A High Degree of LINE-1 Hypomethylation Is a Unique Feature of Early-Onset Colorectal Cancer

12 p.

Distribución de "coxiella burnetii" en los rumiantes domésticos y en la fauna silvestre de la Comunidad Atutónoma Vasca. Evaluacióndel efecto del tratamiento antibiótico y de la vacunación en el control de la fiebre Q en rebaños ovinos naturalmente infectados

243 p. : il.

Diário da Constituinte [gravação de vídeo] : [programa n. 661 1ª ed. ]

Líderes se reúnem para votar o novo texto do 2º turno, o líder do governo quer manter o destaque para votação em separado. os empresários defendem a nova Carta, eles se reuniram com o presidente Ulysses. Eles querem garantir os pontos da reserva de mercado e definição de empresa nacional

Influência da execução das emendas orçamentárias na reeleição parlamentar : análise da atuação dos deputados federais do Estado de Sergipe na 54ª legislatura (2011-2015)

Monografia (especialização) – Curso de Parlamento e Direito, Câmara dos Deputados, Centro de Formação, Treinamento e Aperfeiçoamento (Cefor), 2015.

O impacto das cotas eleitorais de gênero na representação feminina na Câmara dos Deputados [

Monografia (especialização) – Curso de Parlamento e Direito, Câmara dos Deputados, Centro de Formação, Treinamento e Aperfeiçoamento (Cefor), 2015.

Alvará com força de Lei, pelo qual ha Vossa Alteza Real por bem (por effeito dos augustos sentimentos da sua paternal affeição pelos seus fieis vassallos) mandar estabelecer nas Capitaes dos Governos, e Capitanias dos seus domínios ultramarinos Juntas, para resolver aquelles negocios, que antes se expedião pelo recurso à Meza do Desembargo do Paço, em grande detrimento das partes, pelas demoras, nocivas de longas, e pezadas despezas, que os vassallos de Vossa Alteza Real experimentavão, e soffrião no trato, e decisão das suas dependencias ; tudo na fórma acima declarada : para Vossa Alteza Real ver

Ao alto do título: Conde das Galveas.

Alvará, pelo qual V.A.R. ha por bem estabelecer hum methodo de Thesourarias Geraes para o Exercito, em que se reuna exactidão na fiscalização da Fazenda Real como prompto pagamento das tropas, extinguindo as Thesourarias, e Pagadorias, que agora existem, tudo como acima se declara : para Vossa Alteza Real ver

Variação do Título : Regulamento das Tesourarias Gerais do Exercito.

Alvará com força de Lei, pelo qual Vossa Alteza Real ha por bem dar o Regimento para regulação, e governo da relação, que foi servido mandar crear na cidade de S. Luiz do Maranhão ; tudo na fórma acima declarada : para Vossa Alteza Real ver

Referência: Bibliografia da Impressão Regia do Rio de Janeiro / Ana Maria de Almeida Camargo, Rubens Borba de Moraes, 1993. v. 2, p. 68.

Alvará, porque Vossa Alteza Real ha por bem crear hum Erario, e Conselho de Fazenda para a administração, arrecadação, distribuição, contabilidade, e assentamento do seu Real patrimonio, e fundos públicos deste Estado, e Domínios Ultramarinos, como nelle se declara : para Vossa Alteza Real ver

Ao alto do título : D. Fernando José de Portugal.

Alvará, pelo qual Vossa Alteza Real ha por bem crear huma Contadoria da Marinha, na fórma acima declarada : para Vossa Alteza Real ver

Ao alto do título: Visconde de Anadia.

A novel form of human disease with a protease-sensitive prion protein and heterozygosity methionine/valine at codon 129: Case report

Background: Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare neurodegenerative disorder in humans included in the group of Transmissible Spongiform Encephalopathies or prion diseases. The vast majority of sCJD cases are molecularly classified according to the abnormal prion protein (PrPSc) conformations along with polymorphism of codon 129 of the PRNP gene. Recently, a novel human disease, termed "protease-sensitive prionopathy", has been described. This disease shows a distinct clinical and neuropathological phenotype and it is associated to an abnormal prion protein more sensitive to protease digestion. Case presentation: We report the case of a 75-year-old-man who developed a clinical course and presented pathologic lesions compatible with sporadic Creutzfeldt-Jakob disease, and biochemical findings reminiscent of "protease-sensitive prionopathy". Neuropathological examinations revealed spongiform change mainly affecting the cerebral cortex, putamen/globus pallidus and thalamus, accompanied by mild astrocytosis and microgliosis, with slight involvement of the cerebellum. Confluent vacuoles were absent. Diffuse synaptic PrP deposits in these regions were largely removed following proteinase treatment. PrP deposition, as revealed with 3F4 and 1E4 antibodies, was markedly sensitive to pre-treatment with proteinase K. Molecular analysis of PrPSc showed an abnormal prion protein more sensitive to proteinase K digestion, with a five-band pattern of 28, 24, 21, 19, and 16 kDa, and three aglycosylated isoforms of 19, 16 and 6 kDa. This PrPSc was estimated to be 80% susceptible to digestion while the pathogenic prion protein associated with classical forms of sporadic Creutzfeldt-Jakob disease were only 2% (type VV2) and 23% (type MM1) susceptible. No mutations in the PRNP gene were found and genotype for codon 129 was heterozygous methionine/valine. Conclusions: A novel form of human disease with abnormal prion protein sensitive to protease and MV at codon 129 was described. Although clinical signs were compatible with sporadic Creutzfeldt-Jakob disease, the molecular subtype with the abnormal prion protein isoforms showing enhanced protease sensitivity was reminiscent of the "protease-sensitive prionopathy". It remains to be established whether the differences found between the latter and this case are due to the polymorphism at codon 129. Different degrees of proteinase K susceptibility were easily determined with the chemical polymer detection system which could help to detect proteinase-susceptible pathologic prion protein in diseases other than the classical ones.

