Selection of bacterial flora in the Chlortetracycline treated oil sardine (Sardinella longiceps), Indian mackerel (Rastrelliger kanagurta) and prawn (Metapenaeus dobsoni) during ice storage

The native flora of oil sardine and mackerel consisting of Pseudomonas spp; Moraxella spp., Acinetobacter spp. and Vibrio spp. underwent significant changes during ice storage. At the time of spoilage, Pseudomonas spp. were predominant. CTC treatment significantly reduced the Pseudomonas spp. in the initial stages of storage; but later Pseudomonas spp. reasserted and constituted the bulk of the spoilage flora. In prawn, the native flora was comprised of Pseudomonas spp., Acinetobacter spp., Moraxella spp. and Vibrio spp. At the time of spoilage a heterogeneous flora, consisting of Pseudomonas spp; Moraxella spp. and Acinetobacter spp. predominated. CTC treatment significantly changed the flora of prawns. During spoilage, Pseudomonas predominated in CTC treated prawns.

Biochemical changes of fish fingers held at frozen storage

The frozen storage characteristics of fish fingers made out of two different species, differing in lipid content for a period of six months are outlined. The study reveals that the lipid content of the fish meat used for making fish fingers influences the storage pattern in terms of the chemical parameters like peroxide value, thiobarbituric acid value and free fatty acids. The introduction of monosodium glutamate has improved the flavour of the fish fingers. Further, the application of batter on the fish fingers imparted some protective effect in the case of semi-fatty fish.

Studies on frozen storage of ghol (Pseudosciaena diacanthus)

Investigations were carried out on the frozen storage of ghol (Pseudosciaena diacanthus) in the form of fillets. The results indicated that ghol fillets stored at -l8°C remained in a highly acceptable condition up to 20 weeks. However, after this stage, the acceptability steeply declined.

Bacterial flora of EDTA treated oil sardine (Sardinella longiceps), Indian mackerel (Rastrelliger kanagurta) and prawn (Metapenaeus dobsoni) in ice storage

The native flora of fresh oil sardine and mackerel consisted mainly of Pseudomonas spp., Moraxella spp., Acinetobacter spp. and Vibrio spp. During spoilage in ice, nearly 75% of their bacterial flora belonged to Pseudomonas spp. alone. But Na sub(2) EDTA treatment reduced the proportion of Pseudomonas spp. considerably and the major bacterial groups at the time of spoilage were Moraxella spp. and Acinetobacter spp. In the case of fresh prawn, the native flora was constituted by Pseudomonas spp., Moraxella spp., Acinetobacter spp. and Vibrio spp. At the time of spoilage of prawn in ice, Moraxella spp. and Acinetobacter spp. predominated, together constituting 74% of the total population. Na sub(2) EDTA treatment did not alter significantly the spoilage flora of prawns. Moraxella spp. and Acinetobacter spp. accounted for 86% of the spoilage flora in ice storage of Na sub(2) EDTA treated prawns.

Ice storage studies of Kati (Pellona sp.)

Studies were carried out on the effect of ice storage on the composition of kati (Pellona sp.). On the basis of biochemical, bacteriological and organoleptic valuations, it was observed that kati can be stored in ice for a period of 9 days without appreciable loss in overall quality.

Studies on frozen storage of minced fish from threadfin bream

Minced fish prepared from threadfin bream (Nemipterus japonicus) was frozen as blocks, packed in polythene lined waxed cartons and stored at -23°C. The changes taking place during storage were followed. There was good correlation between the organoleptic quality, extractability of protein, cook drip loss and weight loss on thawing. The frozen minced fish was acceptable up to 28 weeks under frozen storage.

Denaturation of Labeo rohita (Rohu) actomyosin on frozen storage: preventive effect of carbohydrates

The preventive effect of sucrose and glucose on the denaturation of frozen rohu actomyosin at -20°C for 7 weeks was examined using an in vitro test model. The rate of denaturation was followed by estimating percentage salt extractability, Ca¹²+ ATPase activity and the clearing response test. Sucrose and glucose showed cryoprotective action for all concentration of actomyosin. Higher actomyosin concentration was preserved better than lower concentration. Post-rigor actomyosin was preserved to a greater extent than pre-rigor actomyosin. Correlation between percentage salt extractability and enzyme activity could not be observed in all samples of frozen actomyosin studied.

