Atividade antimicrobiana de enxaguatórios bucais sem álcool à base de clorexidina sobre candida albicans

The aim of this study was to evaluate the antimicrobial activity of alcohol-free mouthwashes on Candida albicans. Twenty clinical isolates of C. albicans and one reference strain (ATCC 18804) were evaluated after exposure to two 0.12% chlorhexidine-based and alcohol-free (“Ca” and “Or”) in comparison to gluconate chlorhexidine with ethanol (positive control). The maximum inhibitory dilution (MID) and maximum fugal dilution (MFD) were determined by the microdilution method. Twelve serial dilutions (from 50 to 0.02%) were prepared in duplicate. Then, 100 µL of C. albicans suspension (106 cells.mL–1) were added to the wells. After incubation (37 °C/24 hours), MID was determined by reading the optical density. For MFD determination, the content of the wells were plated on Saouraud agar. For MID, there were no differences between groups Or and control, but Ca group showed a MID statistically higher (Kruskal-Wallis, p = 0.0012). For MFD, there were no differences between Ca and control (Mann-Whitney test, p = 0.1631). It can be concluded that Ca group showed a fungicid activity against C. albicans similar to the control, but lower fungistatic activity when compared to the control. Group Or showed only a fungistatic action similar to control.

Pesquisa de salmonella em mutuns (mitu mitu) mantidos em cativeiro

We collected 50 samples of curasow feces from the Scientific and Cultural Breeding Center in the city of Poços de Caldas. The samples were enriched in broth medium Tetrationato and Cystine-selenite and plated on Salmonella Shigella (SS), Mac Conkey (MC), endo-C (EC), Brilliant Green (VB) and Eosyn Methilen Blue EMB, remaining at 37 °C for 24h. Colonies suspected of Salmonella were inoculated in tubes containing Agar Triple Sugar Iron (TSI) and again incubated at 37 ºC for 24h. Tubes with characteristic growth were submitted to slide agglutination test with polyvalent somatic and flagellar serum. The medium SS, MC and VB were the most efficient for growing, and 36% of samples were positive for Salmonella spp. As birds are important reservoirs of Salmonella spp and it may represent a high risk to human health, there is a need to implement a cleaning routine in enclosures avoiding contamination between the enclosures and the consequent entrainment of these micro-organisms, by the keepers until their homes or other venues. The presence of Salmonella in breeding centers may be responsible for lower hatchability of eggs, undermining the purpose of the entity, which is to study and maintain the various birds species.

Efeito de nanofilmes depositados a plasma na resistência de união de um cimento resinoso a uma cerâmica à base de zircônia

Pós-graduação em Odontologia Restauradora - ICT

Biofilmes multiespécies de Candida Albicans associados com streptococcus mitis e streptococcus sanguinis: estudo in vitro e in vivo

Pós-graduação em Odontologia Restauradora - ICT

Fatores de virulência e infecção epitelial in vitro de biofilmes simples e mistos de Cndida albicans, Staphylococcus aureus sensível (MSSA) e resistente à meticilina (MRSA)

Pós-graduação em Reabilitação Oral - FOAR

Efeito da água ozonizada e gluconato de clorexidina sobre propriedades mecânicas e microbiológicas de materiais utilizados para confecção de próteses odontológicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Herbicide detection in groundwater in Córrego Rico-SP watershed

Due to the large amount of pesticides applied in agriculture, mainly herbicides, there is a growing concern about a possible environmental contamination with these products, including water bodies. Given the above, the aim of the present work was to detect and quantify herbicides through multiresidue analysis in water samples collected in semi-artesian wells and springs in a rural area of the city of Jaboticabal (SP). Samples were collected from 32 wells and 13 water springs, in three different seasons: October 2010, February 2011 and May 2011. Additionally, samples at a residence in the urban area were also collected. Analysis using high performance liquid chromatography coupled to mass spectrometry was performed and herbicides ametryn, amicarbazone, clomazone, diclosulan, diuron, hexazinone, imazapic, imazapyr, isoxaflutole, S-metolachlor, sulfentrazone, sulfometuron-methyl, and tebuthiuron were evaluated. In semi-artesian wells, an incresed quantity of herbicides was found in comparison with the water springs. Among the tested herbicides, hexazinone, imazapyr and sulfentrazone were detected in measurable amounts in accordance with the analytical method applied, while clomazone was the most common herbicide being detected in more than 60% of the samples. Ametryn, diuron and amicarbazone herbicides were also detected. Diclosulan, imazapic, isoxaflutole, S-metolachlor, sulfometuron-methyl, and tebuthiuron were not detected in any sample. Inappropriate use of these products without prior knowledge of the behavior of the soil can lead to groundwaters and water springs contamination, thus an ongoing monitoring of this resource becomes very important.

