Polysiloxane-poly(propylene oxide) hybrid discs as solid phase in anti-HCV detection using a recombinant core protein

In this work, siloxane-poly(propylene oxide) discs (PPO disc) prepared using the sol-gel process were used as solid phase in enzyme-linked immunosorbent assays (ELISA) for the detection of anti-hepatitis C virus (HCV) antibodies. The HCV RNA from serum (genotype 1b) was submitted to the RT-PCR technique and subsequent amplification of the HCV core 408 pb. This fragment was cloned into expression vector pET42a and expressed in Escherichia coli as recombinant protein with glutathione S-transferase (GST). Cell cultures were grown and induced having a final concentration of 0.4 x 10(-3) mol L-1 of IPTG. After induction, the cells were harvested and the soluble fraction was analyzed using polyacrilamide gel 15% showing a band with an approximate molecular weight of 44 kDa, the expected size for this GST-fused recombinant protein. The recombinant protein was purified and continued by immunological detection using HCV-positive serum and showed no cross-reactivity with positive samples for other infectious diseases. An ELISA was established using 1.25 ng of recombinant protein per PPO disc, a dilution of 1: 10,000 and 1:40 for a peroxidase conjugate and serum, respectively, and solutions of hydrogen peroxide and 3,3',5,5'-tetra-methylbenzidine in a ratio of 1: 1. The proposed methodology was compared with the ELISA conventional polystyrene-plate procedure and the performance of the PPO discs as a matrix for immunodetection gave an easy synthesis, good performance and reproducibility for commercial application. (c) 2007 Elsevier B.V. All rights reserved.

Drug-matrix interaction of sodium diclofenac incorporated into ureasilpoly(ethylene oxide) hybrid materials

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Corrosion protection of stainless steel by polysiloxane hybrid coatings prepared using the sol-gel process

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cryopreservation of Dendrobium hybrid seeds and protocorms as affected by phloroglucinol and Supercool X1000

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

On the Milnor fibre and L numbers of semi-weighted homogeneous arrangements

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Single-phase high power factor hybrid rectifier suitable for high-power applications

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of Synthesis Conditions on the Nanostructure of Hybrid Sols Produced by the Hydrolytic Condensation of (3-Methacryloxypropyl)trimethoxysilane

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ligand Exchange Inducing Efficient Incorporation of CisPt Derivatives into Ureasil-PPO Hybrid and Their Interactions with the Multifunctional Hybrid Network

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Controlled Cisplatin Delivery from Ureasil-PEO1900 Hybrid Matrix

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B-bubalis) chromosome 20

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chromosome numbers and their taxonomic implications for eight Brazilian andropogons (Poaceae: Andropogoneae)

Eleven chromosome counts are reported for Brazilian populations of eight species of Andropogon. Counts for five of the species are first reports for the species. Four of these species, A. arenarius, A. glaziovii, A. lateralis, and A. lindmanii, are hexaploids (n=30) and belong to a group of about a dozen species informally referred to as the Andropogon lateralis complex. Two other species of this complex, A. bicornis and A. hypogynus, we also found to be hexaploids, in agreement with previous reports. Diploid counts (n=10) for A. macrothrix (a first report) and A. virgatus (the same as two previous reports) support the morphological affinities of these two species to other species complexes within Andropogon. © 1986 the New York Botanical Garden.

Molecular evidence of ribosomal DNA (rDNA) amplification of a minichromosome derived from Drosophila arizonae in D. mulleri-D. arizonae hybrid males

We present data supporting cytogenetic observations on nucleolar dominance in hybrids between Drosophila arizonae and D. mulleri. Our approach was to compare the rDNA restriction patterns between the parental species and their hybrids. Results demonstrated that the minichromosome attached to the nucleolus in hybrid males is derived from D. arizonae.

Differences in the number of sensilla coeloconica and sensilla ampullacea of segment 10 of the antennae of Africanized and Caucasian bees and of their F1 hybrids

The numbers of sensilla coeloconica and sensilla ampullacea of segment 10 of the antennae of Caucasian and Africanized worker bees and of their hybrids were counted with the aid of a scanning electron microscope. Africanized bees have fewer sensilla than Caucasian bees and the continuous distribution obtained from the F1 values suggests polygenic inheritance for the control of this trait. There are also indications that the hybrid Brazilian Apis mellifera studied here are close to typical African bees (Apis mellifera scutellata) in terms of number of sensilla.

Chromosome numbers of some Brazilian species of diplopods (Diplopoda, Arthropoda)

The present paper presents the chromosome numbers of five brazilian species of diplopods: Plusioporus setiger (2n = 10 and 2n= 10+ lB, the distinction of the sexual pair was not possible), Pseudonannolene ophiulus (2n=12, XY), Pseudonannolene halophila (2n=16, XY), Rhinocricus sp. (2n=28, XY) and Rhinocricus padbergi (2n=20, the distinction of the sexual pair was not possible).

Parasitism of Greenbugs (Homoptera: Aphididae) by Lysiphlebus testaceipes (Hymenoptera: Braconidae) in Grain Sorghum: Implications for Augmentative Biological Control

Field cage studies were conducted to describe the relationship between the percentage of Lysiphlebus testaceipes (Cresson) parasitism (as measured by aphid mummies) and densities of greenbug, Schizaphis graminum Rondani, on grain sorghum, Sorghum bicolor L. In 1993 and 1994, a biotype E-susceptible grain sorghum hybrid was grown in field cages and L. testaceipes adults were released after each plant was infested with 20 biotype E greenbugs. The release rates were 0, 0.5, 1.0, and 2.0 wasps per plant in 1993, and 0, 0.16, 0.33, and 0.5 wasps per plant in 1994. Greenbugs and mummies were counted 1-2 times a week on all leaves of 2-4 randomly selected plants per cage. A release rate of 0.33-0.5 wasps per plant infested with 20 greenbugs maximized the number of mummies produced and prevented the greenbugs from reaching an economic threshold of 1,000 greenbugs per plant. Peak numbers of mummies occurred ≈400-500 DD (10°C base) after the initial wasp release. Regression analyses showed that the greenbug population started decreasing when the percentage of parasitism (as measured by mummies) reached 20-30 %. Greenbugs in the absence of wasps significantly reduced yield in 1994, but not in 1993.