Processo de modificação genética de bactéria acidófilas e construção de um vetor de transformação

The present invention describes a method for transforming chemolithotrophic acidophilic bacteria using electroporation technology. The proposed method allows transforming a bacterial line using a transformation vector, the pAF vector, which contains an origin of vegetative replication that allows the vector to replicate inside the bacteria without altering the natural physiological functions of the latter. Also disclosed is the use of the bacteria modified according to the invention in bioleaching processes of sulphated copper, gold, uranium, nickel, zinc and cobalt ore, inter alia.

Accumulation of dry mass and macronutrients by sourgrass plants

The experiment was carried out aiming to analyze the dry mass production and distribution and the content and accumulation of macronutrients in sourgrass (Digitaria insularis) plants cultivated under mineral nutrition standard conditions. Plants grew in 7-liter pots filled with sand substrate and daily irrigated with nutrient solution, being maintained under greenhouse conditions. Treatments consisted of times of evaluation (21, 35, 49, 63, 77, 91, 105, 119, and 133 days after emergence - DAE) and were arranged in a completely randomized design with four replicates. Sourgrass showed small accumulation of dry mass (0.3 g per plant) and macronutrients (3.7 mg of N per plant, 0.4 mg of P per plant, 5.6 mg of K per plant, 0.9 mg of Ca per plant, 0.7 mg of Mg per plant, and 0.3 mg of S per plant) at vegetative growth stage (< 49 DAE). Those accumulations increased mainly after 77 DAE, reaching the maximum theoretical value at 143, 135, 141, 129, 125, 120, and 128 DAE, for dry mass (12.4 g per plant), N (163.2 mg per plant), P (27.1 mg per plant), K (260.5 mg per plant), Ca (47.6 mg per plant), Mg (30.9 mg per plant), and S (13.7 mg per plant), respectively. K and N were found with higher rates and, as a consequence, they were required and accumulated in greater amounts in plant tissues of sourgrass.

Crescimento e nutrição mineral de Sida rhombifolia

This experiment was carried out to analyze dry mass production and distribution, and the content and accumulation of macronutrients in arrowleaf sida (Sida rhombifolia) plants cultivated under mineral nutrition standard conditions. Plants grew in seven liter pots filled with sand substrate and daily irrigated with nutrient solution, under greenhouse conditions. Treatments consisted of times of evaluation (21, 35, 49, 63, 77, 91, 105, 119, and 133 days after emergence - DAE) and were arranged in a completely randomized design with four replicates. Arrowleaf sida plants showed small accumulation of dry mass (0.3 g per plant) and macronutrients (6.9 mg N per plant, 0.7 mg P per plant, 8.6 mg K per plant, 4.9 mg Ca per plant, 2,6 mg Mg per plant, and 0.3 mg S per plant) at the vegetative growth stage (< 49 DAE). Those accumulations increased, mainly after 63 DAE, and the daily accumulation rate was crescent up to 94 DAE (dry mass - DM), 89 DAE (N and P), 98 DAE (K), 95 DAE (Ca and S), and 93 DAE (Mg), when there was accumulation of 26.3 g DM per plant, 402.6 mg N per plant, 45.6 mg P per plant, 359.3 mg K per plant, 337.6 mg Ca per plant, 71.9 mg Mg per plant, and 20.9 mg S per plant. N and K had the highest rates and, consequently, were the most required and accumulated in greater amounts in plant tissues of arrowleaf sida.

Identificação da dinâmica espaço-temporal para estimar área cultivada de soja a partir de imagens MODIS no Rio Grande do Sul

The objective of this study was to define a method for estimating soybean crop area in the Northern Rio Grande do Sul state (Brazil). Overall, six different remote sensing methods were proposed based on spectral-temporal profile and minimum and maximum values of NDVI/MODIS related to the stages of sowing, maximum development and harvesting of soybean areas. The resulting estimates were compared to official crop area data provided by the Brazilian government, using statistical analysis and the fuzzy similarity method. The performance of each method depended on information such as crop size, type of crop management, and sowing/harvesting dates. Regression coefficients of determination and fuzzy agreement values were above 0.8 and 0.45, respectively, for all methods. For operational monitoring of soybean crop area, the empirical threshold applied to the image difference with inclusion of harvest image method was the most effective, producing estimates that matched closely the official data. For spatial analysis the application of multitemporal images classification method is recommended that generated a map of better quality. The efficiency of these methods should be evaluated in the areas of soybean expansion in the state.

