A nerve growth factor from the venom of Chinese cobra (Naja naja atra) and its effects on male reproductive system in rats

A nerve growth factor (NGF) was isolated from the venom of Chinese cobra (Naja naja ntr a) by ion exchange chromatography, gel filtration and fast protein liquid chromatography (FPLC). The N-terminal sequence of 22 amino acid residues was identical with other NGFs previously purified from the venom of the same genus. The NGF monomer molecular weight was estimated to be 13 500 by reducing SDS-PAGE and the isoelectric point was determined to be 7.2 by isoelectric focusing electrophoresis. NGF improved the epididymal sperm motility of male rats and increased the pregnancy rate and fetus number of mated female rats. The serum levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) of male rats administrated NGF + gossypol was lower than that of male rats administrated gossypol. Histological sections of testes and epididymides showed that NGF reduced the destructive effects of gossypol on rat testes. (C) 1999 Elsevier Science Inc. All rights reserved.

Study on regional differences in soil-water characteristics and their relevance to aquaculture productivity and fish disease

A total of 45 ponds used for fish polyculture were investigated in three zones of Bangladesh to identify the differences among the zones in respect to aqua-ecology, culture practices, fish productivity and health management. Four hundred and fifty fish from three zones were clinically examined by naked eye and histopathology. Out of total number of fish examined, 45 fish from Dhaka zones were examined for parasites and bacteria in addition to histopathology. Faded and haemorrhagic gill, skin, fin, scale loss and lesions were observed during fish examination. Aeromonas spp. Pseudomonas spp. and Streptococcus spp. were isolated respectively from 56%, 46% and 39% affected fish. Among the five water quality parameters analyzed, the highest average hardness and alkalinity respectively were recorded in Rajshahi (156 ppm and 142 ppm) followed by Dhaka (146 ppm and 132 ppm) and Chittagong (81 ppm and 90 ppm). The highest average pH was recorded in Mymensingh (7.52) followed by Rajshahi (7.13) and Chittagong (7.05). Water holding capacity of soil in Rajshahi zone was poor compared to other zones and farmers were found to be reluctant to fish farming.

Preservation of cotton fish net twines by tanning 1. Optimum concentration of tanning

Samples of tannin-containing preservatives used by fishermen in India for treating cotton nets were collected and qualitative and quantitative characterisation of the tannins made. The concentrations of different tannins required to impart optimum periods of preservation to the net were worked out and found to be 2% in 8 out of 10 materials studied.

Distribution of 5'-nucleotidase activity in the tissues of a freshwater teleost, Ophicephalus striatus Bloch

Homogenates of tissues have been analysed for the presence of 5'-nucleotidase activity. Sexes are not treated individually since no significant differences were observed. All organs showed marked activity, the highest being in the cardiac tissues, then the kidney, spleen, brain, and low values in the liver and muscles. Comparison with data from other fish studied shows a marked difference between O. striatus and O. punctatus.

Pickle curing of fish using tartaric acid and garlic as preservatives

Tartaric acid and garlic have proved highly satisfactory as pickling agents when used in combination, giving fishery products of good appearance, texture, taste and shelf life. Full details of the pickling method are given.

Utilization of the fish waste self-brine of fish curing industry as farm fertilizer

Large quantities of self-brine accumulating in curing tanks during the process of commercial fish salting is mostly wasted at present. This liquor exuded from the fish during the process of salting contains considerable amounts of soluble proteins and minerals. Due to the presence of organic matter the self-brine quickly putrefies causing nauseating smell. This renders the whole surroundings insanitary and often leads to health hazards to neighboring localities. Any economic method of utilising this waste brine and converting it into some useful product will be a tangible help to the fish curing industry. Hence, a method is given for converting this waste self-brine into a cheap and efficient fertilizer.

Digestion of selected foods by Yunnan snub-nosed monkey Rhinopithecus bieti (Colobinae)

Three digestion trials were conducted to quantify aspects of digestive physiology in the Yunnan snub-nosed monkey Rhinopithecus bieti, a foregut fermenter that feeds primarily on lichens. Mean retention time (MRT, the average time plastic markers spent in

Preliminary success on hormone induced captive breeding of goldspot mullet, Liza parsia (Ham.)

An attempt was made to breed goldspot mullet, Liza parsia in captivity through hormone induction. The fish started spawning 35-36 hours after a single dose of 2ml ova prim per kg body weight. Hatching of fertilized eggs completed within 42-48 hours after spawning. The mean hatching rate (%) was 71.33±12 corresponding to the fertilization rate (%) of 64±12. The larvae started its first external feeding on the third day and attained a length 2.5±0.25 mm. The salinity of both breeding and rearing cisterns was 20‰ and temperature was maintained at 22-23°C.

芒果扁喙叶蝉(Idioscopus incertus(Baker))生物学及其综合防治的研究

芒果扁喙叶蝉Idioscopus incertus (Baker)在云南元江干热河谷地区1年发生8-9代,世代重叠严重,以幼虫(若虫)、成虫越冬,越冬态不明显。4月中、下旬至5月上、中旬为第1个发生高峰期,是全年发生危害重、持续时间长的主要高峰期。7月下旬至8月上、中旬为第2个发生危害高峰期,对梢、叶的危害率分别为100%, 96.4%。采用综合防治措施后,其危害率分别降为4.7%,6.2%。

Cestodes in fish from a pond at Ile-Ife, Nigeria

The Farm Pond, University of Ife, was impounded by the construction of a dam, below the confluence of two small streams, between March and July, 1967. The pond is located at an altitude of 122.4 m. 0.D., its outline is an indented trapezium occupying 445 dkrn' at maximum fill. Natural food is fed into the pond by its two inlets, and the pond is often coloured a darkish brown due to the abundance of plankton. In 1969, on the recommendation by the Fishery Division, Ministry of Agriculture and Natural Resources, Ibadan, about four standard head-pans of poultry droppings per 40 dkm2 per month were spread uniformly over the pond during 1969 and 1970. Also four pounds of triple superphosphate per acre per month were spread for a period of six months. The pond was drained a number of times in 1970. However, the lowest average level of 1.37 m was recorded in April, 1970, before drainage began; and the maximum depth of 5.7 m was recorded in September and October, 1970.