Desenvolvimento e validação de uma técnica quantitativa de análise de imagem para avaliação do teste do cometa corado pela prata

The comet assay is a method of DNA damage analysis widely used to quantify oxidative damage, crosslinks of DNA, apoptosis and genotoxicity of chemicals substances as chemical, pharmaceuticals, agrochemicals products, among others. This technique is suitable to detect DNA strand breaks, alkali-labile sites and incomplete excision repair sites and is based on the migration of DNA fragments by microeletroforesis, DNA migrates for the anode forming a “tail”, and the formed image has the appearance of a comet. The slides can be stained with fluorescence or silver, having differences in the microscopy type used for the analysis and the possibility of storage of the slides, moreover, the first one is a stained-method with more difficulties of accomplishment. The image analysis can be performed by a visual way, however, there is a disadvantage as the subjectivity on the results, that can be minimized by an automated method of digital analysis. This process was studied in this report with the aim to perceive the validation of the digital analysis turning it a quantitative method with larger reproductibility, minimizing the variability and imprecision due to the subjective analysis. For this validation we selected 50 comets photographed in a standardized way and printed, afterwards, pictures were submitted to three experienced appraisers, who quantified them manually. Later, the images were processed by free software ImageJ 1.38x, printed and quantified manually by the same appraisers. The intraclass correlation was higher to comet measures after image processing. Following, an algorithm of automated digital analysis from the measures of the comet was developed; the values obtained were compared with those 12 estimated manually after the processing resulting high correlation among the measures. The use of image analysis systems increases ...(Complete abstract click electronic access below)

Um mundo radicalmente melhor: criatividade, colaboração e padrão de redes como estratégias para o desenvolvimento sustentável

There are substantial evidences that the period experienced by humanity globally is unprecedented and is heading towards a major transformation that results from the Globalization. Totally conditioned to the addictions of the dominant and predatory capitalism, humanity has, for decades, exhausted natural resources, disregarded the nature of its own social existence and walked away from its humanity. It is notable, however, an impressive flow of factors that dialogue and support each other as trends that go towards sustainable development, based on the harmonious integration between Technology, Culture, Society, Environment and Economy. This emerging moment can be seen from the perspectives of the Creative Economy as economic paradigm centered on the subjectivity and the human capacity to undertake innovative services, products and solutions guided by social values. Within this fluid and dynamic global context, initiatives that legitimately intend to act sustainably are gaining space. This socioeconomic moment fosters and is fostered by new kinds of work and organization guided by the Collaboration and social structuring on Network Patterns. These new social models significantly transform the understanding and insights about the Communication flows. The HUB São Paulo, as creative and social organization that operates under the logic of Collaboration through a Network Structure, was the subject of a case study used to sustain the defense of this emerging scenario and also to perform an analysis on the new role of Communication, at perspectives of transformation of mental paradigms towards sustainability and establishment of meaningful connections

História de vida e formação: educando o olhar e percebendo pesquisa enquanto experiência

Way in the theme of the story of my life and training and electingchildhood as a guide in the works of Benjamin. Narrating a series of reflections on childhood and experience, taking for reading and interpretation of aphorisms that make up my childhood and training as a fellow researcher, a full text simultaneously, is juxtaposed with other texts and authors, intertwining with Benjamin, memory and history in my narrative, in a singular moment, an event. In this course highlight the place ofexperience and their languages, between the know-how, andknowing how to express thinking about childhood and educationevent in the making, which announces the issue of impoverishment and destitution of the experience of life ineducational practice, indicating the possibility resume thembetween school knowledge and practices through different view of childhood and the event, as a teacher. I will continue outliningmy experience in research groups choosing the language and itsinterfaces specifically with art cinema / pictures in the construction of our subjectivity in postmodernity. The methodology is qualitative, will take place within what we call the ethical self. Transcendence is an act and not a process, and yetas an overshooting of history is always historically situated and,therefore, has a concrete context. Using my own journey as an educator / researcher in my path as an area of passage / travel / experience / track / time in the constitution of my subjectivity in my own life story

