945 resultados para Hitsauksen suurtehomenetelmät, High productive welding processes


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Background. The United States continues to have the highest rates in teenage pregnancy among industrialized nations and approximately 46% of high school students engaged in sex by the time they graduated. Various family adolescent processes (family connectedness, perceived parental beliefs about sex, parent-child communication about sex) have been linked to adolescent sexual behavior. However, the association between family adolescent and adolescent sexual intentions has not often been studied in middle school minority youth.^ Methods. Research hypotheses were tested using a secondary data analysis from a HIV, STI, and pregnancy prevention program for urban middle school students.^ Results. At baseline, 77% of students reported low intentions to engage in vaginal or oral sex within a year and 87% reported they would use a condom if having sex within the next 3 months. After adjusting for gender, age, and race/ethnicity, family connectedness and perceived parental beliefs about sex were significantly associated with vaginal and oral sex intentions. Only perceived parental beliefs was associated with condom use intentions. ^ Conclusions. Family adolescent processes appear to be associated with adolescents’ intentions regarding sex and condom use. Early interventions are needed that take into account the importance of healthy, supportive parent-adolescent relationships and encourage parents to share their beliefs about sex to adolescents before the onset of sexual activity. ^

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Purpose. The focus of maternal role development, historically, has been on the tasks and processes during pregnancy as they relate to postpartum role transition. The purpose of this study was to investigate how women hospitalized with high-risk pregnancy cognitively construct pregnancy and impending motherhood. ^ Design. The study employed a triangulation design using a convergence model with a dominant focused ethnographic approach. ^ Setting. The antepartum units of two tertiary care centers in a large metropolitan city in southeast Texas. ^ Sample. Data saturation was determined with thirteen (13) primigravid women who had been hospitalized more than 72 hours with preterm labor (PTL) or preterm premature rupture of membranes (PPROM) who subsequently delivered seventeen (17) infants which included 4 sets of twins. ^ Methods. Open-ended, semi-structured interviews and field work were used to explore the development of maternal role in this population. After collecting descriptive data, long individual interviews were conducted and the Prenatal Self Evaluation Questionnaire (PSEQ), an instrument to measure prenatal adaptation to pregnancy, was administered. The interview focused on exploring the woman's experiences of pregnancy and impending motherhood while hospitalized. Interview data and field notes were coded and analyzed using qualitative thematic analytic techniques. The PSEQ was scored and the findings of the qualitative data and PSEQ data were compared. ^ Findings. Thematic analysis of the qualitative data provided an understanding of the cognitive process that occurs as the pregnant woman builds a relationship with the fetus. Thematic analysis resulted in a conceptual model with two complementary components that occur throughout the pregnancy: Establishing a Relationship and Dynamic Equilibrium. Establishing a Relationship includes subthemes of: Courting, Building a Connection, and Engagement. Dynamic equilibrium is the balance between expectations and reality and exists regardless of pregnancy complications. The negotiation of this potential imbalance is triggered by uncertainty, loss of autonomy and control, and isolation and is exacerbated by the high-risk pregnancy and subsequent hospitalization. These triggers can serve as obstacles to maternal role development, but may be mediated by external support from friends and family or health care providers. Support from others may come in the form of anticipatory guidance, presence, or activities that promote self-agency. PSEQ scores were similar to previous reports, but due to the small sample, scores were used primarily for comparison to qualitative data. The qualitative findings were congruent with the PSEQ findings in all of the subscales except in the concern for the well-being of the baby. Interview reports included comments demonstrating significant concern for the well-being of the infant, yet the related subscale did not demonstrate such concern. ^ Conclusions. An understanding of the cognitive process involved in establishing a relationship with the developing fetus related to impending motherhood and the importance of dynamic equilibrium can allow healthcare providers and those who interact with pregnant women to support development of the maternal role and anticipate those barriers that may impede that process. Findings from this study identify those triggers and mediators that influence development of the maternal role and suggest potential intervening strategies for those involved in the care of childbearing families. ^

