Depth ranges of calcareous nannofossil datums from ODP Holes 113-689B and 113-690B

Magnetostratigraphic studies of Paleogene sediments piston-cored on Maud Rise, Weddell Sea (ODP Sites 689 and 690), are a cornerstone of Southern Ocean Paleogene and Neogene chronostratigraphy. However, parts of previous magnetostratigraphic interpretations have been called into question, and recent reinvestigation of the upper Paleocene-middle Eocene portion of Site 690 suggested that the records might be contaminated by spurious magnetizations, which raises doubts about the reliability of these important records. We undertook a high-resolution magnetostratigraphic study of Eocene-Oligocene u-channel samples from ODP Holes 689B, 689D, 690B, and 690C in order to address these concerns. A pervasive overprint appears to be present below the middle Eocene, which compromises magnetobiostratigraphic interpretations for the upper Cretaceous and lower Paleogene. Nevertheless, our new results provide a robust record of geomagnetic field behavior from 38.5 to 25 Ma and confirm the reliability of these sediments for calibration of biostratigraphic datum events during a crucial phase of earth history when major Antarctic ice sheets developed. Also, comparison of magnetozone thicknesses in multiple holes at the same site indicates that ~1.2-1.8 m of the stratigraphic record is missing at each core break, which corresponds to time breaks of 120-360 k.y. Lack of a continuous record within a single hole renders useless spectral analyses for investigating long geomagnetic and paleoclimatic time series. This observation reinforces the need for coring of multiple offset holes to obtain continuous paleoceanographic records. Sedimentary hiatuses have been identified only at the deeper of the two investigated sites (Site 690), which could mark a local response to the onset of the Antarctic Circumpolar Current.

Temperature Dependent Effects of Elevated CO2 on Shell Composition and Mechanical Properties of Hydroides elegans: Insights from a Multiple Stressor Experiment

The majority of marine benthic invertebrates protect themselves from predators by producing calcareous tubes or shells that have remarkable mechanical strength. An elevation of CO2 or a decrease in pH in the environment can reduce intracellular pH at the site of calcification and thus interfere with animal's ability to accrete CaCO3. In nature, decreased pH in combination with stressors associated with climate change may result in the animal producing severely damaged and mechanically weak tubes. This study investigated how the interaction of environmental drivers affects production of calcareous tubes by the serpulid tubeworm, Hydroides elegans. In a factorial manipulative experiment, we analyzed the effects of pH (8.1 and 7.8), salinity (34 and 27), and temperature (23°C and 29°C) on the biomineral composition, ultrastructure and mechanical properties of the tubes. At an elevated temperature of 29°C, the tube calcite/aragonite ratio and Mg/Ca ratio were both increased, the Sr/Ca ratio was decreased, and the amorphous CaCO3 content was reduced. Notably, at elevated temperature with decreased pH and reduced salinity, the constructed tubes had a more compact ultrastructure with enhanced hardness and elasticity compared to decreased pH at ambient temperature. Thus, elevated temperature rescued the decreased pH-induced tube impairments. This indicates that tubeworms are likely to thrive in early subtropical summer climate. In the context of climate change, tubeworms could be resilient to the projected near-future decreased pH or salinity as long as surface seawater temperature rise at least by 4°C.

Turbidity measurements in 96-wells microplates to determine the densities of copiotrophic and oligotrophic bacteria with the Most-Probable-Number-method during POLARSTERN cruise ANT-XI/2

A new microtiter-plate dilution method was applied during the expedition ANTARKTIS-XI/2 with RV Polarstern to determine the distribution of copiotrophic and oligotrophic bacteria in the water columns at polar fronts. Twofold serial dilutions were performed with an eight-channel Electrapette in 96-wells plates by mixing 150 µl of seawater with 150 µl of copiotrophic or olitrophic Trypticase-Broth, three times per well. After incubation of about 6 month at 2 °C, turbidities were measured with an eight-channel photometer at 405 nm and combinations of positive test results for three consecutive dilutions chosen and compared with a Most Probable Number table, calculated for 8 replicates and twofold serial dilutions. Densities of 12 to 661 cells/ml for copiotrophs, and 1 to 39 cells/ml for oligotrophs were found. Colony Forming Units on copiotrophic Trypticase-Agar were between 6 and 847 cells/ml, which is in the same range as determined with the MPN method.