945 resultados para Azocrown ether


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Pós-graduação em Zootecnia - FCAV

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Zootecnia - FCAV

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An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of pantoprazole (CAS 102625-70-7) in human plasma using lansoprazole (CAS 103577-45-3) as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid/liquid extraction using diethyl-ether/dichloromethane (70:30; v/v) and chromatographed on a C-8 analytical column. The mobile phase consisted of acetonitrile/water/methanol (57:25:18; v/v/v) + 10 mmol/l acetic acid + 20 mmol/l ammonium acetate. The method has a chromatographic total run time of 4.5 min and was linear within the range 5.0-5,000 ng/mL. Detection was performed on a triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM). The intra- and inter-run precisions calculated from quality control (QC) samples were 4.2% and 3.2%, respectively. The accuracies as determined from QC samples were -5.0% (intra-run) and 2.0% (inter-run). The method herein described was employed in a bioequivalence study of two tablet formulations of pantoprazole.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The objective of this study was evaluate the effects of immunocastration on body weight gain, carcass characteristics and meat quality of Nellore beef cattle. Eighty Nellore beef cattle, with initial body weight of 357 +/- 8.63 kg, were placed in feedlots and distributed in two treatments (40 animals per treatments) as follow: one - non-vaccinated bulls and two - immunocastrated bulls (Bopriva (R), Pfizer Animal Health). The animals placed on treatment two were vaccinated in two doses, first application 30 days before they arrive on the feedlots and second on the day they arrive on feedlots. After 67 days of experimental period, was calculated the daily gain of live weight in kg/day of the 80 animals and selected 20 animals from each treatment for the slaughter and carcass evaluations, and ten from each group for the meat analyzes. The data were submitted to analysis of variance. Immunocastrated animals showed lower daily weight gain, hot carcass weight, carcass yield, pH, leg thickness, muscle depth, loin muscle area, carcass muscle percentage, shear force and moisture. However, this animals had higher concentration of lactate and cortisol blood, chest depth, fat thickness, finishing degree, a *, b * and c*, liquid loss in thawing process, myofibrillar fragmentation index and ether extract of meat when compared to non-vaccinated bulss. The immunocastration (Bopriva (R)) is an alternative for improving the quality of meat by the higher fat deposition and by the reduction of the shear force of the meat when compared to non-vaccinated bulls.

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The aim of this study was to evaluate the effect of different carbohydrate sources associated with sunflower oil on intake, digestibility of nutrients and nitrogen balance in diets of lambs. Twenty lambs were used, assigned in a completely randomized design with a 2 x 2 factorial arrangement of treatments with two sources of neutral detergent soluble carbohydrate, starch and neutral detergent soluble fiber, with and without the inclusion of 4.2% sunflower oil. The dry matter intake was not affected by carbohydrate sources and the inclusion of oil. Diets with a high percentage of starch provided higher digestibility of dry matter, starch and ether extract. The different sources of carbohydrates had no interference on digestibility of organic matter, crude protein, neutral detergent soluble fiber, nonfiber carbohydrates, neutral detergent fiber, hemicellulose and acid detergent fiber. The addition of oil in the diets increased the digestibility of ether extract. The combination of 4.2% oil in the diet high in soluble neutral detergent fiber had no influence on the nutrient digestibility, otherwise, the addition of oil at high starch diet caused a significant reduction in the digestibility of organic matter, neutral detergent fiber, hemicellulose and nonfiber carbohydrates. The quantities of nitrogen intake, absorbed and retained, did not differ as sources of carbohydrates and oil inclusion in the diet. The association of 4.2 % sunflower oil to the different sources of carbohydrates in the diet does not affect consumption and nitrogen metabolism in lambs. The addition of 4.2 % oil in the diet with high starch (28% of DM diet) promotes reduction in digestibility of organic matter, of fibrous and nonfibrous carbohydrate in the diet.

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The experiment was conducted to evaluate the intake, the digestibility and the performance of lambs fed with diets containing 0; 12,5 and 25% of mulberry hay in substitution of the concentrate. It was used a total of 24 lambs Ile de France breed, non castrated, average age 60 days and and 15 kg of body weight, confined, distributed in a completely randomized design. Voluntary intake of nutrients was not altered (P>0.05) by different percentages of mulberry hay inclusion in the concentrate, except ether extract which decreased linear (P<0.01). The digestibility of nutrients, days on feeding, daily weight gain and the feed conversion, were also not affected (P>0.05). The partial substitution of the concentrate, based on soybean meal and corn grain, by mulberry hay up to 25% does not compromise the intake, the digestibility of nutrients and the performance of diet of feedlot lambs.

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The uninterrupted rise in emission of greenhouse gases open way to the use of biofuels, due to politics that focus on fuel safe, clean and renewable. The use of microalgae for biodiesel production has been described as one of the most promising sources of biomass for biofuels. The aim of this study was to evaluate the extraction and lipid profile of the microalgae Dunaliella tertiolecta, Isochrysis galbana and Tetraselsim gracilis. The extractions were performed with solvents chloroform /methanol and petroleum ether. The lipid profile was analyzed by gas chromatography after transesterification.The petroleum ether showed more efficiency in the extraction, the best result obtained was in the microalgae D. tertiolecta with 19.52% of lipid. The lipid profile analysis indicated a biodiesel stable to oxidation and elevated viscosity