Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum-free microcarrier process

Human mesenchymal stem cell (hMSC) therapies are currently progressing through clinical development, driving the need for consistent, and cost effective manufacturing processes to meet the lot-sizes required for commercial production. The use of animal-derived serum is common in hMSC culture but has many drawbacks such as limited supply, lot-to-lot variability, increased regulatory burden, possibility of pathogen transmission, and reduced scope for process optimization. These constraints may impact the development of a consistent large-scale process and therefore must be addressed. The aim of this work was therefore to run a pilot study in the systematic development of serum-free hMSC manufacturing process. Human bone-marrow derived hMSCs were expanded on fibronectin-coated, non-porous plastic microcarriers in 100mL stirred spinner flasks at a density of 3×10⁵cells.mL^-1 in serum-free medium. The hMSCs were successfully harvested by our recently-developed technique using animal-free enzymatic cell detachment accompanied by agitation followed by filtration to separate the hMSCs from microcarriers, with a post-harvest viability of 99.63±0.03%. The hMSCs were found to be in accordance with the ISCT characterization criteria and maintained hMSC outgrowth and colony-forming potential. The hMSCs were held in suspension post-harvest to simulate a typical pooling time for a scaled expansion process and cryopreserved in a serum-free vehicle solution using a controlled-rate freezing process. Post-thaw viability was 75.8±1.4% with a similar 3h attachment efficiency also observed, indicating successful hMSC recovery, and attachment. This approach therefore demonstrates that once an hMSC line and appropriate medium have been selected for production, multiple unit operations can be integrated to generate an animal component-free hMSC production process from expansion through to cryopreservation.

Multi-wavelength source using low drive-voltage amplitude modulators for optical communications

A simple and cost-effective technique for generating a flat, square-shaped multi-wavelength optical comb with 42.6 GHz line spacing and over 0.5 THz of total bandwidth is presented. A detailed theoretical analysis is presented, showing that using two concatenated modulators driven with voltages of 3.5 Vp are necessary to generate 11 comb lines with a flatness below 2dB. This performance is experimentally demonstrated using two cascaded Versawave 40 Gbit/s low drive voltage electro-optic polarisation modulators, where an 11 channel optical comb with a flatness of 1.9 dB and a side-mode-suppression ratio (SMSR) of 12.6 dB was obtained.

On the theory of autosoliton propagation in optical fibers guided by in-line nonlinear devices

A theoretical model is developed to describe the propagation of ultra-short optical pulses in fiber transmission systems in the quasi-linear regime, with periodically inserted in-line lumped nonlinear optical devices. Stable autosoliton solutions are obtained for a particular application of the general theory.

Protection schemes for meshed VSC-HVDC transmission systems for large-scale offshore wind farms

This chapter discusses network protection of high-voltage direct current (HVDC) transmission systems for large-scale offshore wind farms where the HVDC system utilizes voltage-source converters. The multi-terminal HVDC network topology and protection allocation and configuration are discussed with DC circuit breaker and protection relay configurations studied for different fault conditions. A detailed protection scheme is designed with a solution that does not require relay communication. Advanced understanding of protection system design and operation is necessary for reliable and safe operation of the meshed HVDC system under fault conditions. Meshed-HVDC systems are important as they will be used to interconnect large-scale offshore wind generation projects. Offshore wind generation is growing rapidly and offers a means of securing energy supply and addressing emissions targets whilst minimising community impacts. There are ambitious plans concerning such projects in Europe and in the Asia-Pacific region which will all require a reliable yet economic system to generate, collect, and transmit electrical power from renewable resources. Collective offshore wind farms are efficient and have potential as a significant low-carbon energy source. However, this requires a reliable collection and transmission system. Offshore wind power generation is a relatively new area and lacks systematic analysis of faults and associated operational experience to enhance further development. Appropriate fault protection schemes are required and this chapter highlights the process of developing and assessing such schemes. The chapter illustrates the basic meshed topology, identifies the need for distance evaluation, and appropriate cable models, then details the design and operation of the protection scheme with simulation results used to illustrate operation. © Springer Science+Business Media Singapore 2014.

