Zoneamento geoambiental do município e região de Franca-SP

O município de Franca-SP situa-se na Província Geomorfológica das Cuestas Basálticas no nordeste paulista, a qual possui características ambientais próprias. De uma forma geral, a região de Franca enfrenta problemas ambientais como a suscetibilidade à erosão linear e laminar, aos movimentos de massa e ao assoreamento dos canais de drenagem. Estes problemas, por sua vez, induzem a situações socioeconômicas, como o incremento da pobreza, aglomeração urbana e, uma emergente demanda da sociedade por mudanças no âmbito da gestão local. Assim, devem ser efetuados estudos detalhados sobre o meio, a fim de minimizar e/ou evitar tais problemas. Desta forma, propos-se a realização do Zoneamento Geoambiental, o que implica no levantamento, análise, interpretação e correlação dos diferentes componentes ambientais, visando dar subsídio a órgãos públicos e proprietários rurais, tanto na fase de planejamento como na implantação de culturas agrícolas, pecuária, reflorestamento e uso da água superficial (lençol freático), de maneira racional e ambientalmente sustentável, garantindo assim a ocupação adequada do solo e a melhor conservação dos recursos naturais. O trabalho iniciou-se com a organização e reinterpretação dos dados pré-existentes (topográficos, geológicos, pedológicos, geomorfológicos, morfoestruturais, de coberturas de alteração intempérica e sócio-econômicos) e geração e/ou reorganização de novos dados e propostas para a minimização dos impactos sócio-ambientais (rede de drenagem, hipsométricos, morfométricos, tectônicos, geológicos, geomorfológicos, climáticos e bióticos) com a utilização de técnicas de fotointerpretação de diversos sensores remotos e de campo. Deste modo, a correlação de todas as informações geoambientais relativas ao município proporcionou a delimitação das suas zonas e subzonas geoambientais... (Resumo completo, clicar acesso eletrônico abaixo)

A influência das características morfométricas e de uso da terra na morfodinâmica: um estudo da alta bacia do Rio Itanhaém - Baixada Santista/SP

Diante do desenfreado uso da terra, gerando impactos muitas vezes irreversíveis, o planejamento ambiental insere-se como uma ferramenta atualmente necessária. Visto que é sob o relevo que se consolidam as atividades antrópicas, a cartografia do relevo surge como um importante instrumento, indicando setores cujas características naturais impõem limites ao uso da terra. Assim, o objetivo desta pesquisa é analisar as características geomorfológicas da alta bacia do Rio Itanhaém, com ênfase à questão morfográfica e morfométrica do relevo, avaliando também as modificações impostas a este em decorrência do uso da terra, contribuindo, portanto, com a gestão ambiental desta área. Para tanto, foram elaborados documentos cartográficos capazes de evidenciar as características do relevo que impõem limites ao uso antrópico, a saber: as cartas morfométricas (carta de declividade, carta de dissecação vertical, carta de dissecação horizontal e carta de energia do relevo), que são documentos que evidenciam a suscetibilidade potencial do relevo sob a influência da ação das águas e gravitacional; a carta geomorfológica (cenário de 1962), que possibilita a espacialização das formas do relevo, contribuindo para a análise da morfodinâmica; e as cartas de uso da terra (cenário de 1962 e 2000), que, em conjunto com os documentos já especificados, possibilitam a análise da influência da atuação antrópica sob o sistema relevo. De maneira geral, pôde-se detectar problemas na morfodinâmica da área de estudo, evidenciados pela presença de feições denudacionais localizadas, tais como sulcos erosivos, voçorocas, ravina e cicatrizes de escorregamentos, os quais vinculam-se ao potencial natural que o relevo da área de estudo possui ao desencadeamento de processos morfogenéticos; à dinamização de processos naturais pela atuação antrópica... (Resumo completo, clicar acesso eletrônico abaixo)

Aplicação do método AHP para hierarquização de condicionantes de corrida de massa

Among the flow, the flows have the highest destructive potential, may cause disasters with a very high number of victims, as recorded in the events Caraguatatuba (SP) and the Serra das Araras (RJ) in 1967; Valeu do Rio Figueira, in Timbé do Sul (SC) between 1995 and 1996; Vale do Itajaí in Santa Catarina, in 2008, Teresópolis, Petrópolis and Nova Friburgo (RJ), in December 2010 and January 2011. This project aimed to prioritize the main determinants of inducing processes of debris flows by applying the method of decision making AHP (Analytical Hierarch Process). Therefore, the following stages of work were defined: research of the major factors of debris flows, questionnaire design method for application of AHP expert on the subject, analysis of the results obtained from calculating the ratio of consistency and preparation of proposal for hierarchical of conditions for debris flows. From the surveys it was possible to establish a relationship of conditions according to the scale of work, defining three levels: regional (1:250,000 to 1:100,000), Semi-Detail (1:50,000 to 1:25,000) and Detail (1: 10,000 or higher). The factors found were: concentration time, the flood wave, rain, landslide susceptibility (slope), drainage and development in the deposition. The project analyzed the results obtained from the questionnaires, which allowed the ranking of the constraints, contributing to weight constraints in studies of multicriteria mapping of susceptibility to the occurrence of debris flows. The study showed that the most of the analyzed datas were inconsistent because of the different opinion of the judges

