Évolution des pratiques alimentaires en cours de vie : une étude isotopique multi-matérielle de la population du cimetière Saint-Matthew (Québec, 1771-1860)

En tant que population majoritairement immigrante, les protestants inhumés au cimetière Saint-Matthew, ville de Québec (1771-1860) ont dû s'adapter à un nouvel environnement à leur arrivée au Québec, et donc à de nouvelles ressources. Parallèlement, les 18e et 19e siècles sont marqués par un contexte socio-économique en pleine mutation avec l'arrivée graduelle de la période industrielle, et la ville de Québec, avec son contexte portuaire, a ainsi été au coeur de ces changements. L'objectif de ce mémoire est d'évaluer si la géochimie des isotopes stables appliquée à plusieurs matériaux du squelette humain (collagène et apatite de l'os, collagène de la dentine, et carbonate de l'émail) permet de mieux comprendre comment les comportements alimentaires des individus analysés provenant de ce cimetière cosmopolite (n=40) ont évolué en cours de vie. L'alimentation étant influencée par des conditions socio-économiques, culturelles et environnementales, cela peut nous informer indirectement sur les processus d'adaptation et l'identité d'un individu. C'est dans cette perspective d'écologie culturelle que nous avons interprété les données recueillies lors de ce projet, en complément aux analyses effectuées précédemment par Morland (2009) et Caron (2013). Nos résultats corroborent les tendances déjà observées, soit des pratiques alimentaires semblables à celles que l'on retrouve en Europe, et des immigrants provenant majoritairement des Îles Britanniques. Ils démontrent également une légère augmentation de la consommation de ressources C4, comme le maïs et la canne à sucre, à l'âge adulte pour 90% des individus analysés, de même qu'une baisse du niveau de protéines. Par ailleurs, les individus étudiés ont généralement eu tendance à conserver le même niveau alimentaire les uns par rapport aux autres tout au cours de leur vie, même si les pratiques étaient moins diversifiés à l'âge adulte. Finalement, on constate des similarités de comportements avec les populations irlandaises et britanniques plus pauvres durant l'enfance, alors qu'ils ressemblent davantage à ceux visibles dans la vallée laurentienne en fin de vie, notamment en ce qui concerne l'apport en protéines. Nos résultats suggèrent donc des changements alimentaires significatifs, fort possiblement liés aux processus de migration et à une adaptation constante à un nouvel environnement de la part des individus étudiés.

Complex permittivity and conductivity of poly aniline at microwave frequencies

Dielectric properties of polyaniline at different frequencies were studied. Cavity perturbation technique was employed for the study. Poly aniline in the powder and pelletised forms were prepared under different environmental conditions. Different samples of poly aniline exhibit high conductivity. However, the conductivity of samples prepared under different environmental conditions is found to vary. All the samples in the powder form have high conductivity irrespective of the method of preparation. The high conductivity at microwave frequency makes it possible to be used for developing microwave components like filters.

Marine yeasts — a review

Yeasts are ubiquitous in their distribution and populations mainly depend on the type and concentration of organic materials. The distribution of species, as well as their numbers and metabolic characteristics were found to be governed by existing environmental conditions. Marine yeasts were first discovered from the Atlantic Ocean and following this discovery, yeasts were isolated from different sources, viz. seawater, marine deposits, seaweeds, fish, marine mammals and sea birds. Nearshore environments are usually inhabited by tens to thousands of cells per litre of water, whereas low organic surface to deep-sea oceanic regions contain 10 or fewer cells/litre. Aerobic forms are found more in clean waters and fermentative forms in polluted waters. Yeasts are more abundant in silty muds than in sandy sediments. The isolation frequency of yeasts fell as the depth of the sampling site is increased. Major genera isolated in this study were Candida, Cryptococcus, Debaryomyces and Rhodotorula. For biomass estimation ergosterol method was used. Classification and identification of yeasts were performed using different criteria, i.e. morphology, sexual reproduction and physiological/biochemical characteristics. Fatty acid profiling or molecular sequencing of the IGS and ITS regions and 28S gene rDNA ensured accurate identification.

