Allergic asthma exhaled breath metabolome: a challenge for comprehensive two-dimensional gas chromatography

Allergic asthma represents an important public health issue, most common in the paediatric population, characterized by airway inflammation that may lead to changes in volatiles secreted via the lungs. Thus, exhaled breath has potential to be a matrix with relevant metabolomic information to characterize this disease. Progress in biochemistry, health sciences and related areas depends on instrumental advances, and a high throughput and sensitive equipment such as comprehensive two-dimensional gas chromatography–time of flight mass spectrometry (GC × GC–ToFMS) was considered. GC × GC–ToFMS application in the analysis of the exhaled breath of 32 children with allergic asthma, from which 10 had also allergic rhinitis, and 27 control children allowed the identification of several hundreds of compounds belonging to different chemical families. Multivariate analysis, using Partial Least Squares-Discriminant Analysis in tandem with Monte Carlo Cross Validation was performed to assess the predictive power and to help the interpretation of recovered compounds possibly linked to oxidative stress, inflammation processes or other cellular processes that may characterize asthma. The results suggest that the model is robust, considering the high classification rate, sensitivity, and specificity. A pattern of six compounds belonging to the alkanes characterized the asthmatic population: nonane, 2,2,4,6,6-pentamethylheptane, decane, 3,6-dimethyldecane, dodecane, and tetradecane. To explore future clinical applications, and considering the future role of molecular-based methodologies, a compound set was established to rapid access of information from exhaled breath, reducing the time of data processing, and thus, becoming more expedite method for the clinical purposes.

Pré-concentração de cádmio com Saccharomyces cerevisiae e determinação em águas fluviais usando espectrometria de emissão óptica com plasma indutivamente acoplado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nota científica: padrão de distribuição de alguns bicos hidráulicos

O conhecimento sobre o padrão de deposição de bicos pulverizados é essencial para uma boa seleção e posicionamento dos mesmos em um equipamento pulverizador. O presente trabalho foi conduzido com o objetivo de se conhecer os padrões de deposição de alguns bicos pulverizadores disponíveis no mercado brasileiro. Dois ou três exemplares de cada tipo de bico foram testados em uma mesa de prova, constituída de uma chapa inclinada corrugada, construída segundo as especificações da Organização Mundial da Saúde para testes de bicos pulverizadores. Todos os bicos foram testados à pressão de 276 kN/m2 (40 libras/p ol2), com exceção dos bicos de impacto (Polijet) que foram testado s à pressão de 97 kN/m2 (14 libras/ pol 2). Ressalvando-se o pequeno número de exemplares testados por cada tipo, os resultados indicaram que: a) - existem diferenças entre a vazão estipulada pelos fabricantes e a vazão real, sendo esta diferença, acentuada para alguns casos; b) - foram observadas diferenças apreciáveis nos padrões de deposição entre as repetições nos bicos Polijet azul e Polijet vermelho (ICI) e no bico JD 14 -2 (Jacto) ; c) - os bicos 80 02 e 11003 (Spraying Systems), 80-03 e X3 (Hatsuta), JD 14-1 (Jacto) e Albuz verde (Albuz) apresentaram padrão de deposição uniforme entre as repetições; d) - considerando as variações observadas recomendam-se cuidadosos testes dos bicos, principalmente naqueles empregados nos trabalhos de experimentação, para possibilitar a escolha de um lote uniforme, para melhor controle da distribuição e dosagem dos produtos pulverizados.

Avaliação dos indicadores de desempenho de instituições federais de educação tecnológica doBrasil na abordagem do balanced scorecard

This Master of Science Thesis deals with a study on applying the BSC Balanced Scorecard to assess the performance of Federal Institution for Technological Education Institution in Brazil, a government organizations non for profit. It s accomplished a literature review in order to understand the BSC and its application to non for profit organizations and as a main result it is proposed a BSC conceptual model with an inversion of the main BSC perspective from financial to customer/society. It is used the annual management report of thirteen Institutions and applied a Pearson correlation analysis in order to verify a cause-effect situation between indicators. The main findings suggest that the model teachers qualification in terms of degree earned, quantity of teachers in full time job, and rate of students by teacher in full time job having good pearson correlation with the results expected of quality, throughput, social and demand response. However, the student unit cost use as the sole financial indicator did not get any reasonable correlation with the results, as so the quality and quantity of books in the libraries. Although it suggests a need for improvement in the model, the general model adopted appears to be satisfactory as a starting point to a BSC-like performance measuring system to this kind of Institutions

Estudo da qualidade de serviço de uma aplicação VoIP em ambientes wireless com handoff

This work deals with experimental studies about VoIP conections into WiFi 802.11b networks with handoff. Indoor and outdoor network experiments are realised to take measurements for the QoS parameters delay, throughput, jitter and packt loss. The performance parameters are obtained through the use of software tools Ekiga, Iperf and Wimanager that assure, respectvely, VoIP conection simulation, trafic network generator and metric parameters acquisition for, throughput, jitter and packt loss. The avarage delay is obtained from the measured throughput and the concept of packt virtual transmition time. The experimental data are validated based on de QoS level for each metric parameter accepted as adequated by the specialized literature

Um estudo do aumento da capacidade de sistemas 3g WCDMA usando técnicas 3.5G de expansão do enlace reverso