Análise de metilação do gene FOXE1 em carcinoma de células escamosas em cães: padronização metodológica

It is believed that epigenetic mechanisms such as DNA methylation are important for the tumorigenesis and maintenance of the altered state of tumor cells. DNA methylation occurs by the addition of a methyl group to carbon 5 of cytosine, catalyzed by the enzyme DNA methyl-transferase, which can change the expression of a gene, including the tumor suppressor genes. In human squamous cell carcinoma, several features have shown the etiological role of genes in tumor development. Among them, FOXE1 gene (forkhead box E1 - thyroid transcription factor) is presented with an important role in susceptibility to disease. Similarly the FOXE1 methylation pattern could alter the expression of this gene in dogs and predisposed to tumor on. Therefore, this study aims to investigate in dogs, the validity of the strategy employed in humans to analyze the FOXE1 methylation status. DNA extraction from fresh frozen tumoral samples was performed by Wizard Genomic® DNA Purification Kit. The methylation status was determined by MSP-PCR (methylation-specific polymerase chain reaction), using 2.0 ng of DNA treated with sodium bisulphate. One hundred micrograms of bisulphite-modified DNA was amplified using primers specific for either methylated or unmethylated DNA (primers sequences are available at http://pathology2.jhu.edu/pancreas/primer.pdf). The analysis of fragments was loaded on to 7% polyacrylamide gels and silver nitrate staining. In this stage of technical approach, 60% were FOXE1 hypermethylated. In conclusion, it was observed that the standard technique for assessing the methylation pattern of gene FOXE1 in humans can be used for the same evaluation in dogs. The correlation of these molecular data with clinical and histopathological parameters may have diagnostic and prognostic value and still be used as a tumor marker for therapeutic decision and surgical approach

Análise comparativa de concepções de cartografia geomorfológica aplicada a ambientes litorâneos

The intense urbanization in coastal environments can cause environmental changes due to the susceptibility of these areas. It is therefore of utmost importance to understand the environmental quality of coastal area and the geomorphological features are essential to make it possible, as well as the geomorphological maps that are used as an instrument to analyze the relief. However, there is no standardization among the geomorphological mapping techniques, since the proposals vary according to the taxonomy of the relief, the adopted scale and the objective of the researcher. This paper aims to carry out geomorphological mappings of sectors of the city of Mongaguá, in São Paulo state, according to the conceptions of Verstappen and Zuidam (1975) and Gustavsson, Kolstrup and Seijmonsbergen (2006), seeking to verify the possibilities and constraints that each one can provide for environmental management. Verstappen and Zuidam’s design (1975) is a classical international bibliography proposal indicating the symbols and colors that will be used to represent the relief according to the origin shape of the geomorphological features; Gustavsson, Kolstrup and Seijmonsbergen's conception (2006) is a latest international proposal which also uses symbols and colors that can be combined to represent various forms of relief, coming from different origins. The mappings in this research are although not presented as a mainstream map, but as a cartographic representation in which the symbols are superimposed on the digital ortophoto map to provide the reader an association between symbolism and the relief represented. The result is a comparative analysis among the symbols that were used in each mapping, in which is discussed what design is the most appropriate, considering the adopted scale and the geomorphological features of the mapped area

Influência do SNP T3801C CYP1A1 sobre os níveis de danos oxidativos no DNA de células da mucosa gástrica de pacientes infectados pelo H. Pylori

