Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS

Listeria monocytogenes has previously been shown to adapt to a wide variety of environmental niches, principally those associated with low pH, and this compromises its control in food environments. An understanding of the mechanism(s) by which L. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to control the organism in foodstuffs. The present Study aimed to gain a further understanding of the physiological basis for the differential effects of one control strategy, namely the use of the lantibiotic nisin. Using propidium iodide (PI) to probe membrane integrity it was shown that L. monocytogenes Scott A was sensitive to nisin (8 ng mL(-1)) but this was growth phase dependent with stationary phase cells (OD600=1.2) being much more resistant than exponential phase cells (OD600=0.38). We demonstrate that, using a combination of techniques including fluorescence activated cell sorting (FACS), the membrane adaptations underpinning nisin resistance are triggered much earlier (OD600 < 0.5) than the onset of stationary phase. The significance of these findings in terms of mechanism and application are discussed. (c) 2005 Elsevier B.V.All rights reserved.

O DEBATE SOBRE A HISTÓRIA DAS ORIGENS DO TRABALHO BATISTA NO BRASIL Uma análise das relações e dos conflitos de gênero e poder na Convenção Batista Brasileira dos anos 1960-1980

O presente trabalho analisou as relações e os conflitos de gênero e poder observados durante o debate sobre as origens do trabalho batista no Brasil, debate esse entre o Pastor José dos Reis Pereira, líder oficial da Convenção Batista Brasileira durante os anos 1960-1980 e a pesquisadora batista Betty Antunes de Oliveira. A análise do conflito foi realizada principalmente com a mediação de gênero como instrumento hermenêutico, conforme os pressupostos de Joan Wallach Scott. Desse modo, a pesquisa teve como propósito principal, a partir da análise do debate, dar visibilidade ao conflito de gênero nos lugares de poder da Convenção Batista Brasileira dos anos 1960-1980, conflito dissimulado pelos discursos batistas sobre direitos de liberdade e igualdade sociais. Esta pesquisa trabalhou basicamente com as seguintes hipóteses: a dinâmica do debate foi fortalecida pelo contexto sociopolítico daqueles anos, que favoreceu a emergência dos movimentos de mulheres e feminista no Brasil, cujas influências foram também sentidas em outras tradições de fé cristã; e o resultado final do debate dependeu mais das questões de gênero e poder do que das discussões técnicas e acadêmicas sobre o acerto histórico do marco inicial do trabalho batista no Brasil. O ineditismo desta pesquisa está em oferecer uma nova perspectiva do debate sobre as origens do trabalho batista no Brasil, a partir do uso da categoria de gênero como instrumento de análise, o que complementará, desse modo, a pesquisa acadêmica já publicada sobre o tema.

Clogging in subsurface-flow treatment wetlands:measurement, modeling and management

This paper reviews the state of the art in measuring, modeling, and managing clogging in subsurface-flow treatment wetlands. Methods for measuring in situ hydraulic conductivity in treatment wetlands are now available, which provide valuable insight into assessing and evaluating the extent of clogging. These results, paired with the information from more traditional approaches (e.g., tracer testing and composition of the clog matter) are being incorporated into the latest treatment wetland models. Recent finite element analysis models can now simulate clogging development in subsurface-flow treatment wetlands with reasonable accuracy. Various management strategies have been developed to extend the life of clogged treatment wetlands, including gravel excavation and/or washing, chemical treatment, and application of earthworms. These strategies are compared and available cost information is reported. © 2012 Elsevier Ltd.

Last Interglacial synthesis of high-latitude temperature: temperature anomalies and associated errors for 4 time slices

The Last Interglacial (LIG, 129-116 thousand of years BP, ka) represents a test bed for climate model feedbacks in warmer-than-present high latitude regions. However, mainly because aligning different palaeoclimatic archives and from different parts of the world is not trivial, a spatio-temporal picture of LIG temperature changes is difficult to obtain. Here, we have selected 47 polar ice core and sub-polar marine sediment records and developed a strategy to align them onto the recent AICC2012 ice core chronology. We provide the first compilation of high-latitude temperature changes across the LIG associated with a coherent temporal framework built between ice core and marine sediment records. Our new data synthesis highlights non-synchronous maximum temperature changes between the two hemispheres with the Southern Ocean and Antarctica records showing an early warming compared to North Atlantic records. We also observe warmer than present-day conditions that occur for a longer time period in southern high latitudes than in northern high latitudes. Finally, the amplitude of temperature changes at high northern latitudes is larger compared to high southern latitude temperature changes recorded at the onset and the demise of the LIG. We have also compiled four data-based time slices with temperature anomalies (compared to present-day conditions) at 115 ka, 120 ka, 125 ka and 130 ka and quantitatively estimated temperature uncertainties that include relative dating errors. This provides an improved benchmark for performing more robust model-data comparison. The surface temperature simulated by two General Circulation Models (CCSM3 and HadCM3) for 130 ka and 125 ka is compared to the corresponding time slice data synthesis. This comparison shows that the models predict warmer than present conditions earlier than documented in the North Atlantic, while neither model is able to produce the reconstructed early Southern Ocean and Antarctic warming. Our results highlight the importance of producing a sequence of time slices rather than one single time slice averaging the LIG climate conditions.

Joan Little

General note: Title and date provided by Bettye Lane.

Joan Little's lawyer, Karen Galloway (Beathea-Shields) speaks at Union Square demonstration for Joan Little

General note: Title and date provided by Bettye Lane.

Wilma Scott Heide at podium at NOW Conference, Washington, D.C.

General note: Title and date provided by Bettye Lane.

Joan Little

General note: Title and date provided by Bettye Lane.

Wilma Scott Heide at NOW conference

General note: Title and date provided by Bettye Lane.

Joan Hachet at the Feminist Newspaper Majority Report conference

General note: Title and date provided by Bettye Lane.

U.S. Representatives to the NGO conference: Jewel Lafontant, Patricia Hutar, Joan Goodin, and Arvonne Fraser

General note: Title and date provided by Bettye Lane.

Joan Mondale at the Democratic National Convention

General note: Title and date provided by Bettye Lane.

Maya Angelou and Coretta Scott King at International Women's Year conference in Houston

General note: Title and date provided by Bettye Lane.