Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of fluoride varnish supplemented with sodium trimetaphosphate on enamel erosion and abrasion: An in situ/ex vivo study

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Hymenaea stigonocarpa Mart. ex Hayne: A tropical medicinal plant with intestinal anti-inflammatory activity in TNBS model of intestinal inflammation in rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Orange Juice and Hesperidin Promote Differential Innate Immune Response in Macrophages ex vivo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Documentação de fé: reflexões sobre os ex-votos e a sala das promessas do Santuário Nacional de Aparecida

This article brings to light a reflection about the possibility to enlarge the notion about document. Beyond the French school explanation, and taking up again important author of Information Science campus, the debates exposes within the Brazilian Catholic dimensions is possible to extrapolate the revision about the document’s idea, which is generally restrict to informational spaces. The National Sanctuary of Aparecida protects in a specific room the votive offerings – objects leaved there whether to pay a promise or to thank for any miracle that someone believes receive it. It is mediation’ space and it is can be thought as an environment, because there are possibilities to establish mediations inside it. Although, the room of the promises is a sacred and profane place, it involves a Cathequesis mission, expositive, informational, in spite of this last characteristic does not be explicit. First of all, the article reflects about the room of the promises and follows analyzing the document’s comprehension; in a second moment we finish purposing to understand the votive offering as a faith’s document, because they assume the position as proof of miracles and received graces, and these special objects brings with them a materiality positive and a documental value which the room fo the promises give to them.

As passagens do sagrado: da mercadoria ao ex-voto no Santuário Nacional de Nossa Senhora Aparecida/Brasil

This article reflects on the votive objects exhibited in the room of promises situated in the National Sanctuary of Nossa Senhora da Conceição Aparecida, located in the city named Aparecida (São Paulo, Brazil). At the sanctuary, there is an area to receive and exhibit votive offerings, which are donated as payment or to initiate a relationship of faith. This article’s aim is to understand how, from entering the room of promises, the votive object is selected and receives new directions, within the Sanctuary or outside it. Nonetheless, a small number are kept in this specific room dedicated to votive offerings. These objects become documents, social agents, because they reveal stabilization within associative relationships. With the help of readings from Information Science and other areas, and after carrying out ethnographic research over three years, this article shows how the materiality of information is indispensable for apprehending the relationship between society and information. Through contact with the votive offerings, the visitor establishes inferences, interpretations, which assist in comprehending the space, faith, and Nossa Senhora da Conceição Aparecida.

Análise da atividade antimicrobiana dos extratos de Cordia verbenacea DC, Mikania laevigata Sch. Bip. ex Baker e Psidium Cattleianum frente a microrganismos endodônticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Propagação assexuada e desenvolvimento inicial de mudas propagadas sexuadamente de Ficus adhatodifolia Schott ex Spreng. (Moraceae)

Pós-graduação em Agronomia (Horticultura) - FCA

Investigação das atividades alelopática, fitotóxica e antioxidante de extratos e frações de Tridax procumbens L. (Asteraceae). e Ouratea spectabilis (mart. ex engl.) engl. (Ochnaceae)

Pós-graduação em Biociências - FCLAS

Expansão ex vivo de células uroteliais em scaffolds 3D

Malformations and possible damages to the urogenital system can be originated in the embryonic period. Moreover, fire guns, knives and accidents, where there is the disruption of the urethra, also cause these lesions. The objective was to analyze the contribution of tissue engineering in the construction of neo-urethra, developed by bioengineering. We performed an urothelial ex vivo expansion of cells in 3D scaffolds (platelet gel matrix and acellular porcine aorta) to assess the contribution of this technique in the construction of a neo-urethra. Mechanical dissociation was made of the inner wall of 10 North Folk rabbit’s bladder, weighing 2.5 to 3.0 kg. After dissociation the cell content was centrifuged and obtained a pellet of urothelial cells. The pellet was ressuspended in culture medium DMEM F12 and cells were maintained in culture for 15 days. Immunohistochemical analysis characterized the urothelial culture. The cells were then implanted in the scaffold - platelet gel. In a second experiment using aortic porcine acellular matrix were implanted urothelial cells alone and urothelial cells on platelet gel, on the inner wall of the scaffold - aorta, with space for setting bordered by a urethral probe. The complex probe - cells - aorta and probe - cells in platelet gel - aorta, were sealed with suture material and culture were maintained in a humidified 37ºC incubator with 5% CO2 in air for 12 days to subsequent histological analysis of urothelium cell adhesion to the scaffolds. By observation under an optical microscope, we could see the growth of cells in the scaffold platelet gel, from a monolayer in to a three-dimensional structure. In the acellular porcine aortic matrix containing the platelet gel, we could observe a few quantity of urothelial cells adhered. However with the acellular porcine aortic matrix in which was implanted only the urothelial cells, we have obtained adhesion to the wall