The introduction of new digital services in the cellular networks, in transmission rates each time more raised, has stimulated recent research that comes studying ways to increase the data communication capacity and to reduce the delays in forward and reverse links of third generation WCDMA systems. These studies have resulted in new standards, known as 3.5G, published by 3GPP group, for the evolution of the third generation of the cellular systems. In this Masters Thesis the performance of a 3G WCDMA system, with diverse base stations and thousand of users is developed with assists of the planning tool NPSW. Moreover the performance of the 3.5G techniques hybrid automatic retransmission and multi-user detection with interference cancellation, candidates for enhance the WCDMA uplink capacity, is verified by means of computational simulations in Matlab of the increase of the data communication capacity and the reduction of the delays in the retransmission of packages of information

Classificação Automática de Modulação Digital com uso de Correntropia para Ambientes de Rádio Cognitivo

Modern wireless systems employ adaptive techniques to provide high throughput while observing desired coverage, Quality of Service (QoS) and capacity. An alternative to further enhance data rate is to apply cognitive radio concepts, where a system is able to exploit unused spectrum on existing licensed bands by sensing the spectrum and opportunistically access unused portions. Techniques like Automatic Modulation Classification (AMC) could help or be vital for such scenarios. Usually, AMC implementations rely on some form of signal pre-processing, which may introduce a high computational cost or make assumptions about the received signal which may not hold (e.g. Gaussianity of noise). This work proposes a new method to perform AMC which uses a similarity measure from the Information Theoretic Learning (ITL) framework, known as correntropy coefficient. It is capable of extracting similarity measurements over a pair of random processes using higher order statistics, yielding in better similarity estimations than by using e.g. correlation coefficient. Experiments carried out by means of computer simulation show that the technique proposed in this paper presents a high rate success in classification of digital modulation, even in the presence of additive white gaussian noise (AWGN)

Estudo da hidrodinâmica de um novo separador líquido-líquido: aplicação ao sistema óleo-bruto/água produzida

This work aims to use a different hydrodynamic condition applied to a new design of mixer-settler on treating wastewater produced by petroleum industry, called MDIF (Misturador-Decantador à Inversão de Fases/ Mixer-Settler based on Phase Inversion MSPI). The use of this different hydrodynamic behaviour is possible due to vertical disposition of the device and the principle of Phase Inversion that controls the MDIF, providing the generation (creation) of a cascade of drops, into an organic layer, that works as micro-decanters, thus making possible the formation of a bed of non-coalesced drops, called Bed Formation . The use of this new hydrodynamics condition allows to increase the residence time of the oil carrier drops, into an organic layer, and the device can treat a greater volume of wastewater. In view of to get this condition it is necessary to operate at high throughput (58,6 m3.m-2.h-1). By results, the condition of Bed Formation is the best one to be used when MSPI operates with throughput up to 58,6 m3.m-2.h-1. The results using the condition of Bed Formation show that increasing the height of the bed of non-coalesced drops and/or decreasing the volumetric ratio (O/A) an increase of the separation efficiency is detected

Interspecific fish hybrids in Brazil: management of genetic resources for sustainable use

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Isolation and molecular identification of wine yeasts from a Brazilian vineyard

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Synchrotron X-ray powder diffraction data of atorvastatin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of Lubricating Oil Preparation Procedures for the Determination of Al, Ba, Mo, Si and V by High-Resolution Continuum Source FAAS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Direct determination of calcium in milk by atomic absorption spectrometry using flow-injection analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Arquitetura multiprocessada e reconfigurável para a síntese de redes de Petri em hardware

Uma arquitetura reconfigurável e multiprocessada para a implementação física de Redes de Petri foi desenvolvida em VHDL e mapeada sobre um FPGA. Convencionalmente, as Redes de Petri são transformadas em uma linguagem de descrição de hardware no nível de transferências entre registradores e um processo de síntese de alto nível é utilizado para gerar as funções booleanas e tabelas de transição de estado para que se possa, finalmente, mapeá-las num FPGA (Morris et al., 2000) (Soto and Pereira, 2001). A arquitetura proposta possui blocos lógicos reconfiguráveis desenvolvidos exclusivamente para a implementação dos lugares e das transições da rede, não sendo necessária a descrição da rede em níveis de abstração intermediários e nem a utilização de um processo de síntese para realizar o mapeamento da rede na arquitetura. A arquitetura permite o mapeamento de modelos de Redes de Petri com diferenciação entre as marcas e associação de tempo no disparo das transições, sendo composta por um arranjo de processadores reconfiguráveis, cada um dos quais representando o comportamento de uma transição da Rede de Petri a ser mapeada e por um sistema de comunicação, implementado por um conjunto de roteadores que são capazes de enviar pacotes de dados de um processador reconfigurável a outro. A arquitetura proposta foi validada num FPGA de 10.570 elementos lógicos com uma topologia que permitiu a implementação de Redes de Petri de até 9 transições e 36 lugares, atingindo uma latência de 15,4ns e uma vazão de até 17,12GB/s com uma freqüência de operação de 64,58MHz.

Estimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers

Eucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side of the cross, knowledge of outcrossing versus selling rates is essential for maintaining adequate levels of genetic variability for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24 individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability within families. The multilcous outcrossing rate (t(m)) estimated from RAPD markers (0.93 +/- 0.027), although in the same range, was higher (alpha > 0.01) than the estimate based on AFLP (0.89 +/- 0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated Wright's fixation index was lower than expected based on t, possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of t,. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding and natural populations of forest trees. We anticipate an increasing use of such PCR-based technologies in mating-system studies, in view of their high throughput and universality of the reagents, particularly for species where isozyme systems have not yet been optimized.