Deployment of an all-in-one millimetre-wave anechoic chamber

This paper describes the new anechoic chamber available at The University of Kent, UK. This facility includes a spherical near/far field, planar near field, cylindrical near field and a compact range. The facility allows measurement from 600 MHz up to 110 MHz. The spherical, planar and cylindrical ranges covers up to 40 GHz and the compact range is available from 50 GHz up to 110 MHz. Immediate plans are to use the new facility to measure body-centric antennas and sensing nodes together with near field sampling of finite sized Frequency Selective Surfaces.

Vivienda colectiva en Carabanchel

análisis de la vivienda colectiva en España a través del caso de estudio del edificio de viviendas de Carabanchel de Amann - Canovas - Maruri

Visualizador de programas Java concurrentes

El presente trabajo fin de grado, que, a partir de ahora, denominaré TFG, consiste en elaborar una monitorización de programas concurrentes en lenguaje Java, para que se visualicen los eventos ocurridos durante la ejecución de los dichos programas. Este trabajo surge en el marco de la asignatura “Concurrencia” de la Escuela Técnica Superior de Ingeniería Informática de la Universidad Politécnica de Madrid, impartida por D. Julio Mariño y D. Ángel Herranz. El objetivo principal de este proyecto es crear una herramienta para el aprendizaje de la asignatura de concurrencia, facilitando la comprensión de los conceptos teóricos, de modo que puedan corregir los posibles errores que haya en sus prácticas. en este proyecto se expone el desarrollo de una librería de visualización de programas concurrentes programados en Java usando un formalismo gráfico similar al empleado en la asignatura. Además esta librería da soporte a los mecanismos de sincronización usados en las prácticas de la asignatura: la librería Monitor (desarrollada por los profesores de la asignatura, D. Ángel Herranz y D. Julio Mariño) y la librería JCSP (Universidad de Kent). ---ABSTRACT---This Bachelor Thesis addresses the problem of monitoring a Java program in order to trace and visualize a certain set of events produced during the execution of concurrent Java programs. This work originates in the subject "Concurrency" of the Computer Science and Engineering degree of our University. The main goal of this work is to have a tool that helps students learning the subject, so they can better understand the core concepts and correct common mistakes in the course practical work. We have implemented a library for visualizing concurrent Java programsusing a graphical notation similar to the one used in class, which supports the design of concurrent programs whose synchronization mechanisms are either monitors(using the Monitor package) or CSP(as implemented in the JCSP library from Kent University).

Sequence-specific polypeptoids: A diverse family of heteropolymers with stable secondary structure

We have synthesized and characterized a family of structured oligo-N-substituted-glycines (peptoids) up to 36 residues in length by using an efficient solid-phase protocol to incorporate chemically diverse side chains in a sequence-specific fashion. We investigated polypeptoids containing side chains with a chiral center adjacent to the main chain nitrogen. Some of these sequences have stable secondary structure, despite the achirality of the polymer backbone and its lack of hydrogen bond donors. In both aqueous and organic solvents, peptoid oligomers as short as five residues give rise to CD spectra that strongly resemble those of peptide α-helices. Differential scanning calorimetry and CD measurements show that polypeptoid secondary structure is highly stable and that unfolding is reversible and cooperative. Thermodynamic parameters obtained for unfolding are similar to those obtained for the α-helix to coil transitions of peptides. This class of biomimetic polymers may enable the design of self-assembling macromolecules with novel structures and functions.

NMR determination of the major solution conformation of a peptoid pentamer with chiral side chains

Polymers of N-substituted glycines (“peptoids”) containing chiral centers at the α position of their side chains can form stable structures in solution. We studied a prototypical peptoid, consisting of five para-substituted (S)-N-(1-phenylethyl)glycine residues, by NMR spectroscopy. Multiple configurational isomers were observed, but because of extensive signal overlap, only the major isomer containing all cis-amide bonds was examined in detail. The NMR data for this molecule, in conjunction with previous CD spectroscopic results, indicate that the major species in methanol is a right-handed helix with cis-amide bonds. The periodicity of the helix is three residues per turn, with a pitch of ≈6 Å. This conformation is similar to that anticipated by computational studies of a chiral peptoid octamer. The helical repeat orients the amide bond chromophores in a manner consistent with the intensity of the CD signal exhibited by this molecule. Many other chiral polypeptoids have similar CD spectra, suggesting that a whole family of peptoids containing chiral side chains is capable of adopting this secondary structure motif. Taken together, our experimental and theoretical studies of the structural properties of chiral peptoids lay the groundwork for the rational design of more complex polypeptoid molecules, with a variety of applications, ranging from nanostructures to nonviral gene delivery systems.

Activation changes the spectrum but not the diversity of genes expressed by T cells

During activation T cells are thought to change their patterns of gene expression dramatically. To find out whether this is true for T cells activated in animals, the patterns of genes expressed in resting T cells and T cells 8 and 48 hr after activation were examined by using Affymetrix gene arrays. Gene arrays gave accurate comparisons of gene expression in the different cell types because the expression of genes known to vary during activation changed as expected. Of the approximately 6,300 genes assessed by the arrays, about one-third were expressed to appreciable extents in any of the T cells tested. Thus, resting T cells express a surprisingly large diversity of genes. The patterns of gene expression changed considerably within 8 hr of T cell activation but returned to a disposition more like that of resting T cells within 48 hr of exposure to antigen. Not unexpectedly, the activated T cells expressed genes associated with cell division at higher levels than resting T cells. The resting T cells expressed a number of cytokine receptor genes and some genes thought to suppress cell division, suggesting that the state of resting T cells is not a passive failure to respond to extant external stimuli.

On the electronic nature of low-barrier hydrogen bonds in enzymatic reactions

The electronic nature of low-barrier hydrogen bonds (LBHBs) in enzymatic reactions is discussed based on combined low temperature neutron and x-ray diffraction experiments and on high level ab initio calculations by using the model substrate benzoylacetone. This molecule has a LBHB, as the intramolecular hydrogen bond is described by a double-well potential with a small barrier for hydrogen transfer. From an “atoms in molecules” analysis of the electron density, it is found that the hydrogen atom is stabilized by covalent bonds to both oxygens. Large atomic partial charges on the hydrogen-bonded atoms are found experimentally and theoretically. Therefore, the hydrogen bond gains stabilization from both covalency and from the normal electrostatic interactions found for long, weak hydrogen bonds. Based on comparisons with other systems having short-strong hydrogen bonds or LBHBs, it is proposed that all short-strong and LBHB systems possess similar electronic features of the hydrogen-bonded region, namely polar covalent bonds between the hydrogen atom and both heteroatoms in question.

Synthesis and screening of small molecule libraries active in binding to DNA

Five synthetic combinatorial libraries of 2,080 components each were screened as mixtures for inhibition of DNA binding to two transcription factors. Rapid, solution-phase synthesis coupled to a gel-shift assay led to the identification of two compounds active at a 5- to 10-μM concentration level. The likely mode of inhibition is intercalation between DNA base pairs. The efficient deconvolution through sublibrary synthesis augurs well for the use of large mixtures of small, nonpeptide molecules in biological screens.