Estudo in vivo da ação do tratamento endodôntico sobre endotoxinas bacterianas em canais radiculares com polpa necrosada e avaliação dos efeitos citotóxicos do conteúdo do canal radicular

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo in vitro das micro deformações ao redor de implantes suportes de próteses parciais fixas submetidos à cargas axiais e não axiais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação in vitro das alterações do esmalte humano, efetividade clínica e sensibilidade pós-clareamento dentário

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Candida dubliniensis: prevalência na cavidade bucal de pacientes diabéticos, com câncer de mama e hansenianos e patogenicidade experimental

O objetivo deste estudo foi avaliar a presença de C. dubliniensis dentre isolados bucais de pacientes com Diabetes mellitus do tipo I (n=39) e II (n=36), hansenianos (n=38) e sob quimioterapia para o câncer de mama (n=30) e de respectivos indivíduos controle pareados quanto à idade, gênero e condições bucais. Um total de 479 isolados previamente identificados por testes fenotípicos (formação de tubo germinativo, produção de hifas/pseudohifas/clamidoconídeos, fermentação e assimilação de carboidratos) e identificadas como C. albicans/C. dubliniensis foram incluídos no estudo. A existência de C. dubliniensis dentre os isolados foi analisada usando protocolo validado de PCR multiplex. Foi também realizado estudo de patogenicidade experimental utilizando camundongos em modelo de infecção sistêmica, objetivando comparar a virulência e cinética de infecção de C. dubliniensis com outras espécies do gênero Candida. Um isolado (0.002%) de C. dubliniensis foi detectado entre os isolados do grupo controle. Esta espécie não foi encontrada dentre os isolados dos outros grupos de pacientes. C. dubliniensis foi menos virulenta para camundongos em relação a C. albicans e C. tropicalis e mais virulenta do que C. krusei. O estudo da cinética de infecção mostrou infecção persistente no rim e no fígado mesmo após 21 dias da inoculação de C. dubliniensis. Conclui-se que a detecção de C. dubliniensis dentre os isolados clínicos foi baixa e observada apenas no grupo controle. C. dubliniensis foi menos virulenta para camundongos que C. albicans e C. tropicalis e causou infecção prolongada no rim e no fígado

Estudo da influência das características químicas e microestruturais nas propriedades mecânicas de sistemas totalmante cerâmicos

Pós-graduação em Odontologia Restauradora - ICT

Avaliação das correlações existentes entre experiência de cárie, acúmulo de placa bacteriana e nível de Iga anti-streptococcus mutans na saliva de crianças com dentição mista

Pós-graduação em Odontologia Restauradora - ICT

Influência do tratamento de superfície na infiltração marginal de reparos em porcelana

Pós-graduação em Odontologia Restauradora - ICT

Estudo in vivo e in vitro do vedamento marginal em restaurações de resina composta em cavidades de classe V

Pós-graduação em Odontologia Restauradora - ICT

Staphylococcus spp. Na cavidade bucal e na bolsa periodontal de indivíduos com periodontite crônica: presença e sensibilidade aos antibióticos

Staphylococcus are not usually studied in the oral cavity, when this happens, they are considered to belong to transitory microflora. Individuals that present periodontal disease represent possibles reservoirs of these opportunist bacteria in the oral cavity. The use of antibiotics whether for treatment of periodontal disease or due to hospital infections, may predispose the increase of the Staphylococcus spp. in the oral cavity because they easily become resistant to antibiotics, resulting in superinfection. The study was made with 88 patients, minimum age- 25 years old, presenting chronical periodontitis, with, at least, two sites having a probing pocket bigger or equal to 5mm. After anamnese and clinical periodontal examination samples were taken from the periodontal pocket using paper cones and from the oral cavity using mouth rinse. Of the total patients 37,50% presented Staphylococcus spp. in the periodontal pocket and 61,36% in lhe oral cavity; 27,27% presented bacteria in the two sites, not necessarily of the same specie. S. epidermidis was the most prevailing specie in periodontal pocket (15,9%) and oral cavity (27,27%). Positive for S. aureus in the periodontal pocket were 4,5% and for the oral cavity 25%, and 3,4% were positive for the two sites. There was not found significative statistical difference referring to the presence of the microorganisms as to age, smoking habit and increase of the probing depth. The majority of the isolated Staphylococcus samples showed resistance to the tested antibiotics, indicating that the drugs as an adjunct to periodontal therapy, must be seen with caution

Produção de anticorpo monoclonal reativo com leveduras do gênero candida

The aim of this study was to obtain a reactive monoclonal antibody against Candida albicans. Spleen celIs of BALB/c mice previously immunized withCandida were fused in vitro with mielorna cells Sp2-0Ag14. The resultant hybridcells were kept in culture medium at 5% C02.The suspension growing cells were tested by ELISA to check the antibodies titer. The positive colonies were cloned to achieve the monoclonal antibody and further expanded in mice peritoneum. The antibody produced was purified and isotope. The monoclonal antibody was denominated 76C. The 76C was directed against mannoprotein molecules from Candida cell surface, which has not yet been completely investigated to find outlhe specific nature of epitope. The antibody 76C was analyzed by DOT BLOTagainst different Candida species and there were positives results in 87,5% of the tested samples_ The monoclonal antibody 76C will be a useful tool to investigate oligomannoside epitope from mannan and could be applied in sera diagnosis in invasive candidiasis and in studies of glicidic epitope.

