Design,optimization and in vivo evaluation of non-viral vectors for gene therapy. Applications in ocular disorders

227 págs.

A study of populations of the anchoveta, Cetengraulis mysticetus, based on meristic characters

ENGLISH: This study was undertaken to determine whether meristic characters indicate that more than one major population of anchovetas occurs in the range of the species from Mexico to Peru. Interest in this species lies in the fact that it is the principal bait fish used to catch yellowfin and skipjack tunas in the Eastern Pacific. Specimens examined were from collections made by California tuna fishing vessels at six major baiting localities covering nearly the entire range of the species, namely, Almejas Bay on the outer coast of Baja California, Guaymas and Ahome Point in the Gulf of California, Gulf of Fonseca, Gulf of Panama, and Gulf of Guayaquil. Four meristic characters were selected for study: vertebrae, dorsal fin rays, anal fin rays, and gill rakers on the first gill arch. Vertebral counts, using X-ray film, were taken from a total of 1,500 fish, 250 each from each of the six localities. For the other characters, 125 anchovetas were examined from each locality for a total of 750, the counts being made with the aid of a binocular microscope. Specimens were between 80 and 165 mm. standard length. SPANISH: Este estudio ha sido hecho con el propósito de determinar si los caracteres numéricos de las anchovetas indican que existe más de una población de este pez en la zona en que se encuentra la especie, comprendida entre México y Perú. El interés en dicha especie radica en el hecho de que éste es el pez de carnada usado principalmente para la pesca de los atunes "aleta amarilla" y "barrilete" en el Pacífico Oriental. Los especímenes que han sido examinados, se tomaron de las muestras recogidas por los barcos atuneros de California en seis de las mejores localidades en que se pesca la anchoveta, las cuales comprenden casi toda la zona en donde se encuentra la especie, a saber, Bahía de Almejas en la costa exterior de Baja California, Guaymas y Punta Ahorne en el Golfo de California, el Golfo de Fonseca, el Golfo de Panamá y el Golfo de Guayaquil. Cuatro caracteres numéricos fueron escogidos para su estudio: los que presentan 1) las vértebras, 2) los radios de la aleta dorsal, 3) los radios de la aleta anal y 4) las branquispinas del primer arco branquial. Mediante el uso de películas con rayos X, se contaron las vértebras en un total de 1,500 peces, es decir, 250 de cada una de las seis mencionadas localidades. En relación con los otros caracteres, se examinaron 125 anchovetas de cada área, o sea, un total de 750 ejemplares, habiendo sido hecho el conteo por medio de un microscopio binocular. Los especímenes tenían un largo standard entre 80 y 165 milímetros. (PDF contains 24 pages.)

A Featureless Approach to 3D Polyhedral Building Modeling from Aerial Images

This paper presents a model-based approach for reconstructing 3D polyhedral building models from aerial images. The proposed approach exploits some geometric and photometric properties resulting from the perspective projection of planar structures. Data are provided by calibrated aerial images. The novelty of the approach lies in its featurelessness and in its use of direct optimization based on image rawbrightness. The proposed framework avoids feature extraction and matching. The 3D polyhedral model is directly estimated by optimizing an objective function that combines an image-based dissimilarity measure and a gradient score over several aerial images. The optimization process is carried out by the Differential Evolution algorithm. The proposed approach is intended to provide more accurate 3D reconstruction than feature-based approaches. Fast 3D model rectification and updating can take advantage of the proposed method. Several results and evaluations of performance from real and synthetic images show the feasibility and robustness of the proposed approach.

Primeira parte das cartas familiares de D. Francisco Manuel escritas a varias pessoas sobre assuntos diversos /recolhidas, e publicadas em Cinco Centurias por Antonio Luis de Azevedo, professor de Humanidades e por elle offerecidas a Illustriss. Doutiss. e Sempre Insigne Academia dos Generosos (sic.)