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The intent of this research was to identify the level of risk methanol posed to a fetus during an ethanol co-exposure. This investigation was prompted by the known competitive inhibition properties of ethanol and the developmental toxicity of methanol. Integrated into this research was the practicality necessitated by regulatory processes, namely: does the risk justify the expense of additional research. To this end, the scope and nature of exposures were summarized to illustrate the ubiquity of these chemicals and the potential for dual exposure. Similarly, severity of outcome was evaluated by systematically reviewing the LOAELs, NOAELs, and statistical significance contained in methanol-induced developmental studies. Results. Blood methanol levels corresponding to developmental effects in laboratory studies were found to be substantially higher than the blood methanol levels predicted in high-risk methanol-ethanol exposure scenarios. This indicates that ethanol would not likely exacerbate methanol toxicity to the point of teratogenicity; however, it is important to note that the developmental toxicity of ethanol—an established human teratogen—was not included in the evaluation. Ethanol's contribution as a developmental toxicant rather than merely as an attenuator of methanol toxicity undermines the severity of effects possible from this chemical combination. Therefore further evaluation is needed to assess the developmental toxicities following dual exposures before rendering methanol and ethanol a high-priority mixture.^

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Essential biological processes are governed by organized, dynamic interactions between multiple biomolecular systems. Complexes are thus formed to enable the biological function and get dissembled as the process is completed. Examples of such processes include the translation of the messenger RNA into protein by the ribosome, the folding of proteins by chaperonins or the entry of viruses in host cells. Understanding these fundamental processes by characterizing the molecular mechanisms that enable then, would allow the (better) design of therapies and drugs. Such molecular mechanisms may be revealed trough the structural elucidation of the biomolecular assemblies at the core of these processes. Various experimental techniques may be applied to investigate the molecular architecture of biomolecular assemblies. High-resolution techniques, such as X-ray crystallography, may solve the atomic structure of the system, but are typically constrained to biomolecules of reduced flexibility and dimensions. In particular, X-ray crystallography requires the sample to form a three dimensional (3D) crystal lattice which is technically di‑cult, if not impossible, to obtain, especially for large, dynamic systems. Often these techniques solve the structure of the different constituent components within the assembly, but encounter difficulties when investigating the entire system. On the other hand, imaging techniques, such as cryo-electron microscopy (cryo-EM), are able to depict large systems in near-native environment, without requiring the formation of crystals. The structures solved by cryo-EM cover a wide range of resolutions, from very low level of detail where only the overall shape of the system is visible, to high-resolution that approach, but not yet reach, atomic level of detail. In this dissertation, several modeling methods are introduced to either integrate cryo-EM datasets with structural data from X-ray crystallography, or to directly interpret the cryo-EM reconstruction. Such computational techniques were developed with the goal of creating an atomic model for the cryo-EM data. The low-resolution reconstructions lack the level of detail to permit a direct atomic interpretation, i.e. one cannot reliably locate the atoms or amino-acid residues within the structure obtained by cryo-EM. Thereby one needs to consider additional information, for example, structural data from other sources such as X-ray crystallography, in order to enable such a high-resolution interpretation. Modeling techniques are thus developed to integrate the structural data from the different biophysical sources, examples including the work described in the manuscript I and II of this dissertation. At intermediate and high-resolution, cryo-EM reconstructions depict consistent 3D folds such as tubular features which in general correspond to alpha-helices. Such features can be annotated and later on used to build the atomic model of the system, see manuscript III as alternative. Three manuscripts are presented as part of the PhD dissertation, each introducing a computational technique that facilitates the interpretation of cryo-EM reconstructions. The first manuscript is an application paper that describes a heuristics to generate the atomic model for the protein envelope of the Rift Valley fever virus. The second manuscript introduces the