In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Kinetic role of a histidine residue in the T1 copper site of the laccase from Rigidoporus lignosus

Laccases (benzendiol:oxygen oxidoreductases; EC 1.10.3.2) catalyze the oxidation of a broad range of substrates, such as polyphenols, dyes and pollutants, and thus these enzymes are widely applied in industrial, biotechnological and environmental fields. In order to improve their biotechnological applications, a deep knowledge of structural factors involved in controlling their activity, in various experimental conditions and on different substrates, is required. In the present study, a laccase from the mushroom Rigidoporus lignosus was kinetically characterized. In particular, the stability, the effects of pH, ionic strength and fluoride ion concentration on the kinetic parameters were investigated, using three di-hydroxy-benzene isomers (1,2-dihydroxy-benzene, 1,3-dihydroxy-benzene and 1,4-dihydroxy-benzene) as substrates. The catalytic constant values of the laccase showed a bell-shaped pH profile, with the same optimum pH and pK(a) values for all tested substrates. This behavior appears to be due to the presence of an ionizable residue in the enzyme active site. To identify this residue, the enzyme was derivatized with diethylpyrocarbonate to modify accessible histidine residues, which, according to structural data, are present in the active site of this enzyme. The kinetic behavior of the derivatized laccase was compared with that of the native enzyme and the derivatized residues were identified by mass spectrometry. Mass spectrometry and kinetic results suggest the main role of His-457 in the control of the catalytic activity of laccase from R. lignosus. (C) 2013 Elsevier B.V. All rights reserved.

Kin-bias, breeding site selection and female fitness in a cannibalistic Neotropical frog

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of a co-substituted A-site on structural characteristics and ferroelectricity of (Pb, Ba, Ca)TiO3 complex perovskites: analysis of local-, medium- and long-range order

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Futebol e a construção da imagem de treinadores pela mídia: um estudo a partir das notícias de um site de grande visitação na web

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Primary relapse site pattern in women with triple-negative breast cancer

Despite the remarkable improvements in breast cancer (BC) characterization, accurate prediction of BC clinical behavior is often still difficult to achieve. Some studies have investigated the association between the molecular subtype, namely the basal-like BC and the pattern of relapse, however only few investigated the association between relapse pattern and immunohistochemical defined triple-negative breast cancers (TNBCs). The aim of this study was to evaluate the pattern of relapse in patients with TNBC, namely the primary distant relapse site:One-hundred twenty nine (129) invasive breast carcinomas with follow-up information were classified according to the molecular subtype using immunohistochemistry for ER, PgR and Her2. The association between TNBC and distant relapse primary site was analyzed by logistic regression. Using multivariate logistic regression analysis patients with TNBC displayed only 0.09(95% CI: 0.00-0.74; p = 0.02) the odds of, the non-TNBC patients of developing bone primary relapse. Regarding visceral and lymph-node relapse, no differences between in this cohort were found.Though classically regarded as aggressive tumors, TNBCs rarely development primary relapse in bone when compared to non-TNBC, a clinical relevant fact when investigating a metastasis of an occult or non-sampled primary BC. (C) 2014 Elsevier GmbH. All rights reserved.

Reproductive site selection in the leaf-frog Phyllomedusa azurea Cope, 1862 (Anura: Hylidae) in altered areas of the Brazilian Cerrado

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Site Selection for Restoration Planning: A Protocol With Landscape and Legislation Based Alternatives

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Relações públicas e reputação online: gerenciamento de crise no site americanas.com

The growing need for organizations to make themselves present on the Internet, the study aimed to demonstrate the importance of public relations activity in crisis management and analysis of the crisis in Americanas.com site. It also aims to analyze the electronic scene, highlighting their implications for consumer relations in e-commerce. In addition, as a consequence of conflicts, settles a loss situation, financial and reputation. A crisis begins with planning and communication failures, it is clear that public relations is essential in business today, as interest in integrating their knowledge and opinions, and maintaining a global view of the environment in which it operates. The case study analysis provided confirmation that the public relations face a growing market, which requires qualified and updated to do crisis management, especially in the online environment

CurtaBauru: site jornalístico especializado em cultura e arte na cidade de Bauru

CurtaBauru (www.curtabauru.com.br) is a journalistc website which specializes on the covering of local art and culture. The idea came up from the three author's interest in Bauru's cultural scene and also from the scarcity of local media about the subject. The website was opened in the end of August 2012, and its archives include journalistic covering of some major events such as Revirada Cultural 2012, Festival Canja de Artes Integradas and Encontro de Academias de Letras e Entidades Afins. Besides that, the website is updated daily with articles about coming up events, and includes weekly sections about people and organizations related to the city's artistic scene, restaurant criticism and an agenda containing the main events of the following week