Francisco Manuel de Melo nasceu em Lisboa, em 23 de novembro de 1608, e morreu na sua quinta em Alcântara, em 13 de outubro de 1966. Estudou no colégio jesuíta, especializando-se em Filosofia e Teologia. Aos dezessete anos, decidiu seguir carreira militar. Condenado por instigar homicídio, esteve preso por onze anos, e, em 1655, foi degredado para o Brasil, para onde partiu na armada de Francisco de Brito Freire. Retornou a Portugal em 1658. De lá foi para a Itália, onde permaneceu por alguns anos e começou, em 1664, a fazer uma edição completa de suas obras, às quais, por motivo ignorado, não deu continuidade. Transferiu-se para Lisboa, onde, logo depois, veio a falecer. Historiador, poeta, orador e crítico-moralista, Francisco Manuel foi um dos escritores mais eruditos e polidos.

Neues aus der Selektionsforschung am Ostseedorsch

In account of the decision of the IBSFC (International Baltic Sea Fisheries Commission) to admit alternative codend constructions to the presently legal of diamond meshes with 120 mm mesh opening, since seven years a vivid research activity is encouraged at the fishing technology institutions around the Baltic. Though a center of gravity in this field of research lies in the EUproject BACOMA (participants Finland Sweden, Denmark) also the corresponding national institutes of other Baltic countries (Germany, Poland, Russia) have been active with similar investigations. German experiments in 1999 dealt with the effect of the use of netting made of double instead of single yarn on the selectivity. Further research was done on modifications of codends made from netting turned 90° from its original orientation and on simplified versions of the so-called Multipanel codend which consists of three equal diamond and three square mesh panels. The experiments demonstrated on the one hand a definite deterioration of the selective properties in a codend made from double instead of single yarn. Constructional changes in codends made from netting turned 90° on the other hand brought a further improvement of the selectivity of such codends. Finally, the investigations on the Multipanel codend revealed the overwhelming influence of the square mesh panels on the selectivity success. If in a conventional diamond codend three windows are cut out starting 50 cm from the real end of the codend in such a way that the remaining parts have the same breadth as the square mesh panels to insert, the selective effect of such a codend is remarkably improved. According to further preliminary tests this seems to be nearly independent from the type of material used.

A simple method to simulate shrinkage-induced cracking in cement-based composites by lattice-type modeling

A new numerical procedure is proposed to investigate cracking behaviors induced by mismatch between the matrix phase and aggregates due to matrix shrinkage in cement-based composites. This kind of failure processes is simplified in this investigation as a purely spontaneous mechanical problem, therefore, one main difficulty during simulating the phenomenon lies that no explicit external load serves as the drive to propel development of this physical process. As a result, it is different from classical mechanical problems and seems hard to be solved by using directly the classical finite element method (FEM), a typical kind of "load -> medium -> response" procedures. As a solution, the actual mismatch deformation field is decomposed into two virtual fields, both of which can be obtained by the classical FEM. Then the actual response is obtained by adding together the two virtual displacement fields based on the principle of superposition. Then, critical elements are detected successively by the event-by-event technique. The micro-structure of composites is implemented by employing the generalized beam (GB) lattice model. Numerical examples are given to show the effectiveness of the method, and detailed discussions are conducted on influences of material properties.

Potentials for fisheries in the Niger Delta: Another green light for self sufficiency in regional food production

A discussion is presented on the potential for fishery development in the Niger Delta region, considering engineering activities and food production potentials of the freshwater zone and immediate hinterland, the brackishwater mangrove swamps and the estuaries. An examination of current trends in the environment indicates that a possible solution to improved exploitation of the region lies in hydraulic engineering, the manipulation of environmental conditions through varying freshwater and seawater inputs so as to increase aquatic and wetland productivity