Influência da calcitonina na regeneração óssea em mandíbulas de ratas ovariectomizadas

In This work we studied the action of calcitonin on bone regeneration in the jaws of normal and ovariectomized rats with and without administration of calcitonin. One hundred rats aged approximately sixty days were used for this study. Of these fifty were ovariectomizedOv forming group and one month after the surgery, animals in all a hundred surgical bone defect was made in the angle of the mandible, creating a bone lesion of approximately 4 mm, covered with a PTFE barrier. OvThe animals were divided into two equal groups: Ov (ovaricctomizados) and LMO (ovariectomized with calcitonin). The other fifty animals, only with the bone lesion were also divided into two groups: control (C) and CM (control with calcitonin). Treated groups received the first dose of the substance immediately after surgery, the dose of 2 IU / kg IM three times per week. The animals were sacrificed after three, seven, 14, 21 and 28 days, with their jaws removed and sent for histological preparation. Densitometric analyzes were performed histological and histomorphometric verifying that it was observed that the Ov animals showed decreased bone density and a much slower regenerative process, when compared with control animals. Calcitonin proved effective in both ovariectomized animals and in those with only bone injury.

Análise química, microestrutural, determinação das propriedades mecânicas e dureza de ligas odontológicas (Ag Pd,Co Cr e NiCr) fundidas em diferentes temperaturas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo comparativo da textura superficial entre os materiais estéticos indiretos

The purpose of this study was to evaluate surface rugosity among indirect esthetic materials (Vita VMK 95, Solidex, Artglass, Targis). Ten samples of each material in the cilindric shape with dimension 6 x 6 mm were manufactured. The surface were submitted to finishing and polishing, according to manufacturer's recommendations. The samples were submitted to measuremenst, using the Hommel Tester T500 device. The average values of maxim surface rugosity among the materiais ín question, were 5, 73pm; 5, 15pm; 6, 83 j.Jm e 3, 72 j.Jm, respectively. Sample were also subjectted to variance analysis- ANOVA (5%), had detected significance. Concluded that the material Solidex did not present significant statistical differences among groups, while Targis presented significant statistical differences in relation to the Artglass and the ceramic Vita vmk95.

Estudo clínico comparativo entre sutura convencional e vaporização a laser de dióxido de carbono (CO2) em pele de ratos

The proposal of this study was to compare a wound healing between conventional suture and vaporization with CO2 laser in rats skin. ln such case 24 rats were utilized, making two longitudinal incisions in dorsum by scalpel. ln the left side incision the wound was sutured with nylon thread. ln the right side incision the wound were close by approach and vaporized with C02 laser, defocusing, 8 watts in continuous mode. The wound healing was followed by clinical photographic taking at the times: immediately after the procedure, 24 hours, 3, 7, 14, and 21 days. The results showed an early delay of reparation in healing vaporized in comparison with the sutured one, however occurred 21 days both repair tissue showed the same clinical characteristic. This results suggested that the C02 laser can be used by eventual substitute of suture.

Estimativa do surto de crescimento puberal pela avaliação das vértebras cervicais em radiografias cefalométricas laterais

The aim of this research was to estimate the skeletal growth and development by observingradiographically the morphological changes of the first, second and third cervical vertebrae, according to the method proposed by Hassel &Farman32 (1995) in patients who were going through the outbreak of pubertal growth. The radiographic inspection of the cervical vertebrae was performed by means of cephalometric radiographs and the outbreak of pubertal growth was identified through the events of ossification of the hand and wrist. For that purpose, we selected thecephalometric radiographs and hand•wrist radiographs of 110 Brazilianleukoderms, male and female,with chronological age ranging from 8 to 14,6 years old for the girls and from 9,5 to 15,4 years old for the boys.The results showed that there was a statistically significant correlation between the maturation indicators of the cervical vertebrae and the patients who were going through the outbreak of pubertal growth. The conclusion was that the radiographic evalution of the changes of the cervical vertebrae, in the cephalometric radiographs, is an alternative parameter, which is reliable and practical in the skeletal evalution, and the it completes the range of information that must be obtained from the patient under orthodontic treatment and that it can, circumstantially, replace other methods of evalution