666 resultados para Sêmen : Resfriamento


Relevância:

10.00% 10.00%

Publicador:

Resumo:

Este relatório de estágio descreve as atividades desenvolvidas no estágio curricular e desenvolve o tema ―Inseminação artificial em Python regius‖. O estágio de seis meses foi realizado na Exoclinic – clínica veterinária de aves e exóticos, permitindo consolidar conhecimentos através da prática clínica e agregar-lhes a área da medicina de animais exóticos, dedicando especial atenção à medicina de répteis. Desenvolvem-se os casos mais frequentes: medicina preventiva nas várias classes de animais, patologia dentária em roedores e lagomorfos, doença do bico e das penas dos psitacídeos e anorexia em répteis. Na monografia aborda-se a anatomofisiologia e o comportamento reprodutivos das cobras e a sua influência no maneio reprodutivo em cativeiro, métodos de avaliação de reprodutores e técnicas de inseminação artificial. Realizou-se um ensaio prático com seis animais, realizando colheitas de sémen, avaliação de alguns parâmetros do sémen, controlo folicular por ecografia e inseminação artificial. Apresentam-se os métodos, os resultados e respetiva discussão; Abstract: Exotic animal medicine This traineeship report describes the activities during the traineeship and develops the theme "Artificial insemination in Python regius." The six-month internship was held in Exoclinic - clínica veterinária de aves e exóticos, consolidating knowledge through clinical practice and adding the medical field of exotic animals, with special attention to reptiles. The most frequent clinical cases are developed: preventive medicine in the various classes of animals, dental pathology in rodents and lagomorphs, Psittacine Beak and Feather Disease in birds and anorexia in reptile. The monograph addresses the anatomy, physiology and reproductive behavior of snakes and their influence on reproductive management in captivity, methods for reproductors selection and evaluation and artificial insemination techniques. Implementation of a field trial with six animals, performing semen crops and evaluation of some of its parameters, follicular monitoring by ultrasound and artificial insemination. Methods and results are presented and discussed.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

Os produtos de controle biológico devem ser produzidos com custo competitivo em relação aos sintéticos para que seu uso se torne generalizado. A fermentação é uma das etapas mais caras para o escalonamento da produção por causa do valor dos ingredientes dos meios de cultura. Neste trabalho, Pichia kudriavzevii L9, um agente de controle de podridão pós-colheita de frutas, foi utilizada como modelo para a avaliação de hidrolisado de proteína de soja como fonte de nitrogênio em meio de cultura líquido. Foram testadas cinco concentrações de proteína de soja (50 L-1 a 250 g L-1) em solução aquosa contendo concentrações de H2SO4 de 0 a 1,0 N, tratadas em autoclave a 121 ºC e 1,0 atm de pressão. Após o resfriamento, o produto obtido foi alcalinizado com KOH até atingir o pH de 6,0 ? 7,0. Nas condições do experimento, a melhor condição para a hidrólise do proteinato de soja e obtenção de maior concentração de aminoácidos no meio, foi de 200 g L-1 de proteinato em solução de HCl 0,5 N, produzindo 32,1 g L-1 de aminoácido livre, quantificado pelo método da ninhidrina. O hidrolisado foi utilizado para o crescimento de P. kudriavzevii L9, obtendo-se a maior produtividade de células viáveis de levedura com a adição de 2% do hidrolisado obtido no meio de cultura, na faixa entre 0,2 a 0,5 N de HCL.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

The aim of this study was to correlate the testicular surface temperature with rectal temperature and semen quality in rams of different genotypic groups.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

One of the major causes of ?Fuyu? persimmon loss after cold storage (CS) is the breakdown of its flesh, which results in the production of a translucent fruit (a water-soaked fruit). It is believed that the cause of this disturbance is linked to disorganization of the cytoskelet and endomembrane system, which changes the synthesis and transport of proteins and metabolites, resulting in incomplete ripening. To test this hypothesis, ?Fuyu? persimmon was subjected to three different postharvest treatments (T): Control ? harvested and kept at 23±3 ◦C and relative humidity (RH) of 85±5% (room temperature, RT) for 12 days, T1 ? harvested and kept under cold storage (CS) (1±1 ◦C and RH of 85±5%) for 30 days followed by RT storage for 2 days, T2 ? kept under RT for 2 days (acclimatization) followed by CS for 30 days. Control and T2 resulted in fruit with decreased flesh firmness (FF), and increased soluble solids (SS) and ascorbic acid (AA) contents. In these fruit the activity of endo-1,4-ß-glucanase (endo-1,4-ß-gluc), pectin methylesterase (PME), polygalacturonase (PG) and ß-galactosidase (ß-gal) increased. T1 resulted in translucent fruit with decreased FF, without any enzymatic activity changes, probably due to the physical disruption of the cytoskeleton. Further, there was an increased content of proteins corresponding to expansins in fruit kept under Control and T2 conditions, which suggests that these conditions do contribute to the synthesis and/or transport of proteins involved in the process of solubilization of the cell wall. In these fruit, there was also a major accumulation of gene transcripts corresponding to heat shock proteins (HSPs) of organelles related to endomembrane, which suggests participation of these genes in the prevention of damage caused by cold conditions. These data proved the hypotheses that acclimatization contributes to the expression of HSPs, and synthesis and transportat of proteins involved in the solubilization of the cell wall. The expression of these genes results in the normal ripening of the persimmon, as confirmed by the evolution of ethylene production.

Relevância:

10.00% 10.00%

Publicador:

Resumo:

Pears have been grown in the south region of Brazil, where the climatic conditions are favourable. The aim of this work was to determine the harvest maturity index as well as maximum storage period of 'Packham's Triumph? and 'Rocha' pears to maintain quality attributes. The ?Packham?s Triumph? fruit were harvested from a commercial orchard at 7 days intervals and flesh firmness was used as a maturity index (MI1=76, MI2=67 and MI3=58 N). ?Rocha? pears were harvested twice and they were considered as MI1 and MI3 because of the firmness values. The fruit were stored at 1±1C and 90-95% RH for 15, 30, 45 and 60 days and evaluated at the end of each storage period and after five days at room temperature (24±1C), simulating a helflife period. Flesh firmness, water loss, peduncle dehydration, epidermis colour, soluble solids, titratable acidity were measured. ?Packham?s? pears harvested at MI1 and MI2 showed firmness loss after 30 days of cold storage, whereas fruit harvested at MI3 retained the initial values, resulting in firmer fruit after 60 days (P<0.001). Fruit harvested in MI3 had less firmness loss after 5 days at room temperature following 45 and 60 days of cold storage. ?Rocha? pears harvested in MI1 and MI3 showed firmness reduction during cold storage, which was intensified at room temperature. Maximum values of water loss approached 6%. Fruit peduncles of both cultivars dehydrated after 60 days of cold storage, but their colour remained green, independent of harvest maturity index. ?Packham?s Triumph? and ?Rocha? pears harvested at MI3 showed better quality attributes after 60 days of cold storage plus 5 days of shelf-life than fruit harvested at other maturity stages.