Efeito de reguladores vegetais no desenvolvimento de plantas jovens de Tabebuia avellanedae Lorentz ex Griseb

Tabebuia avellanedae Lorentz ex Griseb (ipê-roxo), Bignoniaceae, ocorre na Floresta Tropical Atlântica e é de grande importância, uma vez que, devido às suas propriedades medicinais e lenho de grande valor comercial, já esteve sob ameaça de extinção. A mistura de reguladores vegetais é conhecida como bioestimulante que pode incrementar o desenvolvimento vegetal, estimulando a divisão, a diferenciação e o alongamento celular. Assim, neste trabalho avaliou o efeito da aplicação da mistura de reguladores vegetais do grupo das auxinas, giberelinas e citocininas no desenvolvimento de plantas jovens de ipê-roxo. Os tratamentos foram: testemunha, GA4+7 + BAP 100 mg L-1, GA4+7 + BAP 200 mg L-1, GA3 + IBA + cinetina 0,5% e GA3 + IBA + cinetina 1,0% os quais foram aplicados 4 vezes em intervalos de 30 dias. O efeito dos tratamentos foi avaliado pelas medidas de altura das plantas, diâmetro do caule, número de folhas, massa seca das folhas, caule e raízes e avaliação de trocas gasosas. O tratamento com GA3 + BAP 200 mg L-1 foi aquele que apresentou maior efeito no crescimento das plantas. Já para as avaliações fotossintéticas o tratamento com GA3 + IBA + cinetina foi aquele que apresentou a maior taxa de assimilação de CO2

Avaliação do potencial antioxidante, citotóxico e fotoprotetor de extratos de Hymenaea courbaril L. e Hymenaea stigonocarpa Mart. ex Hayne

Pós-graduação em Biociências - FCLAS

Germinação de sementes e desenvolvimento inicial de Erythrina mulungu (Mart. ex. Benth)

The aim of this study was to determine the best pre-germination treatments for seeds and the best size container for seedlings growing of coral tree Erythrina mulungu (Mart. ex. Benth). Among the treatments applied to seeds, scarification with sulfuric acid during 10 minutes and cutting stylus in the region opposite to hilum resulted, respectively, in highest values of germination percentage (82.8% and 82.1%) and rates of germination speed (1.15 and 1.71). The biometric variables indicated no effect of container size on leaf area and dry mass plants. At 63 days after sowing, although with Dickson quality score statistically similar (mean 0.26), only plants grown in small and large bags reached the diameter collect close to 5 mm, minimum recommended for field planting. According to the results, for initial development of coral tree, it is recommended pre-seed treatment with sulfuric acid commercial, sowing in plastic bags of 17 x 20 cm, and wait 63 days before planting in the field.

Preliminary in vitro and ex vivo evaluation of afzelin, kaempferitrin and pterogynoside action over free radicals and reactive oxygen species

Biological activities of flavonoids have been extensively reviewed in literature. The biochemical profile of afzelin, kaempferitrin, and pterogynoside acting on reactive oxygen species was investigated in this paper. The flavonoids were able to act as scavengers of the superoxide anion, hypochlorous acid and taurine chloramine. Although flavonoids are naturally occurring substances in plants which antioxidant activities have been widely advertised as beneficial, afzelin, kaempferitrin, and pterogynoside were able to promote cytotoxic effect. In red blood cells this toxicity was enhanced, depending on flavonoids concentration, in the presence of hypochlorous acid, but reduced in the presence of 2,20 -azo-bis(2-amidinopropane) free radical. These flavonoids had also promoted the death of neutrophils, which was exacerbated when the oxidative burst was initiated by phorbol miristate acetate. Therefore, despite their well-known scavenging action toward free radicals and oxidants, these compounds could be very harmful to living organisms through their action over erythrocytes and neutrophils.

Alterações bioquímicas e visuais na vida pós-colheita de antúrio (Anthurium andraeanum Linden ex Andre) em resposta a aplicação de citocina

Pós-graduação em Ciências Biológicas (Botânica) - IBB