Fusarium solani f. sp. passiflorae: A new forma specialis causing collar rot in yellow passion fruit

The aim of this study was to characterize a Fusarium population obtained from yellow passion fruit (YPF) with collar rot using pathogenicity, morphocultural characteristics and molecular tests. Pathogenicity and disease severity were assessed in six plant species: YPF, zucchini, tomato, bean, soya bean and cucumber. Potato dextrose agar medium (PDA) was used to determine mycelial growth at five temperatures (15-35°C). The colour produced by isolates was also determined on PDA at 25°C. Synthetic nutrient agar medium was used to evaluate: (i) type of mycelium and phialides; (ii) size, shape and number of septa from conidia; and (iii) production of chlamydospores and perithecia. Molecular tests consisted of sequencing the ITS-5·8S rDNA region and elongation factor 1α (EF-1α) gene. The isolates caused large lesions on YPF, zucchini and tomato, with YPF having the highest mean disease severity and being the only one that showed wilt symptoms and death of the plant. Thus the isolates showed host specificity. Maximum mycelial growth occurred at 25°C and the predominant colour was bluish-white. The isolates produced long phialides, dense aerial mycelium, oval microconidia with a mean size of 9·5 × 2·6 μm, macroconidia of 32·7 × 3·4 μm with 3·3 septa, and chlamydospores; only one isolate lacked perithecia. Phylogenetic trees of the ITS region and EF-1α gene showed that isolates from YPF formed a distinct group within the F. solani group and the formae speciales of F. solani. It is proposed to name all isolates from YPF as F. solani f. sp. passiflorae. © 2013 British Society for Plant Pathology.

Time-course morphological and functional disorders of the kidney induced by long-term high-fat diet intake in female rats

BackgroundEvidence is emerging that highlights the far-reaching consequences of a high-fat diet (HFD) on kidney morphology and function disorders.MethodsThe present study was performed on 3-, 5-, 7- and 9-week-old HFD female rats compared with the appropriate gender and age-matched animals. We evaluated the kidney expression of angiotensin type II receptor and fibrotic and epithelial-to-mesenchymal transition (EMT) markers, by immunoblotting and immunohistochemical and histological techniques, in parallel with kidney function.ResultsIn the current study, the time-course HFD-treated group showed, by immunoblotting and immunohistochemical analysis, an early time-course increase in the expression of transforming growth factor β-1 (TGFβ-1) in the entire kidney of HFD-treated rats, compared with that observed in the control group. Simultaneously, the study shows a transient increase in the expression of ZEB2 in the HFD whole kidney accompanied by a fall in the E-cadherin expression and increased collagen and fibronectin deposition. A pronounced decrease in fractional urinary sodium excretion was also demonstrated in the long-term HFD-treated rats. The decreased FENa + was accompanied by a fall in FEPNa + and FEPP Na +, which occurred in association with significantly decreased CCr and, certainly on the sodium-filtered load. The reduction in the glomerular filtration rate (GFR) occurred in parallel to proteinuria and glomerular desmin overexpression.ConclusionsThe results of the current study suggest that podocyte injury in parallel with observed proteinuria and evidence of EMT transformation are associated with long-term loss of kidney function and renal sodium and water retention. © 2013 The Author. All rights reserved.

Eficiência da azospirillum brasilense na fixação de nitrogênio em milho

Pós-graduação em Agronomia (Agricultura) - FCA

Identificação de fatores de transcrição de Neurospora crassa envolvidos na regulação do metabolismo de glicogênio

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Atributos químicos e biológicos de solos sob diferentes ecossistemas e adicionados de material vegetal de soja

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Perfil metabólico de cálcio e ósseo de pacientes soropositivos para HIV em uso ou não da terapia antirretroviral

Pós-graduação em Alimentos e Nutrição - FCFAR

Análise dos parâmetros clínicos periodontais e expressão genética de interferons alfa, gama e genes relacionados em indivíduos portadores de Síndrome de Down com doença periodontal

Pós-graduação em Ciências Odontológicas - FOAR

Informe de la reunión de expertos sobre Panamá en el proceso de integración centroamericana

1. A lo largo de las últimas décadas, se han dado pasos de acercamiento entre Panamá y el proceso de integración centroamericana. A lo largo de este proceso, Panamá ha participado en algunas reuniones de los organismos de la integración regional, ha firmado acuerdos comerciales con sus miembros y ha analizado el cómo y cuándo ingresar al proceso de integración centroamericana. Recientemente, como consecuencia del proceso de globalización y el establecimiento de la política de regionalismo abierto en la región, las posturas de los países centroamericanos y Panamá se han acercado como nunca antes. Atendiendo a estos cambios y a lo dispuesto en el programa de trabajo regular de la Unidad de Comercio Internacional e Industria (UCII); para 2008-2009, se ha llevado a cabo un estudio sobre "Panamá y el proceso de integración centroamericana", en el que se analizan algunas áreas relevantes que podrán acercar más a Panamá y al proceso de integración centroamericana, y se identifican algunas determinantes que han facilitado un vínculo más estrecho entre Panamá y Costa Rica. 2. La Reunión de Expertos sobre “Panamá en el proceso de integración centroamericana” responde al compromiso establecido en el Plan de trabajo regular de la CEPAL para 2008-2009 para llevar a cabo una reunión de expertos relativa al tema de integración y políticas comerciales en la subregión. Las sugerencias de los expertos citados en está reunión ayudaron a elaborar la versión final del documento Panamá y el proceso de integración centroamericana”. 3. Lo que sigue de este informe tiene como objetivo principal dar cuenta de la organización, dinámica y conclusiones de este evento. Report on the Meeting of Experts on Panama in the process of Central American integration Abstract: 1. During the greater part of the past decades, Panama has begun approaching the process of Central American integration. Throughout this process, Panama has participated in several meetings with the organizations focused on regional integration, and signed several trade agreements with their members, as well as analyzed how and when to join the process of Central American integration. Recently, as a consequence of globalization and establishment of regional policies of openness in the region, the positions of the countries of Central America and Panama have become closer aligned than ever before. In response to these changes and information over the regular work program of the Unit for International Trade and Industry (UCII); for 2008-2009, a study was carried out over the subject of Panama and the process of Central American integration, in which some areas relevant to the subject Panama and the process of Central American integration were analyzed, and determinants that have facilitated a stronger link between Panama and Costa Rica were identified. 2. The meeting of experts over “ Panama and the process of Central American integration”, was in response to commitment established in the regular work plan of ECLAC for 2008-2009 to carry out a meeting experts on the matters of integration and trade policies of the subregion. The suggestions of the experts quoted from this event, helped to elaborate the final version of the report “Panama and the process of Central American integration”. 3. The main objective of this report is to provide information over the organization, dynamics and conclusions of this event.

Avaliação do padrão de metilação de regiões promotoras dos genes ESR1, ESR2 e PGR na endometriose profunda intestinal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Variação da ocorrência da rinotraqueíte infecciosa bovina pela associação com a diarréia viral bovina e a leucose enzoótica bovina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção leiteira em cabras da raça Saanen: influência dos hormônios cortisol e IGF-I

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificação de proteínas ligantes de CRABP2, proteína envolvida na via de sinalização celular por ácido retinóico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Histologia e morfometria dos testículos de gatos domésticos adultos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de marcadores moleculares sobre características de crescimento e reprodutivas de bovinos da raça Canchim

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Silagem de grãos úmidos de sorgo para frangos de corte criados no sistema alternativo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)