Demonstration of nonlinear inverse synthesis transmission over transoceanic distances

Nonlinear Fourier transform (NFT) and eigenvalue communication with the use of nonlinear signal spectrum (both discrete and continuous), have been recently discussed as promising transmission methods to combat fiber nonlinearity impairments. In this paper, for the first time, we demonstrate the generation, detection and transmission performance over transoceanic distances of 10 Gbaud and nonlinear inverse synthesis (NIS) based signal (4 Gb/s line rate), in which the transmitted information is encoded directly onto the continuous part of the signal nonlinear spectrum. By applying effective digital signal processing techniques, a reach of 7344 km was achieved with a bit-error-rate (BER) (2.1×10-2) below the 20% FEC threshold. This represents an improvement by a factor of ~12 in data capacity x distance product compared with other previously demonstrated NFT-based systems, showing a significant advance in the active research area of NFT-based communication systems.

PMD tolerant nonlinear compensation using in-line phase conjugation

In this paper, we numerically investigate the impact of polarisation mode dispersion on the efficiency of compensation of nonlinear transmission penalties for systems employing one of more inline phase conjugation devices. We will show that reducing the spacing between phase conjugations allows for significantly improved performance in the presence polarisation mode dispersion or a significant relaxation in the acceptable level of polarization mode dispersion. We show that these results are consistent with previously presented full statistical analysis of nonlinear transmission appropriately adjusted for the reduced section length undergoing compensation.

DC power line communication based on power/signal dual modulation in phase shift full bridge converters

For intelligent DC distributed power systems, data communication plays a vital role in system control and device monitoring. To achieve communication in a cost effective way, power/signal dual modulation (PSDM), a method that integrates data transmission with power conversion, can be utilized. In this paper, an improved PSDM method using phase shift full bridge (PSFB) converter is proposed. This method introduces a phase control based freedom in the conventional PSFB control loop to realize communication using the same power conversion circuit. In this way, decoupled data modulation and power conversion are realized without extra wiring and coupling units, and thus the system structure is simplified. More importantly, the signal intensity can be regulated by the proposed perturbation depth, and so this method can adapt to different operating conditions. Application of the proposed method to a DC distributed power system composed of several PSFB converters is discussed. A 2kW prototype system with an embedded 5kbps communication link has been implemented, and the effectiveness of the method is verified by experimental results.

Application of automatic load transfer on feeder utilisation balance

This paper looks at how automatic load transfer may be used as a possible planning tool to help deliver faster connections for customers. A trial on an area of overhead line Network is presented to show how improvements in % feeder utilisation may be realised by changing the location of the open point. The reported Network data is compared to calculated data under two different configurations over a two week trial period. The results show that ALT open point determination in the presence of generation is different from a load only circuit and that the open points may not be fixed with time. Looking at improvements in Network headroom may not be conducive to other improvements in the network such as loss reduction or improving voltage profiles.

Cooperative and adaptive medium access control in multi-hop wireless networks

Due to low cost and easy deployment, multi-hop wireless networks become a very attractive communication paradigm. However, IEEE 802.11 medium access control (MAC) protocol widely used in wireless LANs was not designed for multi-hop wireless networks. Although it can support some kinds of ad hoc network architecture, it does not function efficiently in those wireless networks with multi-hop connectivity. Therefore, our research is focused on studying the medium access control in multi-hop wireless networks. The objective is to design practical MAC layer protocols for supporting multihop wireless networks. Particularly, we try to prolong the network lifetime without degrading performances with small battery-powered devices and improve the system throughput with poor quality channels. ^ In this dissertation, we design two MAC protocols. The first one is aimed at minimizing energy-consumption without deteriorating communication activities, which provides energy efficiency, latency guarantee, adaptability and scalability in one type of multi-hop wireless networks (i.e. wireless sensor network). Methodologically, inspired by the phase transition phenomena in distributed networks, we define the wake-up probability, which maintained by each node. By using this probability, we can control the number of wireless connectivity within a local area. More specifically, we can adaptively adjust the wake-up probability based on the local network conditions to reduce energy consumption without increasing transmission latency. The second one is a cooperative MAC layer protocol for multi-hop wireless networks, which leverages multi-rate capability by cooperative transmission among multiple neighboring nodes. Moreover, for bidirectional traffic, the network throughput can be further increased by using the network coding technique. It is a very helpful complement for current rate-adaptive MAC protocols under the poor channel conditions of direct link. Finally, we give an analytical model to analyze impacts of cooperative node on the system throughput. ^

