Vida y obra del artista canario Carlos Morón

[ES] El presente trabajo de investigación responde a la demanda social de revitalizar y dar a conocer a las generaciones actuales la polifacética personalidad artística de Carlos Morón. Para ello, se pensó que lo más conveniente era la realización de un trabajo monográfico sobre su peculiar biografía y heterogénea obra que abarcó el retrato, la pintura mural, la de historia, el paisaje, bodegones, costumbrista, escenografía, escultura y el grabado.

Aspectos reproductivos y acondicionamientos de reproductores de Haliotis tuberculata coccinea (Reveé, 1846)

Programa de Doctorado: Acuicultura: producción controlada de animales acuáticos

Optimización del cultivo de diatomeas bentónicas para el cultivo de post-larvas de abalón ("Haliotis tuberculata coccinea" Reeve, 1846)

[EN] Diatom cell quantity and their biochemical composition vary among species and are greatly affected by harvest stage or culture conditions. Biometric parameters, growth, attachment capacity and variations in biochemical composition of four species of benthic diatoms (Amphora sp., Navicula incerta, Nitzschia sp. and Proschkinia sp.) were studied. For biochemical analysis the diatoms were harvested at different stages, in log and stationary phase of growth. The culture conditions were identical for all the experiments, benthic diatoms were cultured during 7 days in F/2 medium at 28.5 ± 1.4 ºC, at different original inoculating densities (50000, 100000, and 250000 cell mL-1), under continuous light of 5403 ± 649 Lux provided by cool white fluorescent lighting. The cultures were neither aerated nor agitated. These results show that the specific density of 10000 cell mL-1 was the best for weekly production: Proschkinia sp. reached the highest cell density of 5.81 x 106 cells mL-1 and Amphora sp. had the highest cell attachment capacity with 12000 cell mm-2, in stationary phase of growth. Protein and lipid content were higher in log phase than in stationary phase for the four diatoms. Amphora sp. in log phase of growth had the highest lipid content of 9.74% dry weight (DW). Polyunsaturated fatty acid (PUFA) content ranged from 23.25% to 38.62% of the total fatty acids (TFA), and the four diatoms tested were richer in n-3 PUFA than in n-6 PUFA. All the diatoms had significant quantities of 20:5n-3 (EPA) ranging between 12.69% and 17.68% of TFA. Benthic diatoms play an important and critical role in abalone culture as they are the principal food source of abalone post-larvae. Therefore, it is necessary to improve diatom quantity and quality to optimize post-larval nutrition and the consistency of production, resulting in an increase in growth and survival of abalones.

Con la venia de Carlos III. El castigo ejemplar de Olavide, consecuencia de la venganza de Grimaldi contra el conde de Aranda

[ES] Los inquisidores castigaron a Pablo de Olavide en 1776, pero fueron Grimaldi, ministro de Estado, y Carlos III los que le eligieron como castigo ejemplar para responder a la agitación política provocada por el conde de Aranda en su deseo de volver al poder. Como no podían tocar a un grande de España como Aranda, se vengaron en su protegido Olavide y lo llevaron a las cárceles secretas de la Inquisición. Todo se acordó con Su Majestad. [EN] Inquisitors punished Pablo de Olavide in 1776, but Grimaldi, Minister of State, Inquisitors punished Pablo de Olavide in 1776, but Grimaldi, Minister of State, and Charles III were the ones who chose his case as an exemplary punishment to respond to the political turmoil caused by the Count of Aranda after an attemp to return to power. As they could not punish a grandee of Spain such as Aranda, they took revenge on his protégé Olavide and took him to the secret prisons of the Inquisition. Everything was agreed with His Majesty.

Transacciones, delegación o unilateralidad. Un análisis de los equilibrios de poder en las relaciones ejecutivo-legislativo durante los primeros gobiernos de Álvaro Uribe en Colombia y Carlos Saúl Menem en Argentina.

El presente trabajo analiza las relaciones entre poder ejecutivo y legislativo en los sistemas presidenciales, concentrándose específicamente en los casos de los primeros gobiernos de Álvaro Uribe Vélez en Colombia [2002-2006] y de Carlos Saúl Menem en Argentina [1989-1995]. Lo hace desde de una perspectiva analítica denominada neomadisoniana. Ubicada bajo el paraguas del neoinstitucionalismo, ésta utiliza como punto de partida el pensamiento de los padres fundadores de la democracia americana, particularmente el de James Madison, concentrándose en el estudio de como los arreglos institucionales constriñen el comportamiento de los actores a través del establecimiento de incentivos mediante los que se distribuyen distintos recursos de poder, proporcionando, además, una estructura estable, aunque no necesariamente eficiente, para la interacción humana. De este modo, el enfoque posee un particular interés por el análisis de la organización de los gobiernos, ya sea en términos de jerarquía o de transacciones entre actores instituciones [poderes ejecutivo y legislativo] que, de acuerdo al caso, gozan de distintos niveles de simetría y, consecuentemente, de capacidad de impactar sobre el proceso de toma de decisiones. Dentro de este marco, se intentarán identificar en el análisis de los casos patrones de regularidad y diferencia en relación a las características asumidas en el proceso de separación de poderes y el efecto que ejercen sobre él los poderes constitucionales y partidarios de los presidentes.