Regression models for bivariate survival data

Multivariate lifetime data arise in various forms including recurrent event data when individuals are followed to observe the sequence of occurrences of a certain type of event; correlated lifetime when an individual is followed for the occurrence of two or more types of events, or when distinct individuals have dependent event times. In most studies there are covariates such as treatments, group indicators, individual characteristics, or environmental conditions, whose relationship to lifetime is of interest. This leads to a consideration of regression models.The well known Cox proportional hazards model and its variations, using the marginal hazard functions employed for the analysis of multivariate survival data in literature are not sufficient to explain the complete dependence structure of pair of lifetimes on the covariate vector. Motivated by this, in Chapter 2, we introduced a bivariate proportional hazards model using vector hazard function of Johnson and Kotz (1975), in which the covariates under study have different effect on two components of the vector hazard function. The proposed model is useful in real life situations to study the dependence structure of pair of lifetimes on the covariate vector . The well known partial likelihood approach is used for the estimation of parameter vectors. We then introduced a bivariate proportional hazards model for gap times of recurrent events in Chapter 3. The model incorporates both marginal and joint dependence of the distribution of gap times on the covariate vector . In many fields of application, mean residual life function is considered superior concept than the hazard function. Motivated by this, in Chapter 4, we considered a new semi-parametric model, bivariate proportional mean residual life time model, to assess the relationship between mean residual life and covariates for gap time of recurrent events. The counting process approach is used for the inference procedures of the gap time of recurrent events. In many survival studies, the distribution of lifetime may depend on the distribution of censoring time. In Chapter 5, we introduced a proportional hazards model for duration times and developed inference procedures under dependent (informative) censoring. In Chapter 6, we introduced a bivariate proportional hazards model for competing risks data under right censoring. The asymptotic properties of the estimators of the parameters of different models developed in previous chapters, were studied. The proposed models were applied to various real life situations.

Economics of Ornamental Fisheries in Kerala

Comprehensive studies integrating production, consumption and marketing of ornamental fishes were never conducted in the past in Kerala and this study is an attempt to highlight the dynamics in a systematic manner by making a primary empirical study. The advantages of such an approach are many. First, the study entails detailed empirical data under a given time frame on various inter linked economic activities in ornamental fisheries sector. Second, the study tries to improve upon the previous discipline-bound studies by adopting an integrated approach. As Kerala has diverse ecological and climatic conditions, case studies on various ornamental fish production systems help identifying suitable culture practice for selected environmental conditions. One of the major conclusions of this study as explained in the concluding chapter is that although Kerala is blessed with favourable geographic and climatic conditions needed for developing ornamental fishery, diverse local conditions and requirements contain adopting modern methods of enterprise development.Consumer studies conducted would help to identify the demand of ornamental fishes and the factors leading to it. Market studies would help in understanding the forces behind domestic market, which is very promising, but neglected. Viability studies throw light on the economic performance of both the production systems and the trading units of ornamental fishes. Despite the economic significance of ornamental fisheries industry, its development has been constrained due to various bio-technical, cultural, socio-economic, organisational and above all, institutional and policy failures. The outcome of the study would identify constraints facing the industry and institutional arrangements for development.

Complex permittivity and conductivity of poly aniline at microwave frequencies

Dielectric properties of polyaniline at different frequencies were studied. Cavity perturbation technique was employed for the study. Poly aniline in the powder and pelletised forms were prepared under different environmental conditions. Different samples of poly aniline exhibit high conductivity. However. the conductivity of samples prepared under different environmental conditions is found to vary. All the samples in the powder form have high conductivity irrespective of the method of preparation. The high conductivity at microwave frequency makes it possible to be used for developing microwave components like filters.

