Fontes proteicas para vacas leiteiras

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Resíduos agroindustriais para enriquecimento de fertilizantes orgânicos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito da adubação nitrogenada na produtividade do girassol

Nitrogen, along with K, is the most limiting nutrient for sunflower productivity. The objective of this research work was to determine the best level of nitrogen in a fertilization formula for the cultivation of sunflower of the ‘HELIO – 251’ cultivar. The fertilization procedure was that recommended for the state of São Paulo for sunflower crop, except that, in each one of the treatments, N doses were of 50, 70, 90, 110, and 130 kg ha-1 . These treatments were distributed in the field according to a randomized complete block design with 4 replications. The N dose which resulted in the highest grain yield, highest oil content, and plant dry matter was of 100 kg ha-1 . Leaf N content and 1,000 grains weight increased with N doses.

Fisioterapia respiratória, pressão intra-abdominal e função renal de pacientes de terapia intensiva

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Consumo de hidroxiprolina e amido e supersaturação urinária para oxalato de cálcio em gatos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Insuficiência de vitamina D em pacientes infectados pelo HIV tratados com terapia antirretroviral contendo tenofovir

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Insuficiência de vitamina D em pacientes infectados pelo HIV tratados com terapia antirretroviral contendo tenofovir

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Lovastatin protects mithochondrial and renal function in kidney ischemia-reperfusion in rats

PURPOSE: To investigate the effect of lovastatin on renal ischemia followed by reperfusion. METHODS: Thirty one Wistar rats submitted to left renal ischemia for 60 minutes followed by contralateral nephrectomy were divided into two groups: A (n = 17, control, no treatment), and B (n = 14, lovastatin 15 mg/kg/day p.o. ten days before ischemia). The animals were sacrificed at the end of ischemia, after 24 hours and at seven days after reperfusion. Survival, serum urea and creatinine levels and renal mitochondrial function were evaluated. RESULTS: Mortality was 29.4% in group A and 0.7% in group B. Urea and creatinine levels were increased in both groups, but the values were significantly lower in group B. Mitochondrial function showed decoupling in 83.4% of group A, as opposed to 38.4/% of group B. CONCLUSIONS: The result shows a protective action of renal function by lovastatin administered before ischemia/reperfusion. Since most of the mitochondrial fraction presented membranes with the ability to maintain ATP production in group B, stabilization of the mitochondrial membrane should be considered as part of the protective action of lovastatin on renal function in ischemia/reperfusion.

Estudo morfofuncional dos rins de cães da raça Golden Retriever afetados pela distrofia muscular (GRMD)

A Distrofia Muscular de Duchenne (DMD) é uma miopatia severa de caráter recessivo ligada ao cromossomo X e o modelo animal de estudo mais relevante é o Golden Retriever Muscular Dystrophy (GRMD). Além das severas alterações que ocorrem na musculatura estriada, muitos estudos mostram que outras estruturas, inclusive viscerais, podem se mostrar alteradas nesta patologia. Desta forma, este trabalho objetivou análisar e comparar possíveis alterações estruturais e funcionais do rim em cães GRMD. Neste modelo de estudo, foi possível observar a presença das faces convexa e côncava, do hilo renal e dos pólos craniais e caudais dos rins. O órgão mostrou-se envolto por uma cápsula fibrosa. Em um corte sagital do órgão, notou-se a presença das regiões cortical e medular e da pelve renal. Na análise microscópica foi possível identificar a zona medular e cortical com suas estruturas: os corpúsculos renais formados pelo glomérulo e pela cápsula de Bowman, os túbulos contorcidos proximais e distais, os ductos coletores, vasos sanguíneos e os segmentos das Alças de Henle. As dosagens séricas de creatinina e uréia encontram-se dentro dos limites de normalidade. Desta forma, de acordo com os nossos resultados, podemos concluir que os animais afetados estudados, não apresentaram alterações estruturais ou funcionais dos rins, o que nos permitir sugerir que apesar da ingestão hídrica comprometida, a estrutura renal, mantem- se preservada nos animais GRMD.