The past history and present trends in the fisheries of Lake Chad

Lake Chad is a very large, shallow eutrophic lake shared by Chad, Nigeria, Niger and Cameroun. It supplies approximately 13% of Nigeria's inland fish. It however lies in an unstable ecological environment characterised by intermittent period of rainfall and drought. This creates a very large draw down area. Consequently, the fisheries are affected by the oscillation in the size of lake due to the drought. Other factors affecting the volume of water are the numerous dams on the inflow rivers. The fishery is also subjected to intense overfishing and may be affected by pollution and other land use practices. The paper discusses changes that took place over the years as a result of the factors of drought, effect of dams on the inflow rivers. The fishery is also subjected to intense overfishing and may be affected by pollution and other land use practices. The paper discusses changes that took place over the years as a result of the factors of drought, effects of dams and overexploitation. Previous records of fish production, species composition and distribution, the status of the fish stocks, their sizes are compared with more recent data. The status of the fishery before and after the contraction of the lake is discussed. Suggestion for a national exploitation of the lake based on habitat improvement, increasing the volume of the water in the lake through controlled use of the influent rivers as well as reduction in overfishing are made

Validation of daily growth increments and estimation of growth rates of larval and early juvenile black skipjack, Euthynnus lineatus, using otoliths

ENGLISH: Increments in otoliths (sagittae) were examined, using light and scanning electron microscopy, to determine ages and estimate growth rates of larval and early-juvenile black skipjack, Euthynnus lineatus. Larvae and juveniles were collected between 1987 and 1989 from coastal waters of Panama in the eastern Pacific Ocean. Results from a laboratory experiment indicated that immersion for 6 and 12 hours in a 200 mg/L solution of tetracycline hydrochloride adequately marks otoliths and that increments are formed daily in the sagittae of postflexion larvae and early juveniles. Further, survival rates of tetracycline-treated fish were not significantly different from those of control fish. Growth rates were derived from length-age relationships of 218 field-collected specimens ranging in size from 5.7 to 20.3 mm SL. A growth rate of 0.70 mm/d was estimated from the weighted regression of standard length on age for all specimens. This rate lies within the range reported for larvae and early juveniles of other species of subtropical and tropical scombrids. Growth rates of postflexion larvae and early juveniles were not significantly different between the rainy season in July-August 1988 and the dry, upwelling season in January-February 1989. Growth was, however, significantly more variable for older individuals in July-August than in January-February, and may correspond, in part, to seasonal patchiness of prey. The growth rates of the otoliths relative to fish length were also not significantly different between seasons; however, the otoliths were larger relative to the lengths of fish collected in the rainy season, which may reflect slower growth during earlier larval stages. SPANISH: Se examinaron incrementos en otolitos (ságitas), usando microscopia de luz y de barrido electrónico, a fin de determinar la edad y estimar las tasas de crecimiento de barriletes negros, Euthynnus lineatus, larvales y juveniles tempranos. Entre 1987 y 1989 se capturaron larvas y juveniles en las aguas costeras de Panamá en el Océano Pacífico oriental. Los resultados de un experimento de laboratorio indicaron que una inmersión de 6 a 12 horas de duración en una solución de 200 mg/L de hidrocloro de tetraciclina marca los otolitos adecuadamente y que los incrementos se forman a diario en las ságitas de larvas en postflexión y juveniles tempranos. Además, las tasas de supervivencia de los peces tratados con tetraciclina no fueron significativamente diferentes a aquellas de los peces de control. Se calcularon las tasas de crecimiento a partir de las relaciones de talla-edad de 218 especímenes de TE entre 5.7 y 20.3 mm capturados en el mar. Se estimó.una tasa de crecimiento de 0.70 mm/día a partir de la regresión ponderada de talla estándar sobre edad para todos los especímenes. Esta tasa cae dentro del rango reportado para larvas y juveniles tempranos de otras especies de escómbridos subtropicales y tropicales. Las tasas de crecimiento de larvas en postflexión y juveniles tempranos no fueron significativamente diferentes entre la temporada de lluvias en julio-agosto de 1988 y la temporada de sequía y afloramiento en enero-febrero de 1989. Sin emoargo, el crecimiento fue significativamente más variable para los individuos de mayor edad en julio-agosto que en enero-febrero, y quizás corresponda parcialmente a la irregularidad temporal de la abundancia de presas. Las tasas de crecimiento de los otolitos en relación a la talla de los peces tampoco fueron significativamente diferentes entre temporadas; sin embargo, los otolitos eran más grandes en relación a la talla en peces capturados en la temporada de lluvias, lo cual podría reflejar crecimiento más lento durante las etapas larvales más tempranas. (PDF contains 42 pages.)