Packaging and storage studies of dried lizard fish

Packaging and storage studies of salted and dried lizard fish (Saurida sp.) have been conducted using different synthetic films like low density polyethylene (LDPE) of different gauges, high density polyethylene (HDPE) of 200 gauge, polyvinylidene chloride (PVDC) coated 400 MXXT cellophane, 100 gauge polypropylene (PP) and paper laminate of 100 gauge polythene. The films found most effective in the preliminary studies were subsequently used for packaging and storage of dried fish at atmospheric and lower temperature and humidity conditions for confirming their suitability under these conditions. Polyethylene films of higher gauges showed better results under both sets of conditions. PVDC coated cellophane film also performed satisfactorily under the latter conditions which under the former condition got easily attacked by insects. Lower temperature and humidity conditions in general enhanced the storage life of the dried product.

Denaturation of Labeo rohita (rohu) actomyosin on frozen storage: preventive effect of dicarboxylic acids

The preventive effect of fumarate, maleate, tartrate and oxalate on the denaturation of frozen rohu actomyosin at -20°C in 0.7 M KCl for 7 weeks was examined using an in vitro test model. The rate of denaturation was followed by estimating percentage salt extractability, Ca²+ ATP-ase activity and the clearing response test. Fumarate, maleate and tartrate showed cryoprotective effect for higher concentration of pre-rigor rohu actomyosin of 10 mg/ml and 20 mg/ml. At actomyosin concentration of 6 mg/ml, maleate and tartrate showed some preventive effect whereas fumarate enhanced denaturation. Oxalate showed poor cryoprotective action. Post-rigor rohu actomyosin was preserved frozen without denaturation to a greater extent than pre-rigor actomyosin.

Studies on frozen storage characteristics of sole fish Cynoglossus macrolepidotus

Fresh sole fish (Cynoglossus macrolepidotus) was quick frozen at -40°C and stored at -18°C. Shelf life was evaluated by following biochemical, bacteriological and organoleptic changes occurring during storage. Rapid decrease was noted in the water extractable nitrogen and salt soluble nitrogen fractions. Samples of frozen sole fish remained in acceptable condition for 20 weeks.

Preparation and storage of cutlet from low-priced fish

Cutlets have been prepared from the minces of lizard fish (Saurida tumbil), threadfin bream (Nemipterus japonicus), jew fish (Johnius dussumieri) and miscellaneous fish. The storage characteristics of cutlet (both raw and flash fried) at 4°C, -8°C and -20°C were studied. Cutlets prepared from the minced lizard fish showed the highest acceptability. Flash fried cutlets were found to be superior in quality compared to raw cutlets. The raw cutlet had storage life of 6 days, 11 weeks and 19 weeks at 4°C, -8°C and -20°C respectively and flash fried cutlets had a shelf life of 22 weeks at -20°C.

Changes in the muscle of three Indian major carps during frozen storage

Chunks of Labeo rohita, Cirrhinus mrigala and Catla catla wrapped in polythene film were stored at -8 to -10°C in the freezer cabinet of the refrigerator. It was found that L. rohita and C. mrigala were acceptable up to 33 days and C. catch up to 35 days. Total volatile base nitrogen, free fatty acids and degree of sponginess of the samples showed increasing trend during frozen storage.

Quality deterioration in tilapia during storage in refrigerated brine

During storage of Tilapia nilotica in refrigerated brine at 4°C, the whole (ungutted) fish had higher levels of free fatty acid and spoiled faster than the gutted fish. The shelf life of whole fish was 16 days and that of gutted 28 days; these values are, at least, as good as those reported for ice storage. Flavour of the cooked fish appeared to be the quahty-hm1tmg factor reducing the shelf-life of gutted tilapia to 28 days even though the raw gutted fish was judged acceptable, after 31 days retaining 65-70% freshness.

Effectiveness of EDTA dips on the shelf-life of oil sardine (Sardinella longiceps), mackerel (Rastrelliger kanagurta) and prawn (Metapenaeus dobsoni) in iced storage

Fresh oil sardine, mackerel and prawn were dipped in 0.1% and 1% solutions of Na sub(2)EDTA, and stored in ice. Their storage-life was assessed by bacteriological, chemical and sensory methods. Even though EDTA treatment controlled the increase in bacterial counts and reduced TMA and TVBN production in oil sardine and mackerel, the consequent beneficial effect was not realised because of the deterioration of fat in these fishes, leading to rancidity. But, for prawn stored in ice, a dip in 1% solution of Na sub(2)EDTA enhanced the shelf-life by at least 8 days over the untreated control. EDTA absorbed by the muscle of fish and prawn during dip in Na sub(2)EDTA solution is not completely removed during their iced storage for 25 days.