Relações Públicas: a importância das relações humanas

This monograph has as objective to study the human relations and the Public Relations in the contemporary time in witch the new technologies of the information got closer to the various existent cultures, but who make of the informative society of the global era, the expansion of the capital and of the culture of the more value. Man kind is backing away from the human relations, because the social interactions in the global world are done by objects such as the television, the internet and the telephone in witch cases the subjectivity and the human contact are traded for the informative quantity, because man kind doesn’t have anymore time to think, reflect and to exchange information. Man kind isolates itself and fills it’s subjective emptiness with the products offered by the neoliberal capitalism. The public relations in the organizations seek the human values created from a complex network of social interactions in all the heterogeneous of the organization. Through the communication the public relations tries to manage the relationships with the dialogical communication, between the public-organization, to harmonize the conflicting differences of the organizational environment. Thus, the public relations has to assume the roll of the information manager, and administrator of the human relations in this new global world, informative and that functions from the capital

Dinâmicas de segregação sócio-espacial no meio urbano

In this study we discuss a range of topics related to urban development from the perspective of connection points between the existence of the individual subjects’ lives. In this connection we identified the signs that express an articulation among subjectivity processes and conditions for interference in the real world, relying on circumscribed remarks about spatial configurations and its uses. This way, the substance of our research is structured upon the concept of fashion brands operating new dynamics of urban lifestyles, a strengthened reliance on the physical body’s appearance and the profusion of a supposed “I”, presented as a spectacle. Taking these assertions as new “trends” on building view-points and contemporary cities, we discuss the implications of these currents on how people gather and organize themselves in the urban landscape, making an effort to comprehend how these vogues reverberate on the (re)production of increasingly segregated cities

Implicações do ensino de arte nos anos iniciais: o trabalho com a leitura de imagens

This paper aims to address initially the implications and consequences of the planned use of image reading in classrooms of Art in the early years of elementary school. Enumerating some contributions to enrich and develop narrative skills, descriptive, analytical and interpretative. Thus, in a next goal will be researched the importance and essentiality of working with the reading of images, not only in art classes but at other opportunities, working intertextuality. Mobilizing both rationality and imagination, enhancing the information and content as well as encouraging creativity and analytical formation of subjectivity. Aiming at meaningful learning and encouraging other views and ways of thinking, feeling, teach, learn and be

A pluralidade narrativa do cinema e a crítica negligente: estudo de caso : anticristo e melancolia, de Lars Von Trier

The cinematographic language is notedly plural and heterogeneous. There are many filmic elements that “narrate” all the time, in different ways. Besides enumerating some of them, this study seeks to analyse if the criticism is attentive to all of these tools or if it sticks only to some of them. It’s also reflects if this activity uses more objectives or subjectives aspects as basis for its conclusions. Here, a very specific field was chosen: the study is done by a comparative analysis of the reception of Brazilian’s and European’s criticism from two Lars Von Trier’s films, Antichrist and Melancholia. After explaining how the narrative elements are used in the cinema, it’s demonstrated that, in general, the criticism is negligent and does not analyze all of those tools. In some cases, the analysis of some elements is done in a superficial way, so others aspects can be highlighted

A prática de treinamento dos corpos dentro da empresa: um estudo sobre uma ferramenta de controle da qualidade

Befor the vast economical growth and wild scientific and technological development, the companies are demanding, more and more qualification and acting of the services rendered by their employees. Like this, tasks are restricted the they be done with excellence, because the companies are always controlling the worker's development. They are several the tools used by the companies, as acting indexes to control the course of the worker's activities or certain section, integrations for new employees with the objective to present the line of work of the company so that the new employee is framed in these patterns, trainings offered for the employees to carry out their services with quality and compromising, evaluations of year end in order to test the worker's knowledge and to demand better results, finally, the companies hope the employee integrates normativamente into the reality of the company, in way to contribute for the atingimento of the organizational strategies, always collecting for this to obtain the best results and contribute to the growth of the company. This way, that research will present a tool of control of the total quality applied in a company in Rio Clearing's city and that it is managed by an educador. They will be verified the objectives that the company has in relationship a this control, the worker's subjectivity as participant subject of the tool of control of the System of Administration of the Quality and the worker's different glances before these aspects throught an interview.

