Deuterium Equilibrium Time in Saliva of Newborn Infants

There is an increasing interest about the use of stable isotopes for body composition analysis in pediatrics. To ensure the success of total body water analysis by the deuterium dilution method, it is fundamental to determine the equilibrium tune (plateau) of deuterium in the body fluid studied. Objectives: We report here the equilibration time of deuterium oxide in the saliva of newborns after oral intake of the isotope. Methods: Twenty healthy term newborn infants, 10 males and 10 females, were analyzed. Saliva was collected from each newborn before the oral administration of a 100 mg/kg dose of deuterium oxide (baseline sample) and then at 1-hour intervals for 5 hours after administration. Deuterium enrichment of saliva was determined by isotope ratio mass spectrometry according to the recommendations of the International Atomic Energy Agency. Results: The plateau time of deuterium in saliva occurred 3 hours after oral administration of the stable isotope. Conclusion: These data are essential for further studies on the body composition of newborn infants. To the best of our knowledge, this is the first study regarding the equilibration time of deuterium in the saliva of term newborns. JPGN 48:471-474, 2009.

Effects of the utilization of homeopathic elements in commercial diluent on swine sperm viability

It has been speculated that the homeopathic treatment of sperm cells in order to improve semen quality could be promising. However, few data is available and its use in spermatozoa requires investigation. It is well established that mitochondrial membrane potential is an important viability parameter of spermatozoa and it is intimately related to reproductive efficiency. In this manner, new technologies in order to improve the activity of sperm cells and, finally, the fecundity of swine herds are of extremely importance. Due to the lack of knowledge of homeopathic treatment effect on spermatozoa, the aim of the present study was to verify the effect of three different homeopathic treatments on viability of boar sperm cells. Three homeopathic treatments composed by Pulsatila CH6, Pulsatila and Avena CH6, Avena CH6 and one control treatment (sucrose) were added to diluted boar semen, which were cooled for 24 or 48 h. Interestingly, no positive effect of homeopathic treatments was observed over semen viability. However, it was demonstrated that the 24 h of cooling storage provided more viable sperm cells when compared to the 48-h period. This effect of storage period on sperm viability was assessed by intact plasmatic membrane, intact acrosome and mitochondrial membrane potential evaluation.

Residents’ perceptions of tourism impacts in Guimarães (Portugal): a cluster analysis

In this investigation, a cluster analysis was used to separate Guimara˜es (Portugal) residents into clusters according to their perceptions of the impacts of tourism development. This approach is uncommonly applied to Portugal data and is even rarer for world heritage sites. The world heritage designation is believed to make an area more attractive to tourists. The clustering procedure analysed 400 data observations from a Guimara˜es resident survey and revealed the existence of three clusters: the Sceptics, the Moderately Optimistic and the Enthusiasts. The results were consistent with the empirical literature’s results, with the emergent nature of the destination found to be relevant. The fact that tourism is relatively recent in this destination has its major reflex in the devaluation by most of the residents of the negative impacts of tourism development.

Evaluating the Guimarães 2012 European Capital of Culture (ECOC): a Tourist Perception Approach

Guimarães has hosted, during the year of 2012, one of the European Capital of Culture (ECOC). The evaluation of the event is needed as public, private and community funds are involved. The approach considers the tourists (domestic and international) as external and independent stakeholders who assessed the cultural activities developed during the event and the attributes of the city. The results of the survey show that hosting the 2012 ECOC was a major contribution for attracting new visitors to the city, although many of them just for a short period of time. The main source of general information collected by tourists was the Internet, and the traditional media. Only a small amount of respondents demonstrated a specialized knowledge of the cultural program. The most cited and appreciated performances came from the areas of music, exhibitions, and theatre. According to the perceptions of tourists, the tangible assets were clearly detached from the set of attributes of Guimarães, including buildings, churches and chapels, whereas intangible assets were less noted. Overall, Guimarães received a very positive evaluation related to city image and stay and is highly recommended by tourists to friends and relatives.