evolutionary tabu search strategies to enable the integration of multiple component atomic structures with the cryo-EM map of their assembly. Finally, the third manuscript develops further the latter technique and apply it to annotate consistent 3D patterns in intermediate-resolution cryo-EM reconstructions. The first manuscript, titled An assembly model for Rift Valley fever virus, was submitted for publication in the Journal of Molecular Biology. The cryo-EM structure of the Rift Valley fever virus was previously solved at 27Å-resolution by Dr. Freiberg and collaborators. Such reconstruction shows the overall shape of the virus envelope, yet the reduced level of detail prevents the direct atomic interpretation. High-resolution structures are not yet available for the entire virus nor for the two different component glycoproteins that form its envelope. However, homology models may be generated for these glycoproteins based on similar structures that are available at atomic resolutions. The manuscript presents the steps required to identify an atomic model of the entire virus envelope, based on the low-resolution cryo-EM map of the envelope and the homology models of the two glycoproteins. Starting with the results of the exhaustive search to place the two glycoproteins, the model is built iterative by running multiple multi-body refinements to hierarchically generate models for the different regions of the envelope. The generated atomic model is supported by prior knowledge regarding virus biology and contains valuable information about the molecular architecture of the system. It provides the basis for further investigations seeking to reveal different processes in which the virus is involved such as assembly or fusion. The second manuscript was recently published in the of Journal of Structural Biology (doi:10.1016/j.jsb.2009.12.028) under the title Evolutionary tabu search strategies for the simultaneous registration of multiple atomic structures in cryo-EM reconstructions. This manuscript introduces the evolutionary tabu search strategies applied to enable a multi-body registration. This technique is a hybrid approach that combines a genetic algorithm with a tabu search strategy to promote the proper exploration of the high-dimensional search space. Similar to the Rift Valley fever virus, it is common that the structure of a large multi-component assembly is available at low-resolution from cryo-EM, while high-resolution structures are solved for the different components but lack for the entire system. Evolutionary tabu search strategies enable the building of an atomic model for the entire system by considering simultaneously the different components. Such registration indirectly introduces spatial constrains as all components need to be placed within the assembly, enabling the proper docked in the low-resolution map of the entire assembly. Along with the method description, the manuscript covers the validation, presenting the benefit of the technique in both synthetic and experimental test cases. Such approach successfully docked multiple components up to resolutions of 40Å. The third manuscript is entitled Evolutionary Bidirectional Expansion for the Annotation of Alpha Helices in Electron Cryo-Microscopy Reconstructions and was submitted for publication in the Journal of Structural Biology. The modeling approach described in this manuscript applies the evolutionary tabu search strategies in combination with the bidirectional expansion to annotate secondary structure elements in intermediate resolution cryo-EM reconstructions. In particular, secondary structure elements such as alpha helices show consistent patterns in cryo-EM data, and are visible as rod-like patterns of high density. The evolutionary tabu search strategy is applied to identify the placement of the different alpha helices, while the bidirectional expansion characterizes their length and curvature. The manuscript presents the validation of the approach at resolutions ranging between 6 and 14Å, a level of detail where alpha helices are visible. Up to resolution of 12 Å, the method measures sensitivities between 70-100% as estimated in experimental test cases, i.e. 70-100% of the alpha-helices were correctly predicted in an automatic manner in the experimental data. The three manuscripts presented in this PhD dissertation cover different computation methods for the integration and interpretation of cryo-EM reconstructions. The methods were developed in the molecular modeling software Sculptor (http://sculptor.biomachina.org) and are available for the scientific community interested in the multi-resolution modeling of cryo-EM data. The work spans a wide range of resolution covering multi-body refinement and registration at low-resolution along with annotation of consistent patterns at high-resolution. Such methods are essential for the modeling of cryo-EM data, and may be applied in other fields where similar spatial problems are encountered, such as medical imaging.