Design and Development of re configurable compact cross patch antenna for switchable polarization

Antennas are necessary and vital components of communication and radar systems, but sometimes their inability to adjust to new operating scenarios can limit system performance. Reconfigurable antennas can adjust with changing system requirements or environmental conditions and provide additional levels of functionality that may result in wider instantaneous frequency bandwidths, more extensive scan volumes, and radiation patterns with more desirable side lobe distributions. Their agility and diversity created new horizons for different types of applications especially in cognitive radio, Multiple Input Multiple Output Systems, satellites and many other applications. Reconfigurable antennas satisfy the requirements for increased functionality, such as direction finding, beam steering, radar, control and command, within a confined volume. The intelligence associated with the reconfigurable antennas revolved around switching mechanisms utilized. In the present work, we have investigated frequency reconfigurable polarization diversity antennas using two methods: 1. By using low-loss, high-isolation switches such as PIN diode, the antenna can be structurally reconfigured to maintain the elements near their resonant dimensions for different frequency bands and/or polarization. 2. Secondly, the incorporation of variable capacitors or varactors, to overcome many problems faced in using switches and their biasing. The performances of these designs have been studied using standard simulation tools used in industry/academia and they have been experimentally verified. Antenna design guidelines are also deduced by accounting the resonances. One of the major contributions of the thesis lies in the analysis of the designed antennas using FDTD based numerical computation to validate their performance.

Resource abundance and Survival of indigenous ornamental fishes of central Kerala with emphasis on handling and packing stress in Puntius filamentosus(VALENCIENNES)

This thesis Entitled Resource abundance and survival of indigenous ornamental fishes of central kerala with emphasis on handling and packing stress in puntius filamentosus (valenciennes).Kerala state is endowed with 41 west flowing and three east flowing rivers originating in the Western Ghats. These rivers and their vast network of tributaries and distributaries harbour rich and diversified fish fauna. Most of the freshwater fishes available in Kerala are highly appreciated as ornamental fishes in the national and international markets.Today the ornamental fish industry is one of the largest industries all over the world. The demand for ornamental fishes has been increasing steadily with the enlargement of the industry, such that the current demand for indigenous ornamental fishes have exceeded the supply. This has led to serious concern about the resources available in the country that can be utilised judiciously for the economic benefit of the state. With an aim to fill up the lacuna, a database of freshwater ornamental fishes of Kerala was created as part of the present study. Ornamental fishes destined for export marketing should thrive well in the aquarium conditions.The study reiterates fishes caught from different environmental conditions and feeding habits have a greater ability to adapt and acclimatise to an entirely new environment and food habits. Marketing studies based on the statistics available with Marine Products Export Development Authority show that these species are not being exported at the required level over the past 6 years, when compared to the availability in the water bodies of Kerala. Sustainable utilisation of these resources from the wild using modern management principles and code of conduct for responsible fishing are advisable until captive breeding technology is popularised.

Studies on osmoregulation in the penaeid prawn Metapenaeus dobsoni (Miers)

Metapenaeus dobsoni (Miers) is the most abundant species along the coast of Kerala. It is cultured extensively by adoption of traditional farming practices. The geographical location and water source determines the seasonal and annual environmental fluctuations the prawn farming systems experiences. The life cycle of the shrimp includes its migration to the coastal deeper waters for spawning and the immigration of larvae to the estuaries for growth. The survival of the species in such complex ecosystems is thus critical to its life cycle. The animal adapts itself to different environments through a physiological process known as osmoregulation. The present study on osmoregulation in the penaeid prawn Metapenaeus dobsoni was thus undertaken to understand the mechanism adopted by this species to survive in different environments. A number of experimental work have been conducted to understand the effect of salinity on the internal variations. However the effect of the complex environmental conditions as existent in nature on the osmotic variations in this species has not been dealt with in any of the earlier studies.

