Compostagem de resíduo sólido de abatedouro avícola

The great demand for animal protein was responsible for the increase on the broilers production and hence, the generation of waste from the poultry slaughter was increased as well, which in turn, propelled the development of techniques that allow the reuse and recycling of these wastes. The objective of this study was to evaluate the efficiency of composting on the treatment and recycling of solid waste from poultry slaughterhouse. The solid waste was from a commercial poultry slaughterhouse and was composed of viscera, muscle, fat, bone, blood and feathers that was mixed with a source of carbon, rice husk. Initially, a windrow with a volume of 1.5m(3) was built, and then some parameters were monitored: temperature, total solids (TS), volatile (VS), N, P, K, organic carbon (C), composting organic matter (COC), organic matter resistant to composting (MORC), chemical oxygen demand (COD), mass and volume of the windrow, most probable number (MPN) of total and fecal coliforms, as well as their reductions during the process. The maximum temperature reached in the center of the windrow was 53.3 degrees C (weekly average) since reductions of weight of TS and VS and volume during the pre-composting were 36.1, 44.3 and 23.3%, respectively and during the composting process was 21.8, 23.8 and 4.4%. The low volume reduction can be associated with high concentrations of MORC (40.1%) which can be mainly related to the quality of the carbon source. The process produced satisfactory total reductions of TS, VS and volume that were respectively, 50.1, 57.5 and 26.7%. However reductions were observed in 43% of amount of nitrogen in the final compound. Despite reductions in nitrogen content, composting proved to be an effective method in the treatment of solid waste from poultry slaughterhouse.

Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1 M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1 M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.

Contribution of different segments of the gastrointestinal tract to digestion in growing Saanen goats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Multiphase feeding program for broilers can replace traditional system

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Protein and energy requirements of castrated male Saanen goats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mineral requirements for growth and maintenance of F-1 Boer x Saanen male kids

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Early weaning and concentrate supplementation strategies for lamb production on Tifton-85 pasture

The influence of early weaning and concentrate supplementation strategies on sward characteristics, forage chemical composition and lamb productivity were evaluated in four production systems on Tifton-85 pasture: suckling lambs not supplemented until slaughter; suckling lambs supplemented with concentrate in creep feeding until slaughter; early-weaned lambs not supplemented until slaughter; and early-weaned lambs supplemented with concentrate until slaughter. Structural, morphological and productive characteristics of pasture were measured. The forage was chemically analyzed to estimate its composition. Lambs average daily gain and productivity were calculated. Sward height, forage and morphological components mass were lower in systems without weaning. Forage production was higher in systems with supplementation. Higher levels of neutral and acid detergent fiber were observed in forage ingested by lambs in creep feeding and by weaned and unsupplemented lambs. Average daily gain was higher for lambs in creep feeding (275 g/d) and lower for the weaned and unsupplemented animals (57 g/d). Productivity was higher for weaned and supplemented lambs (21 kg lamb body weight, BW gain/ha/d). Lower productivity was observed in systems without supplementation (5 kg lamb BW gain/ha/d on average). Ewes modify the sward conditions improving the pasture characteristics and the quality of forage produced. Changes in sward conditions affect the chemical composition of forage ingested by lambs. Early weaning may be an alternative to maximize pasture utilization in small areas. Concentrate supplementation may increase lamb performance and productivity in grazing systems. If the objective is to improve lamb individual performance, creep feeding should be used.

Energy requirements for growth of pubertal female Saanen goats

Previous research on energy requirements of female Saanen goats, using the factorial approach, has not considered the specific requirements for maintenance and growth during the pubertal phase. Thus, the purpose of this study was to estimate energy requirements for maintenance (Trial 1) and growth (Trial 2) of non-pregnant and non-lactating female Saanen goats at the pubertal phase from 30 to 45 kg. In Trial 1, the net energy requirements for maintenance (NEm ) were estimated using 18 female Saanen goats randomly assigned to three levels of intake: ad libitum, and 70% and 40% of ad libitum intake. These animals were pair-fed in six slaughter groups, each consisting of one animal for each level of intake. In Trial 2, the net energy requirements for growth (NEg ) were estimated using 18 female Saanen goats, which were fed ad libitum and slaughtered at targeted BW of 30, 38 and 45 kg. The NEm was 52 kcal/kg(0.75) of BW. The NEg increased from 3.5 to 4.7 Mcal/kg of BW gain as BW increased from 30 to 45 kg. Our results suggest that the guidelines of the major feeding systems for the entire growth phase may not be adequate for females at pubertal phase.

