El Pisito 2011

Analyzing residential architecture in Spain today signifies tackling a complex, apparently fragmentary situation, one that makes comment or criticism quite difficult. In the last few years authority over housing and city planning has been transferred to the regions,1 and each has developed its own framework of laws, its own regulations and requirements, and has shaped urban and regional development on the basis of its own criteria. In parallel, schools of architecture have multiplied, reaching many of the zones previously regarded as ?peripheral.? It is easy to understand how the old administrative centrality has given way to a more pluralistic situation closer to the specific problems of each city and region. As a result cultural differences have been accentuated, even compromising the possibility of speaking of an ?architecture Spanish? or reducing it to a mere summation or mosaic of local circumstances. Part of the debate of recent years has been focused on underlining these differences. This essay takes the opposite approach. The premise from which it starts out is that analysis of the phenomenon of ?residential architecture in Spain? makes it possible to acknowledge the survival of a cultural unity that explains the present and the recent past and that does not conflict with the diversity of local experiences.

Il dibattimento: the debate: Teatro Romano Sagunto

Aportación al debate sobre el teatro romano de Sagunto

Cubierta para el parque arqueológico de El Molinete. Cartagena, Spain, 2008-2011

Cubierta para el parque arqueológico de El Molinete. Cartagena, Spain, 2008-2011

Expression of β-Amylase from Alfalfa Taproots1

Alfalfa (Medicago sativa L.) roots contain large quantities of β-amylase, but little is known about its role in vivo. We studied this by isolating a β-amylase cDNA and by examining signals that affect its expression. The β-amylase cDNA encoded a 55.95-kD polypeptide with a deduced amino acid sequence showing high similarity to other plant β-amylases. Starch concentrations, β-amylase activities, and β-amylase mRNA levels were measured in roots of alfalfa after defoliation, in suspension-cultured cells incubated in sucrose-rich or -deprived media, and in roots of cold-acclimated germ plasms. Starch levels, β-amylase activities, and β-amylase transcripts were reduced significantly in roots of defoliated plants and in sucrose-deprived cell cultures. β-Amylase transcript was high in roots of intact plants but could not be detected 2 to 8 d after defoliation. β-Amylase transcript levels increased in roots between September and October and then declined 10-fold in November and December after shoots were killed by frost. Alfalfa roots contain greater β-amylase transcript levels compared with roots of sweetclover (Melilotus officinalis L.), red clover (Trifolium pratense L.), and birdsfoot trefoil (Lotus corniculatus L.). Southern analysis indicated that β-amylase is present as a multigene family in alfalfa. Our results show no clear association between β-amylase activity or transcript abundance and starch hydrolysis in alfalfa roots. The great abundance of β-amylase and its unexpected patterns of gene expression and protein accumulation support our current belief that this protein serves a storage function in roots of this perennial species.