Potenciais impactos para Guimarães do acolhimento da Capital Europeia da Cultura 2012: uma análise baseada em experiências anteriores

A nomeação de Guimarães como Capital Europeia da Cultura (CEC), em 2012, suscitou grandes expectativas na comunidade local, nos territórios limítrofes e, de um modo geral, na população portuguesa. No entanto, muitos dos potenciais impactos são ainda pouco perceptíveis pela maior parte das pessoas, se bem que muitas delas antecipem que os impactos sejam positivos. A presente comunicação pretende aferir, de forma prospectiva, os possíveis impactos do acolhimento por Guimarães da CEC, em 2012. Por referência a experiências anteriores de organização de outras CEC, procura-se identificar alguns dos efeitos, em termos de atracção de turistas (nacionais e internacionais), das actividades culturais programadas, disponibilidades orçamentais e audiências, bem como aferir do potencial impacto económico e do legado do evento para o futuro da cidade. Com base na análise efectuada, pode concluir-se que Guimarães CEC 2012 oferece um potencial de atracção para o Norte do país e região da Galiza, possui um orçamento, por habitante, comparável a outras CEC e beneficia do empenhamento dos agentes locais. Menos positiva é a distância da cidade a dois dos principais destinos turísticos nacionais (Lisboa e Algarve), a extensão da programação cultural do evento, o fraco envolvimento dos agentes culturais locais no programa de actividades do evento e a inexistência de espaços museológicos de referência internacional. Em contrapartida, destaca-se a existência de ligações aéreas de baixo custo a várias cidades Europeias através do aeroporto do Porto, a possibilidade de reposicionar a imagem da cidade ao nível nacional e internacional, a disponibilização de fundos para a revitalização urbana do centro histórico e o aumento esperado do número de turistas (nacionais e estrangeiros). A recessão económica (nacional e Europeia), as dificuldades orçamentais, a pouca ambição da política de angariação de patrocínios e de recursos financeiros privados e a sustentabilidade do evento no futuro são algumas ameaças que poderão comprometer o sucesso de Guimarães CEC 2012.

Motivations to visit and image destination of Guimarães: differences between Portuguese and foreign tourists during 2012 Guimarães European Capital of Culture (ECOC)

Guimarães hosted the European Capital of Culture (ECOC) during the year of 2012. This study investigates the differences between Portuguese and foreign tourists regarding the main motivations to visit Guimarães and the retained perceived image of the destination. To achieve that purpose a survey was administered to 390 tourists that visited the city during the cultural event. The results show that tourists who visited Guimarães are relatively young, wealthy, employed and well educated. They are touring around the northern part of the country which includes an itinerary beginning in Porto, and extended to other important neighboring cities such as Braga or Viana do Castelo. The main motivations to visit the city, for both Portuguese and foreign tourists, are its historical heritage and the title of ECOC, the associated cultural events and celebrations that take place during 2012. However, these items were more valued by foreigners than Portuguese tourists. Using a factor analysis the tourists’ perceived attributes of Guimarães were described in four dimensions: “material heritage”, “intangible heritage”, “cultural performance”, and “sport and education”. Although foreigners and nationals perceived the tourism attributes of the city differently, the comparison of the mean scores of the four factors across Portuguese and foreigner tourists reveals that the most valued and least valued factors are common to both groups.

Cultural tourism in Northwest Portugal – the case of the world heritage site of Guimarães

A cidade de Guimarães, no noroeste português, é um espaço imbuído de um forte significado simbólico e cultural. A acreditação pela U.N.E.S.C.O. do seu centro histórico como Património Cultural da Humanidade, em Dezembro de 2001, contribuiu significativamente para aumentar o seu potencial em termos turísticos. Na realidade, desde aquela data, tem-se assistido a um aumento sustentado do número de visitantes. O presente capítulo debruça-se sobre a análise de alguns resultados de um inquérito por questionário aplicado aos residentes do município de Guimarães com o objetivo de avaliar a sua perceção dos benefícios que a atividade turística pode trazer. O inquérito foi implementado entre Janeiro e Março de 2010. Os resultados mostram que aqueles revelam uma perceção favorável dos impactos do turismo e que esta perceção está correlacionada com a idade e o nível de instrução dos inquiridos.

The host-tourist interaction in a world heritage site: the case of Guimarães

Guimarães, in the northwest of Portugal, is a city of strong symbolic and cultural significance and its nomination by UNESCO as world heritage, in 2001, enlarged its tourism potential. In this paper we present a few results of a survey that envisaged capturing the Guimarães residents’ perceptions of tourism impacts and their attitudes towards tourists. Specifically, one analyzes the type of relationship that exists between some socio-demographic groups and the perceived tourism impacts, as well as their socio-characteristics and the existing level of interaction between residents and tourists. The survey was implemented between January and March 2010 to a convenience sample of 540 inhabitants of the municipality of Guimarães resulting in 400 questionnaires with complete data. For this, we made use of various statistical techniques. Using a factorial analysis, we can conclude that the three factors used explain 52.3% of the variance contained in the original variables obtained from the survey. By another side, using a logit model in the analysis and taking as the dependent variable the frequent or very frequent contact with tourists, we found that only the variables referred to perceived positive impacts of tourism, education and the place of residence in urban areas have shown to be statistically significant. We are aware of the multiple ways the issue of residents’ perceptions and attitudes towards tourism can be approached and of the difficulties to get useful policy-oriented insights. This paper is a step in that trail.

