Efeito da adição de probiótico e/ou prebiótico em dietas de leitões desmamados sobre o desempenho, incidência de diarréia e contagem de coliformes totais

In the Experiment 1, 50 pigs weaned at the 21 days were fed with either of five diets: T1 - basal diet; T2 - basal diet + antibiotic; T3 - basal diet + probiotic; T4 - basal diet + prebiotic; T5 - basal diet + simbiotic. The variables studied were body weight, feed intake and feed conversion in the Phase 1 (21 to 43 days), Phase 2 (44 to 57 days), Phase 3 (58 to 70 days) and Total Phase (21 to 70 days). During the performance experiment, a fecal survey score was conducted to verify diarrhea incidence. In the Experiment 2, 44 pigs weaned at 21 days were fed with the same diets of Experiment 1. Pigs were slaughtered at three differents ages (at weaning, at seven and 14 days after weaning). A segment of the small intestine was collected for analisys of total coliformes. The results showed that the best performance was obtained with the utilization of prebiotic and simbiotic. There were not differences in relation to diarrhea incidence among the treatments studied. The addition of probiotic and/or prebiotic in the diet prevented increase of colonization by pathogenic bacterias from seven to 14 days after weaning.

Estimativa do padrão de preferência térmica de matrizes pesadas (frango de corte)

Female broiler breeder productivity is based on the principles of thermal comfort that are directly related with the microclimate inside the housing. This research had the objective of monitoring the behavior of female broiler breeders, using the technology of radio-frequency, injectable transponders and readers in different existing microclimates inside a small scale distorted housing model. Eight birds with electronic identification were used. Three readers were used, in three different points inside the model: on the floor of the nest, in the passage besides the lateral wall and below the water facility. Dry bulb (DBT), wet bulb (WBT) and black globe (BGT) temperature were measured continuously. The results point out a distinct behavioral pattern of the birds regarding the environment exposition during the experiment. Three probabilistic models of behavior were developed from the recorded data: probabilistic model for the passage use: FP = 1.10 - 0.244 ln(DBT), probabilistic model for the water facility use: FB = 0.398 + 0.00866(DBT), and probabilistic model for the nest use: FN = 2.22 - 0.272 DBT + 0,011 DBT 2 - 0.000144 DBT 3.

Acurácia do teste de escada utilizando o consumo máximo de oxigênio como padrão-ouro

Objective: To determine the accuracy of the variables related to the fixed-height stair-climbing test (SCT) using maximal oxygen uptake (V̇O 2 max) as the gold standard. Methods: The SCT was performed on a staircase consisting of 6 flights (72 steps; 12.16 m total height), with verbal encouragement, in 51 patients. Stair-climbing time was measured, the variables 'work' and 'power' also being calculated. The V̇O2 max was measured using ergospirometry according to the Balke protocol. We calculated the Pearson linear correlation (r), as well as the values of p, between the SCT variables and V̇O2 max. To determine accuracy, the V̇O 2 max cut-off point was set at 25 mL/kg/min, and individuals were classified as normal or altered. The cut-off points for the SCT variables were determined using the receiver operating characteristic curve. The Kappa statistic (k) was used in order to assess concordance. Results: The following values were obtained for the variable 'time': cut-off point = 40 s; mean = 41 ± 15.5 s; r = -0.707; p < 0.005; specificity = 89%; sensibility = 83%; accuracy = 86%; and k = 0.724. For 'power', the values obtained were as follows: cut-off point = 200 w; mean = 222.3 ± 95.2 w; r = 0.515; p < 0.005; specificity = 67%; sensibility= 75%; accuracy = 71%; and k = 0.414. Since the correlation between the variable 'work' and V̇O2 max was not significant, that variable was discarded. Conclusion: Of the SCT variables tested, using V̇O2 max as the gold standard, the variable 'time' was the most accurate.

