Avaliação da densidade mineral óssea em mulheres na pós-menopausa tratadas de câncer e mama

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Avaliação da sensibilidade insulínica, transdução do sinal insulínico e da densidade mineral óssea em ratas tratadas com fluoreto de sódio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise histomorfométrica do reparo ósseo em tíbias de ratos castrados submetidos ao efeito do alendronato sódico

This study used histomorphometric analysis to investigate the effect of sodium alendronate, used for the treatment of osteoporosis, on the repair of surgically-induced bone defects in the tibia of castrated rats. Methods: The castrated animals were given subcutaneous injections of sodium alendronate (0.7mg/Kg) diluted in saline once a week; the control animals were given the same dose of saline. At 16, 30 and 44 days after the first injection of sodium alendronate, the animals were sacrificed and the right tibias were removed and processed for histomorphometric analysis. The volumetric bone mineral density was estimated by a reticular grid (25 points) attached to a light microscope. The number of points on the bone tissue was counted in the histological sections, totaling 100 points/animal. Results: The results revealed that sodium alendronate stimulated bone formation in castrated rats in all occasions, mainly at 16 and 30 days. Conclusion: Sodium alendronate affects mineral homeostasis, promoting bone repair.

Análise histomorfométrica da influência do Alendronato sódico no processo de reparo ósseo em tíbia de ratas submetidas a ovariectomia

The sodium alendronate (AS), considered as inhibitor in the osteoclasts- mediated bone resorption, promotes final effect of inhibition of resorption and increases bone mass. The objective of this research was to assess, by histomorphometry, the effect of sodium alendronate in repair bone of ovariectomized rats, in which there was performed a bone defect in the right tibia. The treated rats received a subcutaneous injection of sodic alendronate once a week, at 0.7 mg / kg, diluted with saline solution; the controls received the same volume of saline solution. In the periods of 16, 30 and 44 days after the first dose of AS, the animals were sacrificed, the right tibia was removed and processed for histomorphometric analysis. Four non-serial fields were used for the density volume quantification utilizing an integrative eyepiece with 25 points, totalizing 100 points per animal. Based on the results, the present study concludes that the ovariectomy induced osteoporosis and that the AS stimulated the bone formation. In addition, the ovariectomy decreased the estrogen levels. However, this procedure did not significantly influence the action of sodic alendronate.

Avaliação do reparo ósseo na interface osso/implante em ratas com osteoporose induzida tratadas com raloxifeno ou alendronato: análise histométrica, imunoistoquímica, por epifluorescência e biomecânica

Pós-graduação em Odontologia - FOA

Efeito da ovariectomia e da reposição hormonal no reimplante dentário imediato: análise histomorfométrica e imunoistoquímica em ratas

Pós-graduação em Odontologia - FOA

Programa computacional ODR-ATA para densitometria óssea baseado na densitometria radiografica

Pós-graduação em Ciência Animal - FMVA

Low-dose combined oral contraceptive use is associated with lower bone mineral content variation in adolescents over a 1-year period

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alveolar bone dynamics in osteoporotic rats treated with raloxifene or alendronate: confocal microscopy analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Raloxifene enhances peri-implant bone healing in osteoporotic rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of alendronate sodium on tooth movement in ovariectomized rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

In order to standardize an experimental model to study the effects of absence of ovarian hormones in maxillary bones compared with long bones, the aim of this research was to analyze the influence of ovariectomy (OVX) on rat alveolar bone and tibiae, in different observation periods. Thirty-six female rats were ovariectomized or sham operated. After 60, 90 or 120 days, the animals were sacrificed and their hemimandibles, maxillae and tibiae were removed and routinely prepared for hematoxylin and eosin staining. The percentage of bone matrix area in bone septum in the first molar furcation region, and in tibial metaphysis was calculated, and data were submitted to statistical analysis (p < 0.05). As regards the histomorphometrical analysis in jaw bones, there was no statistical difference between groups, while the effects of ovariectomy on tibiae were seen as early as 60 days. According to the methods used, there was no significant influence of absence of ovarian hormones on interradicular septum of mandibular or maxillary first molars in the periods studied, despite the reduction in bone matrix area in tibia metaphysis as early as 60 days. (C) 2014 Elsevier GmbH. All rights reserved.

Factors associated with sarcopenia in subjects aged 80 years and over

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bone mineral density evaluation in rats submitted to daily diet of coffee and soft drinks

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency

The aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21,45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers.