Thermal unfolding of human high-density apolipoprotein A-1: implications for a lipid-free molten globular state.

Apolipoprotein A-1 (apoA-1) in complex with high-density lipoprotein is critically involved in the transport and metabolism of cholesterol and in the pathogenesis of atherosclerosis. We reexamined the thermal unfolding of lipid-free apoA-1 in low-salt solution at pH approximately 7, by using differential scanning calorimetry and circular dichroism. At protein concentrations <5 mg/ml, thermal unfolding of apoA-1 is resolved as an extended peak (25 degrees C-90 degrees C) that can be largely accounted for by a single reversible non-two-state transition with midpoint Tm 57 +/- 1 degree C, calorimetric enthalpy deltaH(Tm)= 200 +/- 20 kcal/mol (1 kcal = 4.18 kJ), van't Hoff enthalpy deltaHv(Tm) approximately 32.5 kcal/mol, and cooperativity deltaHv(Tm)/deltaH(Tm) approximately 0.16. The enthalpy deltaH(Tm) can be accounted for by melting of the alpha-helical structure that is inferred by CD to constitute approximately 60% of apoA-1 amino acids. Farand near-UV CD spectra reveal noncoincident melting of the secondary and tertiary structural elements and indicate a well-defined secondary structure but a largely melted tertiary structure for apoA-1 at approximately 37 degrees C and pH 7. This suggests a molten globular-like state for lipid-free apoA-1 under near-physiological conditions. Our results suggest that in vivo lipid binding by apoA-1 may be mediated via the molten globular apolipoprotein state in plasma.

ESMO consensus guidelines for the management of patients with metastatic colorectal cancer

Colorectal cancer (CRC) is one of the most common malignancies in Western countries. Over the last 20 years, and the last decade in particular, the clinical outcome for patients with metastatic CRC (mCRC) has improved greatly due not only to an increase in the number of patients being referred for and undergoing surgical resection of their localised metastatic disease but also to a more strategic approach to the delivery of systemic therapy and an expansion in the use of ablative techniques. This reflects the increase in the number of patients that are being managed within a multidisciplinary team environment and specialist cancer centres, and the emergence over the same time period not only of improved imaging techniques but also prognostic and predictive molecular markers. Treatment decisions for patients with mCRC must be evidence-based. Thus, these ESMO consensus guidelines have been developed based on the current available evidence to provide a series of evidence-based recommendations to assist in the treatment and management of patients with mCRC in this rapidly evolving treatment setting.

UM Women's Soccer Team, 1996

First Row: Michele Brach, Debbie Flaherty.

Second Row: Ashley Marks, Amanda Gauthier, Stephanie McArdle, Alana Peters, Laura Fedrigo, Amber Berendowski, Karen Montgomery, Jaime Ross, Carrie Brady, Vanessa Lewis, Bethany Greenblatt, Jessica Limauro, Assistant Coach Scott Forrester.

Third Row: Trainer Ann Lighthill, Trainer Rex Thompson, Kristin Buckley, Kjersten Kuhlman, Kelly Lukasik, Mari Hoff, Emily Schmitt, Luren Glister, Jori Welchans, Jessica Jones, Marie Spaccarotella, Jen Stahl, Nicole Savage, Shannon Poole, Ruth Poulin, Assistant Coach Pete Kowall, Head Coach Debbie Belkin.

Development of materials and public relations efforts to promote child passenger safety. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Judicial perspectives on child passenger protection legislation. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

International passive architectural survey ; announcement of results /

"Prepared Under Task No. 6324.40."

Use of telephone surveys to determine awarenss of Tennessee's child passenger protection law. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Reisenovellen.

Mode of access: Internet.

Flue-cured tobacco, mechanization and labor : impacts of alternative production levels /

Mode of access: Internet.

Philippi a Limborch De veritate religionis Christianæ : amica collatio cum erudito Judæo.

"Urielis Acosta Exemplar humanae vitae": p. [651]-692.

An alphabetical digest of the public statute law of South-Carolina,

"The two first volumes contain all the English statutes and acts of Assembly of a general permanent nature ... also the constitutions of the state, and of the United States, together with such acts of the Congress of the United States, now in force, as more immediately concern the people ... of the state. These laws are digested under various heads or titles, and arranged alphabetically ... The third volume contains, under various titles, in alphabetical order, all the acts of Assembly, now of force, not contained in the two first volumes, being of a less general and permanent nature."--Introd.

The message of the Book of Revelation,

Mode of access: Internet.