Studies on the ecology of zooplankton of cochin backwaters(a tropical estuary)

Ecology is the study of systems at a level in which individuals or whole organisms may be considered elements of interaction, either among themselves, or with a loosely organised environmental matrix. Systems at this level are named ecosystems, and ecology, of course, is the biology of ecosystems" (Hargalef, 1968). This thesis includes principally, a study on the ecology of zooplankton of the Cochin backwaters conducted during the years 1971-72. This monsoonal estuarine system is particularly interesting, since it exhibits a wide range of variations in its environmental conditions which is naturally reflected in the fauna also. Several publications on various aspects of its hydrobiology have come out in the recent past. But studies on the zooplankton of the estuary have mostly been discontinuous either in space or time or restricted to its groups

Studies On Chitinoclastic Bacteria In Coastal Zone

The thesis is proposed to study the occurrence and distribution of chitinoclasts in water, sediment and fauna as related to site characteristics such as temperature, salinity, depth of water, pH, etc. Since no information is available on the chitinolytic properties of coastal strains, it is also proposed to study the chitinolytic activity of the bacterial isolates in relation to various environmental conditions. It is also planned to work out the taxonomy of some of the representative isolates and certain kinetic properties of their chitinases. It is expected that the results of the study would yield a comprehensive information-on the chitinoclastic bacteria in the southern coastal zone of west coast of India.

“Population Characteristics, Fishery and Post larval distribution of Macrobrachium rosenbergii (de Man) and M. idella (Hilgendorf) of Vembanad lake”

Macrobrachium rosenbergii (de Man) and M.ide77a (Hilgendorf) form two commercially important Palaemonid prawns of the Vembanad lake (9 28' and 10 10’ N and 76 13' and 76 31' E). Both of them were known to have contributed to a very lucrative fishery during the sixties, however, in recent years these natural resources have badly depleted owing to the impact of many man made alterations brought about in the ecosystem such as habitat reduction, physical obstruction imposed in the migratory pathway of these species, pollution hazards, etc,. Changed environmental conditions and increased fishing pressures caused persistent alterations in the stock size of these prawns during the past so many years, however, no serious attempt was made to monitor the stock size from time to time and also to bring out the resource characteristics. Though, the morphotypic differentiation in grow out male population of M.rosenbergii has been documented, no similar studies were conducted with regard to natural male and female population. Based on the data collected during fishery cruise surveys conducted in Vembanad lake from March '94 to February '96, population characteristics. postlarval distribution, fishery and population dynamics of M.rosenbergii and M.ide77a of the lake were studied in detail.

Studies on the toxic effects of selected heavy metals in the freshwater mussel lamellidens corrianus (lea)

Industrialisation affects air, water, and soil. Industrial effluents which enter the aquatic environment either by direct disposal or through run off, affect living organisms at morphological and physiological levels. In any living tissue toxic materials exert their effects first at molecular and biochemical levels (Robbins and Angell, 1976). Most of the industrial effluents contain elevated concentrations of organic and inorganic chemicals capable of eliciting stimulatory or inhibitory effects on the metabolism of aquatic organisms. Heavy metals form an important group of environmental pollutants. Effects of pollution on the aquatic environment by heavy metals have received considerable attention in recent years due to their toxicity even at very low levels, persistence in the environment, and chances of getting biomagnified. A pollutant that does not affect a particular process under normal unstressed condition may affect the ability of the animal to adjust to changing environmental conditions which ultimately decrease its chances of survival (Thurberg et al., 1973

Studies on nitrifying microorganisms in Cochin Estuary and adjacent coastal waters

