peaks, longer MTs, and greater IDE in their paretic limb compared to controls. These initial results qualitatively match human stroke subjects’ performance, suggesting that robotic neurobehavioural assessment in NHPs with stroke is feasible and could have translational relevance in subsequent human studies. Further studies will be necessary to replicate and expand on these preliminary findings.

Phase progression throughout the therapeutic process : longitudinal case studies

Tese de doutoramento, Psicologia (Psicologia Clínica), Universidade de Lisboa, Faculdade de Psicologia, 2016

Inflation persistence in Central and Eastern European countries. Bruegel Working Paper 2013/04, 22 July 2013

This paper studies inflation persistence with time-varying coefficient autoregressions for twelve central European countries,in comparison with the United States and the euro area. Inflation persistence tends to be higher in times of high inflation. Since the oil price shocks, inflation persistence has declined both in the US and euro-area. In most central and eastern European countries, for which our study covers 1993-2012, inflation persistence has also declined, with the main exceptions of the Czech Republic, Slovakia and Slovenia, where persistence seems to be rather stable.

Geopolitical shifts in the Eastern Mediterranean. Security Policy Brief No. 43, February 2013

Three major geopolitical events are putting the stability of the Eastern Mediterranean at risk. Most of the region is in a deep monetary and economic crisis. The Arab Spring is causing turmoil in the Levant and the Maghreb. Gas and oil discoveries, if not well managed, could further destabilise the region. At the same time, Russia and Turkey are staging a comeback. In the face of these challenges, the EU approaches the Greek sovereign debt crisis nearly exclusively from a financial and economic viewpoint. This brief argues that the EU has to develop a comprehensive strategy for the region, complementing its existing multilateral regional framework with bilateral agreements in order to secure its interests in the Eastern Mediterranean.

The EuroNest Parliamentary Assembly: The European Parliament as a Socializer of its Counterparts in the EU’s Eastern Neighbourhood? EU Diplomacy Paper 05/2013

This paper examines the EU-Neighbourhood East Parliamentary Assembly (EuroNest PA), an inter-parliamentary forum consisting of representatives from the European Parliament (EP) and from all Eastern Partnership (EaP) countries except Belarus, aiming at political and economic association between the EU and the EaP. More specifically, it analyzes the extent to which the EP tries and manages to socialize the national parliaments of the EaP countries. After introducing the theoretical framework, the paper outlines the structure and working methods of the EuroNest PA, clarifies the absence of Belarus in the framework, and examines the results of the first three plenary sessions. The paper concludes that, although the establishment of the EuroNest PA as such provides a framework to advance the EP’s goals of transmitting its norms and values to the EU’s Eastern neighbouring countries, in practice socialization has taken place only to a limited extent thus far.