Helicobacter pylori (H. pylori) is a common gastric pathogen that has infected more than 50% of the population of the world and it has been associated with chronic gastritis, gastric ulcers, duodenal ulcer, and gastric cancer. Although, almost all infected people develop gastritis, there is a variety of clinical outcomes, and only a minority (<1%) of infected individuals develop gastric cancer. There are evidences which suggest that the chronic inflammatory reaction caused by the bacterial infection may be involved in the production of reactive oxygen species or reactive nitrogen species. It may lead to DNA damage, which together with the cellular response could lead to gene mutations, chromosomal aberrations characterizing genomic instability that may represent the early step in gastric carcinogenesis. The extent and severity of gastric mucosal inflammation, as well as the clinical outcome of the infection, depend on a number of factors, including the host genetic susceptibility such SNP T3801 CYP1A1, immune response, age at which the infection was acquired, environmental factors, especially dietary and bacterial virulence factors. Due to the risk of developing gastric cancer in humans infected by H. pylori, we used the Comet Assay to investigate the influence of the SNP T3801C CYP1A1 on levels of oxidative DNA damage in gastric epithelial cells. The study was conducted with biopsies from the gastric antrum and corpus of 103 H. pylori-infected patients and 24 uninfected control patients. Genotype of SNP T3801C CYP1A1 was determined by PCR-RFLP and DNA damage levels were measured in gastric mucosal cells from antrum and corpus by the Comet assay. Levels of DNA damage in gastric mucosa cells from antrum and corpus of H. pylori-infected patients with mild, moderate, severe gastritis, and gastric cancer were significantly higher compared to uninfected normal mucosa cells. However, levels... (Complete abstract click electronic access below)

Análise de patologias de estradas vicinais para proposta de intervenção mínima visando a sua perenização

The unpaved back roads, despite their simplicity, are of paramount importance to the municipalities that their economy basis are in agri-livestock and a large portion of their population lives in rural areas, as it is from these pathways that flow of production is performed and ensuring access of the rural population to urban centers is allowed. Aiming the importance of these rural roads, this work studies the case of pathologies found in local roads of São Luiz do Paraitinga, located in the Vale do Paraíba upstate São Paulo, due to lack of a periodic maintenance program the roads not withstood the heavy rains late in the year 2009 and early 2010, generating major impacts on society and the economy of the city. From surveys conducted in the field, it was analyzed the various pathologies found (pathology drainage, slope and platform) checking also the nature of their occurrence, therefore propose solutions for intervention in rural roads, to decrease the susceptibility of these roads during the rainy season

Estudo da atividade antibacteriana in vitro de ativos desodorantes em solução hidroalcoólica

As estruturas da derme como os folículos pilosos e os ductos das glândulas sudoríparas e de óleo, proporcionam uma via de passagem por onde os micro-organismos atingem os tecidos mais profundos da pele. A transpiração fornece umidade e alguns nutrientes para o crescimento microbiano (TORTORA, 2012). Dentre os vários gêneros de bactérias que constituem a microbiota residente das axilas, encontram-se o Staphylococcus e o Micrococcus, responsáveis pelo odor do ácido isovalérico, o odor caracterísitico das axilas. Estão presentes também bactérias do gênero Corynebacterium, responsáveis pelo odor axilar pungente dos esteróides androgênicos (JACKMAN & NOBLE, 1983). O método mais eficaz de inibir a produção de exoenzimas bacterianas responsáveis pelo mau odor axiliar são as composições desodorantes. O método consiste em produzir um veículo cosmético (emulsão, suspensão em gás, composição hidroalcoólica, entre outros) e adicionar um ativo desodorante dissolvido ou em suspensão, que tem como propriedade a inibição da produção das exoenzimas bacterianas que levam à produção dos cheiros desagradáveis. (EIGEN & FROEBE, 1997). Neste trabalho testamos a suscetibilidade de três espécies de bactérias semelhantes às que compõem a microbiota permanente das axilas (Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 12228 e Corynebacterium xerosis ATCC 373), frente a três formulações cosméticas hidroalcoólicas de desodorantes em spray contendo ativos desodorantes. Utilizamos também a linhagem Escherichia coli ATCC 8739, pois esta enterobactéria pode estar presente nas axilas principalmente pelo contato destas com as mãos (BORICK & SARRA, 1960). A metodologia utilizada para os testes foi baseada na norma M7-A7 (CLSI, 2006), que trata dos testes de sensibilidade a agentes antimicrobianos por diluição para bactérias de crescimento aeróbico. A fórmula desodorante que teve melhor desempenho sobre...

Análise das ocorrências de escorregamento por meio de imagens Geoeye em trecho da faixa de dutos Rio de Janeiro - Belo Horizonte