Características da carcaça e alometria dos tecidos de cabritos F1 Boer × Saanen

Com o objetivo de avaliar as características de carcaça e a alometria dos cortes comerciais e dos tecidos de cabritos F1 Boer × Saanen, 35 animais foram abatidos ao atingirem 5, 10, 15, 20 e 25 kg de peso vivo (PV). A dieta dos animais foi composta de leite de vaca nos primeiros 49 dias e ração à vontade do sétimo dia até o abate. Os cortes foram obtidos após o resfriamento da carcaça e a perna foi dissecada em músculo, osso e gordura. O PV teve efeito linear decrescente no rendimento de carcaça fria e na área de olho-de-lombo por kg de carcaça. Os pesos de perna, paleta e pescoço em relação à carcaça fria decresceram linearmente, mas houve efeito quadrático sobre o rendimento de costelas e lombo. O crescimento de paleta, pescoço e perna foi isométrico (b=1) ao do corpo, enquanto o das costelas e do lombo foi mais lento (b¹ 1). Os músculos da perna cresceram igualmente, a gordura mais lenta e os ossos mais rapidamente que a perna, enquanto o desenvolvimento da gordura subcutânea foi mais tardio que o da intermuscular. Para obtenção de carcaça de 8 a 11 kg com rendimento superior a 44%, boa proporção de músculo e gordura com menor perda durante o resfriamento, recomenda-se abater os animais com PV entre 20 e 25 kg, mas, se o objetivo for carcaça de menor peso, o abate dos animais deve ser feito ao final do aleitamento com aproximadamente 10 kg de peso corporal.

Cortisol sanguíneo e qualidade de carcaças de frangos abatidos pelo método halal ou com insensibilização por eletronarcose

Pós-graduação em Medicina Veterinária - FCAV

Predição genômica para o peso ao sobreano via norma de reação em bovinos de corte

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Recuperação de biomassa bacteriana produzida no tratamento de efluente industrial

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stunning method in broiler chickens meat processing

Quality is a variable concept, which involves many factors, depending on the consumer market. In meat production, the concern with environmental aspects, animal welfare and the health and safety of workers is increasing. This work studied the effect of controlled atmosphere stunning of broilers on meat features and biochemical parameters for stress. Cobb broilers were stunned by electrical stunning and by controlled atmosphere with 70% CO2 and 70% CO2 + 30% Argon. After stunning, serum levels of glucose, lactate and corticosterone were compared with those of broilers at rest, immediately before transportation and slaughter and after 12 h of feed withdrawal (control group). At slaughter, blood volume drained during bleeding was not different for the stunning methods tested, ranging from 3.3 to 3.4% birds weight. This finding was important to demonstrate that gas stunning was not responsible for the animals′ death. Final pH in breast (6.1 to 6.2) and thigh (6.3 to 6.5) also did not vary among the different stunning methods (P > 0.05). Lightness (L = 60.55) and redness (a = +8.94) values found for breasts from electrical stunning showed that they were darker and redder (P < 0.05), probably due to changes in blood pressure. Glucose and corticosterone levels were not different between gas stunned birds (302.45 to 315.7 mg/dl and 55.71 to 72.49 ng/ml respectively) and birds at rest (305.95 mg/dl and 50.65 ng/ml) (P > 0.05). These stress indicators were higher (337.65 mg/dl for glucose and 104.13 ng/ml for costicosterone) when electrical stunning was used (P < 0.05). Lactate concentrations were lower (5.4 mmol/l) for birds at rest (P < 0.05) but not different for all stunning methods tested (7.3 to 8.1 mmol/l; P > 0.05). These results show that serum glucose may be used as a stress indicator in birds, with the advantage of being a quick and cheap biochemical test. Gas stunning favored birds′ management during slaughter and so reduced workers′ effort and injury hazard and the amount of feces and dust in the room. To make this method available for a large scale process, adjustments in equipments will be necessary to avoid delays in the processing line.

