Agreed-upon procedures report on the City of Oxford, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Oxford, Iowa for the period July 1, 2013 through June 30, 2014

Agreed–upon procedures report on the City of Riverside, Iowa for the period July 1, 2013 through June 30, 2014

Agreed–upon procedures report on the City of Riverside, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Laurel, Iowa for the period February 1, 2014 through January 31, 2015

Agreed-upon procedures report on the City of Laurel, Iowa for the period February 1, 2014 through January 31, 2015

Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines

The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics.

Agreed-upon procedures report on the City of Ocheyedan, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Ocheyedan, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Collins, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Collins, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Martensdale, Iowa for the period November 1, 2013 through October 31, 2014

Agreed-upon procedures report on the City of Martensdale, Iowa for the period November 1, 2013 through October 31, 2014

Agreed-upon procedures report on the City of Urbana, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Urbana, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Sidney, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Sidney, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Glidden, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Glidden, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Fruitland, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Fruitland, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Walcott, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Walcott, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Pleasanton, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Pleasanton, Iowa for the period July 1, 2014 through June 30, 2015

Iowa Department of Administrative Services Central Procurement Enterprise (CPE) Policy and Procedures Manual, December 2013

Report on a review of the Central Procurement Enterprise (CPE) of the Iowa Department of Administrative Services for the period July 1, 2009 through March 31, 2013

Agreed-upon procedures report on the City of Dallas Center, Iowa for the period July 1, 2014 through June 30, 2015

Agreed-upon procedures report on the City of Dallas Center, Iowa for the period July 1, 2014 through June 30, 2015