Catalogue d'estampes anciennes et modernes... par suite du décès de M. J. [Jean] Bein... vente... 26 avril 1858 / [expert] Vignères

[Vente. Estampes. 1858-04-26. Paris]

Crystal packing modifies ligand binding affinity: the case of aldose reductase.

The relationship between the structures of protein-ligand complexes existing in the crystal and in solution, essential in the case of fragment-based screening by X-ray crystallography (FBS-X), has been often an object of controversy. To address this question, simultaneous co-crystallization and soaking of two inhibitors with different ratios, Fidarestat (FID; K(d) = 6.5 nM) and IDD594 (594; K(d) = 61 nM), which bind to h-aldose reductase (AR), have been performed. The subatomic resolution of the crystal structures allows the differentiation of both inhibitors, even when the structures are almost superposed. We have determined the occupation ratio in solution by mass spectrometry (MS) Occ(FID)/Occ(594) = 2.7 and by X-ray crystallography Occ(FID)/Occ(594) = 0.6. The occupancies in the crystal and in solution differ 4.6 times, implying that ligand binding potency is influenced by crystal contacts. A structural analysis shows that the Loop A (residues 122-130), which is exposed to the solvent, is flexible in solution, and is involved in packing contacts within the crystal. Furthermore, inhibitor 594 contacts the base of Loop A, stabilizing it, while inhibitor FID does not. This is shown by the difference in B-factors of the Loop A between the AR-594 and AR-FID complexes. A stable loop diminishes the entropic energy barrier to binding, favoring 594 versus FID. Therefore, the effect of the crystal environment should be taken into consideration in the X-ray diffraction analysis of ligand binding to proteins. This conclusion highlights the need for additional methodologies in the case of FBS-X to validate this powerful screening technique, which is widely used.

Estudio de la edad, el crecimiento, la madurez sexual y el desove de la anchoveta (Cetengraulis Mysticetus) en el Golfo de Panamá

Realiza un estudio sobre la edad, crecimiento, madurez sexual y las épocas de desove de la anchoveta. Estas investigaciones se realizaron en el Golfo de Panamá entre los años de junio de 1951 a enero de 1956.

Estudio de la edad, el crecimiento, la madurez sexual y el desove de la anchoveta (Cetengraulis Mysticetus) en el Golfo de Panamá (Conclusión)

Presenta los métodos utilizados en los laboratorios para determinar la madurez sexual y desove de la población de anchovetas, su época y frecuencia. Estos datos concluyen la edad con relación al tiempo del desove anual gracias al índice de gónadas recolectado en el Golfo de Panamá.

Report of the Section of Agencies and Institutions Division of Child Welfare State Department of Social Welfare, 1943

History of child and social welfare in the State of Iowa including legal responsibilities, rules and regulations, inspections and licensing and supervision. it also covers cooperation with state agencies. It also describes in detail the histories, functions and problems of individual welfare homes and schools.

Genome-wide association analysis of blood-pressure traits in African-ancestry individuals reveals common associated genes in African and non-African populations.

High blood pressure (BP) is more prevalent and contributes to more severe manifestations of cardiovascular disease (CVD) in African Americans than in any other United States ethnic group. Several small African-ancestry (AA) BP genome-wide association studies (GWASs) have been published, but their findings have failed to replicate to date. We report on a large AA BP GWAS meta-analysis that includes 29,378 individuals from 19 discovery cohorts and subsequent replication in additional samples of AA (n = 10,386), European ancestry (EA) (n = 69,395), and East Asian ancestry (n = 19,601). Five loci (EVX1-HOXA, ULK4, RSPO3, PLEKHG1, and SOX6) reached genome-wide significance (p < 1.0 × 10(-8)) for either systolic or diastolic BP in a transethnic meta-analysis after correction for multiple testing. Three of these BP loci (EVX1-HOXA, RSPO3, and PLEKHG1) lack previous associations with BP. We also identified one independent signal in a known BP locus (SOX6) and provide evidence for fine mapping in four additional validated BP loci. We also demonstrate that validated EA BP GWAS loci, considered jointly, show significant effects in AA samples. Consequently, these findings suggest that BP loci might have universal effects across studied populations, demonstrating that multiethnic samples are an essential component in identifying, fine mapping, and understanding their trait variability.

[Lettre autographe signée de Giuseppina Verdi adressée à Escudier, Sant'Agata, 19 août 1858]

En français

[Lettre autographe signée de Giuseppe Verdi adressée à Escudier, Gênes, 20 octobre 1858]

Post-scriptum en français de Giuseppina Verdi