Extrinsic factors affecting water absorption in chilling of broiler chicken carcasses

Brazil occupies an outstanding position as a producer and exporter of chicken meat, and the maintenance and expansion of this position require a constant evolution, especially in variables which determine quality. An important quality parameter of poultry meat is the amount of water absorbed by the carcass during processing. In Brazil, carcasses chilling is done by immersion in chilled water. In this process, the carcass is rehydrated and the water lost during transport and initial operations is replaced. At this stage, some care is needed to prevent the absorption of water upper than the level allowed by Brazilian law. This project aimed to evaluate extrinsic factors that can influence the absorption of water by the chicken meat. For this, 144 Cobb chickens divided into 24 groups of six birds were used. At 42 days of age, one chicken of each group, with weight ranging up to 10% more or less from the average of the group, was slaughtered in an experimental pilot scale abattoir where slaughter procedures were conducted under strictly controlled conditions. The chilling procedure was performed following a completely randomized design with factorial arrangement 3x2, where the factors were: three temperatures in the first section of the chilling system (4, 10 and 16ºC) and two degrees of water hardness (hard and soft water), with six treatments and four replications. Brazilian law provides that the water temperature in the first section of the chiller must not be higher than 16ºC, and the length of the carcasses in this section shall not exceed 30 minutes. All carcasses remained in the first section of the chiller for 30 minutes and then were transferred to another tank with water at 4ºC, remaining there until reaching 7ºC. The carcasses were weighed before and after chilling, to evaluate the percentage of water absorbed. The water absorption was influenced by the initial temperature of the water in the chiller and by the water hardness. When initially immersed in water at 4ºC, carcasses water absorption averaged 2.70%, a significantly lower absorption than the values found for the carcasses that were initially immersed in water at 16ºC, 3.83% (p<0.05). The carcasses immersed in water at 10ºC had mean water absorption of 3.66%, not differing from the means observed in the other two treatments (p>0.05). In hard water, the average water absorption was 2.46% and, in soft water, 4.33% (p<0.05). In all treatments, the water absorption did not exceed the limit established by Brazilian legislation, which is a maximum of 8%. This information is important to control the absorption of water by carcasses in chicken meat processing, preventing consumers from being harmed.

Accurate adjustment of feed formulation for broiler chickens on performance and lipid composition of meat

Most consumers consider the fat of chicken meat undesirable for a healthy diet, due to the high levels of saturated fatty acids and cholesterol. The purpose of this experiment was to investigate the influence of changes in dietary metabolizable energy level, associated with a proportional nutrient density variation, on broiler chickens performance and on the lipid composition of meat. Males and females Cobb 500 broilers were evaluated separately. Performance evaluation followed a completely randomized design with factorial 6x3 arrangement - six energy levels (2,800, 2,900, 3,000, 3,100, 3,200 and 3,300 kcal/kg) and three slaughter ages (42, 49 and 56 days). Response surface methodology was used to establish a mathematical model to explain live weight, feed intake and feed conversion behavior. Total lipids and cholesterol were determined in skinned breast meat and in thigh meat, with and without skin. For lipid composition analysis, a 3x3x2 factorial arrangement in a completely randomized design - three ration’s metabolizable energy levels (2,800, 3,000 and 3,300 kcal/kg), three slaughter ages (42, 49 and 56 days) and two sexes - was used. The reduction in the diet metabolizable energy up to close to 3,000 kcal/kg did not affect live weight but, below this value, the live weight decreased. Feed intake was lower when the dietary energy level was higher. Feed conversion was favored in a direct proportion to the increase of the energy level of the diet. The performance of all birds was within the range considered appropriate for the lineage. Breast meat had less total lipids and cholesterol than thigh meat. Thigh with skin had more than the double of total lipids of skinned thigh, but the cholesterol content did not differ with the removal of the skin, suggesting that cholesterol content is not associated with the subcutaneous fat. Intramuscular fat content was lower in the meat from birds fed diets with lower energy level. These results may help to define the most appropriate nutritional management. Despite the decrease in bird’s productive performance, the restriction of energy in broiler chickens feed may be a viable alternative, if the consumers are willing to pay more for meat with less fat.