The COMT Val158 Met polymorphism as an associated risk factor for Alzheimer disease and mild cognitive impairment in APOE 4 carriers

Background: The aim of this study is to examine the influence of the catechol-O-methyltranferase (COMT) gene (polymorphism Val158 Met) as a risk factor for Alzheimer's disease (AD) and mild cognitive impairment of amnesic type (MCI), and its synergistic effect with the apolipoprotein E gene (APOE). A total of 223 MCI patients, 345 AD and 253 healthy controls were analyzed. Clinical criteria and neuropsychological tests were used to establish diagnostic groups. The DNA Bank of the University of the Basque Country (UPV-EHU) (Spain) determined COMT Val158 Met and APOE genotypes using real time polymerase chain reaction (rtPCR) and polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLPs), respectively. Multinomial logistic regression models were used to determine the risk of AD and MCI. Results: Neither COMT alleles nor genotypes were independent risk factors for AD or MCI. The high activity genotypes (GG and AG) showed a synergistic effect with APOE epsilon 4 allele, increasing the risk of AD (OR = 5.96, 95% CI 2.74-12.94, p < 0.001 and OR = 6.71, 95% CI 3.36-13.41, p < 0.001 respectivily). In AD patients this effect was greater in women. In MCI patients such as synergistic effect was only found between AG and APOE epsilon 4 allele (OR = 3.21 95% CI 1.56-6.63, p = 0.02) and was greater in men (OR = 5.88 95% CI 1.69-20.42, p < 0.01). Conclusion: COMT (Val158 Met) polymorphism is not an independent risk factor for AD or MCI, but shows a synergistic effect with APOE epsilon 4 allele that proves greater in women with AD.

Diagnosis of invasive candidiasis by enzyme-linked immunosorbent assay using the N-terminal fragment of Candida albicans hyphal wall protein I

Background: The diagnosis of invasive candidiasis is difficult because there are no specific clinical manifestations of the disease and colonization and infection are difficult to distinguish. In the last decade, much effort has been made to develop reliable tests for rapid diagnosis of invasive candidiasis, but none of them have found widespread clinical use. Results: Antibodies against a recombinant N-terminal fragment of the Candida albicans germ tube-specific antigen hyphal wall protein 1 (Hwp1) generated in Escherichia coli were detected by both immunoblotting and ELISA tests in a group of 36 hematological or Intensive Care Unit patients with invasive candidiasis and in a group of 45 control patients at high risk for the mycosis who did not have clinical or microbiological data to document invasive candidiasis. Results were compared with an immunofluorescence test to detect antibodies to C. albicans germ tubes (CAGT). The sensitivity, specificity, positive and negative predictive values of a diagnostic test based on the detection of antibodies against the N-terminal fragment of Hwp1 by immunoblotting were 27.8 %, 95.6 %, 83.3 % and 62.3 %, respectively. Detection of antibodies to the N-terminal fragment of Hwp1 by ELISA increased the sensitivity (88.9 %) and the negative predictive value (90.2 %) but slightly decreased the specificity (82.6 %) and positive predictive values (80 %). The kinetics of antibody response to the N-terminal fragment of Hwp1 by ELISA was very similar to that observed by detecting antibodies to CAGT. Conclusion: An ELISA test to detect antibodies against a recombinant N-terminal fragment of the C. albicans germ tube cell wall antigen Hwp1 allows the diagnosis of invasive candidiasis with similar results to those obtained by detecting antibodies to CAGT but without the need of treating the sera to adsorb the antibodies against the cell wall surface of the blastospore.

Adequação do conteúdo do Plenarinho a seu público-alvo : 7 a 14 anos

Analisa o Plenarinho, um portal infantil da Câmara dos Deputados do Brasil, que visa contribuir para a formação cidadã de crianças de 7 a 14 anos. O principal desafio é a criação de produtos e conteúdos de modo que possam atrair este público.

As metas do Plano Nacional de Educação e o Plano Plurianual - 2016-2019

Busca a correlação entre as metas e iniciativas estabelecidas na proposta de Plano Plurianual PPA 2016-2019, nos termos do PL nº 06/2015-CN e o cumprimento das metas e estratégias estabelecidas no Plano Nacional de Educação (PNE), aprovado pela Lei nº 13.005, de 2014.