The analysis of natural processes, such as landslides, has a great importance for the prevention of accidents in pipelines, since it might compromise its security, with serious social and environmental implications. Considering the large territorial dimensions that pipelines can reach, remote sensing tools have great applicability for data acquisition, allowing the analysis of large areas in regular time periods. This study aims to analyze the relations between the occurrence of landslides and its natural conditionings, on a stretch of the ORBEL pipeline, in the municipalities of Duque de Caxias, Nova Iguaçu and Belford Roxo – Rio de Janeiro. GeoEye sensor system images were used with spatial resolution of 0.5 m, from November/2009, January/2010 and March/2010, that are months of summer rains. The work involved the characterization of the study area and existing landslides, through bibliographic data collection; the application of visual analysis techniques for the extraction of landslide scars and fractures; preparation of maps for the landslide constraints and preparation of the susceptibility map. In the images from November only three landslide scars were extracted, while in January there were 166 and 111 in March. Difficulties were found with the shading in the images. From the relations among landslide scars, lithological units, relief systems, slope, downhill profile and the generated map of landslide susceptibility, presented in 1:70,000 scale, it was possible to conclude that slope is the main determinant of the process, allied secondarily to downhill profile, relief systems and lithology

Influência dos antígenos de histocompatibilidade na incidência de leucemia na região de Campinas, SP

Foi investigada a freqüência dos antígenos HLA-A, B, DR, DQ em 237 pacientes com vários tipos de leucemia, candidatos ao transplante de células tronco hematopoiéticas. O grupo controle foi constituído de 20.933 indivíduos saudáveis, tipados e cadastrados no Registro Brasileiro de Doadores Voluntários de Medula Óssea (REDOME), ou candidatos a doadores de órgãos, pertencentes ao mesmo grupo étnico e região geográfica que os pacientes. Para a determinação dos antígenos HLA foi definido o equivalente sorológico da genotipagem por Citometria de Fluxo PCR-SSO (Reação em cadeia da Polimerase – Oligonucleotídeo Seqüência Específica) com baixa resolução (One Lambda, Canoga Park, CA, US). Foram encontradas associações de suscetibilidade para os antígenos HLA-B70, HLA-B71, HLADR9 e HLA-DR10 associados a LMC (Leucemia Mielóide Crônica); HLA-A32 e HLA-B12 associados a LLC (Leucemia Linfóide Crônica); HLA-A19 e HLA-DR10 associados a LLA (Leucemia Linfóide Aguda). Associações de proteção foram encontradas para HLA-A19 associado a LMA (Leucemia Mielóide Aguda) e LMC; HLA-B44 e HLA-DR6 associados a LMC; HLA-DQ6 associado a LLC.

Efeito das baixas concentrações de agentes antineoplásicos sobre linfócitos humanos de sangue periférico

Our previous studies have shown that low concentrations (noncytotoxics) of antineoplastic agents modulate positively the dendritic cells, favoring their in vitro maturation and improving their antigen presenting function. The effects on colorectal cancer cells (HCT-116) were also investigated and we have observed an increased immunogenicity and susceptibility to cytotoxic T cells. Thereby, this study aimed to investigate the effect of 5-fluorouracil (5-FU) an azacitidine (AZA), in minimum effective and noncytotoxic concentrations on lymphocytes of healthy donors. In this study we have analyzed the cytotoxic effect of drugs at these concentrations as well as the proliferative ability of lymphocytes. In vitro production of IL-10 and IFN-γ has been also evaluated. We have observed that low concentrations of those chemotherapeutic agents are not cytotoxic for lymphocytes. However, the minimum effective concentrations (5-FU: 0,410±0,088 e AZA: 0,757±0,233; p<0, 05) have reduced the cell number. Proliferative activity of allogeneic lymphocytes in a mixed reaction (MLR) was not affected by the treatment. The cytokine production was not affected by the treatments, either. In conclusion, low concentrations of 5-FU and AZA has no deleterious effects on human peripheral blood lymphocytes and seems to be safe for combinatory administration with DC vaccines

Qualidade e erodibilidade de latossolos sob mata e cultivo de cana-de-açúcar

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

The frequency of HLA-DRB1 polymorphisms in Brazilian Plasmodium vivax malaria patients and in blood donors from the Amazon Region

We evaluated the frequency of different HLA-DRB1 alleles in Plasmodium vivax-infected individuals and in healthy blood donors from malaria endemic areas of Brazil. Low-resolution human leukocyte antigen-DRB1 genotyping was performed for 73 malaria patients and 29 healthy blood donors. The most frequent alleles in individuals from northern Brazil were human leukocyte antigen-DRB1*04, *08, *07 and *13. The frequency of human leukocyte antigen-DRB1*07 was higher in malaria-infected individuals than in the control group, which reinforces the theory that this allele plays an important role in susceptibility to malaria. This study offers new information about a potential susceptibility factor for P. vivax malaria in a Brazilian population that is naturally exposed to malaria.

Ocorrência, sorotipagem e rsistência antimicrobiana de Streptococcus suis em funcionários e ambente de abatedouro de sínos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação silício e alumínio em plantas de arroz de terras altas e mamona

Pós-graduação em Agronomia (Agricultura) - FCA