Análise quantitativa dos parâmetros físicos e radiométricos de procedimetnos radioterapicos em tumores de mama

The paper presents the radiometric parameters determined by the medical physicist during routine radiotherapy planning service in cases of breast cancer . The contours of the breast volume in patients undergoing radiation breast tumors at the Hospital das Clinicas, Faculty of Medicine , UNESP, Botucatu ( HCFMB ) during the year 2012 were analyzed . In order to analyze the influence of physical and radiometric parameters for the determination of the dose distribution of irradiated breast volume , four measurements of isodose curves were prepared in four different heights breast , and compared with the isodose curves plotted computationally . In the routine of planning , the medical physicist must determine the isodose curve that gives the best dose distribution homogeneity in the irradiated volume . The choice of the treatment plan can be done by dedicated computer systems , which require significantly costly investments available services having better financial support . In the Service of Medical Physics , Department of Radiotherapy , HC FMB , we use a two-dimensional software for determination of isodose curves , however , this software is out of date and frequently becomes inoperable due to the lack of maintenance and it is a closed system without feasibility of interference from computer professionals . This fact requires manual preparation of isodose curves , which are subject to uncertainties due to the subjectivity in the clinical interpretation of medical radiation oncologist and medical physicist responsible for planning , plus dispendiar significant calculation time . The choice of the optimal isodose curve depends on the energy of the radiation beam , the geometry and dimensions of the irradiated area . The contours of the breast studied in this work evaluations showed that , for a given energy input , such as the energy of 1.25 MeV of gamma radiation Unit Telecobaltoterapia , the determination of the percentage depth dose ( PDP ) ...

Modelagem para identificação de indicadores de eficiência energética para edificações e plantas industriais

Pós-graduação em Engenharia Mecânica - FEG

Risco, saúde e obesidade na prática de trabalho dos policiais militares do Estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Artefoto : a ênfase fotográfica

Pós-graduação em Artes - IA

O ethos das obras de autoajuda para terceira idade

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O relógio do Rosário anuncia A máquina do mundo: Haroldo de Campos relê Drummond

Considering fragments of the haroldian poem A Máquina do Mundo Repensada, this paper approximates Drummond and Haroldo de Campos. In this poem, the poetic person’s unquietness conducts him to very peculiar displacements: the one that concerns to the end of millennium, marked by the utopia’s collapse, by the subjectivity fragmentation, by the frontiers’ reconfiguration between people and places. Assuming what the drummondian fatigued eyes have seen, the poet Haroldo de Campos will (re)think his world disharmony

Literatura, cinema e outras arquiteturas textuais: algumas considerações sobre teorias da adaptação

Studies investigating the relationship between literature and film have been largely oriented by an analysis vector which always departs from literary texts towards films. Moreover, the overwhelming majority of criticism done by renowned theorists such as Robert Stam and Brian McFarlane approaches almost exclusively texts considered canonical. This reveals an overemphasis on the notion that the “primordial” text in a study of adaptation should be the literary text. This essay discusses some of those concepts, challenging the “binary” models in adaptation studies and showing how the vectors of analysis can be usefully reversed, for example, starting from films to literature and to other textual architectures. This approach, shared by theorists such as Linda Hutcheon (2006) and Thomas Leitch (2007), rejects old notions that guided comparisons between literary and filmic texts, such as fidelity and equivalence, replacing them with intertextuality and transmedia storytelling.