932 resultados para stepwise selection to cefotaxime resistance


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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The administration of viable Bifidobacterium animalis was tested to induce resistance against Strongyloides venezuelensis infection in mice. Effects on parasite burden, worm length, egg output, and intestinal mucosal histology were evaluated. The oral administration of B. animalis, strain 04450B, starting 14 days before the inoculation of nematode larvae significantly decreased the worm burden and egg output. In probiotic treated animals, the percent reduction of adult worms in the intestine was of 33% and the reduction of egg production was of 21%, compared with those of the control group. The duodenum villous height and villous/crypt ratio were significantly higher in probiotic-treated mice, indicating that this group could be experiencing less intestinal damage. The present findings revealed that the administration of B. animalis for the amelioration of host response to nematode infections is biologically plausible and could have some potential for impacting public health. Meanwhile, further study is needed to delineate the nature and identity of the factor(s) involved in these beneficial effects.

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Pós-graduação em Fisiopatologia em Clínica Médica - FMB

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Proteção de Plantas) - FCA

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Medicina Veterinária - FCAV

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Patients that are mechanically ventilated in ICUs are constantly exposed to different pathogens, which present multiantibiotic resistance. Among these microorganisms, is MRSA (Meticillin-Resistant Staphylococcus aureus) considered to be a therapeutic challenge due to its resistance to β-lactam antibiotics. Therefore, this study proposed to identify species of Staphylococcus spp. isolated from mechanically ventilated patients in ICU, the gene mecA detection and the genes of the enterotoxins A (sea), B (seb), C (sec-1) and D (sed) in samples of S. aureus, as well as the phenotypic resistance determination to oxacillin using the disc-diffusion method with discs of oxacillin and cefoxitin. The samples collection occurred during in a period of 19 months, obtaining samples from 232 patients. A percentage of 39% (70) of Gram-positive cocci were found; which 82,8% (58) were identified as Staphylococcus spp,. among these, 75,8% (44) corresponded to S. aureus species and 47,7% were identified as MRSA. It was found resistance to both drugs in 31,8% of the S. aureus samples, 16 (36,3%) had the gene sea and 11 (25%) had the sec-1 gene. Among the coagulase-negative staphylococci obtained, the species most found was S. epidermidis, corresponding to 43% (6). The results revealed that one of the most important etiologic agents of VAP amid the Gram-positive cocci is the species S. aureus, with special attention to MRSA. The presence of enterotoxins genes in S. aureus did not showed determinant role in VAP, but the presence of these superantigens can contribute worsening the patient’s prognosis, since they are associated with intense inflammatory response

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Pós-graduação em Engenharia Mecânica - FEG

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Genética - IBILCE