Combinatorial peptide library-based identification of peptide ligands for tumor-reactive cytolytic T lymphocytes of unknown specificity.

A novel approach for the identification of tumor antigen-derived sequences recognized by CD8(+) cytolytic T lymphocytes (CTL) consists in using synthetic combinatorial peptide libraries. Here we have screened a library composed of 3.1 x 10(11) nonapeptides arranged in a positional scanning format, in a cytotoxicity assay, to search the antigen recognized by melanoma-reactive CTL of unknown specificity. The results of this analysis enabled the identification of several optimal peptide ligands, as most of the individual nonapeptides deduced from the primary screening were efficiently recognized by the CTL. The results of the library screening were also analyzed with a mathematical approach based on a model of independent and additive contribution of individual amino acids to antigen recognition. This biometrical data analysis enabled the retrieval, in public databases, of the native antigenic peptide SSX-2(41-49), whose sequence is highly homologous to the ones deduced from the library screening, among the ones with the highest stimulatory score. These results underline the high predictive value of positional scanning synthetic combinatorial peptide library analysis and encourage its use for the identification of CTL ligands.

Making a Library Beginning : How a Library May Be Started in a Small Town (Iowa Library Commission Leaflet No. 11)

A short booklet from the Iowa Library Commission containing advice on how small towns can start and set up public libraries for their communities.

Building the future: the Brazilian university library in 2010

Information technology will affect academic activities as well as the nature of the high education sector. This sector besides the need to assimilate these technologies will need to attend the requisites of market globalization and, as consequence, all theses changes will be reflected in the university library. Prospectives impacts will affect the structure (emphasis in user services, outsourcing of several services), in the financing aspect (growing of consortia in order to reduce costs), in services (electronic reference, support to long distance education programs, intelligent agents) and in the clientele (attending the great demand por high education which implies a diversity of people).

University of Iowa School of Library and Information Science newsletter. 1994-1995, no. 36.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. 1995-1996, no. 37.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. 1996-1997, no. 38.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. 1998-1999, no. 40.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. Spring 2000, no. 41.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. Spring 2001, no. 42.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. Spring 2002, no. 43.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. Fall 2007, no. 46.

Newsletter from the University of Iowa School of Library and Information Science.

University of Iowa School of Library and Information Science newsletter. Fall 2009, no. 47.

Newsletter from the University of Iowa School of Library and Information Science.