This thesis entitled “Studies on Nitrifying Microorganisms in Cochin Estuary and Adjacent Coastal Waters” reports for the first time the spatial andtemporal variations in the abundance and activity of nitrifiers (Ammonia oxidizingbacteria-AOB; Nitrite oxidizing bacteria- NOB and Ammonia oxidizing archaea-AOA) from the Cochin Estuary (CE), a monsoon driven, nutrient rich tropicalestuary along the southwest coast of India. To fulfil the above objectives, field observations were carried out for aperiod of one year (2011) in the CE. Surface (1 m below surface) and near-bottomwater samples were collected from four locations (stations 1 to 3 in estuary and 4 in coastal region), covering pre-monsoon, monsoon and post-monsoon seasons. Station 1 is a low saline station (salinity range 0-10) with high freshwater influx While stations 2 and 3 are intermediately saline stations (salinity ranges 10-25). Station 4 is located ~20 km away from station 3 with least influence of fresh water and is considered as high saline (salinity range 25- 35) station. Ambient physicochemical parameters like temperature, pH, salinity, dissolved oxygen (DO), Ammonium, nitrite, nitrate, phosphate and silicate of surface and bottom waters were measured using standard techniques. Abundance of Eubacteria, total Archaea and ammonia and nitrite oxidizing bacteria (AOB and NOB) were quantified using Fluorescent in situ Hybridization (FISH) with oligonucleotide probes labeled withCy3. Community structure of AOB and AOA was studied using PCR Denaturing Gradient Gel Electrophoresis (DGGE) technique. PCR products were cloned and sequenced to determine approximate phylogenetic affiliations. Nitrification rate in the water samples were analyzed using chemical NaClO3 (inhibitor of nitrite oxidation), and ATU (inhibitor of ammonium oxidation). Contribution of AOA and AOB in ammonia oxidation process was measured based on the recovered ammonia oxidation rate. The contribution of AOB and AOA were analyzed after inhibiting the activities of AOB and AOA separately using specific protein inhibitors. To understand the factors influencing or controlling nitrification, various statistical tools were used viz. Karl Pearson’s correlation (to find out the relationship between environmental parameters, bacterial abundance and activity), three-way ANOVA (to find out the significant variation between observations), Canonical Discriminant Analysis (CDA) (for the discrimination of stations based on observations), Multivariate statistics, Principal components analysis (PCA) and Step up multiple regression model (SMRM) (First order interaction effects were applied to determine the significantly contributing biological and environmental parameters to the numerical abundance of nitrifiers). In the CE, nitrification is modulated by the complex interplay between different nitrifiers and environmental variables which in turn is dictated by various hydrodynamic characteristics like fresh water discharge and seawater influx brought in by river water discharge and flushing. AOB in the CE are more adapted to varying environmental conditions compared to AOA though the diversity of AOA is higher than AOB. The abundance and seasonality of AOB and NOB is influenced by the concentration of ammonia in the water column. AOB are the major players in modulating ammonia oxidation process in the water column of CE. The distribution pattern and seasonality of AOB and NOB in the CE suggest that these organisms coexist, and are responsible for modulating the entire nitrification process in the estuary. This process is fuelled by the cross feeding among different nitrifiers, which in turn is dictated by nutrient levels especially ammonia. Though nitrification modulates the increasing anthropogenic ammonia concentration the anthropogenic inputs have to be controlled to prevent eutrophication and associated environmental changes.

Provenance, Isolation and Characterisation of Organic Matter in the Cochin Estuarine Sediment-“a Diagenetic Amino Acid Marker Scenario