Metodologia de estimação de estados para tratamento de medições errôneas e registros não sincronizados em algoritmos de localização de faltas em linhas de transmissão

The performance of algorithms for fault location i n transmission lines is directly related to the accuracy of its input data. Thus, fa ctors such as errors in the line parameters, failures in synchronization of oscillographic recor ds and errors in measurements of voltage and current can significantly influence the accurac y of algorithms that use bad data to indicate the fault location. This work presents a new method ology for fault location in transmission lines based on the theory of state estimation in or der to determine the location of faults more accurately by considering realistic systematic erro rs that may be present in measurements of voltage and current. The methodology was implemente d in two stages: pre-fault and post- fault. In the first step, assuming non-synchronized data, the synchronization angle and positive sequence line parameters are estimated, an d in the second, the fault distance is estimated. Besides calculating the most likely faul t distance obtained from measurement errors, the variance associated with the distance f ound is also determined, using the errors theory. This is one of the main contributions of th is work, since, with the proposed algorithm, it is possible to determine a most likely zone of f ault incidence, with approximately 95,45% of confidence. Tests for evaluation and validation of the proposed algorithm were realized from actual records of faults and from simulations of fictitious transmission systems using ATP software. The obtained results are relevant to show that the proposed estimation approach works even adopting realistic variances, c ompatible with real equipments errors.

Determinação da condutividade do solo para agricultura: uma metodologia utilizando propagação de ondas AM

Agriculture is an essential activity to the human development, the tendency is that their need to increase according to the increase in world population. It is very important to take the maximum performance that is possible of each land without degrading it, a frequently monitoring is essential for the best performance. The purpose of this work is, nondestructively, to monitor the surface electrical conductivity of the soil in a demarcated area, as on a plantation, using low frequency radio waves. The conductivity is directly linked to the amount of water in the area and nutrients, therefore a periodic or even permanent monitoring increases significantly the efficient of the use of the soil. They will be used long-wave radio transmission or medium whose main characteristic to spread over the surface of the earth. It is possible to choose an AM radio with location, frequency and power of the transmission known or generate the signal. The studied method computes the conductivity of the ground in a straight line between two measured points, so it can be used in smaller or larger size fields. Measurements were carried out using an electromagnetic field strength analyzer. The data obtained in the measurements are processed by a numerical calculation program, in our case Matlab. It is concluded that the recommendations of the ITU (International Telecommunication Union) on the conductivity of soil in Brazil is far from reality, on some routes the recommendations indicate the use of the electrical conductivity of the soil 1 mS/m, while the measurements was found 19 mS/m. With the method described a precision farmer, once initial research for about a year, can monitor the humidity and salinity of the land, with the ability to predict the area and the most suitable time for irrigation and fertilization, making management more efficient and less expensive, while optimizing water use, natural resource increasingly precious.

Teleconsultoria Off-line: uma arquitetura distribuída e compartilhada para sistemas heterogêneos

The Telehealth Brazil Networks Program, created in 2007 with the aim of strengthening primary care and the unified health system (SUS - Sistema Único de Saúde), uses information and communication technologies for distance learning activities related to health. The use of technology enables the interaction between health professionals and / or their patients, furthering the ability of Family Health Teams (FHT). The program is grounded in law, which determines a number of technologies, protocols and processes which guide the work of Telehealth nucleus in the provision of services to the population. Among these services is teleconsulting, which is registered consultation and held between workers, professionals and managers of healthcare through bidirectional telecommunication instruments, in order to answer questions about clinical procedures, health actions and questions on the dossier of work. With the expansion of the program in 2011, was possible to detect problems and challenges that cover virtually all nucleus at different scales for each region. Among these problems can list the heterogeneity of platforms, especially teleconsulting, and low internet coverage in the municipalities, mainly in the interior cities of Brazil. From this perspective, the aim of this paper is to propose a distributed architecture, using mobile computing to enable the sending of teleconsultation. This architecture works offline, so that when internet connection data will be synchronized with the server. This data will travel on compressed to reduce the need for high transmission rates. Any Telehealth Nucleus can use this architecture, through an external service, which will be coupled through a communication interface.

