Linear low-density polyethylene colored with a nanoclay-based pigment: Morphology and mechanical, thermal, and colorimetric properties

In this study, a novel kind of hybrid pigment based on nanoclays and dyes was synthesized and characterized. These nanoclay-based pigments (NCPs) were prepared at the laboratory with sodium montmorillonite nanoclay (NC) and methylene blue (MB). The cation-exchange capacity of NC exchanged with MB was varied to obtain a wide color gamut. The synthesized nanopigments were thoroughly characterized. The NCPs were melt-mixed with linear low-density polyethylene (PE) with an internal mixer. Furthermore, samples with conventional colorants were prepared in the same way. Then, the properties (mechanical, thermal, and colorimetric) of the mixtures were assessed. The PE–NCP samples developed better color properties than those containing conventional colorants and used as references, and their other properties were maintained or improved, even at lower contents of dye compared to that with the conventional colorants.

A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones

Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted clones obviating the need for selection and outgrowth of drug resistant clones. Importantly, the use of a promoter-less, ATG-less construct greatly facilitates the recovery of correctly targeted cells. Using this method we report successful gene targeting in up to 94% of recovered human somatic cell clones. We create functional EYFP-tagged knockin clones in both transformed and non-transformed human somatic cell lines providing a valuable tool for mammalian cell biology. We further demonstrate the use of this technology to create gene knockouts. Using this generally applicable strategy we can recover gene targeted clones within approximately one month from DNA construct delivery to obtaining targeted monoclonal cell lines.

Beef versus wool production in Australia /

Cover title.

Letters addressed to the Right Honourable the Earl of Clancarty, president of the Board of trade ... on the inexpediency of permitting the importation of cotton wool from the United States, during the present war.

Mode of access: Internet.

Preventing health hazards from exposure to benzidine cogener dyes.

Mode of access: Internet.

Mackenzie's ten thousand receipts : in all the useful and domestic arts : constituting a complete and practical library.

Includes index.

The new Columbian White House cookery : containing toilet, medical, and cooking receipts, comprehending economical and practical information pertaining to the successful management of the home /

Includes samples of menus; household hints; some recipes include wine or liquor as an ingredient. Sample recipes: Chicken cream soup, Eels a la tartare, Lemon brandy (for cakes and puddings), Spiced nutmeg melon.

Fine wool sheep husbandry.

Mode of access: Internet.

Merchandise rates, including live stock, wool, etc.

At head of title: Victorian Railways.

The photodynamic action of dyes on the eggs of the sea urchin, Lytechinus variegatus,

Descriptive letterpress on versos facing the plates.

The manufacture of leather /

Disbound Original Held in Oak Street Library Facility.

Industrial hygiene surveys of occupational exposure to mineral wool /

Mode of access: Internet.

The structure of the wool fibre, in its relation to the use of wool for technical purposes;

Glossary.

Wool-gathering /

Mode of access: Internet.

Situation and outlook yearbook.

Title from cover.