Documentación espacial del patrimonio: preservación de la información. Necesidades, posibilidades, estrategias y estándares

[ES] La parte práctica de esta tesis se centra en un proyecto ERASMUS realizado en el Monasterio de San Prudencio de Monte Laturce (Clavijo, La Rioja). Dicho proyecto aparece descrito en varios registros de este mismo repositorio a los que se puede acceder a través del siguiente:

Photographic investigations in molecular spectroscopy

The determination of the energy levels and the probabilities of transition between them, by the formal analysis of observed electronic, vibrational, and rotational band structures, forms the direct goal of all investigations of molecular spectra, but the significance of such data lies in the possibility of relating them theoretically to more concrete properties of molecules and the radiation field. From the well developed electronic spectra of diatomic molecules, it has been possible, with the aid of the non-relativistic quantum mechanics, to obtain accurate moments of inertia, molecular potential functions, electronic structures, and detailed information concerning the coupling of spin and orbital angular monenta with the angular momentum of nuclear rotation. The silicon fluori1e molecule has been investigated in this laboratory, and is found to emit bands whose vibrational and rotational structures can be analyzed in this detailed fashion.

Like silicon fluoride, however, the great majority of diatomic molecules are formed only under the unusual conditions of electrical discharge, or in high temperature furnaces, so that although their spectra are of great theoretical interest, the chemist is eager to proceed to a study of polyatomic molecules, in the hope that their more practically interesting structures might also be determined with the accuracy and assurance which characterize the spectroscopic determinations of the constants of diatomic molecules. Some progress has been made in the determination of molecule potential functions from the vibrational term values deduced from Raman and infrared spectra, but in no case can the calculations be carried out with great generality, since the number of known term values is always small compared with the total number of potential constants in even so restricted a potential function as the simple quadratic type. For the determination of nuclear configurations and bond distances, however, a knowledge of the rotational terms is required. The spectra of about twelve of the simpler polyatomic molecules have been subjected to rotational analyses, and a number of bond distances are known with considerable accuracy, yet the number of molecules whose rotational fine structure has been resolved even with the most powerful instruments is small. Consequently, it was felt desirable to investigate the spectra of a number of other promising polyatomic molecules, with the purpose of carrying out complete rotational analyses of all resolvable bands, and ascertaining the value of the unresolved band envelopes in determining the structures of such molecules, in the cases in which resolution is no longer possible. Although many of the compounds investigated absorbed too feebly to be photographed under high dispersion with the present infrared sensitizations, the location and relative intensities of their bands, determined by low dispersion measurements, will be reported in the hope that these compounds may be reinvestigated in the future with improved techniques.

Mejorar la competividad mediante la colaboración interempresarial en el sector auxiliar de automoción

[ES] En este trabajo se pone de manifiesto la importancia de la colaboración a través de los clusters, en uno de los sectores industriales de mayor importancia en España: el sector de “Fabricantes de Equipos y Componentes para la Automoción”. Esto es así debido a que el sector de Fabricación de Automóviles cada vez delega más funciones de I+D+i y de coordinación con proveedores a las empresas auxiliares de automoción. Sin embargo, el hecho de ser éste un sector compuesto mayoritariamente por PYMEs dificulta la óptima realización de las mismas en solitario, por lo que la pertenencia a organizaciones que reúnan en su seno a los agentes de la cadena de valor, competidores e instituciones resulta decisiva a la hora de mantenerse en el mercado. Para confirmar el planteamiento teórico establecido, se realiza un análisis cualitativo comparativo entre el Cluster de Automoción del País Vasco y el resto de las empresas del Sector. Por último, con el fin de aportar herramientas, que permitan mejorar la competitividad, en un futuro marcado por la globalización y la crisis analizamos el Cluster de Fabricantes de Equipos y Componentes para la Automoción del País Vasco.