Nitrogen fertilizer (15N) leaching in a central pivot fertigated coffee crop

Nitrogen has a complex dynamics in the soil-plant-atmosphere system. N fertilizers are subject to chemical and microbial transformations in soils that can result in significant losses. Considering the cost of fertilizers, the adoption of good management practices like fertigation could improve the N use efficiency by crops. Water balances (WB) were applied to evaluate fertilizer N leaching using 15N labeled urea in west Bahia, Brazil. Three scenarios (2008/2009) were established: i) rainfall + irrigation the full year, ii) rainfall only; and iii) rainfall + irrigation only in the dry season. The water excess was considered equal to the deep drainage for the very flat area (runoff = 0) with a water table located several meters below soil surface (capillary rise = 0). The control volume for water balance calculations was the 0 - 1 m soil layer, considering that it involves the active root system. The water drained below 1 m was used to estimate fertilizer N leaching losses. WB calculations used the mathematic model of Penman-Monteith for evapotranspiration, considering the crop coefficient equal to unity. The high N application rate associated to the high rainfall plus irrigation was found to be the main cause for leaching, which values were 14.7 and 104.5 kg ha-1 for the rates 400 and 800 kg ha-1 of N, corresponding to 3.7 and 13.1 % of the applied fertilizer, respectively.

Movement of NH3 through the human urea transporter B: a new gas channel

Aquaporins and Rh proteins can function as gas (CO2 and NH3) channels. The present study explores the urea, H2O, CO2, and NH3 permeability of the human urea transporter B (UT-B) (SLC14A1), expressed in Xenopus oocytes. We monitored urea uptake using [14C]urea and measured osmotic water permeability (Pf) using video microscopy. To obtain a semiquantitative measure of gas permeability, we used microelectrodes to record the maximum transient change in surface pH (∆pHS) caused by exposing oocytes to 5% CO2/33 mM HCO3- (pHS increase) or 0.5 mM NH3/NH4+ (pHS decrease). UT-B expression increased oocyte permeability to urea by >20-fold, and Pf by 8-fold vs. H2O-injected control oocytes. UT-B expression had no effect on the CO2-induced ∆pHS but doubled the NH3-induced ∆pHS. Phloretin reduced UT-B-dependent urea uptake (Jurea * ) by 45%, Pf * by 50%, and (- ∆pHS * )NH3 by 70%. p-Chloromercuribenzene sulfonate reduced Jurea * by 25%, Pf * by 30%, and (∆pHS * )NH3 by 100%. Molecular dynamics (MD) simulations of membrane-embedded models of UT-B identified the monomeric UT-B pores as the main conduction pathway for both H2O and NH3 and characterized the energetics associated with permeation of these species through the channel. Mutating each of two conserved threonines lining the monomeric urea pores reduced H2O and NH3 permeability. Our data confirm that UT-B has significant H2O permeability and for the first time demonstrate significant NH3 permeability. Thus the UTs become the third family of gas channels. Inhibitor and mutagenesis studies and results of MD simulations suggest that NH3 and H2O pass through the three monomeric urea channels in UT-B.

Age, Sex, and Telomere Dynamics in a Long-Lived Seabird with Male-Biased Parental Care

The examination of telomere dynamics is a recent technique in ecology for assessing physiological state and age-related traits from individuals of unknown age. Telomeres shorten with age in most species and are expected to reflect physiological state, reproductive investment, and chronological age. Loss of telomere length is used as an indicator of biological aging, as this detrimental deterioration is associated with lowered survival. Lifespan dimorphism and more rapid senescence in the larger, shorter-lived sex are predicted in species with sexual size dimorphism, however, little is known about the effects of behavioral dimorphism on senescence and life history traits in species with sexual monomorphism. Here we compare telomere dynamics of thick-billed murres (Uria lomvia), a species with male-biased parental care, in two ways: 1) cross-sectionally in birds of known-age (0-28 years) from one colony and 2) longitudinally in birds from four colonies. Telomere dynamics are compared using three measures: the telomere restriction fragment (TRF), a lower window of TRF (TOE), and qPCR. All showed age-related shortening of telomeres, but the TRF measure also indicated that adult female murres have shorter telomere length than adult males, consistent with sex-specific patterns of ageing. Adult males had longer telomeres than adult females on all colonies examined, but chick telomere length did not differ by sex. Additionally, inter-annual telomere changes may be related to environmental conditions; birds from a potentially low quality colony lost telomeres, while those at more hospitable colonies maintained telomere length. We conclude that sex-specific patterns of telomere loss exist in the sexually monomorphic thick-billed murre but are likely to occur between fledging and recruitment. Longer telomeres in males may be related to their homogamous sex chromosomes (ZZ) or to selection for longer life in the care-giving sex. Environmental conditions appeared to be the primary drivers of annual changes in adult birds.