Informatização na educação em saúde: avaliação de site educacional

Health education presently experiences the incorporation of information technology, thus making access to information more practical and attainable. The initiatives to make health care scientific content available virtually have shown to be useful for teaching, health care professionals and the general population. A website, however, must be constantly managed. To promote the dissemination of the website at www.educacaoemsaude.fmb.unesp.br among faculty members and undergraduate nursing students and students at Escola Técnica de Enfermagem (Vocational Nursing School) - ETE, by evaluating, among them, its graphic and functional aspects and seeking suggestions for its improvement. This is a descriptive quantitative study for which the participants were asked to fill out an instrument designed for the study. The evaluation was performed among nursing faculty members and students at Univ Estadual Paulista Júlio Mesquita Filho – UNESP and students at Escola Técnica de Enfermagem (Vocational Nursing School) – ETEC in the city of Botucatu. The number of participants totaled 88 individuals, of whom 23 were undergraduate students, 62 were students at ETEC, and 3 were faculty members. Eighty-one were females, and 7 were males. The participants’ age ranged from 17 to 56 years. Among the items that evaluated the website, the answers prevailed from “very good” to “good”. When the participants were asked what they had thought about the website, the answers were mostly positive, and the most frequently description given was “interesting”. Among the facilities, the most frequently reported was “easily understood content”, and as regards difficulties, handling the website was the most often mentioned. One hundred percent of the answers considered the maintenance of the website to be important. Suggestions for its improvement were given, and the most frequent... (Complete abstract click electronic access below)

O pequi no noticiário: análise dos resultados de uma pesquisa na busca do site Folha de S. Paulo

Among the fruit species of the Brazilian Cerrado, the pequi is much popular in regions where it is extracted. Often it used in typical dishes, on the industry medicinal, cosmetic and handmade, also it studied its potential to produce fuel. Thus, this article sought to ponder about the disclosure of this fruit in the press, by means of the search system's site Folha de S. Paulo and applying content analysis, proposed by Bardin, beyond bibliography and documentary research. The justification for this research is that the pequi is a fruit of great importance in the economy and sustainable, yet little is spoken about it in the mainstream media. The result is that, by the analysis, little is said about the pequi media and its focus, is principally, cultural journalism, in culinary.

Site and extent of amino acid digestion in dairy cattle fed with corn and its byproducts

The study was conducted to evaluated the site and extent of dry matter (DM), crude protein (CP), methionine (Met), lysine (Lys), and threonine (Thr) digestion of corn and byproducts obtained from corn germ mixed with different amounts of extruded or non-extruded ether extract (EE) in dairy cattle. Treatments consisted in eight types of feed and two processing in a 4 x 2 factorial design. There were four feeds: corn grain cracked (Corn), corn germ meal with 1% EE (CG1), corn germ meal with 7% EE (CG7), and corn germ meal with 10% EE (CG10). The feeds were processed in one of two ways: extruded (Ex) and not extruded. In situ techniques were used to determine DM, CP, Met, Lys, and Thr partial and total tract digestion. A basic diet was compounded of corn germ meal, soybean meal and coastcross hay in a 70: 30 roughage to concentrate ratio. There was no interaction (P>0.05) between feeds and processing method. Extrusion improved (P<0.05) total tract digestibility of corn DM but not CP. Intestinal digestibility was similar (P>0.05) for corn and corn germ meal mixed with 7 and 10% EE, regardless of EE processing method. The CP total tract digestibility of corn germ meal with 1% non-extruded EE was 16.62% higher (P<0.05) than that of the extruded form. The best total CP digestibility was obtained for corn germ meal with 7% EE, independently of the processing method. The effects of EE processing method on partial and total digestibility differed between amino acid. Corn and corn byproduct extrusion may improve dry matter digestibility, but do not necessarily influence crude protein digestion. Ruminal and intestinal digestibility of Met, Lys, and Thr depends on both feed type and processing method. Therefore, amino acid availability should be considered individually.

Impairment of neutrophil migration to remote inflammatory site during lung histoplasmosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Radarfacies and sedimentological analysis: study of sedimentary substrate from an archaeological site (shell mound), southern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)