Scrambling of the actin I gene in two Oxytricha species.

The DNA in a germ-line nucleus (a micronucleus) undergoes extensive processing when it develops into a somatic nucleus (a macronucleus) after cell mating in hypotrichous ciliates. Processing includes destruction of a large amount of spacer DNA between genes and excision of gene-sized molecules from chromosomes. Before processing, micronuclear genes are interrupted by numerous noncoding segments called internal eliminated sequences (IESs). The IESs are excised and destroyed, and the retained macro-nuclear-destined sequences (MDSs) are spliced. MDSs in some micronuclear genes are not in proper order and must be reordered during processing to create functional gene-sized molecules for the macronucleus. Here we report that the micronuclear actin I gene in Oxytricha trifallax WR consists of 10 MDSs and 9 IESs compared to the previously reported 9 MDSs and 8 IESs in the micronuclear actin I gene of Oxytricha nova. The MDSs in the actin I gene are scrambled in a similar pattern in the two species, but the positions of MDS-IES junctions are shifted by up to 14 bp for scrambled and 138 bp for the nonscrambled MDSs. The shifts in MDS-IES junctions create differences in the repeat sequences that are believed to guide MDS splicing. Also, the sizes and sequences of IESs in the micronuclear actin I genes are different in the two Oxytricha species. These observations give insight about the possible origins of IES insertion and MDS scrambling in evolution and show the extraordinary malleability of the germ-line DNA in hypotrichs.

The evolution and masses of the neutron star and donor star in the high mass X-ray binary OAO 1657−415

We report near-infrared radial velocity (RV) measurements of the recently identified donor star in the high mass X-ray binary (HMXB) system OAO 1657−415 obtained in the H band using ISAAC on the Very Large Telescope. Cross-correlation methods were employed to construct a RV curve with a semi-amplitude of 22.1 ± 3.5 km s−1. Combined with other measured parameters of this system it provides a dynamically determined neutron star (NS) mass of 1.42 ± 0.26 M⊙ and a mass of 14.3 ± 0.8 M⊙ for the Ofpe/WN9 highly evolved donor star. OAO 1657−415 is an eclipsing HMXB pulsar with the largest eccentricity and orbital period of any within its class. Of the 10 known eclipsing X-ray binary pulsars OAO 1657−415 becomes the ninth with a dynamically determined NS mass solution and only the second in an eccentric system. Furthermore, the donor star in OAO 1657−415 is much more highly evolved than the majority of the supergiant donors in other HMXBs, joining a small but growing list of HMXBs donors with extensive hydrogen depleted atmospheres. Considering the evolutionary development of OAO 1657−415, we have estimated the binding energy of the envelope of the mass donor and find that there is insufficient energy for the removal of the donor’s envelope via spiral-in, ruling out a common envelope evolutionary scenario. With its non-zero eccentricity and relatively large orbital period the identification of a definitive evolutionary pathway for OAO 1657−415 remains problematic, we conclude by proposing two scenarios which may account for OAO 1657−415 current orbital configuration.

The syntheses and characterization of nickel complexes; investigation of Ni(dppp)2 and [NiX(dppp)n]x, potentially catalytically active nickel(0)/(I) species for cross-coupling reactions

This thesis describes an investigation in which we compare Ni(0), Ni(I) and Ni(II) complexes containing 1,3-bis(diphenylphosphino)propane (dppp) as a phosphine ligand for their abilities to effect three types of cross-coupling reactions: Buchwald-Hartwig Amination, Heck-Mizoroki, and Suzuki-Miyaura cross-coupling reactions with different types of substrates. The Ni(0) complex Ni(dppp)2 is known and we have synthesized it via a new procedure involving zinc reduction of the known NiCl2(dppp) in the presence of an excess of dppp. The Ni(0) complex was characterized by NMR spectroscopy and X-ray crystallography. Since Ni(I) complexes of dppp seem unknown, we have synthesized what at this stage appear to be NiXdpppn/[NiX(dppp)n]x (X = Cl, Br, I; n = 1,2, x = 1, 2) by comproportionation of molar equivalents of Ni(dppp)2 and NiX2dppp, X= Cl, Br, I.

UM Women's Track Team, 1980

Front Row: Julie Clifford, Dawn Woodruff, Dana Loesch, Sheila Mayberry, Lisa Larsen, Melanie Weaver, Brenda Kazinec

Middle Row: DeDe Key, Kathy Kampen, Tina Smith, Carolyn Clymer, Catherine Sharpe, Sue Frederick, Sherrie King, Maureen Miner

Back Row: asst. coach Sheila Shetter, Debbie Williams, Lynn Fudala, Joanna Bullard, Renee Turner, Penny Neer, Lorrie Thornton, head coach Ken "Red" Simmons

Jan Sobieski i jego wiek.

Written on the occasion of the second centennial of the liberation of Vienna by Sobieski.

Luther's reply to King Henry VIII, now first Englished after the lapse of four centuries,

Mode of access: Internet.

The historical romances of Louisa Mühlbach.

v. 1. Napoleon and the queen of Prussia. -- v. 2. The empress Josephine. -- v. 3. Napoleon and Blücher. -- v. 4. Queen Hortense. -- v. 5. Marie Antoinette and her son. -- v. 6. Prince Eugene and his times. -- v. 7. The daughter of an empress. -- v. 8. Joseph the Second and his court. -- v. 9. Frederick the Great and his court. -- v. 10. Frederick the Great and his family. -- v. 11. Berlin & Sans Souci. -- v. 12. Goethe and Schiller. -- v. 13. The merchant of Berlin. -- v. 14. Louisa of Prussia and her times. -- v. 15. Old Fritz and the new era. -- v. 16. Andreas Hofer. -- v. 17. Mohammed Ali and his house. -- v. 18. Henry the Eighth and his court. -- v. 19. The youth of the Great Elector. -- v. 20. The reign of the Great Elector.

Bèohmens Antheil an den Kèampfen Kaiser Friedrich I in Italien.

Acquisition made accessible thanks to a 2015-2017 grant from the Council on Libraries and Information Resources.

The age of Alfred,

Mode of access: Internet.

The Weekly notes

Editor: 1895-1935, Sir Frederick Pollock, bart

Legal maxims, with observations and cases : in two parts, Part I. One hundred maxims, with observations and cases. Part II. Eight hundred maxims, with translations /

Mode of access: Internet.

Life of George Stubbs, R.A. /

Includes appendices.

The life of Louis XI, the rebel dauphin and the statesman king, from his original letters and other documents;

"Printed in Great Britain."

Reconnaissance of Red Rock District, Cove Mesa, and Kinusta (tree) Mesa, Arizona /

Red Rock district, Cove mesa, and Kinusta mesa lie in southern Carrizo Mountain region of Apache County, Arizona, 25 miles south of the Utah State line. The Red Rock district lies along the Arizona-New Mexico line, and the two mesas are approximately 15 miles to the west (Plate I). All of these areas are within the boundaries of Navajo reservations in Arizona and New Mexico.

The visit of His Royal Highness the Prince of Wales to America, reprinted from the Lower Canada Journal of Education,

The first half originally written in French and tr. into English by Joseph Lenoir.