First science with SHARDS: emission line galaxies

SHARDS is an unbiased ultra-deep spectro-photometric survey with GTC@OSIRIS aimed at selecting and studying massive passively evolving galaxies at z=1.0-2.3 using a set of 24 medium-band filters (FWHM~17 nm) at 500-950 nm in GOODS-N. Our observing strategy is optimized to detect at z>1 the prominent Mg absorption feature at rest-frame ~ 280 nm, a distinctive, necessary, and sufficient feature of evolved stellar populations. Nonetheless, the data quality allow a plethora of studies on galaxy populations, including Emission Lines Galaxies (ELGs) about which we have started our first science verification project presented in this contribution.

Effects of alternating current voltage amplitude and oxide capacitance on mid-gap interface state defect density extractions in In0.53Ga 0.47As capacitors

This work looks at the effect on mid-gap interface state defect density estimates for In0.53Ga0.47As semiconductor capacitors when different AC voltage amplitudes are selected for a fixed voltage bias step size (100 mV) during room temperature only electrical characterization. Results are presented for Au/Ni/Al2O3/In0.53Ga0.47As/InP metal–oxide–semiconductor capacitors with (1) n-type and p-type semiconductors, (2) different Al2O3 thicknesses, (3) different In0.53Ga0.47As surface passivation concentrations of ammonium sulphide, and (4) different transfer times to the atomic layer deposition chamber after passivation treatment on the semiconductor surface—thereby demonstrating a cross-section of device characteristics. The authors set out to determine the importance of the AC voltage amplitude selection on the interface state defect density extractions and whether this selection has a combined effect with the oxide capacitance. These capacitors are prototypical of the type of gate oxide material stacks that could form equivalent metal–oxide–semiconductor field-effect transistors beyond the 32 nm technology node. The authors do not attempt to achieve the best scaled equivalent oxide thickness in this work, as our focus is on accurately extracting device properties that will allow the investigation and reduction of interface state defect densities at the high-k/III–V semiconductor interface. The operating voltage for future devices will be reduced, potentially leading to an associated reduction in the AC voltage amplitude, which will force a decrease in the signal-to-noise ratio of electrical responses and could therefore result in less accurate impedance measurements. A concern thus arises regarding the accuracy of the electrical property extractions using such impedance measurements for future devices, particularly in relation to the mid-gap interface state defect density estimated from the conductance method and from the combined high–low frequency capacitance–voltage method. The authors apply a fixed voltage step of 100 mV for all voltage sweep measurements at each AC frequency. Each of these measurements is repeated 15 times for the equidistant AC voltage amplitudes between 10 mV and 150 mV. This provides the desired AC voltage amplitude to step size ratios from 1:10 to 3:2. Our results indicate that, although the selection of the oxide capacitance is important both to the success and accuracy of the extraction method, the mid-gap interface state defect density extractions are not overly sensitive to the AC voltage amplitude employed regardless of what oxide capacitance is used in the extractions, particularly in the range from 50% below the voltage sweep step size to 50% above it. Therefore, the use of larger AC voltage amplitudes in this range to achieve a better signal-to-noise ratio during impedance measurements for future low operating voltage devices will not distort the extracted interface state defect density.

Extended urbanization in small and medium-sized cities: the case of Cirebon Indonesia

Although urbanization in smaller cities is arguably not imperative, the future of urban living is no longer expected to be principally in mega-cities. People increasingly live in intermediate and smaller cities, in line with the proportion of people residing in urban areas, which is also gradually rising. Smaller cities in Indonesia, like other smaller cities in the developing world, are relatively densely populated, and many of them are experiencing extended urbanization, thereby exceeding their administrative boundaries. This paper seeks to explore the factors triggering urban development in these smaller cities, for a case in Indonesia. Urban change in Cirebon Region has accelerated in recent years, very much in line with the decentralization policy in Indonesia. This paper shows how urban change is in!uenced by economic restructuring, which encourages people to live closer to the core of the region, representing a new link between the core and new emerging urban areas in the region. This paper reveals these attributes to identify the characteristics of smaller urban centres, thereby contributing a more nuanced image of small cities in general.