996 resultados para amostras de folha
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O trabalho investiga os projetos editoriais e gráficos da Folha de S. Paulo produzidos desde a década de 1970, de modo a tentar compreender a evolução histórica recente do veículo, além do amadurecimento de seu conceito de jornalismo e de sua visão como empresa, os quais o alçaram à condição de jornal mais importante do país, posição que raramente perdeu e ainda mantém. Com ferramentas metodológicas emprestadas de Mikhail Bakhtin, a autora demonstra que, apesar das estabilidades estruturais desse jornal enquanto gênero discursivo se ampararem em elementos como conteúdo temático, traços formais e estilo verbal, esses mesmos elementos seriam sutilmente transformados ao longo dos anos, de modo a adaptar continuamente o veículo ao universo mutável que o circunda, constituído pelos leitores, anunciantes, governos, o desenvolvimento tecnológico e o próprio conceito de jornalismo. A pesquisadora mostra o quanto a Folha de S. Paulo precisou se modificar para enfrentar a concorrência dos outros jornais, da televisão e, em especial, da internet, que impôs um novo tipo de tratamento à informação. E como, nesse percurso, o jornal conseguiu consolidar sua visão de mundo e sua noção particular de informação como mercadoria
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Chlamydophila psittaci is a bacterium that causes respiratory or systemic disease in birds and humans. Owing to the risk of transmission from asymptomatic birds to humans, the objective of this study was to detect the presence of Chlamydophila spp. in asymptomatic birds. Four hundred and three fecal samples or cloacal swabs were collected from domestic, wild or exotic birds. The 403 samples were examined by real time PCR specific for the 16S subunit of rRNA gene using SsoFastEvaGreen®SupermixTM (Bio-Rad) and melting curve analysis. Hemi-nested PCR specific for the OMP-A gene, accomplished in real-time PCR positive samples, was followed by sequencing of the amplified fragments to determine the genotype of C. psittaci. Real-time PCR was positive in 17 (4.21%) samples. Hemi-nested PCR revealed positivity in two samples previously positive by real-time PCR. Sequencing of the fragment amplified by hemi-nested PCR allowed for the identification of genotype A of C. psittaci in one sample. The results of this experiment show that the real-time PCR targeting the 16S rRNA gene followed by melting curve analysis can be used for diagnosis of Chlamydophila sp. in fecal samples of asymptomatic birds. The classification of the Chlamydophila species and the genotype of C. psittaci must be accomplished by PCR targeting the ompA gene and sequencing of the amplified fragments.
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Effects of bentazon, metolachlor, trifluralin, imazethapyr, imazethapyr+lactofen, haloxyfop-methyl, glyphosate and chlorimuron-ethyl at rates of 2 and 10 times the equivalent commercial dose on soil microbial activity was evaluated in soil samples extracted from a field never treated before. Global soil microbe respiration, estimated by CO2 production at 2, 4, 8, 12, 16, 20, 24 and 28 days of soil incubation and enzymatic activities (dehydrogenase and fluorescein diacetate hydrolysis) at 8 and 28 days were used as bioindicators. Bentazon and mixture imazethapyr+lactofen at the highest rate and haloxyfop-methyl at both rates, inhibited soil respiration although with differences in timing and duration. None of the herbicides affected FDA hydrolysis. Dehydrogenase activity was inhibited at 8 days of incubation with bentazon and imazethapyr at high rates but it was stimulated by metolachlor and imazethapyr at low rate and glyphosate at the highest rate. Herbicide effects on soil microbial activity was detected with higher sensitivity by global soil microbe respiration and dehydrogenase activity than by FDA hydrolysis. Only dehydrogenase activity and soil respiration estimations at 8 days of soil incubation had significant correlation. Results indicated the need of multiple estimations when evaluating herbicides effects on soil microbiota
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Pós-graduação em Biologia Geral e Aplicada - IBB
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Pós-graduação em Microbiologia Agropecuária - FCAV
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Pós-graduação em Química - IQ
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Pós-graduação em Química - IQ
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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There is little research on the practices of sanitary inspection in the chain of production of vegetables to the consumer, especially those eaten raw, they are liable to serve microorganism such as bacteria, fungi and parasites, contributing to possible health hazards. The aim of this study was to assess qualitatively contamination by parasites and / or commensals of medical interest in lettuce leaves (Lactuca sativa) fresh market in the municipality of Quata Sao Paulo. A total of 15 random samples were analyzed every other day of the three different places that sell vegetables a grocery store, a supermarket and a vegetable garden during the month of May 2011. The parasites and / or commensals found in lettuce were Entamoeba coli (67%), Entamoeba histolytica (20%), Giardia sp (13%) and Ascaris lumbricoides (7%). The analysis showed the presence of parasites and / or commensals in all samples, except in the cultivated garden which showed poor sanitary conditions, probably due to contamination in the shipping and handling by third parties in supermarket and grocery store. The parasite monitoring sanitary conditions of vegetables sold in urban environments becomes relevant for preventive measures to avoid the continued parasitic cycle and possible future health complications.
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The objective of this study was to analyze different intensities of soil sampling for accuracy in geostatistical analysis and interpolation maps for precision agriculture in the sugarcane area. Soil samples were collected at two regular grids at a depth of 0.00 to 0.20m for granulometric analysis (area 1) and soil fertility (area 2). We compared soil sampling intensities: 208, 105, 58 and 24 points in Area 1 and 206, 102 and 53 points in Area 2. The data were submitted to descriptive analysis and geostatistics. The variograms constructed with 105 points didn't differ from variograms with 208 points, which doesn't occur for 58 and 24 points. The increase of sampling interval and reducing the number of points promote greater error in kriging. Samples with more than 100 points per area did not result in significant improvements in the error of kriging, or differed in the amount of fertilizer applied to the field.
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Pós-graduação em Zootecnia - FMVZ
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Pós-graduação em Agronomia (Produção Vegetal) - FCAV
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)