Cochin estuarine system is among the most productive aquatic environment along the Southwest coast of India, exhibits unique ecological features and possess greater socioeconomic relevance. Serious investigations carried out during the past decades on the hydro biogeochemical variables pointed out variations in the health and ecological functioning of this ecosystem. Characterisation of organic matter in the estuary has been attempted in many investigations. But detailed studies covering the degradation state of organic matter using molecular level approach is not attempted. The thesis entitled Provenance, Isolation and Characterisation of Organic Matter in the Cochin Estuarine Sediment-“ A Diagenetic Amino Acid Marker Scenario” is an integrated approach to evaluate the source, quantity, quality, and degradation state of the organic matter in the surface sediments of Cochin estuarine system with the combined application of bulk and molecular level tools. Sediment and water samples from nine stations situated at Cochin estuary were collected in five seasonal sampling campaigns, for the biogeochemical assessment and their distribution pattern of sedimentary organic matter. The sampling seasons were described and abbreviated as follows: April- 2009 (pre monsoon: PRM09), August-2009 (monsoon: MON09), January-2010 (post monsoon: POM09), April-2010 (pre monsoon: PRM10) and September- 2012 (monsoon: MON12). In order to evaluate the general environmental conditions of the estuary, water samples were analysed for water quality parameters, chlorophyll pigments and nutrients by standard methods. Investigations suggested the fact that hydrographical variables and nutrients in Cochin estuary supports diverse species of flora and fauna. Moreover the sedimentary variables such as pH, Eh, texture, TOC, fractions of nitrogen and phosphorous were determined to assess the general geochemical setting as well as redox status. The periodically fluctuating oxic/ anoxic conditions and texture serve as the most significant variables controlling other variables of the aquatic environment. The organic matter in estuary comprise of a complex mixture of autochthonous as well as allochthonous materials. Autochthonous input is limited or enhanced by the nutrient elements like N and P (in their various fractions), used as a tool to evaluate their bioavailability. Bulk parameter approach like biochemical composition, stoichiometric elemental ratios and stable carbon isotope ratio was also employed to assess the quality and quantity of sedimentary organic matter in the study area. Molecular level charactersation of free sugars and amino acids were carried out by liquid chromatographic techniques. Carbohydrates are the products of primary production and their occurrence in sediments as free sugars can provide information on the estuarine productivity. Amino acid biogeochemistry provided implications on the system productivity, nature of organic matter as well as degradation status of the sedimentary organic matter in the study area. The predominance of carbohydrates over protein indicated faster mineralisation of proteinaceous organic matter in sediments and the estuary behaves as a detrital trap for the accumulation of aged organic matter. The higher lipid content and LPD/CHO ratio pointed towards the better food quality that supports benthic fauna and better accumulation of lipid compounds in the sedimentary environment. Allochthonous addition of carbohydrates via terrestrial run off was responsible for the lower PRT/CHO ratio estimated in thesediments and the lower ratios also denoted a detrital heterotrophic environment. Biopolymeric carbon and the algal contribution to BPC provided important information on the better understanding the trophic state of the estuarine system and the higher values of chlorophyll-a to phaeophytin ratio indicated deposition of phytoplankton to sediment at a rapid rate. The estimated TOC/TN ratios implied the combined input of both terrestrial and autochthonous organic matter to sedimentsAmong the free sugars, depleted levels of glucose in sediments in most of the stations and abundance of mannose at station S5 was observed during the present investigation. Among aldohexoses, concentration of galactose was found to be higher in most of the stationsRelative abundance of AAs in the estuarine sediments based on seasons followed the trend: PRM09-Leucine > Phenylalanine > Argine > Lysine, MON09-Lysine > Aspartic acid > Histidine > Tyrosine > Phenylalanine, POM09-Lysine > Histadine > Phenyalanine > Leucine > Methionine > Serine > Proline > Aspartic acid, PRM10-Valine > Aspartic acid > Histidine > Phenylalanine > Serine > Proline, MON12-Lysine > Phenylalanine > Aspartic acid > Histidine > Valine > Tyrsine > MethionineThe classification of study area into three zones based on salinity was employed in the present study for the sake of simplicity and generalized interpretations. The distribution of AAs in the three zones followed the trend: Fresh water zone (S1, S2):- Phenylalanine > Lysine > Aspartic acid > Methionine > Valine ῀ Leucine > Proline > Histidine > Glycine > Serine > Glutamic acid > Tyrosine > Arginine > Alanine > Threonine > Cysteine > Isoleucine. Estuarine zone (S3, S4, S5, S6):- Lysine > Aspartic acid > Phenylalanine > Leucine > Valine > Histidine > Methionine > Tyrosine > Serine > Glutamic acid > Proline > Glycine > Arginine > Alanine > Isoleucine > Cysteine > Threonine. Riverine /Industrial zone (S7, S8, S9):- Phenylalanine > Lysine > Aspartic acid > Histidine > Serine > Arginine > Tyrosine > Leucine > Methionine > Glutamic acid > Alanine > Glycine > Cysteine > Proline > Isoleucine > Threonine > Valine. The abundance of AAs like glutamic acid, aspartic acid, isoleucine, valine, tyrosine, and phenylalanine in sediments of the study area indicated freshly derived organic matter.