Atividades séricas da aspartato aminotransferase, creatina quinase e lactato desidrogenase de eqüinos submetidos ao teste padrão de exercício progressive em esteira

The aim of this study was to determine the serum activities of enzymes aspartate aminotransferase, creatine kinase and lactate dehydrogenase in Arabian horses submitted to exercise on high-speed equine treadmill. Eleven mature Arabian horse were training and submitted to Standard Incremental Exercise Test on high-speed equine treadmill. Venous blood samples were taken before exercise, immediately and 30 min, 60min, 3h, 6h, 24h, 3 days and 5 days after exercise. The serum activity aspartate aminotransferase, creatine kinase and lactate dehydrogenase were determined. The serum activies of AST, CK and LDH increase immediately and returned to baseline value 30 minutes after exercise. The AST enzyme activity increased at 12 hours and 24 hours, CK at 3 hours and 6 hours, and LDH at 24 hours after Standard Incremental Exercise Test.

Aplicação da espirometria durante teste padrão de exercício progressivo em esteira para avaliação da troca gasosa respiratória de equinos da raça árabe

The current study evaluated equine gas exchange responses through spirometry, by measuring oxygen uptake (VO2), carbon dioxide production (VCO2), respiratory exchange ratio (R) and maximum oxygen uptake (VO2max) of Arabian horses during a standard incremental exercise test performed on a high-speed treadmill. Six clinically healthy Arabian horses were submitted to a standard incremental exercise test, performed on a high-speed treadmill at a 6% slope, and initial speed of 1,8 m.s-1 for 5 minutes, then 4,0 m.s-1 for 3 minutes, 6,0 m.s-1 for 2 minutes and 8,0 m.s-1, 9,0 m.s-1, 10,0 m.s-1 and 11,0 m.s-1 for one minute for each of these speed. The end of the exercise test was defined as the point in which the horse was no longer able to keep pace with the treadmill. Gas exchange was measured through respiratory analyses horse mask on the last 10 seconds at the end of each speed transition and at 1, 2 and 3 minutes after the end of the exercise, defined as experiment moments. During exercise, it was noticed an increase on both VO2 and VCO2 with linear relationship between exercise and speed. Arabian horses VO2max was 114,9 mL.kg-1.min-1. The respiratory exchange ratio increased over 1,0 by the speed of 9,0 m.s-1, indicating the preponderance of the anaerobic metabolism, and remains at a high level on the post-exercise period.

Relevãncia do padrão de remodelamento ventricular no modelo de infarto do miocárdio em ratos

Background: The relevance of the remodeling pattern in the model of infarcted rats is not known. Objective: To analyze the presence of different patterns of remodeling in this model and its functional implications. Methods: Infarcted rats (n=47) have been divided according to the geometry pattern, analyzed by echocardiogram: normal (normal mass index and normal relative thickness), concentric remodeling (normal mass index and increased relative thickness), concentric hypertrophy (increased mass index and increased relative thickness) and eccentric hypertrophy (increased mass index and normal relative thickness). Data are median and interquartile range. Results: Infarcted rats showed only two of the four geometric patterns: normal pattern (15%) and eccentric hypertrophy - EH (85%). Groups of normal pattern and EH showed no differences in the values of fractional area change (Normal = 32.1-28.8 to 50.7; EH = 31.3-26.5 to 36.7; p = 0.343). Out of the infarcted animals, 34 (74%) had systolic dysfunction, detected by fractional area change. Considering these two geometry patterns, 77% of animals with eccentric hypertrophy and 57% with normal geometry presented systolic dysfunction (p=0.355). The relative wall thickness, the geometric patterns and the body mass index were not predictors of ventricular dysfunction (p>0.05). On the other hand, infarct size was a predictive factor for ventricular dysfunction in univariate analysis (p<0.001) and multivariate analysis (p = 0.004). Conclusion: Rats that underwent coronary occlusion showed two different patterns of remodeling, which do not constitute a predictor of ventricular dysfunction.