Las concepciones de los actuales alumnos sobre estructura de la materia

En este trabajo se expone las ideas de los alumnos que están cursando 5to y 6to año del nivel medio sobre estructura de la materia y estructura atómica, comparándolas con las concepciones alternativas reconocidas en investigaciones didácticas sobre la temática. Con el propósito de construir un panorama que responda a los siguientes interrogantes ¿La actual educación en ciencia ha podido generar instancias de reconstrucción de las concepciones alternativas de los estudiantes? ¿Las concepciones alternativas de los jóvenes que cursan la secundaria en nuestra región se diferencian de las reconocidas por las distintas investigaciones didácticas?. Se concretó una investigación del tipo descriptiva, utilizando un cuestionario con diferentes actividades validadas por distintos investigadores. Este estudio realiza aporte sobre tres aspectos relacionados: las concepciones de los estudiantes encuestados, cuestiones metodológicas para acercarnos al conocimiento que construyen los alumnos y aspectos para pensar la enseñanza. En referencia a las concepciones de los actuales estudiantes se puede afirmar que son semejantes a las concepciones encontradas en las investigaciones desarrolladas con anterioridad. Además, son ideas inconexas y contradictorias sobre estructura de la materia principalmente a nivel atómico

Las concepciones de los actuales alumnos sobre estructura de la materia

En este trabajo se expone las ideas de los alumnos que están cursando 5to y 6to año del nivel medio sobre estructura de la materia y estructura atómica, comparándolas con las concepciones alternativas reconocidas en investigaciones didácticas sobre la temática. Con el propósito de construir un panorama que responda a los siguientes interrogantes ¿La actual educación en ciencia ha podido generar instancias de reconstrucción de las concepciones alternativas de los estudiantes? ¿Las concepciones alternativas de los jóvenes que cursan la secundaria en nuestra región se diferencian de las reconocidas por las distintas investigaciones didácticas?. Se concretó una investigación del tipo descriptiva, utilizando un cuestionario con diferentes actividades validadas por distintos investigadores. Este estudio realiza aporte sobre tres aspectos relacionados: las concepciones de los estudiantes encuestados, cuestiones metodológicas para acercarnos al conocimiento que construyen los alumnos y aspectos para pensar la enseñanza. En referencia a las concepciones de los actuales estudiantes se puede afirmar que son semejantes a las concepciones encontradas en las investigaciones desarrolladas con anterioridad. Además, son ideas inconexas y contradictorias sobre estructura de la materia principalmente a nivel atómico

Las concepciones de los actuales alumnos sobre estructura de la materia

En este trabajo se expone las ideas de los alumnos que están cursando 5to y 6to año del nivel medio sobre estructura de la materia y estructura atómica, comparándolas con las concepciones alternativas reconocidas en investigaciones didácticas sobre la temática. Con el propósito de construir un panorama que responda a los siguientes interrogantes ¿La actual educación en ciencia ha podido generar instancias de reconstrucción de las concepciones alternativas de los estudiantes? ¿Las concepciones alternativas de los jóvenes que cursan la secundaria en nuestra región se diferencian de las reconocidas por las distintas investigaciones didácticas?. Se concretó una investigación del tipo descriptiva, utilizando un cuestionario con diferentes actividades validadas por distintos investigadores. Este estudio realiza aporte sobre tres aspectos relacionados: las concepciones de los estudiantes encuestados, cuestiones metodológicas para acercarnos al conocimiento que construyen los alumnos y aspectos para pensar la enseñanza. En referencia a las concepciones de los actuales estudiantes se puede afirmar que son semejantes a las concepciones encontradas en las investigaciones desarrolladas con anterioridad. Además, son ideas inconexas y contradictorias sobre estructura de la materia principalmente a nivel atómico