Stock boundaries of Parapenaeus longirostris (Lucas, 1846) populations in the Italian seas: Development of genomic 2b-RAD markers and biological variation analysis.

This thesis is developed in the contest of Ritmare project WP1, which main objective is the development of a sustainable fishery through the identification of populations boundaries in commercially important species in Italian Seas. Three main objectives are discussed in order to help reach the main purpose of identification of stock boundaries in Parapenaeus longirostris: 1 -Development of a representative sampling design for Italian seas; 2 -Evaluation of 2b-RAD protocol; 3 -Investigation of populations through biological data analysis. First of all we defined and accomplished a sampling design which properly represents all Italian seas. Then we used information and data about nursery areas distribution, abundance of populations and importance of P. longirostris in local fishery, to develop an experimental design that prioritize the most important areas to maximize the results with actual project funds. We introduced for the first time the use of 2b-RAD on this species, a genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Thanks to this method we were able to move from genetics to the more complex genomics. In order to proceed with 2b-RAD we performed several tests to identify the best DNA extraction kit and protocol and finally we were able to extract 192 high quality DNA extracts ready to be processed. We tested 2b-RAD with five samples and after high-throughput sequencing of libraries we used the software “Stacks” to analyze the sequences. We obtained positive results identifying a great number of SNP markers among the five samples. To guarantee a multidisciplinary approach we used the biological data associated to the collected samples to investigate differences between geographical samples. Such approach assures continuity with other project, for instance STOCKMED, which utilize a combination of molecular and biological analysis as well.

Carlos Martí Arís e i suoi eteronimi. Vocazione all'anonimo

Questa ricerca indaga la prospettiva investigativa di Carlos Martí Arís. È stato assunto il campo d’azione da lui prediletto, ovvero l’articolato rapporto che in architettura si instaura tra teoria e pratica, comprensivo delle svariate ricadute nel mondo dell’arte e della produzione umana in genere, che fanno del progetto architettonico un campo disciplinare complesso. La sua figura è però assunta in modo strumentale, come grimaldello per addentrarsi in un articolato ambito culturale, che se da un lato coincide con la sua città, Barcellona, dall’altro la trascende grazie a quei “ponti della conoscenza” che CMA interrottamente ha teso al suo intorno. Ci riferiamo alla sua costruzione teorica destinata a consolidare la storica reciprocità tra Italia e Spagna, le cui tematiche urbane e tipologiche ne sono la base, Milano e Barcellona ne sono gli estremi. Ci riferiamo al suo sguardo sull’esperienza del Movimento Moderno e il relativo tema della residenza. Ci riferiamo alla sua naturale vocazione al silenzio, che si oppone al fragoroso rumore della contemporaneità e affianca la discreta parola del mestiere: un modo per porsi all’ascolto. All’ascolto dell’altro e del mondo. Ci riferiamo, insomma, alla sua idea di architettura intesa come «territorio dissodato da tempi remoti»; come trama di corrispondenze sincroniche tra terre, tempi, fatti, uomini, vicini e lontani: condizione ideale per chi voglia disciogliere il proprio lavoro nei labirintici sentieri del mondo, indifferente al rischio di perdersi nell’oblio. Oggi, in cui il progetto architettonico risulta sempre più spesso veicolo di soggettive e arbitrarie sperimentazioni formali, la lezione di CMA indica una via d’uscita: un mo(n)do condiviso che all’arroganza opponga la discrezione, che persuada a celarsi nella tradizione e a porsi umilmente all’ombra dei maestri. Tradizione e Maestri, Eteronimi e Nomi, complementarità a cui CMA affida il suo progetto di anonimato, sovrapersonale e ostinatamente teso a rilevarne le relazioni inedite.

The distribution of E-cadherin expression in listeric rhombencephalitis of ruminants indicates its involvement in Listeria monocytogenes neuroinvasion

AIM: To investigate the expression of E-cadherin, a major host cell receptor for Listeria monocytogenes (LM) internalin A, in the ruminant nervous system and its putative role in brainstem invasion and intracerebral spread of LM in the natural disease. METHODS: Immunohistochemistry and double immunofluorescence was performed on brains, cranial nerves and ganglia of ruminants with and without natural LM rhombencephalitis using antibodies against E-cadherin, protein gene product 9.5, myelin-associated glycoprotein and LM. RESULTS: In the ruminant brain, E-cadherin is expressed in choroid plexus epithelium, meningothelium and restricted neuropil areas of the medulla, but not in the endothelium. In cranial nerves and ganglia, E-cadherin is expressed in satellite cells and myelinating Schwann cells. Expression does not differ between ruminants with or without listeriosis and does not overlap with the presence of microabscesses in the medulla. LM is observed in phagocytes, axons, Schwann cells, satellite cells and ganglionic neurones. CONCLUSION: Our results support the view that the specific ligand-receptor interaction between LM and host E-cadherin is involved in the neuropathogenesis of ruminant listeriosis. They suggest that oral epithelium and Schwann cells expressing E-cadherin provide a port of entry for free bacteria offering a site of primary intracellular replication, from where the bacterium may invade the axonal compartment by cell-to-cell spread. As E-cadherin expression in the ruminant central nervous system is weak, only very locally restricted and not related to the presence of microabscesses, it is likely that further intracerebral spread is independent of E-cadherin and relies primarily on axonal spread.