Bactérias e resposta inflamatória no útero de cadelas, após inseminação artificial

The present study aimed to obtain information about the uterine inflammatory response (number of polymorphonuclear neutrophilic granulocytes - PMNs) in bitches after artificial insemination (AI) and identify the uterine microflora present after the following treatments: insemination using semen with extender (n=6), insemination with fresh semen (n=6) and no inseminated (n=6). The percentage of PMNs on the endometrial surface and within histological sections was evaluated together with the presence of aerobic bacteria in the uterine lumen. For endometrial cytology, there was no significative difference on the number of inflammatory cells between bitches not inseminated (3.05 ± 1.74 PMNs) and those inseminated with fresh semen (3.55 ± 1.51 PMNs); There was a significative difference in both groups compared to the inseminated with semen plus extender (7.80 ± 1.67 PMNs) (p<0.05). Histology showed that there was no significative difference on the number of inflammatory cells between bitches not inseminated (87.72 ± 35.2 PMNs) and those inseminated with fresh semen (122.97 ± 43.31 PMNs); however, it was observed differences in both groups compared to those inseminated with semen plus extender (171.94 ± 42.74 PMNs) (p<0.05). Eight animals, randomly distributed in the groups, showed the presence of Staphylococcus sp and Proteus sp., in the microbiological exam. The extender for semen, with Tris, is a potent inducer of uterine inflammation, and positive uterine cultures may be obtained during estrus without inflammation or uterine infection.

Epidemiologia do Padrão Face Longa em escolares do Ensino Fundamental do município de Bauru - SP

OBJECTIVE: This study aimed to classify and determine the prevalence of individuals with vertical alteration of facial relationships, according to the severity of discrepancy, especially individuals with long face pattern. METHODS: The sample was composed of 5,020 individuals of Brazilian nationality, of both genders, aged 10 years to 16 years and 11 months, attending fundamental schools at the city of Bauru-SP. Examination of facial morphology comprised direct observation of the face in frontal and lateral views, always with the lips at rest, aiming to identify the individuals presenting vertical alteration of facial relationships. After identification, these individuals were scored, according to severity, into three subtypes, namely mild, moderate and severe. The prevalence of individuals with long face pattern considered only the individuals scored as subtypes moderate and severe. RESULTS: There was prevalence of 34.94% of vertical alteration of facial relationships and 14.06% of long face pattern. CONCLUSIONS: The results obtained in this study revealed that the prevalence of vertical alteration of facial relationships and long face pattern was higher than reported in the literature.

Associação do padrão de ingestão lípidica com a qualidade da dieta, resistência insulínica e homocisteinemia em adultos

Objectives: To evaluate the association between the consumption of different dietary fats with the quality of the diet, insulin resistance, and hyperhomocysteinemia in adults. Methods: Cross-sectional study conducted with 624 overweight subjects (73.7% females). Assessments of food intake (24h food recall and health eating index-HEI), anthropometry, and biochemical assays of fasting glucose, insulin (HOMA-IR and β calculus) and homocysteinemia were performed. Results: The low quality of diet was associated with the vegetable oil at 3rd quintile (≥1.5-2.0 servings) showed risk 2.9 times and cholesterol at quintiles 2nd, 3rd, and 4th was 2.0 times. HOMA-IR was higher at 5th quintile of saturated fat (≥10,7% - total caloric value) with risk of 60% and hyperhomocysteinemia the vegetable oil at 3rd quintile (>1.5-2.0 servings) with risk of 12.0 times and 5th (≥3.5 servings) 7.1 times. However, significance disappeared when adjusted for anthropometric variables. Conclusion: Dietary fats were associated with the harm diet quality, insulin resistance, and hyperhomocysteinemia. However, associations are dependant of demographic variables, dietetic, and nutritional state. © 2011 CELOM.