Potenciais Impactos para Guimarães do Acolhimento da Capital Europeia da Cultura 2012: uma análise baseada em experiências anteriores

A nomeação de Guimarães (cidade do Noroeste português) como Capital Europeia da Cultura (CEC), em 2012, suscitou grandes expectativas na comunidade local, nos territórios limítrofes e, de um modo geral, na população portuguesa. No entanto, muitos dos potenciais impactos são ainda pouco perceptíveis pela maior parte das pessoas, se bem que muitas delas antecipem que os impactos sejam positivos. A presente comunicação pretende aferir, de forma prospectiva, os possíveis impactos do acolhimento por Guimarães da CEC, em 2012. Por referência a experiências anteriores de organização de outras CEC, procura-se identificar alguns dos efeitos, em termos de atracção de turistas (nacionais e internacionais), das actividades culturais programadas, disponibilidades orçamentais e audiências, bem como aferir do potencial impacto económico e do legado do evento para o futuro da cidade. Com base na análise efectuada, pode concluir-se que Guimarães CEC 2012 oferece um potencial de atracção para o Norte do país e região da Galiza, possui um orçamento, por habitante, comparável a outras CEC e beneficia do empenhamento dos agentes locais. Menos positiva é a distância da cidade a dois dos principais destinos turísticos nacionais (Lisboa e Algarve), a extensão da programação cultural do evento, o fraco envolvimento dos agentes culturais locais no programa de actividades do evento e a inexistência de espaços museológicos de referência internacional. Em contrapartida, destaca-se a existência de ligações aéreas de baixo custo a várias cidades Europeias através do aeroporto do Porto, a possibilidade de reposicionar a imagem da cidade ao nível nacional e internacional, a disponibilização de fundos para a revitalização urbana do centro histórico e o aumento esperado do número de turistas (nacionais e estrangeiros). A recessão económica (nacional e Europeia), as dificuldades orçamentais, a pouca ambição da política de angariação de patrocínios e de recursos financeiros privados e a sustentabilidade do evento no futuro são algumas ameaças que poderão comprometer o sucesso de Guimarães CEC 2012.

Residents’ perception of the benefits of cultural tourism: the case of Guimarães

In many cities throughout Europe and the rest of the world, cultural tourism plays a major role in local economic development. Tourism also acts to preserve cultural heritage, as it provides a way to acquire the revenue needed to restore and preserve historical buildings, and encourages local people to value their traditions and festivals. However, tourism success can be a double-edged sword with tourist flows having a strong impact on the quality of life of residents when certain tourist thresholds are surpassed. This paper aims to address local residents’ perception of the benefits of cultural tourism. To capture their perception, a survey was implemented, addressed to both the city centre inhabitants and the non-urban residents of the Guimarães municipality.

Tourists’ perceptions of world heritage destinations: the case of Guimarães

Guimarães is a world heritage site (UNESCO) since December 2001, and is hosting the European Capital of Culture (ECOC) in 2012. This paper examines the profile, destination image and motivations of tourists’ visiting Guimarães before the cultural event. Based on survey responses from 276 tourists, this study found that tourists arrived to Guimarães came from the two most important cities in the northern part of Portugal (Porto and Braga). They are relatively young and well educated compared with the average tourists that visited Portugal. The results suggest that many tourists are aware of the city status as a world heritage site encompassing a historic centre, monuments, and architectural buildings. Further, these perceptions shape the image of Guimarães, as the factor analysis indicates that “historical background and functionality” is the most reliable and valid factor behind the choice of visiting the city. Finally, the main tourists’ motivation to choose Guimarães as theirs destination is educational, rather than recreational as they want to live a learning experience.

