848 resultados para Development processes


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The document revisits, broadens and updates the discussions contained in ECLAC documents Input for the preparation of a regional agenda for the Programme of Action of the International Conference on Population and Development: towards 2014 and beyond (LC/L.3219(CEP.2010/4)), of 2010, and Reflections on the population and development agenda for Latin America and the Caribbean beyond 2014 (LC/L.3481(CEP.2/5)), of 2012..

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IEEE 1451 is Standard to connect transduscers through a communication network. This article describes a supervisory system implementaion according to IEEE 1451 Standard using Java. This software, named NCAP, may be used in several industrial applications, besides making feasible to obtain TEDS as a report or by Internet. NCAP communicates with transduscer module through RS232 Interface and was testes in industrial automation processes. © 2009 IEEE.

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Metagenomics has been widely employed for discovery of new enzymes and pathways to conversion of lignocellulosic biomass to fuels and chemicals. In this context, the present study reports the isolation, recombinant expression, biochemical and structural characterization of a novel endoxylanase family GH10 (SCXyl) identified from sugarcane soil metagenome. The recombinant SCXyl was highly active against xylan from beechwood and showed optimal enzyme activity at pH 6,0 and 45°C. The crystal structure was solved at 2.75 Å resolution, revealing the classical (β/α)8-barrel fold with a conserved active-site pocket and an inherent flexibility of the Trp281-Arg291 loop that can adopt distinct conformational states depending on substrate binding. The capillary electrophoresis analysis of degradation products evidenced that the enzyme displays unusual capacity to degrade small xylooligosaccharides, such as xylotriose, which is consistent to the hydrophobic contacts at the +1 subsite and low-binding energies of subsites that are distant from the site of hydrolysis. The main reaction products from xylan polymers and phosphoric acid-pretreated sugarcane bagasse (PASB) were xylooligosaccharides, but, after a longer incubation time, xylobiose and xylose were also formed. Moreover, the use of SCXyl as pre-treatment step of PASB, prior to the addition of commercial cellulolytic cocktail, significantly enhanced the saccharification process. All these characteristics demonstrate the advantageous application of this enzyme in several biotechnological processes in food and feed industry and also in the enzymatic pretreatment of biomass for feedstock and ethanol production. © 2013 Alvarez et al.