Applying Habermas's critical theory to public administration and policy: A case study of Florida Everglades restoration program

The foundation of Habermas's argument, a leading critical theorist, lies in the unequal distribution of wealth across society. He states that in an advanced capitalist society, the possibility of a crisis has shifted from the economic and political spheres to the legitimation system. Legitimation crises increase the more government intervenes into the economy (market) and the "simultaneous political enfranchisement of almost the entire adult population" (Holub, 1991, p. 88). The reason for this increase is because policymakers in advanced capitalist democracies are caught between conflicting imperatives: they are expected to serve the interests of their nation as a whole, but they must prop up an economic system that benefits the wealthy at the expense of most workers and the environment. Habermas argues that the driving force in history is an expectation, built into the nature of language, that norms, laws, and institutions will serve the interests of the entire population and not just those of a special group. In his view, policy makers in capitalist societies are having to fend off this expectation by simultaneously correcting some of the inequities of the market, denying that they have control over people's economic circumstances, and defending the market as an equitable allocator of income. (deHaven-Smith, 1988, p. 14). Critical theory suggests that this contradiction will be reflected in Everglades policy by communicative narratives that suppress and conceal tensions between environmental and economic priorities. Habermas’ Legitimation Crisis states that political actors use various symbols, ideologies, narratives, and language to engage the public and avoid a legitimation crisis. These influences not only manipulate the general population into desiring what has been manufactured for them, but also leave them feeling unfulfilled and alienated. Also known as false reconciliation, the public's view of society as rational, and "conductive to human freedom and happiness" is altered to become deeply irrational and an obstacle to the desired freedom and happiness (Finlayson, 2005, p. 5). These obstacles and irrationalities give rise to potential crises in the society. Government's increasing involvement in Everglades under advanced capitalism leads to Habermas's four crises: economic/environmental, rationality, legitimation, and motivation. These crises are occurring simultaneously, work in conjunction with each other, and arise when a principle of organization is challenged by increased production needs (deHaven-Smith, 1988). Habermas states that governments use narratives in an attempt to rationalize, legitimize, obscure, and conceal its actions under advanced capitalism. Although there have been many narratives told throughout the history of the Everglades (such as the Everglades was a wilderness that was valued as a wasteland in its natural state), the most recent narrative, “Everglades Restoration”, is the focus of this paper.(PDF contains 4 pages)

Multiwavelength observations of the black hole candidate 1E 1740.7-2942

Observations of the Galactic center region black hole candidate 1E 1740.7-2942 have been carried out using the Caltech Gamma-Ray Imaging Payload (GRIP), the Röntgensatellit (ROSAT) and the Very Large Array (VLA). These multiwavelength observations have helped to establish the association between a bright emitter of hard X-rays and soft γ-rays, the compact core of a double radio jet source, and the X-ray source, 1E 1740.7-2942. They have also provided information on the X-ray and hard X-ray spectrum.