Estaquia em Anonas

Annonaceae is an important family of plants that constitutes the Brazilian flora. Anona species produce fruits for fresh consumption, medicinal use and other purposes. The aim of this study is to present information of literature about vegetative propagation by cutting of some Annonaceae species with potential for consumption and as rootstock. As conclusion, the literature presents a range of rooting results, even about the same species. The rooting success depends on the species, variety, stock plant, season, plant growth regulators, rooting environment and juvenility. Some species present rooting potential, but there are lack of literature about plant management and orchard installation. The use of plant growth regulators improves the rooting of the most species with potential to propagate by cuttings.

Efeito do porta-enxerto e da época de poda na duração das fases fenológicas e no acúmulo de graus-dia pela videira 'Niagara Rosada'

It was evaluated the influence of rootstocks 'IAC 766', 'IAC 572', 'IAC 313', 'IAC 571-6' and 'Ripária do Traviú' and the pruning periods in the duration of phenological stages and in the accumulation of degree-days on grape 'Niagara Rosada' .The experiment was conducted in Louveira, SP. The treatments consisted of five rootstocks and three times of pruning, the experimental design being used in a completely randomized split plot with five replications, and plots represented by rootstocks and the subplots were pruning. After pruning the grapevine, were realized evaluations for phenological stages, using the criterion of Eichhon e Lorenz (1984). ). In three seasons of pruning, based on phenological stages, it was calculated the length of the periods: pruning to budding, pruning to the beginning of the flowering, pruning to the beginning of fruiting, pruning to beginning of ripening of the bunches, pruning the beginning of the harvest. Taking as a basis the cycle length of the vine and the average daytime temperatures, it was calculated the accumulation of degree-days. Data were submitted to ANOVA and Tukey test at 5% significance level. It was obtained in the summer pruning the reduction in duration of the phenological stages of 'Niagara Rosada' grafted on the rootstock 'Ripária Traviú'. The longer duration of the cycle and accumulation of degree-day of 'Niagara Rosada' cultivar were obtained with the rootstocks 'IAC 572' and 'IAC 313' pruning in the winter.

Análise da presença de vírus em alho semente da segunda e quarta gerações, produzidos por termoterapia e cultura de tecido

The garlic (Allium sativum L.) can be naturally infected by a complex of filamentous viruses belonging to the genera Potyvirus, Carlavirus and Allexivirus. Accumulation of these viruses occurs especially by vegetative propagation through cloves. As the cultivated garlic plant does not produce true seed worldwide, virus-free plants can only be obtained by tissue culture of stem apices and thermotherapy. Using these techniques, garlic seeds were produced at the School of Agricultural Sciences - UNESP, Botucatu, and evaluated by RT-PCR for the presence of potyvirus, carlavirus and allexivirus. In the second generation of microcloves propagated in a greenhouse, 6.6% infection was detected, only by allexivirus. In the fourth generation, however, there was 60% incidence by allexivirus, 35% by potyvirus and all negative by carlavirus. The high rate of infection by allexivirus may be related to the greater difficulty of removing the species of viruses belonging to this genus, as observed by other authors, and also based on the infection and transmission of the virus by the mite, Aceria tulipae, during the storage of bulbs from one year to the other. The garlic at the fourth generation corresponds to cloves weighed less than 1 gram and not selected for commercial multiplication. Selection for the size of cloves has a positive effect on the choice of cloves with lower rates of viral infection, as the technique of thermotherapy and tissue culture do not eliminate the virus completely. Results also emphasize the need of fumigation for the garlic seed stored from one year to the other in order to prevent the transmission of allexivirus during storage.

Caracterização do gene vip3A e toxicidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja

O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por Western blot. Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.