Diferenças sazonais no padrão endócrino e ovariano do ciclo estral de novilhas bos taurus indicus (Nelore)

Estrous cycle of eight Nelore heifers were evaluated during different seasons of the year (autumn n=11; winter n=8; spring n=9 and summer n=9) with daily count and measurement of follicles ≥3mm, blood was collected every 12h for LH and progesterone (P4), and after estrous every 3h for LH peak. Five ovariectomized heifers were injected with 17β-estradiol (2μg/kg) every season and blood samples collected every 3h (for 30h) thereafter for LH quantification. The monthly percent body weight difference (Δ%) did not vary among seasons. P4 concentration was higher (p<0.01) and follicle number lower during autumn and summer compared to winter and spring. During winter there were more estrous cycles with three and during summer only cycles with two follicular waves (p<0.01). As LH secretion did not vary despite P4 concentration and as there was negative correlation between higher P4 values and daily percentile variation of photoperiod (Δ%, p<0.01; r= -0.45) it is possible to suppose that there is seasonal variation on luteal cell sensitivity to LH. In the ovariectomized Nelore heifers, the LH basal concentration (without estradiol stimulus, p=0.02) and the LH response to estradiol (p<0.01) were lower during summer, leading to the hypothesis that there is seasonal variation of hypothalamic sensitivity to estradiol. According to the present experiment there are suggestions of seasonal reproduction in Nelore heifers.

Acúmulo de biomassa em variedades de cana-de-açúcar inoculadas com diferentes estirpes de bactérias diazotróficas

The objective of this study was to evaluate the contribution of inoculation with diazotrophs, applied both individually and in combination, in commercial varieties of sugarcane. The experiment was carried out at the experimental grounds of Embrapa Agrobiologia, in Seropédica, Rio de Janeiro, Brazil. The experimental design was of randomised blocks, with sub-divided plots and six replications. Six varieties of sugarcane with six treatments were used: control, control with nitrogen, inoculation of the individual strains: BR 11512, BR 11724 and BR 11411, in addition to inoculation with a cocktail consisting of five strains of diazotrophs. Differences were observed between varieties and between treatments, and an interaction observed between the treatments and varieties. For all the parameters evaluated, no response was observed in the sugarcane varieties RB855536 and RB92606. In the variety RB918639, the only difference was in the accumulation of green biomass. No difference was observed between the treatments for total nitrogen accumulation. The most responsive variety to inoculation was RB867515, differing in the accumulation of fresh and dry biomass. This study showed that inoculation promotes the accumulation of biomass, the contribution being different for the varieties and strains of bacteria, suggesting an interaction between the factors studied. The variety RB867515 is promising for use in studies of inoculation with diazotrophs.

Atividade proteolìtica de bactérias psicrotróficas em leites estocados em diferentes temperaturas

The widespread use of cooling technology as a tool for maintaining the microbiological and organoleptic characteristics of milk, Without effective hygienic practices throughout the milk production, caused the psychrotrophic micro-organisms to become major deteriorative bacteria in the dairy industry. The present study aimed to assess how the proteolytic psychrotrophic metabolism responds to different milk storage temperatures, drawing a parallel among psychrotrophic growth, amount of micro-organisms capable of breaking down proteins and amount of GMP (glycomacropeptide) released. Milk samples were collected directly from the cooling tank and subjected to count of psychrotrophs, count of proteolytic micro-organisms and the concentration of GMP at different storage times and temperatures. There was no linearity between the microbiological parameters and the GMP measured, according to the time/temperature binomial. The highest concentration of GMP (5.07 mg/mL) was measured in the binomial 8°C/24h (T8/ M24). The data make clear the need for further studies about questions involving the psychrotrophic microbiota in order to clarify extremely important basic questions about their metabolism.

Avaliação dos indicadores nutricionais e da composição corporal em hepatopatas crônicos e a relação com a etiologia e gravidade da doença

Pós-graduação em Bases Gerais da Cirurgia - FMB

Posição do racemo, do fruto e armazenamento na qualidade de sementes de mamona (Ricinus communis L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O novo padrão de desenvolvimento para o Brasil: interrelação do desenvolvimento industrial e agrícola com o meio ambiente

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