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Changes of glaciers and snow cover in polar regions affect a wide range of physical and ecosystem processes on land and in the adjacent marine environment. In this study, we investigate the potential of 11-day repeat high-resolution satellite image time series from the TerraSAR-X mission to derive glaciological and hydrological parameters on King George Island, Antarctica during the period Oct/25/2010 to Apr/19/2011. The spatial pattern and temporal evolution of snow cover extent on ice-free areas can be monitored using multi-temporal coherence images. SAR coherence is used to map glacier extent of land terminating glaciers with an average accuracy of 25 m. Multi-temporal SAR color composites identify the position of the late summer snow line at about 220 m above sea level. Glacier surface velocities are obtained from intensity feature-tracking. Surface velocities near the calving front of Fourcade Glacier were up to 1.8 ± 0.01 m/d. Using an intercept theorem based on fundamental geometric principles together with differential GPS field measurements, the ice discharge of Fourcade Glacier was estimated to 20700 ± 5500 m**3/d (corresponding to ~19 ± 5 kt/d). The rapidly changing surface conditions on King George Island and the lack of high-resolution digital elevation models for the region remain restrictions for the applicability of SAR data and the precision of derived products.

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Biogenic particle fluxes from highly productive surface waters, boundary scavenging, and hydrothermal activity are the main factors influencing the deposition of radionuclides in the area of the Galapagos microplate, eastern Equatorial Pacific. In order to evaluate the importance of these three processes throughout the last 100 kyr, concentrations of the radionuclides 10Be, 230Th, and 231Pa, and of Mn and Fe were measured at high resolution in sediment samples from two gravity cores KLH 068 and KLH 093. High biological productivity in the surface waters overlying the investigated area has led to 10Be and 231Pa fluxes exceeding production during at least the last 30 kyr and probably the last 100 kyr. However, during periods of high productivity at the up welling centers off Peru and extension of the equatorial high-productivity zone, a relative loss of 10Be and 231Pa may have occurred in these sediment cores because of boundary scavenging. The effects of hydrothermal activity were investigated by comparing the 230Thex concentrations to the Mn/Fe ratios and by comparing the fluxes of 230Th and 10Be which exceed production. The results suggest an enhanced hydrothermal influence during isotope stages 4 and 5 and to a lesser extent during isotope stage 1 in core KLH 093. During isotope stages 2 and 3, the hydrothermal supply of Mn was deposited elsewhere, probably because of changes in current regime or deep water oxygenation. A strong increase of the Mn/Fe ratio at the beginning of climatic stage 1 which is not accompanied by an increase of the 230Thex concentration is interpreted to be an effect of Mn remobilization and reprecipitation in the sediment.

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Colombia es uno de los países denominados megadiversos biológica y culturalmente. Presenta a su interior la mayor extensión de páramos del mundo, los cuales son considerados fábricas de agua del planeta y hábitats de rica diversidad biológica. Por otro lado, la ocupación humana de los páramos colombianos ha generado conflictos entre las normativas ambientales vigentes y los usos productivos del suelo. El presente artículo analiza las alternativas aplicadas ante el conflicto entre autoridades ambientales de áreas protegidas y habitantes de los páramos en Colombia. Se encontraron dos tipos de alternativas: la primera plantea la concesión de servicios ecoturísticos en los parques naturales y la compra de tierras por parte de sociedades de economía mixta. La segunda plantea el abandono de las actividades productivas de los agricultores, mediado por procesos educativos o de cumplimiento de las normas ambientales vigentes, so pena de enfrentar acciones punitivas por parte del Estado. Este trabajo presenta una tercera alternativa, que parte de la crítica a las dos anteriores e incluye diferentes estrategias: planes de manejo comunitario con tiempo y financiación institucional pertinente, aplicación de modelos agroecológicos y rescate de la memoria biocultural y cambios en la estructura agraria.

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Históricamente la captación estadística del empleo agropecuario (y más específicamente del empleo asalariado agropecuario) ha presentado una serie de problemas y limitaciones para las fuentes de datos tradicionales. Los elevados niveles de transitoriedad, estacionalidad, no registro e informalidad han tenido como consecuencia una serie de dificultades en su cuantificación por medio de las fuentes censales y muestrales tradicionales. Los procesos más recientes que atraviesan a esta fracción social (urbanización, acortamiento de ciclos productivos y ocupacionales, disminución de requerimientos de fuerza de trabajo por la mecanización de ciertas cosechas, etc.) parecen haber incrementado dichas dificultades. Trabajos previos realizados demuestran que los censos de población y agropecuarios arrojan diferentes resultados en la cuantificación de los asalariados del sector. Se presenta en este documento un análisis comparado de los resultados obtenidos en Argentina, por el Censo Nacional de Población y Vivienda de 2001 y el Censo Nacional Agropecuario de 2002. El objetivo buscado es realizar una aproximación a las diferentes cifras de asalariados en el agro que arrojan ambos relevamientos en todos los departamentos del país. A su vez, se intentará vincular dichas diferencias con los distintos territorios y distintas estructuras sociales y agrarias, buscando descubrir si permiten aportar a la explicación de aquellos resultados divergentes. Para ello se confeccionó una base de datos del total del país, desagregada a nivel provincial y departamental (máximo nivel de desagregación permitida por las fuentes publicadas) de la cantidad total de asalariados agropecuarios y diversos indicadores de la estructura social y agraria (cantidad de explotaciones pobres, niveles de urbanización, distribución de la tierra, etc.).

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High-frequency data collected continuously over a multiyear time frame are required for investigating the various agents that drive ecological and hydrodynamic processes in estuaries. Here, we present water quality and current in-situ observations from a fixed monitoring station operating from 2008 to 2014 in the lower Guadiana Estuary, southern Portugal (37°11.30' N, 7°24.67' W). The data were recorded by a multi-parametric probe providing hourly records (temperature, salinity, chlorophyll, dissolved oxygen, turbidity, and pH) at a water depth of ~1 m, and by a bottom-mounted acoustic Doppler current profiler measuring the pressure, near-bottom temperature, and flow velocity through the water column every 15 min. The time-series data, in particular the probe ones, present substantial gaps arising from equipment failure and maintenance, which are ineluctable with this type of observations in harsh environments. However, prolonged (months-long) periods of multi-parametric observations during contrasted external forcing conditions are available. The raw data are reported together with flags indicating the quality status of each record. River discharge data from two hydrographic stations located near the estuary head are also provided to support data analysis and interpretation.