The Galactic center region was observed by GRIP during balloon flights from Alice Springs, NT, Australia on 1988 April 12 and 1989 April 3. These observations revealed that 1E 1740.7-2942 was the strongest source of hard X-rays within ~10° of the Galactic center. The source spectrum from each flight is well fit by a single power law in the energy range 35-200 keV. The best-fit photon indices and 100 keV normalizations are: γ = (2.05 ± 0.15) and K_(100) = (8.5 ± 0.5) x 10^(-5) cm^(-2) s^(-1) keV^(-1) and γ = (2.2 ± 0.3) and K_(100) = (7.0 ± 0.7) x 10^(-5) cm^(-2) s^(-1) keV^(-1) for the 1988 and 1989 observations respectively. No flux above 200 keV was detected during either observation. These values are consistent with a constant spectrum and indicate that 1E 1740.7-2942 was in its normal hard X-ray emission state. A search on one hour time scales showed no evidence for variability.

The ROSAT HRI observed 1E 1740.7-2942 during the period 1991 March 20-24. An improved source location has been derived from this observation. The best fit coordinates (J2000) are: Right Ascension = 17^h43^m54^s.9, Declination = -29°44'45".3, with a 90% confidence error circle of radius 8".5. The PSPC observation was split between periods from 1992 September 28- October 4 and 1993 March 23-28. A thermal bremsstrahlung model fit to the data yields a column density of N_H = 1.12^(+1.51)_(0.18) x cm^(-2) , consistent with earlier X- ray measurements.

We observed the region of the Einstein IPC error circle for 1E 1740.7-2942 with the VLA at 1.5 and 4.9 GHz on 1989 March 2. The 4.9 GHz observation revealed two sources. Source 'A', which is the core of a double aligned radio jet source (Mirabel et al. 1992), lies within our ROSAT error circle, further strengthening its identification with 1E 1740.7-2942.

Synthesis, structure, and photophysics of polypyridophenazine transition-metal complexes

The condensation of phenanthroline-5,6-dione (phendione) with polyamines is a versatile synthetic route to a wide variety of chelating ligands. Condensation with 2,3- napthalene diamine gives benzo[i]dipyrido[3,2-a:2',3'-c]phenazine (bdppz) a ligand containing weakly-coupled orbitals of benzophenazine (bpz) and 2,2' -bipyridinde(bpy) character. The bpy character gives Re and Ru complexes excited-state redox properties; intramolecular electron transfer (ET) takes place to the bpz portion of the ligand. The charge-separated state so produced has an extraordinarily-long 50 µs lifetime. The slow rate of charge recombination arises from a combination of extremely weak coupling between the metal center and the bpz acceptor orbital and Marcus "inverted region" behavior. Molecular orbital calculations show that only 3% the electron density in the lowest unoccupied molecular orbital lies on the bpy atoms of bdppz, effectively trapping the transferred electron on the bpz portion. The rate of charge recombination decreases with increasing driving force, showing that these rates lie in the inverted region. Comparison of forward and back ET rates shows that donor-acceptor coupling is four orders of magnitude greater for photoinduced electron transfer than it is for thermal charge recombination.

Condensation of phendione with itself or tetramines gives a series of binucleating tetrapyridophenazine ligands of incrementally-varying coordination-site separation. When a photoredox-active metal center is attached, excited-state energy and electron transfer to an acceptor metal center at the other coordination site can be studied as a function of distance. A variety of monometallic and homo- and heterodimetallic tetrapyridophenazine complexes has been synthesized. Electro- and magnetochemistry show that no ground-state interaction exists between the metals in bimetallic complexes. Excited-state energy and electron transfer, however, takes place at rates which are invariant with increasing donor-acceptor separation, indicating that a very efficient coupling mechanism is at work. Theory and experiment have suggested that such behavior might exist in extended π-systems like those presented by these ligands.

Condensation of three equivalents of 4,5-dimethyl-1,2-phenylenediamine with hexaketocyclohexane gives the trinucleating ligand hexaazahexamethyltrinapthalene (hhtn). Attaching two photredox-active metal centers and a third catalytic center to hhtn provides means by which multielectron photocatalyzed reactions might be carried out. The coordination properties of hhtn have been examined; X-ray crystallographic structure determination shows that the ligand's constricted coordination pocket leads to distorted geometries in its mono- and dimetallic derivatives.