Avaliação ex-ante, pelos media, de Guimarães 2012 capital europeia da Cultura

A nomeação de Guimarães como Capital Europeia da Cultura (CEC) em 2012 veio colocar na ordem do dia a necessidade de medir os efeitos que a concretização deste megaevento irá provocar na cidade e no município. A conveniente ponderação dos benefícios e dos custos e um alargado envolvimento da comunidade tendem a permitir encontrar formas de reduzir os impactos negativos e aumentar os impactos positivos. A presente comunicação analisa o envolvimento da população e das associações locais no planeamento e organização da Capital Europeia da Cultura 2012, aproximados pela via da cobertura feita pela imprensa local e nacional à preparação do megaevento. A análise de conteúdo das notícias publicadas abrangeu o período que medeia entre Janeiro e Dezembro de 2011 e respeitou a dois semanários locais e um diário nacional. Com base na análise efetuada, pode concluir-se que foi fraco o envolvimento da população e dos agentes culturais na organização da CEC 2012 e que existiu uma forte reação negativa ao modelo de planeamento do megaevento conduzido pela estrutura organizadora oficial, o que veio pôr em causa a desejável adesão dos vimaranenses ao mesmo e, por essa via, o respetivo sucesso, sobretudo numa perspetiva de efeitos a médio e longo prazo.

Guimarães residents’ perceptions towards tourism impacts: a cluster analysis

The present study was designed to assess and segment local residents with respect to their perceived impacts of Guimarães tourism development. The residents of this municipality (located in the northern part of Portugal) are quite strong in their support to tourism. However, they do not keep a homogeneous perception of tourism impacts. A clusters analysis using data from a survey of 400 Guimarães residents’ has revealed the existence of three clusters, according the different degrees of perceived tourism impacts: the Skeptics - moderate in relation to the benefits (averages range from 2.89-3.74) and the ones more concerned with its costs (averages range from 2.86-3.74); the Moderately optimistic - very optimistic about the benefits of tourism (averages range from 3.74-4.51) and conscious of the costs (averages range from 2.71-3.49); the Enthusiasts - very optimistic about tourism benefits (averages range from 2.92-4.52) and little worried about its costs (averages range from 1.78-3.26). Following the data from the survey, the findings are discussed and a few conclusions are extracted.

Teores de nutrientes de dois cultivares de oliveira durante o crescimento vegetativo e o florescimento

Para o planejamento de adubações, visando a manter os níveis de nutrientes adequados para as plantas, é importante monitorar seu estado nutricional durante as diferentes fases de desenvolvimento, sendo a análise foliar o melhor método para avaliar os níveis de nutrientes na planta. Esta pesquisa foi realizada com o objetivo de avaliar os teores de nutrientes em dois cultivares de oliveira, nas fases de crescimento vegetativo e na floração. Foram avaliados espécimes dos cultivares 'Grappolo' e 'Barnea', com dois anos de idade, obtidos a partir do enraizamento de estacas, plantadas no espaçamento de 5 m entre linhas e 3 m entre plantas. Utilizou-se o esquema de parcela sub-dividida, tendo na parcela o fatorial 2 x 2, sendo duas cultivares e duas fases de emissão fenológicas: plantas com brotações vegetativa e reprodutivas, no delineamento de blocos casualizados, com cinco repetições e sete plantas por parcela. As amostras de folhas foram retiradas em quatro épocas: junho (na ocasião do plantio) e repetidas em agosto, setembro e outubro, quando as plantas iniciaram a emissão de brotações vegetativas e de flores. Na fase de florescimento, a oliveira apresentou menores teores de nutrientes que as plantas em fase vegetativa. O cultivar 'Barnea', por seu hábito de crescimento rápido, apresentou menores teores de nutrientes, durante as fases vegetativa e reprodutiva.

Perceptions of residents of hosting the “Guimarães 2012 European Capital of Culture”: An ex-ante approach

The European Capital of Culture is an annual mega-event, which can provide a good forum though which to challenge and engage local citizens, thus generating feelings of common citizenship. In addition, it presents an ideal opportunity to promote the restructuring of the hosting urban space. However, the success of both the organization and the city that hosts the cultural event depends on the residents’ commitment towards it, the consistency of the tourism attractions and activities supplied, and the capacity of anticipating and monitoring the evolution of tourists’ preferences. The present study aims to assess the intention to participate and the impacts perceived by residents of Guimarães from hosting one of the 2012 European Capitals of Culture (2012 ECOC) in the ex-ante period (2011). Through a convenience sample of 471 usable surveys applied to the local population, conducted between October and December 2011, we tried to identify some of these potential impacts. According to the results received, 40% of residents had a low or very low knowledge of the cultural program, although only 11% demonstrated no intention of attending any activity during the event. The comparison of the mean scores of the expected 2012 ECOC impacts by gender reveals that the most valued and least valued factors are common to female and male respondents. With regard to the differences between those that intended to attend the event and the ones that did not, expected participants rated positive and negative impact factors more than did the ones that did not intend to attend.