Propagação do pinhão manso por estaquia, enxertia e sementes

Pós-graduação em Agronomia - FEIS

Propagação de variedades de caramboleira por estaquia herbácea

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Clonagem de canistel por estaquia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise técnica e econômica das etapas de produção de mudas de eucalipto

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento da antera, microsporogênese e microgametogênese em Juncus L. (Juncaceae, Poales)

O presente trabalho teve o objetivo de estudar o desenvolvimento da antera, incluindo a microsporogênese e microgametogênese, em Juncus micranthus, J. microcephalus e J. tenuis var. dichotomus avaliando caracteres que possam auxiliar na delimitação infragenérica de Juncus e compará-los àqueles disponíveis para o gênero e outras Juncaceae e famílias ciperídeas em Poales. As três espécies de Juncus estudadas apresentaram características comuns como desenvolvimento da parede da antera do tipo monocotiledôneo, endotécio uniestratificado e com espessamento em espiral, camada mediana uniestratificada e efêmera, tapete secretor com células uninucleadas e grãos de pólen dispersos em tétrades tetraédricas, trinucleadas. Como caracteres inéditos no gênero foram observados, apenas em J. microcephalus, grãos de amido no citoplasma das células do endotécio e da célula vegetativa do grão de pólen, em estágio anterior ao desenvolvimento de espessamento no endotécio. A maioria das características embriológicas aqui observadas assemelha-se ao conhecido para a gênero em Juncaceae, corroborando a uniformidade embriológica da família dentre as ciperídeas em Poales

Diversidade de aranhas em Mimosa bimucronata(DC.) Kuntze (Fabaceae: Mimosoideae): efeito da arquitetura da planta e implicações da predação em interações tri-tróficas

O presente estudo teve como objetivo determinar a diversidade, a composição e a riqueza de espécies de aranhas e suas relações com as diferentes fenofases de Mimosa bimucronata. Visou também comparar a abundância de aranhas entre ramos com inflorescência e com frutos, e investigar se os níveis de infestação de Acanthoscelides schrankiae são reduzidos ou intensificados (alta taxa de predação intraguilda) devido à presença de aranhas. Foram coletadas 777 aranhas, distribuídas em 140 morfoespécies, com uma grande proporção de indivíduos jovens (ninfas). No total, treze famílias de aranhas foram identificadas, sendo que a família Araneidae foi a mais representativa em número de indivíduos coletados e em número de morfoespécies (riqueza). Outras famílias bem representativas foram Anyphaenidae, Thomisidae, Salticidae e Theridiidae. Quando as proporções de aranhas encontradas em ramos com inflorescência e com frutos foram comparadas, não se constatou diferença significativa, mostrando que o tipo de ramo não afetou a abundância das aranhas. No entanto, os estágios fenológicos parecem ter influenciado a riqueza e a diversidade em uma das áreas, uma vez que correlações positivas significativas foram encontradas entre a riqueza e diversidade de morfoespécies e os valores mensais da fenologia reprodutiva. Assim, é possível que o surgimento do conjunto das estruturas reprodutivas, como ramos com botões, flores e frutos, aumentou a complexidade estrutural do habitat, interferindo significativamente na riqueza e diversidade de aranhas. Quando o número médio de orifícios deixados por parasitóides e bruquídeos foi comparado entre áreas, tipos de agregados de frutos (com e sem a presença de teias de aranhas), e considerando a interação entre os fatores, constatou-se diferença significativa apenas entre as áreas, ... (Resumo completo, clicar acesso eletrônico abaixo)

A família celastraceae na reserva biológica municipal da Serra do Japi, Jundiaí, SP

This is a floristic survey Celastraceae in the Reserva Biológica Municipal da Serra do Japi, Jundiaí, SP. It belongs to a major project “Florística vascular da Reserva Biológica Municipal da Serra do Japi, Jundiaí, SP”, in order to create a floristic list of the collected species, likewise to obtain new data for future managements. Seven species, belonging to four genera, were collected: Maytenus, with four species, Hippocratea, Peritassa and Pristimera, with one species each. Identification keys for genera and species, descriptions, illustrations and comments about geographical distributions, fenology and diagnosis characters are provided