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Colombia es uno de los países denominados megadiversos biológica y culturalmente. Presenta a su interior la mayor extensión de páramos del mundo, los cuales son considerados fábricas de agua del planeta y hábitats de rica diversidad biológica. Por otro lado, la ocupación humana de los páramos colombianos ha generado conflictos entre las normativas ambientales vigentes y los usos productivos del suelo. El presente artículo analiza las alternativas aplicadas ante el conflicto entre autoridades ambientales de áreas protegidas y habitantes de los páramos en Colombia. Se encontraron dos tipos de alternativas: la primera plantea la concesión de servicios ecoturísticos en los parques naturales y la compra de tierras por parte de sociedades de economía mixta. La segunda plantea el abandono de las actividades productivas de los agricultores, mediado por procesos educativos o de cumplimiento de las normas ambientales vigentes, so pena de enfrentar acciones punitivas por parte del Estado. Este trabajo presenta una tercera alternativa, que parte de la crítica a las dos anteriores e incluye diferentes estrategias: planes de manejo comunitario con tiempo y financiación institucional pertinente, aplicación de modelos agroecológicos y rescate de la memoria biocultural y cambios en la estructura agraria.

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Colombia es uno de los países denominados megadiversos biológica y culturalmente. Presenta a su interior la mayor extensión de páramos del mundo, los cuales son considerados fábricas de agua del planeta y hábitats de rica diversidad biológica. Por otro lado, la ocupación humana de los páramos colombianos ha generado conflictos entre las normativas ambientales vigentes y los usos productivos del suelo. El presente artículo analiza las alternativas aplicadas ante el conflicto entre autoridades ambientales de áreas protegidas y habitantes de los páramos en Colombia. Se encontraron dos tipos de alternativas: la primera plantea la concesión de servicios ecoturísticos en los parques naturales y la compra de tierras por parte de sociedades de economía mixta. La segunda plantea el abandono de las actividades productivas de los agricultores, mediado por procesos educativos o de cumplimiento de las normas ambientales vigentes, so pena de enfrentar acciones punitivas por parte del Estado. Este trabajo presenta una tercera alternativa, que parte de la crítica a las dos anteriores e incluye diferentes estrategias: planes de manejo comunitario con tiempo y financiación institucional pertinente, aplicación de modelos agroecológicos y rescate de la memoria biocultural y cambios en la estructura agraria.

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Históricamente la captación estadística del empleo agropecuario (y más específicamente del empleo asalariado agropecuario) ha presentado una serie de problemas y limitaciones para las fuentes de datos tradicionales. Los elevados niveles de transitoriedad, estacionalidad, no registro e informalidad han tenido como consecuencia una serie de dificultades en su cuantificación por medio de las fuentes censales y muestrales tradicionales. Los procesos más recientes que atraviesan a esta fracción social (urbanización, acortamiento de ciclos productivos y ocupacionales, disminución de requerimientos de fuerza de trabajo por la mecanización de ciertas cosechas, etc.) parecen haber incrementado dichas dificultades. Trabajos previos realizados demuestran que los censos de población y agropecuarios arrojan diferentes resultados en la cuantificación de los asalariados del sector. Se presenta en este documento un análisis comparado de los resultados obtenidos en Argentina, por el Censo Nacional de Población y Vivienda de 2001 y el Censo Nacional Agropecuario de 2002. El objetivo buscado es realizar una aproximación a las diferentes cifras de asalariados en el agro que arrojan ambos relevamientos en todos los departamentos del país. A su vez, se intentará vincular dichas diferencias con los distintos territorios y distintas estructuras sociales y agrarias, buscando descubrir si permiten aportar a la explicación de aquellos resultados divergentes. Para ello se confeccionó una base de datos del total del país, desagregada a nivel provincial y departamental (máximo nivel de desagregación permitida por las fuentes publicadas) de la cantidad total de asalariados agropecuarios y diversos indicadores de la estructura social y agraria (cantidad de explotaciones pobres, niveles de urbanización, distribución de la tierra, etc.).