Antiseptic mouthwashes: in vitro antibacterial activity

Mouthwashes are used as an adjunct to tooth brushing for improving breath and preventing oral diseases. The aim of this study was to compare the in vitro Maximum Inhibitory Dilution (MID) of 3 mouthwashes with different active ingredients against mutans streptococci (MS). The products analyzed were PeriogardR, CepacolR and PlaxR Fresh Mint. Their antibacterial activity was assessed in duplicate in 96-well microtiter plates against 36 clinical isolates of MS. Each mouthwash was submitted to a serial two-fold dilution (1/2.5 to 1/5120) using double concentration of Tryptose Soy Broth with 1.0% yeast extract. The final volume in each well was 100 mL plus 5 mL of a bacterial suspension, equivalent to 107 CFU/mL. They were incubated microaerobically at 37oC for 48 hours and the MIDs determined. MID was 1/320 for PeriogardR and CepacolR, and 1/20 for PlaxR. Statistical analysis revealed that the MID of PeriogardR MID did not differ from that of CepacolR (p>0.05), and was higher than that of PlaxR (p<0.05). In conclusion, the antiseptic mouthwashes containing chlorhexidine (PeriogardR) and cetylpyridinium chloride (CepacolR) had higher in vitroantibacterial activity (MID) against MS than the antiseptic mouthwash containing triclosan (PlaxR), according to microbiological method employed.

Modelagem dos níveis freáticos do sistema aquífero Bauru (SAB) em diferentes usos da terra no município de Assis - SP

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Desafios tecnológicos na exploração de petróleo em águas profundas: o sistema PETROBRAS de inovação

Pós-graduação em Economia - FCLAR

Lógica Fuzzy aplicada à geologia

Pós-graduação em Matemática em Rede Nacional - IBILCE

O Instituto Cearense de Matemática (1954-1960): a origem do Instituto de Matemática da UFC

Pós-graduação em Educação Matemática - IGCE

Métodos geoelétricos aplicados ao diagnóstico ambiental subsuperficial do Cemitério Municipal de Rio Claro, SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação dos fatores críticos para a seleção e aplicação clínica dos pinos de fibra: relato de caso cliínico

Teeth that have lost much of their coronal tooth structure need intraradical devices to achieve a desired retention of the restorative material, restoring aesthetics and function to the patient. For this purpose, various types of intracanal posts have been studied, of wich the fiber posts have been highlighted with its increasing use in the dental market. The purpose of this study was to present a clinical case of using fiberglass post. As well as critically analyze the published works and points of controversy described by different authors related to factors such as different protocols cementation, surface treatments pins, fracture toughness and compression, the possibility of microleakage, diameter and length of the pin, and the various materials used in conjunction with the intracanal retainers. Thus, we concluded that fiber posts showed as an excellent alternative for anterior teeth, since have least 2 mm of tooth remaining.

Reabilitação estética com enfoque nas diferentes cores de substratos e na escolha da cor do cimento adesivo

The evolution of techniques and materials applied to adhesive ceramic restorations resulted in the intensification of its use, proving to be an excellent method of aesthetic restoration. Some important features of this technique are its conservative preparation, which avoids an excessive loss of tooth structure and its high aesthetic value provided by the ceramic even when utilized under different color of dental substrates. The aim of this paper was to report a clinical case with full crowns, porcelain veneers and non-prep veneers, enhancing peculiarities related to the material used, tooth preparation techniques, cementing, indications and contraindications.