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The outer western Crimean shelf of the Black Sea is a natural laboratory to investigate effects of stable oxic versus varying hypoxic conditions on seafloor biogeochemical processes and benthic community structure. Bottom-water oxygen concentrations ranged from normoxic (175 µmol O2/L) and hypoxic (< 63 µmol O2/L) or even anoxic/sulfidic conditions within a few kilometers' distance. Variations in oxygen concentrations between 160 and 10 µmol/L even occurred within hours close to the chemocline at 134 m water depth. Total oxygen uptake, including diffusive as well as fauna-mediated oxygen consumption, decreased from 15 mmol/m**2/d on average in the oxic zone, to 7 mmol/m**2/d on average in the hypoxic zone, correlating with changes in macrobenthos composition. Benthic diffusive oxygen uptake rates, comprising respiration of microorganisms and small meiofauna, were similar in oxic and hypoxic zones (on average 4.5 mmol/m**2/d), but declined to 1.3 mmol/m**2/d in bottom waters with oxygen concentrations below 20 µmol/L. Measurements and modeling of porewater profiles indicated that reoxidation of reduced compounds played only a minor role in diffusive oxygen uptake under the different oxygen conditions, leaving the major fraction to aerobic degradation of organic carbon. Remineralization efficiency decreased from nearly 100 % in the oxic zone, to 50 % in the oxic-hypoxic zone, to 10 % in the hypoxic-anoxic zone. Overall, the faunal remineralization rate was more important, but also more influenced by fluctuating oxygen concentrations, than microbial and geochemical oxidation processes.

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Históricamente la captación estadística del empleo agropecuario (y más específicamente del empleo asalariado agropecuario) ha presentado una serie de problemas y limitaciones para las fuentes de datos tradicionales. Los elevados niveles de transitoriedad, estacionalidad, no registro e informalidad han tenido como consecuencia una serie de dificultades en su cuantificación por medio de las fuentes censales y muestrales tradicionales. Los procesos más recientes que atraviesan a esta fracción social (urbanización, acortamiento de ciclos productivos y ocupacionales, disminución de requerimientos de fuerza de trabajo por la mecanización de ciertas cosechas, etc.) parecen haber incrementado dichas dificultades. Trabajos previos realizados demuestran que los censos de población y agropecuarios arrojan diferentes resultados en la cuantificación de los asalariados del sector. Se presenta en este documento un análisis comparado de los resultados obtenidos en Argentina, por el Censo Nacional de Población y Vivienda de 2001 y el Censo Nacional Agropecuario de 2002. El objetivo buscado es realizar una aproximación a las diferentes cifras de asalariados en el agro que arrojan ambos relevamientos en todos los departamentos del país. A su vez, se intentará vincular dichas diferencias con los distintos territorios y distintas estructuras sociales y agrarias, buscando descubrir si permiten aportar a la explicación de aquellos resultados divergentes. Para ello se confeccionó una base de datos del total del país, desagregada a nivel provincial y departamental (máximo nivel de desagregación permitida por las fuentes publicadas) de la cantidad total de asalariados agropecuarios y diversos indicadores de la estructura social y agraria (cantidad de explotaciones pobres, niveles de urbanización, distribución de la tierra, etc.).

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Colombia es uno de los países denominados megadiversos biológica y culturalmente. Presenta a su interior la mayor extensión de páramos del mundo, los cuales son considerados fábricas de agua del planeta y hábitats de rica diversidad biológica. Por otro lado, la ocupación humana de los páramos colombianos ha generado conflictos entre las normativas ambientales vigentes y los usos productivos del suelo. El presente artículo analiza las alternativas aplicadas ante el conflicto entre autoridades ambientales de áreas protegidas y habitantes de los páramos en Colombia. Se encontraron dos tipos de alternativas: la primera plantea la concesión de servicios ecoturísticos en los parques naturales y la compra de tierras por parte de sociedades de economía mixta. La segunda plantea el abandono de las actividades productivas de los agricultores, mediado por procesos educativos o de cumplimiento de las normas ambientales vigentes, so pena de enfrentar acciones punitivas por parte del Estado. Este trabajo presenta una tercera alternativa, que parte de la crítica a las dos anteriores e incluye diferentes estrategias: planes de manejo comunitario con tiempo y financiación institucional pertinente, aplicación de modelos agroecológicos y rescate de la memoria biocultural y cambios en la estructura agraria.